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PR01155

Identifier
VIP2RECEPTOR  [View Relations]  [View Alignment]  
Accession
PR01155
No. of Motifs
10
Creation Date
23-MAY-1999
Title
Vasoactive intestinal peptide receptor 2 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00249 GPCRSECRETIN
PRINTS; PR00491 VASOACTVEIPR
INTERPRO; IPR002284
GCRDB; GCR_1803; GCR_1834; GCR_1886; GCR_0794; GCR_1034; GCR_1028

GCRDB; GCR_1856
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.   
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.  
Molecular cloning and expression of a cDNA encoding the secretin receptor.
EMBO J. 10 1635-1641 (1991).
 
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H.,
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor.
SCIENCE 254 1022-1024 (1991). 
 
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F.,
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
 
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
 
6. WATSON, S. AND ARKINSTALL, S.
Vasoactive intestinal polypeptide family.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.278-283.
 
7. WEI, Y. AND MOJSOV, S.
Tissue specific expression of different human receptor types for pituitary
adenylate cyclase activating polypeptide and vasoactive intestinal 
polypeptide: implications for their role in human physiology.
J.NEUROENDOCRINOL. 8 811-817 (1996). 
 
8. DELGADO, M., MARTINEZ, C., JOHNSON, M.C., GOMARIZ, R.P. AND GANEA, D.
Differential expression of vasoactive intestinal peptide receptors 1 and 2
J.NEUROIMMUNOL. 68 27-38 (1996). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that   
encompasses a wide range of functions (including various autocrine, para-   
crine and endocrine processes). They show considerable diversity at the   
sequence level, on the basis of which they can be separated into distinct   
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,   
but between which there is no statistically significant similarity in   
sequence [1]. The currently known clan members include the rhodopsin-like   
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating   
pheromone receptors, and the metabotropic glutamate receptor family.       
 
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid   
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-   
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a manner
reminiscent of the rhodopsins and other receptors believed to interact with
G proteins. However, while a similar 3D framework has been proposed to
account for this, there is no significant sequence identity between these
families: the secretin-like receptors thus bear their own unique `7TM'
signature.      
 
Vasoactive intestinal polypeptide (VIP) has a wide physiological profile.
In the periphery, it induces relaxation in smooth muscle; inhibits
secretion in certain tissues, but stimulates secretion in others; and
modulates activity of cells in the immune system [6]. In the CNS, it has a
range of both excitatory and inhibitory actions. VIP receptors are
distributed widely in the periphery, and occur throughout the gastro-
intestinal tract and genitourinary system, other smooth muscles and
secretory glands. In the CNS, they are found abundantly in, e.g. the cortex,
hippocampus and thalamus [6]. All VIP receptors activate adenylyl cyclase.
 
There are two structurally distinct receptors that recognise VIP peptides
and pituitary adenylate cyclase activating polypeptide (PACAP) with similar
affinities (PACAP/VIPR-1, PACAP/VIPR-2), as well as a specific receptor for
the PACAP peptide (PACAP-1). RNA transcripts for all three receptor types
are found in human heart, brain and adipose tissue [7]. By contrast with
VIPR-1, which is constitutively expressed, the expression of VIPR-2 is 
induced only following stimulation through the TCR-associated CD3 complex [8].
VIP induces the expression of the VIPR-2 gene in the absence of additional
stimuli. Differential expression and regulation of the two VIP receptors in
T lymphocytes suggests different physiological roles in mediating the 
immunomodulatory activities of VIP and related neuropeptides [8]. 
 
VIP2RECEPTOR is a 10-element fingerprint that provides a signature for 
vasoactive intestinal peptide type 2 receptors. The fingerprint was derived
from an initial alignment of 3 sequences: the motifs were drawn from 
conserved regions spanning the full alignment length, focusing on those
areas that characterise the type 2 VIP receptors but distinguish them from
the rest of the VIP receptor family - motifs 1-5 lie in the N-terminal
region immediately preceding TM domain 1; motif 6 spans the loop between
TM domains 2 and 3; motif 7 lies in the loop between TM domains 3 and 4; 
motif 8 spans the loop between TM domains 6 and 7; and motifs 9 and 10 
reside at the C-terminus. A single iteration on SPTR37_9f was required to
reach convergence, no further sequences being identified beyond the 
starting set.
Summary Information
3 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
103333333333
90000000000
80000000000
70000000000
60000000000
50000000000
40000000000
30000000000
20000000000
12345678910
True Positives
VIPS_HUMAN    VIPS_MOUSE    VIPS_RAT      
Sequence Titles
VIPS_HUMAN  VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-R-2) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE III RECEPTOR) (PACAP TYPE III RECEPTOR) (PACAP-R-3) (HELODERMIN-PREFERRING VIP RECEPTOR) - HOMO SAPIENS (HUMAN). 
VIPS_MOUSE VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-R-2) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE III RECEPTOR) (PACAP TYPE III RECEPTOR) (PACAP-R-3) - MUS MUSCULUS (MOUSE).
VIPS_RAT VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-R-2) (PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE III RECEPTOR) (PACAP TYPE III RECEPTOR) (PACAP-R-3) - RATTUS NORVEGICUS (RAT).
Scan History
SPTR37_9f  1  50   NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
CWLLVRVSSIHPECRFHLE VIPS_RAT 11 11 -
CWLLVRVSSIHPECRFHLE VIPS_MOUSE 11 11 -
CWLLAPVNSIHPECRFHLE VIPS_HUMAN 12 12 -

Motif 2 width=20
Element Seqn Id St Int Rpt
QEEETKCAELLSSQMENHRA VIPS_RAT 31 1 -
QEEETKCAELLSSQTENQRA VIPS_MOUSE 31 1 -
QEEETKCAELLRSQTEKHKA VIPS_HUMAN 32 1 -

Motif 3 width=13
Element Seqn Id St Int Rpt
WRPADIGETVTVP VIPS_RAT 61 10 -
WRPADVGETVTVP VIPS_MOUSE 61 10 -
WRPANVGETVTVP VIPS_HUMAN 62 10 -

Motif 4 width=14
Element Seqn Id St Int Rpt
KVFSNFYSRPGNIS VIPS_RAT 76 2 -
KVFSNFYSRPGNIS VIPS_MOUSE 76 2 -
KVFSNFYSKAGNIS VIPS_HUMAN 77 2 -

Motif 5 width=15
Element Seqn Id St Int Rpt
EDESKITFYILVKAI VIPS_RAT 114 24 -
EDESKISFYILVKAI VIPS_MOUSE 114 24 -
EDESKITFYILVKAI VIPS_HUMAN 115 24 -

Motif 6 width=18
Element Seqn Id St Int Rpt
YSSSGTLRCHDQPGSWVG VIPS_RAT 183 54 -
YSSSGLLRCHDQPASWVG VIPS_MOUSE 183 54 -
YSSSGTLHCPDQPSSWVG VIPS_HUMAN 184 54 -

Motif 7 width=16
Element Seqn Id St Int Rpt
VAILPPSRCFLAYLLI VIPS_RAT 231 30 -
VAILPPSRCFLAYLLI VIPS_MOUSE 231 30 -
VAMLPPRRCFLAYLLI VIPS_HUMAN 232 30 -

Motif 8 width=12
Element Seqn Id St Int Rpt
PIGISSTYQILF VIPS_RAT 347 100 -
PIGISSTYQILF VIPS_MOUSE 347 100 -
PISISSKYQILF VIPS_HUMAN 348 100 -

Motif 9 width=16
Element Seqn Id St Int Rpt
WRGLCLTQPGSRDYRL VIPS_RAT 389 30 -
WRGLCLTQAGSRDYRL VIPS_MOUSE 389 30 -
WRSRCPTPSASRDYRV VIPS_HUMAN 390 30 -

Motif 10 width=24
Element Seqn Id St Int Rpt
SRNGSESALQIHRGSRTQSFLQSE VIPS_RAT 410 5 -
SRNGSESALQIHRGSRTQSFLQSE VIPS_MOUSE 410 5 -
SRNGSEGALQFHRGSRAQSFLQTE VIPS_HUMAN 411 5 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
CWLLVRVSSIHPECRFHLE VIPS_RAT 11 11 -
CWLLVRVSSIHPECRFHLE VIPS_MOUSE 11 11 -
CWLLAPVNSIHPECRFHLE VIPS_HUMAN 12 12 -

Motif 2 width=20
Element Seqn Id St Int Rpt
QEEETKCAELLSSQMENHRA VIPS_RAT 31 1 -
QEEETKCAELLSSQTENQRA VIPS_MOUSE 31 1 -
QEEETKCAELLRSQTEKHKA VIPS_HUMAN 32 1 -

Motif 3 width=13
Element Seqn Id St Int Rpt
WRPADIGETVTVP VIPS_RAT 61 10 -
WRPADVGETVTVP VIPS_MOUSE 61 10 -
WRPANVGETVTVP VIPS_HUMAN 62 10 -

Motif 4 width=14
Element Seqn Id St Int Rpt
KVFSNFYSRPGNIS VIPS_RAT 76 2 -
KVFSNFYSRPGNIS VIPS_MOUSE 76 2 -
KVFSNFYSKAGNIS VIPS_HUMAN 77 2 -

Motif 5 width=15
Element Seqn Id St Int Rpt
EDESKITFYILVKAI VIPS_RAT 114 24 -
EDESKISFYILVKAI VIPS_MOUSE 114 24 -
EDESKITFYILVKAI VIPS_HUMAN 115 24 -

Motif 6 width=18
Element Seqn Id St Int Rpt
YSSSGTLRCHDQPGSWVG VIPS_RAT 183 54 -
YSSSGLLRCHDQPASWVG VIPS_MOUSE 183 54 -
YSSSGTLHCPDQPSSWVG VIPS_HUMAN 184 54 -

Motif 7 width=16
Element Seqn Id St Int Rpt
VAILPPSRCFLAYLLI VIPS_RAT 231 30 -
VAILPPSRCFLAYLLI VIPS_MOUSE 231 30 -
VAMLPPRRCFLAYLLI VIPS_HUMAN 232 30 -

Motif 8 width=12
Element Seqn Id St Int Rpt
PIGISSTYQILF VIPS_RAT 347 100 -
PIGISSTYQILF VIPS_MOUSE 347 100 -
PISISSKYQILF VIPS_HUMAN 348 100 -

Motif 9 width=16
Element Seqn Id St Int Rpt
WRGLCLTQPGSRDYRL VIPS_RAT 389 30 -
WRGLCLTQAGSRDYRL VIPS_MOUSE 389 30 -
WRSRCPTPSASRDYRV VIPS_HUMAN 390 30 -

Motif 10 width=24
Element Seqn Id St Int Rpt
SRNGSESALQIHRGSRTQSFLQSE VIPS_RAT 410 5 -
SRNGSESALQIHRGSRTQSFLQSE VIPS_MOUSE 410 5 -
SRNGSEGALQFHRGSRAQSFLQTE VIPS_HUMAN 411 5 -