SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01056

Identifier
MTABOTROPC6R  [View Relations]  [View Alignment]  
Accession
PR01056
No. of Motifs
7
Creation Date
30-JAN-1999  (UPDATE 07-JUN-1999)
Title
Metabotropic glutamate receptor 6 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00248 GPCRMGR
PRINTS; PR00593 MTABOTROPICR
INTERPRO; IPR000112
GCRDB; GCR_0623; GCR_2607
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994). 
 
2. MASU, M., TANABE, Y., TSUCHIDA, K., SHIGEMOTO, R. AND NAKANISHI, S.
Sequence and expression of a metabotropic glutamate receptor.
NATURE 349 760-765 (1991).
 
3. HOUAMED, K.M., KUIJPER, J.L., GILBERT, T.L., HALDEMAN, B.A., OHARA, P.J.,
MULVIHILL, E.R., ALMERS, W. AND HAGEN, F.S.
Cloning, expression and gene structure of a G protein-coupled glutamate
receptor from rat brain.
SCIENCE 252 1318-1321 (1991).
 
4. ABE, T., SUGIHARA, H., NAWA, H., SHIGEMOTO, R., MIZUNO, N. AND 
NAKANISHI, S.
Molecular characterisation of a novel metabotropic glutamate receptor
MGLUR5 coupled to inositol phosphate/Ca2+ signal transduction.
J.BIOL.CHEM. 267(19) 13361-13368 (1992).
 
5. TANABE, Y., MASU, M., ISHII, T., SHIGEMOTO, R. AND NAKANISHI, S.
A family of metabotropic glutamate receptors.
NEURON 8(1) 169-179 (1992).
 
6. WATSON, S. AND ARKINSTALL, S.
Glutamate receptors.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.130-141.
 
7. HASHIMOTO, T., INAZAWA, J., OKAMOTO, N., TAGAWA, Y., BESSHO, Y.,
HONDA, Y. AND NAKANISHI, S.
The whole nucleotide sequence and chromosomal localization of the gene for
human metabotropic glutamate receptor subtype 6.
EUR.J.NEUROSCIENCE 9 1226-1235 (1997). 
 
8. NAKAJIMA, Y., IWAKABE, H., AKAZAWA, C., NAWA, H., SHIGEMOTO, R.,
MIZUNO, N. AND NAKANISHI, S.
Molecular characterization of a novel retinal metabotropic glutamate
receptor mGluR6 with a high agonist selectivity for L-2-amino-4-
phosphonobutyrate.
J.BIOL.CHEM. 268 11868-11873 (1993). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The metabotropic glutamate receptors are functionally and pharmacologically
distinct from the ionotropic glutamate receptors. They are coupled to G-
proteins and stimulate the inositol phophate/Ca2+ intracellular signalling 
pathway [2-5]. The amino acid sequences of the receptors contain 2 domains:
a large extracellular domain, and a membrane-associated domain that contains
high proportions of hydrophobic residues grouped into 7 regions, in a manner
reminiscent of the rhodopsins and other receptors believed to interact with
G proteins. However, while a similar 3D framework has been proposed to
account for this, there is no significant sequence identity between these
and receptors of the rhodopsin-type family: the metabotropic glutamate
receptors thus bear their own distinctive `7TM' signature. This 7TM
signature is also shared by the calcium-sensing receptors.
 
At least eight sub-types of metabotropic receptor (MGR1-8) have been 
identified in cloning studies. The sub-types differ in their agonist 
pharmacology and signal transduction pathways [6]. MGR6 is restrictedly
expressed in retinal ON bipolar cells and, on the basis of its agonist
selectivity, may have a key physiological role in certain inherited eye
diseases [7,8].
 
MTABOTROPC6R is a 7-element fingerprint that provides a signature for the
metabotropic glutamate receptor 6 precursor. The fingerprint was derived
from an initial alignment of 2 sequences: the motifs were drawn from short
conserved regions spanning the full alignment length, focusing on those
sections that characterise the metabotropic glutamate type 6 receptors but 
distinguish them from the rest of the metabotropic glutamate receptor family
- motifs 1-6 reside in the N-terminal extracellular domain; and motif 7 lies
in the C-terminal cytoplasmic domain. A single iteration on OWL31.1 was
required to reach convergence, no further sequences being identified beyond 
the starting set.
 
An update on SPTR37_9f identified a true set of 2 sequences.
Summary Information
2 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
72222222
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
MGR6_HUMAN    MGR6_RAT      
Sequence Titles
MGR6_HUMAN  METABOTROPIC GLUTAMATE RECEPTOR 6 PRECURSOR - HOMO SAPIENS (HUMAN). 
MGR6_RAT METABOTROPIC GLUTAMATE RECEPTOR 6 PRECURSOR - RATTUS NORVEGICUS (RAT).
Scan History
OWL31_1    1  100  NSINGLE    
SPTR37_9f 2 3 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
WLAQAGLARAAGSVRLAG MGR6_HUMAN 20 20 -
WLSQAGIACGAGSVRLAG MGR6_RAT 14 14 -

Motif 2 width=17
Element Seqn Id St Int Rpt
RGRGDGDEVGVRCPGGV MGR6_HUMAN 118 80 -
RGRGDGDEASVRCPGGV MGR6_RAT 112 80 -

Motif 3 width=16
Element Seqn Id St Int Rpt
VPPLRPAPPERVVAVV MGR6_HUMAN 134 -1 -
VPPLRSAPPERVVAVV MGR6_RAT 128 -1 -

Motif 4 width=13
Element Seqn Id St Int Rpt
LTSSGTQSDDSTR MGR6_HUMAN 369 219 -
LTSSGGQSDDSTR MGR6_RAT 363 219 -

Motif 5 width=14
Element Seqn Id St Int Rpt
ATNGSASSGGYQAV MGR6_HUMAN 477 95 -
ATNGSASSGGYQAV MGR6_RAT 471 95 -

Motif 6 width=18
Element Seqn Id St Int Rpt
WAETLRLDVEALQWSGDP MGR6_HUMAN 493 2 -
WAEALRLDMEVLRWSGDP MGR6_RAT 487 2 -

Motif 7 width=18
Element Seqn Id St Int Rpt
SLKATSTVAAPPKGEDAE MGR6_HUMAN 857 346 -
SLKKTSTMAAPPQNENAE MGR6_RAT 851 346 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
WLAQAGLARAAGSVRLAG MGR6_HUMAN 20 20 -
WLSQAGIACGAGSVRLAG MGR6_RAT 14 14 -

Motif 2 width=17
Element Seqn Id St Int Rpt
RGRGDGDEVGVRCPGGV MGR6_HUMAN 118 80 -
RGRGDGDEASVRCPGGV MGR6_RAT 112 80 -

Motif 3 width=16
Element Seqn Id St Int Rpt
VPPLRPAPPERVVAVV MGR6_HUMAN 134 -1 -
VPPLRSAPPERVVAVV MGR6_RAT 128 -1 -

Motif 4 width=13
Element Seqn Id St Int Rpt
LTSSGTQSDDSTR MGR6_HUMAN 369 219 -
LTSSGGQSDDSTR MGR6_RAT 363 219 -

Motif 5 width=14
Element Seqn Id St Int Rpt
ATNGSASSGGYQAV MGR6_HUMAN 477 95 -
ATNGSASSGGYQAV MGR6_RAT 471 95 -

Motif 6 width=18
Element Seqn Id St Int Rpt
WAETLRLDVEALQWSGDP MGR6_HUMAN 493 2 -
WAEALRLDMEVLRWSGDP MGR6_RAT 487 2 -

Motif 7 width=18
Element Seqn Id St Int Rpt
SLKATSTVAAPPKGEDAE MGR6_HUMAN 857 346 -
SLKKTSTMAAPPQNENAE MGR6_RAT 851 346 -