SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01097

Identifier
TRNSRECEPTRP  [View Relations]  [View Alignment]  
Accession
PR01097
No. of Motifs
3
Creation Date
04-MAR-1999  (UPDATE 10-JUN-1999)
Title
Transient receptor potential family signature
Database References
PRINTS; PR01642 TRPCHANNEL1; PR01643 TRPCHANNEL2; PR01644 TRPCHANNEL3
PRINTS; PR01645 TRPCHANNEL4; PR01646 TRPCHANNEL5; PR01647 TRPCHANNEL6
PRINTS; PR01648 TRPCHANNEL7
PRODOM; PD004194; PD003230; PD004174
INTERPRO; IPR002153
Literature References
1. ZHU, X., JIANG, M., PEYTON, M., BOULAY, G., HURST, R., STEFANI, E. 
AND BIRNBAUMER, L.
Trp, a novel mammalian gene family essential for agonist-activated 
capacitative Ca2+ entry.
CELL 85 661-671 (1996).
 
2. BOULAY, G., ZHU, X., PEYTON, M., JIANG, M., HURST, R., STEFANI, E. 
AND BIRNBAUMER, L.
Cloning and expression of a novel mammalian homolog of Drosophila transient 
receptor potential (Trp) involved in calcium entry secondary to activation 
of receptors coupled by the Gq class of G protein.
J.BIOL.CHEM. 272 29672-29680 (1997).

Documentation
Transient receptor potential (Trp) and related proteins are thought to be
Ca2+ ion channel subunits that mediate capacitative Ca2+ entry in response 
to a range of external and internal cell stimuli. Such Ca2+ entry is thought 
to be an essential component of cellular responses to many hormones and
growth factors, and acts to replenish intracellular Ca2+ stores that have
been emptied through the action of inositol triphosphate (IP3) and other 
agents. In non-excitable cells, i.e. those that lack voltage-gated Ca2+
channels, such as hepatocytes, this mode of Ca2+ entry is thought to be an
important step in generating the oscillations of intracellular Ca2+
concentration that characterise their response to stimulatory agents [1].
 
Studies on the visual transduction system in Drosophila led to the molecular
cloning of Trp and the cDNA of a related protein, Trp-like, which show
similarity to voltage gated Ca2+ channels in the regions known as S3 through
S6, including the S5-S6 linker that forms the ion-selective channel pore [2].
This provided evidence that Trp and/or related proteins might form mammalian
capacitative Ca2+ entry channels. They have varying lengths (usually 800-1000
amino acid residues), and hydropathy analyses suggest that they have six or
more transmebrane (TM) regions, flanked by cytosolic N- and C-termini.
 
TRNSRECEPTRP is a 3-element fingerprint that provides a signature for the
transient receptor potential family and related proteins. The fingerprint
was derived from an initial alignment of 26 sequences: the motifs were
drawn from the central hydrophobic region of the molecules containing the
putative TM domains. Two iterations on OWL31.1 were required to reach 
convergence, at which point a true set comprising 27 sequences was 
identified. A single partial match was also found, a TRP-like protein 
fragment from Mus musculus that matches motifs 1 and 2.
 
An update on SPTR37_9f identified a true set of 17 sequences.
Summary Information
17 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3171717
2000
123
True Positives
O18784        O18785        O35119        O35722        
O44872 O46129 O62826 O62852
P79100 Q13507 Q61056 Q61143
Q94447 TRP1_HUMAN TRPL_DROME TRP_DROME
YN52_CAEEL
Sequence Titles
O18784      TRP-1A - BOS TAURUS (BOVINE).                 
O18785 TRP-1B - BOS TAURUS (BOVINE).
O35119 TRANSIENT RECEPTOR POTENTIAL - RATTUS NORVEGICUS (RAT).
O35722 TRANSIENT RECEPTOR PROTEIN 1 (MTRP1 BETA) - MUS MUSCULUS (MOUSE).
O44872 R06B10.4 PROTEIN - CAENORHABDITIS ELEGANS.
O46129 TRP-LIKE PROTEIN - LOLIGO FORBESI (NORTHERN EUROPEAN SQUID).
O62826 TYROSINE-RELATED PROTEIN 2 - BOS TAURUS (BOVINE).
O62852 CAPACITATIVE CALCIUM ENTRY CHANNEL 2 - ORYCTOLAGUS CUNICULUS (RABBIT).
P79100 CAPACITATIVE CALCIUM ENTRY CHANNEL 1 (CAPACITATIVE CALCIUM ENTRY CHANNEL 1, TRUNCATED VARIANT BCCE1DELTA514) - BOS TAURUS (BOVINE).
Q13507 TRANSIENT RECEPTOR POTENTIAL RELATED CHANNEL 3 PROTEIN - HOMO SAPIENS (HUMAN).
Q61056 TRANSIENT RECEPTOR PROTEIN 1 (FRAGMENT) - MUS MUSCULUS (MOUSE).
Q61143 CALCIUM ENTRY CHANNEL - MUS MUSCULUS (MOUSE).
Q94447 TRP PROTEIN - CALLIPHORA VICINA (BLUE BLOWFLY) (CALLIPHORA ERYTHROCEPHALA).
TRP1_HUMAN Short transient receptor potential channel 1 (TrpC1) (TRP-1 protein) - Homo sapiens (Human).
TRPL_DROME TRANSIENT-RECEPTOR-POTENTIAL LIKE PROTEIN - DROSOPHILA MELANOGASTER (FRUIT FLY).
TRP_DROME TRANSIENT-RECEPTOR-POTENTIAL PROTEIN - DROSOPHILA MELANOGASTER (FRUIT FLY).
YN52_CAEEL HYPOTHETICAL 95.6 KD PROTEIN ZC21.2 IN CHROMOSOME III - CAENORHABDITIS ELEGANS.
Scan History
OWL31_1    2  200  NSINGLE    
SPTR37_9f 2 18 NSINGLE
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
GTYNVISLVVLLNMLIAMMNNS AB008889 580 580 -
GTYNVISLVVLLNMLIAMMNNS BTCCE1 604 604 -
GTYNVISLVVLLNMLIAMMNNS MMU50921 602 602 -
GTYNVISLVVLLNMLIAMMNNS MMU50922 602 602 -
GVYNVTMVIVLLNMLIAMINSS MMU49069 708 708 -
GTYNVISLVVLLNMLIAMMNNS AF029983 606 606 -
GTYNVISLVVLLNMLIAMMNNS MMAJ6204 606 606 -
GTYNVISLVVLLNMLIAMMNNS OCAJ6203 606 606 -
GTYNVISLVVLLNMLIAMMNNS AF029983 606 606 -
GTYNVISLVVLLNMLIAMMNNS BTA224862 91 91 -
GIFTIVMVIVLLNMLIAMITNS BTA6304 164 164 -
GTYNVVVVIVLTKLLVAMLHKS AF012900 627 627 -
GTYNVVVVIVLTKLLVAMLHKS MMU73625 643 643 -
GTYNVVVVIVLTKLLVAMLHKS AF012901 593 593 -
GTYNVVVVIVLTKLLVAMLHKS MMU95167 609 609 -
GTYNVVVVIVLTKLLVAMLHKS I38361 644 644 -
GIYNVTMVVVLLNMLIAMINSS JC5807 634 634 -
GSYSMIAIIILLNLLIAMMSNS LFAJ3320 606 606 -
IMYHCTSIIVLLNMLIAMMSHS YN52_CAEEL 662 662 -
GSYSVINIIVLLNMLIAMMSNS TRP_DROME 643 643 -
GSYSVINVIVLLNLLIAMMSNS TRPL_DROME 650 650 -
GTYNVVVVIVLTKLLVAMLHKS TRP1_HUMAN 627 627 -
GVYNVTMVIVLLNMLIAMINSS AF080394 709 709 -
GIYNVTMVVVLLNMLIAMINSS HSY13758 652 652 -
GSYSVINIIVLLNMLIAMMSNS DROTRPC 642 642 -
GSYSVINIIVLLNMLIAMMSNS CVTRP 640 640 -

Motif 2 width=14
Element Seqn Id St Int Rpt
YQLIADHADIEWKF MMU50922 624 0 -
FQEIEDDADVEWKF MMU49069 730 0 -
FQEIEDDADVEWKF AF080394 731 0 -
YQEIEDDSDVEWKF HSY13758 674 0 -
YQIISERADTEWKF DROTRPC 664 0 -
YQIISERADVEWKF CVTRP 662 0 -
YQLIADHADIEWKF AB008889 602 0 -
YQLIADHADIEWKF BTCCE1 626 0 -
YQLIADHADIEWKF MMU50921 624 0 -
YQLIADHADIEWKF AF029983 628 0 -
YQLIADHADIEWKF MMAJ6204 628 0 -
YQLIADHADIEWKF OCAJ6203 628 0 -
YQLIADHADIEWKF AF029983 628 0 -
YQLIADHADIEWKF BTA224862 113 0 -
FQKIEDAADVEWKF BTA6304 186 0 -
FQLIANHEDKEWKF AF012900 649 0 -
FQLIANHEDKEWKF MMU73625 665 0 -
FQLIANHEDKEWKF AF012901 615 0 -
FQLIANHEDKEWKF MMU95167 631 0 -
FQLIANHEDKEWKF I38361 666 0 -
YQEIEDDSDVEWKF JC5807 656 0 -
YQLIYSQADEEWKF LFAJ3320 628 0 -
FQLIANHEDKEWKF TRP1_HUMAN 649 0 -
YAMIDEHSDTEWKF TRPL_DROME 672 0 -
YQIISERADTEWKF TRP_DROME 665 0 -
FQIINDHADLEWKF YN52_CAEEL 684 0 -

Motif 3 width=14
Element Seqn Id St Int Rpt
TLPTPFNVIPSPKS MMU50922 652 14 -
TLPPPFNIIPSPKS AF029983 656 14 -
TLPPPFNIIPSPKS MMAJ6204 656 14 -
TLPPPFNIIPSPKS OCAJ6203 656 14 -
TLPPPFNIIPSPKS AF029983 656 14 -
TLPTPFNVIPSPKS BTA224862 141 14 -
TLPVPFNILPSPKA BTA6304 214 14 -
TLPPPFNIIPSPKT AF012900 677 14 -
TLPPPFNIIPSPKT MMU73625 693 14 -
TLPPPFNIIPSPKT AF012901 643 14 -
TLPPPFNIIPSPKT MMU95167 659 14 -
TLPPPFNIIPSPKT I38361 694 14 -
TLPPPLVPCPKSFV JC5807 684 14 -
TVPPPFNIIPSPKS LFAJ3320 656 14 -
TIPPPFNLCPNMKM DROTRPC 692 14 -
TLPPPFSLVPSPKS HSY13758 702 14 -
TLPVPFNLVPSPKS AF080394 759 14 -
TLPVPFNLVPSPKS MMU49069 758 14 -
TLPPPFNIIPSPKT TRP1_HUMAN 677 14 -
TLPPPFNVLPSVKW TRPL_DROME 700 14 -
TIPPPFNLCPNMKM TRP_DROME 693 14 -
SLPPPFNIIVTPKS YN52_CAEEL 712 14 -
TVPPPFNMFPSVKL CVTRP 690 14 -
TLPTPFNVIPSPKS AB008889 630 14 -
TLPTPFNVIPSPKS BTCCE1 654 14 -
TLPTPFNVIPSPKS MMU50921 652 14 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
GTYNVISLVVLLNMLIAMMNNS O62852 606 606 -
GTYNVISLVVLLNMLIAMMNNS O35119 580 580 -
GTYNVISLVVLLNMLIAMMNNS P79100 604 604 -
GTYNVVVVIVLTKLLVAMLHKS O18784 627 627 -
GTYNVVVVIVLTKLLVAMLHKS O18785 593 593 -
GTYNVVVVIVLTKLLVAMLHKS O35722 609 609 -
GTYNVVVVIVLTKLLVAMLHKS Q61056 627 627 -
GTYNVVVVIVLTKLLVAMLHKS TRP1_HUMAN 627 627 -
GSYCCCSIIVLLNMLIAMMSNS O44872 660 660 -
GVYNVTMVIVLLNMLIAMINSS Q61143 708 708 -
GSYSMIAIIILLNLLIAMMSNS O46129 606 606 -
GIYNVTMVVVLLNMLIAMINSS Q13507 652 652 -
GSYSVINIIVLLNMLIAMMSNS Q94447 640 640 -
GIFTIVMVIVLLNMLIAMITNS O62826 164 164 -
GSYSVINVIVLLNLLIAMMSNS TRPL_DROME 650 650 -
GSYSVINIIVLLNMLIAMMSNS TRP_DROME 643 643 -
IMYHCTSIIVLLNMLIAMMSHS YN52_CAEEL 662 662 -

Motif 2 width=14
Element Seqn Id St Int Rpt
YQLIADHADIEWKF O62852 628 0 -
YQLIADHADIEWKF O35119 602 0 -
YQLIADHADIEWKF P79100 626 0 -
FQLIANHEDKEWKF O18784 649 0 -
FQLIANHEDKEWKF O18785 615 0 -
FQLIANHEDKEWKF O35722 631 0 -
FQLIANHEDKEWKF Q61056 649 0 -
FQLIANHEDKEWKF TRP1_HUMAN 649 0 -
YQYISDQADIEWKF O44872 682 0 -
FQEIEDDADVEWKF Q61143 730 0 -
YQLIYSQADEEWKF O46129 628 0 -
YQEIEDDSDVEWKF Q13507 674 0 -
YQIISERADVEWKF Q94447 662 0 -
FQKIEDAADVEWKF O62826 186 0 -
YAMIDEHSDTEWKF TRPL_DROME 672 0 -
YQIISERADTEWKF TRP_DROME 665 0 -
FQIINDHADLEWKF YN52_CAEEL 684 0 -

Motif 3 width=14
Element Seqn Id St Int Rpt
TLPPPFNIIPSPKS O62852 656 14 -
TLPTPFNVIPSPKS O35119 630 14 -
TLPTPFNVIPSPKS P79100 654 14 -
TLPPPFNIIPSPKT O18784 677 14 -
TLPPPFNIIPSPKT O18785 643 14 -
TLPPPFNIIPSPKT O35722 659 14 -
TLPPPFNIIPSPKT Q61056 677 14 -
TLPPPFNIIPSPKT TRP1_HUMAN 677 14 -
TLPPPFNIVPSPKS O44872 710 14 -
TLPVPFNLVPSPKS Q61143 758 14 -
TVPPPFNIIPSPKS O46129 656 14 -
TLPPPFSLVPSPKS Q13507 702 14 -
TVPPPFNMFPSVKL Q94447 690 14 -
TLPVPFNILPSPKA O62826 214 14 -
TLPPPFNVLPSVKW TRPL_DROME 700 14 -
TIPPPFNLCPNMKM TRP_DROME 693 14 -
SLPPPFNIIVTPKS YN52_CAEEL 712 14 -