SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01081

Identifier
KCLTRNSPORT  [View Relations]  [View Alignment]  
Accession
PR01081
No. of Motifs
6
Creation Date
02-MAR-1999  (UPDATE 07-JUN-1999)
Title
K-Cl co-transporter signature
Database References
PRINTS; PR01082 KCLTRNSPORT1
PRODOM; PD009323; PD007491; PD009588
INTERPRO; IPR000076
Literature References
1. GILLEN, C.M., BRILL, S., PAYNE, J.A. AND FORBUSH, B. III.
Molecular cloning and functional expression of the K-Cl co-transporter from
rabbit, rat and human. A new member of the cation-chloride co-transporter 
family.
J.BIOL.CHEM. 271 16237-16244 (1996).
 
2. PAYNE, J.A. STEVENSON, T.J. AND DONALDSON, L.F.
Molecular characterization of a putative K-Cl co-transporter in rat brain.
A neuronal-specific isoform.
J.BIOL.CHEM. 271 16245-16252 (1996).
 
3. RIVERA, C., VOIPIO, J., PAYNE, J.A., RUUSUVUORI, E., LAHTINEN, H., 
LAMSA, K., PIRVOLA, U., SAARMA, M. AND KAILA, K.
The K+/Cl- co-transporter KCC2 renders GABA hyperpolarizing during neuronal
maturation.
NATURE 397 251-255 (1999).

Documentation
The K-Cl co-transporter (KCC) mediates the coupled movement of K+ and Cl- 
ions across the plasma membrane of many animal cells. This transport is
involved in the regulatory volume decrease in response to cell swelling in
red blood cells, and has been proposed to play a role in the vectorial
movement of Cl- across kidney epithelia. The transport process involves one
for one electroneutral movement of K+ together with Cl-, and, in all
known mammalian cells, the net movement is outward [1].
 
In neurones, it appears to play a unique role in maintaining low intra-
cellular Cl-concentration, which is required for the functioning of Cl- 
dependent fast synaptic inhibition, mediated by certain neurotransmitters,
such as gamma-aminobutyric acid (GABA) and glycine.
 
Two isoforms of the K-Cl co-transporter have been described, termed KCC1 and
KCC2, containing 1085 and 1116 amino acids, respectively. They are both 
predicted to have 12 transmembrane (TM) regions in a central hydrophobic 
domain, together with hydrophilic N- and C-termini that are likely
cytoplasmic. Comparison of their sequences with those of other ion-
tranporting membrane proteins reveals that they are part of a new super-
family of cation-chloride co-transporters, which includes the Na-Cl and 
Na-K-2Cl co-transporters. KCC1 is widely expressed in human tissues, while
KCC2 is expressed only in brain neurones, making it likely that this is the
isoform responsible for maintaining low Cl- concentration in neurones [2,3].
 
KCLTRNSPORT is a 6-element fingerprint that provides a signature for K-Cl
co-transporters. The fingerprint was derived from an initial alignment of 8
sequences: the motifs were drawn from conserved regions within the
N-terminal half of the alignment - motifs 1 and 2 reside within the
putative cytoplasmic N-terminus; and motifs 3-6 lie within the central
hydrophobic domain. A single iteration on OWL31.1 was required to reach
convergence, no further sequences being identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 7 sequences.
Summary Information
7 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6777777
5000000
4000000
3000000
2000000
123456
True Positives
O18887        O55069        O60632        Q13953        
Q28677 Q63632 Q63633
Sequence Titles
O18887      K-CL COTRANSPORTER - SUS SCROFA (PIG).        
O55069 ERYTHROID K:CL COTRANSPORTER - MUS MUSCULUS (MOUSE).
O60632 ERYTHROID K:CL COTRANSPORTER SPLICING ISOFORM 2 - HOMO SAPIENS (HUMAN).
Q13953 K-CL COTRANSPORTER - HOMO SAPIENS (HUMAN).
Q28677 K-CL COTRANSPORTER - ORYCTOLAGUS CUNICULUS (RABBIT).
Q63632 FUROSEMIDE-SENSITIVE K-CL COTRANSPORTER - RATTUS NORVEGICUS (RAT).
Q63633 FUROSEMIDE-SENSITIVE K-CL COTRANSPORTER - RATTUS NORVEGICUS (RAT).
Scan History
OWL31_1    1  100  NSINGLE    
SPTR37_9f 2 8 NSINGLE
Initial Motifs
Motif 1  width=7
Element Seqn Id St Int Rpt
KESSPFL AF028807 44 44 -
RESSPFL OCKCC 44 44 -
RESSPFL AF047338 44 44 -
RESSPFL AF054506 44 44 -
RESSPFL AF054505 44 44 -
RENSPFL AF047339 44 44 -
RENSPFL RNU55815 44 44 -
KESSPFI RNU55816 23 23 -

Motif 2 width=9
Element Seqn Id St Int Rpt
RNLALFEEE AF028807 64 13 -
RNLALFEEE OCKCC 64 13 -
RNLALFEEE AF047338 64 13 -
RNLALFEEE AF054506 64 13 -
RNLALFEEE AF054505 64 13 -
RNLALFEEE AF047339 64 13 -
RNLALFEEE RNU55815 64 13 -
RNMALFEEE RNU55816 43 13 -

Motif 3 width=13
Element Seqn Id St Int Rpt
VVFVGVKYVNKFA AF028807 265 192 -
VVFVGVKYVNKFA OCKCC 265 192 -
VVFVGVKYVNKFA AF047338 265 192 -
VVFVGVKYVNKFA AF054506 265 192 -
VVFVGVKYVNKFA AF054505 265 192 -
VVFVGVKYVNKFA AF047339 265 192 -
VVFVGVKYVNKFA RNU55815 265 192 -
VVFVGVKYVNKFA RNU55816 244 192 -

Motif 4 width=18
Element Seqn Id St Int Rpt
FDPPVFPVCMLGNRTLSR AF028807 300 22 -
FDPPVFPVCMLGNRTLSR OCKCC 300 22 -
FDPPVFPVCMLGNRTLSR AF047338 300 22 -
FDPPVFPVCMLGNRTLSR AF054506 300 22 -
FDPPVFPVCMLGNRTLSR AF054505 300 22 -
FDPPVFPVCMLGNRTLSR AF047339 300 22 -
FDPPVFPVCMLGNRTLSR RNU55815 300 22 -
FDPPNFPICLLGNRTLSR RNU55816 279 22 -

Motif 5 width=17
Element Seqn Id St Int Rpt
PFLRVFGHGKANGEPTW AF028807 540 222 -
PFLRVFGHGKANGEPTW OCKCC 540 222 -
PFLRVFGHGKVNGEPTW AF047338 540 222 -
PFLRVFGHGKVNGEPTW AF054506 540 222 -
PFLRVFGHGKVNGEPTW AF054505 540 222 -
PFLRVFGHGKANGEPTW AF047339 540 222 -
PFLRVFGHGKANGEPTW RNU55815 540 222 -
PFLQVFGHGKANGEPTW RNU55816 520 223 -

Motif 6 width=9
Element Seqn Id St Int Rpt
ELGILIASL AF028807 566 9 -
ELGILIASL OCKCC 566 9 -
ELGILIASL AF047338 566 9 -
ELGILIASL AF054506 566 9 -
ELGILIASL AF054505 566 9 -
ELGILIASL AF047339 566 9 -
ELGILIASL RNU55815 566 9 -
EIGILIASL RNU55816 546 9 -
Final Motifs
Motif 1  width=7
Element Seqn Id St Int Rpt
RESSPFL Q28677 44 44 -
RESSPFL O60632 44 44 -
RESSPFL Q13953 44 44 -
KESSPFL O18887 44 44 -
RENSPFL O55069 44 44 -
RENSPFL Q63632 44 44 -
KESSPFI Q63633 23 23 -

Motif 2 width=9
Element Seqn Id St Int Rpt
RNLALFEEE Q28677 64 13 -
RNLALFEEE O60632 64 13 -
RNLALFEEE Q13953 64 13 -
RNLALFEEE O18887 64 13 -
RNLALFEEE O55069 64 13 -
RNLALFEEE Q63632 64 13 -
RNMALFEEE Q63633 43 13 -

Motif 3 width=13
Element Seqn Id St Int Rpt
VVFVGVKYVNKFA Q28677 265 192 -
VVFVGVKYVNKFA O60632 265 192 -
VVFVGVKYVNKFA Q13953 265 192 -
VVFVGVKYVNKFA O18887 265 192 -
VVFVGVKYVNKFA O55069 265 192 -
VVFVGVKYVNKFA Q63632 265 192 -
VVFVGVKYVNKFA Q63633 244 192 -

Motif 4 width=18
Element Seqn Id St Int Rpt
FDPPVFPVCMLGNRTLSR Q28677 300 22 -
FDPPVFPVCMLGNRTLSR O60632 300 22 -
FDPPVFPVCMLGNRTLSR Q13953 300 22 -
FDPPVFPVCMLGNRTLSR O18887 300 22 -
FDPPVFPVCMLGNRTLSR O55069 300 22 -
FDPPVFPVCMLGNRTLSR Q63632 300 22 -
FDPPNFPICLLGNRTLSR Q63633 279 22 -

Motif 5 width=17
Element Seqn Id St Int Rpt
PFLRVFGHGKANGEPTW Q28677 540 222 -
PFLRVFGHGKVNGEPTW O60632 540 222 -
PFLRVFGHGKVNGEPTW Q13953 540 222 -
PFLRVFGHGKANGEPTW O18887 540 222 -
PFLRVFGHGKANGEPTW O55069 540 222 -
PFLRVFGHGKANGEPTW Q63632 540 222 -
PFLQVFGHGKANGEPTW Q63633 520 223 -

Motif 6 width=9
Element Seqn Id St Int Rpt
ELGILIASL Q28677 566 9 -
ELGILIASL O60632 566 9 -
ELGILIASL Q13953 566 9 -
ELGILIASL O18887 566 9 -
ELGILIASL O55069 566 9 -
ELGILIASL Q63632 566 9 -
EIGILIASL Q63633 546 9 -