SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01051

Identifier
MTABOTROPC1R  [View Relations]  [View Alignment]  
Accession
PR01051
No. of Motifs
9
Creation Date
28-JAN-1999  (UPDATE 03-JUL-1999)
Title
Metabotropic glutamate receptor 1 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00248 GPCRMGR
PRINTS; PR00593 MTABOTROPICR
INTERPRO; IPR001256
GCRDB; GCR_1825; GCR_1826; GCR_1982; GCR_1983; GCR_0216
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994). 
 
2. MASU, M., TANABE, Y., TSUCHIDA, K., SHIGEMOTO, R. AND NAKANISHI, S.
Sequence and expression of a metabotropic glutamate receptor.
NATURE 349 760-765 (1991).
 
3. HOUAMED, K.M., KUIJPER, J.L., GILBERT, T.L., HALDEMAN, B.A., OHARA, P.J.,
MULVIHILL, E.R., ALMERS, W. AND HAGEN, F.S.
Cloning, expression and gene structure of a G protein-coupled glutamate
receptor from rat brain.
SCIENCE 252 1318-1321 (1991).
 
4. ABE, T., SUGIHARA, H., NAWA, H., SHIGEMOTO, R., MIZUNO, N. AND 
NAKANISHI, S.
Molecular characterisation of a novel metabotropic glutamate receptor
MGLUR5 coupled to inositol phosphate/Ca2+ signal transduction.
J.BIOL.CHEM. 267(19) 13361-13368 (1992).
 
5. TANABE, Y., MASU, M., ISHII, T., SHIGEMOTO, R. AND NAKANISHI, S.
A family of metabotropic glutamate receptors.
NEURON 8(1) 169-179 (1992).
 
6. WATSON, S. AND ARKINSTALL, S.
Glutamate receptors.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.130-141.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The metabotropic glutamate receptors are functionally and pharmacologically
distinct from the ionotropic glutamate receptors. They are coupled to G-
proteins and stimulate the inositol phophate/Ca2+ intracellular signalling 
pathway [2-5]. The amino acid sequences of the receptors contain 2 domains:
a large extracellular domain, and a membrane-associated domain that contains
high proportions of hydrophobic residues grouped into 7 regions, in a manner
reminiscent of the rhodopsins and other receptors believed to interact with
G proteins. However, while a similar 3D framework has been proposed to
account for this, there is no significant sequence identity between these
and receptors of the rhodopsin-type family: the metabotropic glutamate
receptors thus bear their own distinctive `7TM' signature. This 7TM
signature is also shared by the calcium-sensing receptors.
 
At least eight sub-types of metabotropic receptor (MGR1-8) have been 
identified in cloning studies. The sub-types differ in their agonist 
pharmacology and signal transduction pathways [6]. mRNA for MGR1 is
widespread in the brain and is abundant in neuronal cells in hippocampal
dentate gyrus and CA2-3 regions, cerebellum Purkinje cells, olfactory bulb
and thalamic nuclei [6]. MGR1 activates the phophoinositide pathway,
probably via a G protein of the Gq/G11 class; pertussis toxin partially
inhibits the response to MGR1, but not to MGR5 [6].
 
MTABOTROPC1R is a 9-element fingerprint that provides a signature for the
metabotropic glutamate receptor 1 precursor. The fingerprint was derived
from an initial alignment of 2 sequences: the motifs were drawn from short
conserved regions spanning the full alignment length, focusing on those
sections that characterise the metabotropic glutamate type 1 receptors but 
distinguish them from the rest of the metabotropic glutamate receptor family
- motifs 1-5 reside in the N-terminal extracellular domain; and motifs 6-9 
lie in the C-terminal cytoplasmic domain. A single iteration on OWL31.1 was 
required to reach convergence, no further sequences being identified beyond
the starting set. Several partial matches were found: HSU31216, HUMMGLUB 
and S71376 are type 1 receptor homologues that fail to match 3 motifs; and 
JC2132, MGR5_HUMAN and MGR5_RAT are type 5 receptors that match two motifs.
 
An update on SPTR37_9f identified a true set of 2 sequences, and 2
partial matches.
Summary Information
   2 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
9222222222
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000022
123456789
True Positives
MGR1_HUMAN    MGR1_RAT      
True Positive Partials
Codes involving 2 elements
MGR5_HUMAN MGR5_RAT
Sequence Titles
MGR1_HUMAN  METABOTROPIC GLUTAMATE RECEPTOR 1 PRECURSOR - HOMO SAPIENS (HUMAN). 
MGR1_RAT METABOTROPIC GLUTAMATE RECEPTOR 1 PRECURSOR - RATTUS NORVEGICUS (RAT).

MGR5_HUMAN METABOTROPIC GLUTAMATE RECEPTOR 5 PRECURSOR - HOMO SAPIENS (HUMAN).
MGR5_RAT METABOTROPIC GLUTAMATE RECEPTOR 5 PRECURSOR - RATTUS NORVEGICUS (RAT).
Scan History
OWL31_1    1  50   NSINGLE    
SPTR37_9f 2 50 NSINGLE
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
LLLFFFPAIFLEVSLLPRSPGRK MGR1_HUMAN 4 4 -
LLLIFFPMIFLEMSILPRMPDRK MGR1_RAT 4 4 -

Motif 2 width=15
Element Seqn Id St Int Rpt
KVLLAGASSQRSVAR MGR1_HUMAN 26 -1 -
KVLLAGASSQRSVAR MGR1_RAT 26 -1 -

Motif 3 width=22
Element Seqn Id St Int Rpt
SIRDEKDGINRCLPDGQSLPPG MGR1_HUMAN 129 88 -
SIRDEKDGLNRCLPDGQTLPPG MGR1_RAT 129 88 -

Motif 4 width=24
Element Seqn Id St Int Rpt
LPGHLLENPNFKRICTGNESLEEN MGR1_HUMAN 380 229 -
LPGHLLENPNFKKVCTGNESLEEN MGR1_RAT 380 229 -

Motif 5 width=20
Element Seqn Id St Int Rpt
LQYTEANRYDYVHVGTWHEG MGR1_HUMAN 484 80 -
LQYTEANRYDYVHVGTWHEG MGR1_RAT 484 80 -

Motif 6 width=22
Element Seqn Id St Int Rpt
KLPCRSNTFLNIFRRKKAGAGN MGR1_HUMAN 864 360 -
KLPCRSNTFLNIFRRKKPGAGN MGR1_RAT 864 360 -

Motif 7 width=22
Element Seqn Id St Int Rpt
TKSYQGSGKSLTFSDTSTKTLY MGR1_HUMAN 934 48 -
TKSYQGSGKSLTFSDASTKTLY MGR1_RAT 934 48 -

Motif 8 width=20
Element Seqn Id St Int Rpt
SKLTPDDSPALTPPSPFRDS MGR1_HUMAN 1135 179 -
SKLTPEDSPALTPPSPFRDS MGR1_RAT 1140 184 -

Motif 9 width=23
Element Seqn Id St Int Rpt
SESVLCTPPNVSYASVILRDYKQ MGR1_HUMAN 1167 12 -
SESVLCTPPNVTYASVILRDYKQ MGR1_RAT 1172 12 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
LLLFFFPAIFLEVSLLPRSPGRK MGR1_HUMAN 4 4 -
LLLIFFPMIFLEMSILPRMPDRK MGR1_RAT 4 4 -

Motif 2 width=15
Element Seqn Id St Int Rpt
KVLLAGASSQRSVAR MGR1_HUMAN 26 -1 -
KVLLAGASSQRSVAR MGR1_RAT 26 -1 -

Motif 3 width=22
Element Seqn Id St Int Rpt
SIRDEKDGINRCLPDGQSLPPG MGR1_HUMAN 129 88 -
SIRDEKDGLNRCLPDGQTLPPG MGR1_RAT 129 88 -

Motif 4 width=24
Element Seqn Id St Int Rpt
LPGHLLENPNFKRICTGNESLEEN MGR1_HUMAN 380 229 -
LPGHLLENPNFKKVCTGNESLEEN MGR1_RAT 380 229 -

Motif 5 width=20
Element Seqn Id St Int Rpt
LQYTEANRYDYVHVGTWHEG MGR1_HUMAN 484 80 -
LQYTEANRYDYVHVGTWHEG MGR1_RAT 484 80 -

Motif 6 width=22
Element Seqn Id St Int Rpt
KLPCRSNTFLNIFRRKKAGAGN MGR1_HUMAN 864 360 -
KLPCRSNTFLNIFRRKKPGAGN MGR1_RAT 864 360 -

Motif 7 width=22
Element Seqn Id St Int Rpt
TKSYQGSGKSLTFSDTSTKTLY MGR1_HUMAN 934 48 -
TKSYQGSGKSLTFSDASTKTLY MGR1_RAT 934 48 -

Motif 8 width=20
Element Seqn Id St Int Rpt
SKLTPDDSPALTPPSPFRDS MGR1_HUMAN 1135 179 -
SKLTPEDSPALTPPSPFRDS MGR1_RAT 1140 184 -

Motif 9 width=23
Element Seqn Id St Int Rpt
SESVLCTPPNVSYASVILRDYKQ MGR1_HUMAN 1167 12 -
SESVLCTPPNVTYASVILRDYKQ MGR1_RAT 1172 12 -