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PR01031

Identifier
ORPHNNPRCRSR  [View Relations]  [View Alignment]  
Accession
PR01031
No. of Motifs
8
Creation Date
01-DEC-1998  (UPDATE 07-JUN-1999)
Title
Nociceptin precursor signature
Database References
PRINTS; PR01028 OPIOIDPRCRSR
INTERPRO; IPR002367
Literature References
1. MOLLEREAU, C., SIMONS, M.-J., SOULARUE, P., LINERS, F., VASSART, G.,
MEUNIER, J.-C. AND PARMENTIER, M.
Structure, tissue distribution, and chromosomal localization of the
prepronociceptin gene.
PROC.NATL.ACAD.SCI.U.S.A. 93 8666-8670 (1996).
 
2. SAITO, Y., MARUYAMA, K., SAIDO, T.C. AND KAWASHIMA, S.
N23K, a gene transiently up-regulated during neural differentiation,
encodes a precursor protein for a newly identified neuropeptide nociceptin.
BIOCHEM.BIOPHYS.RES.COMMUN. 217 539-545 (1995). 

Documentation
Vertebrate endogenous opioid neuropeptides are released by posttransla-
tional proteolytic cleavage of precursor proteins. The precursors consist of
the following components: a signal sequence that precedes a conserved region
of about 50 residues; a variable-length region; and the sequence of the 
neuropeptide itself. Three types of precursor are known: preproenkephalin A
(gene PENK), which is processed to produce 6 copies of Met-enkephalin, plus
Leu-enkephalin; preproenkephalin B (gene PDYN), which is processed to
produce neoendorphin, dynorphin, leumorphin, rimorphin and Leu-enkephalin;
and prepronocipeptin (gene PNOC), whose processing produces nociceptin
(orphanin FQ) and two other potential neuropeptides.
 
Sequence analysis reveals that the conserved N-terminal region of the
precursors contains 6 cysteines, which are probably involved in disulphide
bond formation. It is speculated that this region might be important for 
neuropeptide processing [1].
 
Nociceptin (or orphanin FQ) is a natural agonist of opioid receptor-like
receptor [1]. The peptide is derived from the prepronociceptin precursor,
whose gene is predominantly transcribed in the central nervous system 
(brain and spinal cord) and, weakly, in the ovary (the sole peripheral
organ that expresses the gene) [1]. Studies on rat and mouse nociceptin
suggest that the protein functions not only as a neuropeptide precursor
but also as an important component in neuronal differentiation [2]. 
 
ORPHNNPRCRSR is an 8-element fingerprint that provides a signature for the
nociceptin precursor. The fingerprint was derived from an initial alignment 
of 5 sequences: the motifs were drawn from short conserved regions spanning 
the full alignment length, focusing on those regions that characterise the
nociceptin precursors but distinguish them from the rest of the opioid 
neuropeptide precursor family - motifs 1-4 span the N-terminal region 
encoded by PROSITE pattern OPIOIDS_PRECURSOR (PS01252), which contains the 
six conserved cysteines thought to be involved in disulphide bond formation;
motifs 5 and 6 span a conserved region thought to encode a neuropeptide; 
the nociceptin peptide is encoded within the C-terminal portion of motif 6
and the N-terminal half of motif 7; and a second possible neuropeptide 
spans motifs 7 and 8. A single iteration on OWL30.2 was required to reach 
convergence, no further sequences being identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 4 sequences.
Summary Information
4 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
844444444
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
PNOC_BOVIN    PNOC_HUMAN    PNOC_MOUSE    PNOC_RAT      
Sequence Titles
PNOC_BOVIN  NOCICEPTIN PRECURSOR (ORPHANIN FQ) (PPNOC) - BOS TAURUS (BOVINE). 
PNOC_HUMAN NOCICEPTIN PRECURSOR (ORPHANIN FQ) (PPNOC) - HOMO SAPIENS (HUMAN).
PNOC_MOUSE NOCICEPTIN PRECURSOR (ORPHANIN FQ) (PPNOC) (N23K/N27K) - MUS MUSCULUS (MOUSE).
PNOC_RAT NOCICEPTIN PRECURSOR (ORPHANIN FQ) (PPNOC) (ORL1 RECEPTOR AGONIST) - RATTUS NORVEGICUS (RAT).
Scan History
OWL30_2    1  100  NSINGLE    
SPTR37_9f 2 5 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
KILFCDVLLLSLLSSVFSSC PNOC_RAT 2 2 -
KVLLCDLLLLSLFSSVFSSC PNOC_HUMAN 2 2 -
KILFCDVLLLSLLSSVFSSC PNOC_MOUSE 2 2 -
KILFCDLLLLSLFSSVSSSC AB005251 2 2 -
KILFCDVLLLSLLSSVFSSC MUSN27K 2 2 -

Motif 2 width=16
Element Seqn Id St Int Rpt
DCLTCQEKLHPALDSF PNOC_HUMAN 24 2 -
DCLTCQEKLHPAPDSF MUSN27K 24 2 -
DCLVCREKLRPTLDSF AB005251 24 2 -
DCLTCQEKLHPAPDSF PNOC_MOUSE 24 2 -
DCLTCQERLHPAPGSF PNOC_RAT 24 2 -

Motif 3 width=19
Element Seqn Id St Int Rpt
LEMCILECEEKAFTSPLWT AB005251 41 1 -
LKLCILQCEEKVFPRPLWT PNOC_RAT 41 1 -
LEVCILECEEKVFPSPLWT PNOC_HUMAN 41 1 -
LKTCILQCEEKVFPRPLWT PNOC_MOUSE 41 1 -
LKTCILQCEEKVFPRPLWT MUSN27K 41 1 -

Motif 4 width=18
Element Seqn Id St Int Rpt
CTKVMARGSWQLSPADPD AB005251 61 1 -
CTKAMASDSEQLSPADPE PNOC_RAT 61 1 -
CTKVMARSSWQLSPAAPE PNOC_HUMAN 61 1 -
CTKVMASGSGQLSPADPE PNOC_MOUSE 61 1 -
CTKVMASGSGQLSPADPE MUSN27K 61 1 -

Motif 5 width=22
Element Seqn Id St Int Rpt
AALDQPRASEMQHLKRMPRVRS AB005251 82 3 -
AALYQPKASEMQHLKRMPRVRS PNOC_MOUSE 82 3 -
AALYQPRASEMQHLRRMPRVRS PNOC_HUMAN 82 3 -
AALYQSKASEMQHLKRMPRVRS PNOC_RAT 82 3 -
AALYQPKASEMQHLKRMPRVRS MUSN27K 82 3 -

Motif 6 width=22
Element Seqn Id St Int Rpt
MEEAGEMEQKQLQKRFGGFTGA PNOC_HUMAN 115 11 -
ADDAEEVEQKQLQKRFGGFTGA PNOC_MOUSE 126 22 -
ADDAEEVEQKQLQKRFGGFTGA MUSN27K 126 22 -
LEEVGEIEQKQLQKRFGGFTGA AB005251 115 11 -
ADEADEVEQKQLQKRFGGFTGA PNOC_RAT 120 16 -

Motif 7 width=19
Element Seqn Id St Int Rpt
ARKSARKLANQKRFSEFMR AB005251 136 -1 -
ARKSARKLANQKRFSEFMR PNOC_RAT 141 -1 -
ARKSARKLANQKRFSEFMR PNOC_MOUSE 147 -1 -
ARKSARKLANQKRFSEFMR PNOC_HUMAN 136 -1 -
ARKSARKLANQKRFSEFMR MUSN27K 147 -1 -

Motif 8 width=21
Element Seqn Id St Int Rpt
QYLVLSMQSSQRRRTLHQNGI MUSN27K 166 0 -
QYLVLSMQSSQRRRTLHQNGN PNOC_RAT 160 0 -
QYLVLSMQSSQRRRTLHQNGN PNOC_HUMAN 155 0 -
QYLVLSMQSSQRRRTLHQNGN PNOC_MOUSE 166 0 -
QYLVLSMQSSQRRRTLHQNGN AB005251 155 0 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
KILFCDLLLLSLFSSVSSSC PNOC_BOVIN 2 2 -
KILFCDVLLLSLLSSVFSSC PNOC_MOUSE 2 2 -
KVLLCDLLLLSLFSSVFSSC PNOC_HUMAN 2 2 -
KILFCDVLLLSLLSSVFSSC PNOC_RAT 2 2 -

Motif 2 width=16
Element Seqn Id St Int Rpt
DCLVCREKLRPTLDSF PNOC_BOVIN 24 2 -
DCLTCQEKLHPALDSF PNOC_HUMAN 24 2 -
DCLTCQERLHPAPGSF PNOC_RAT 24 2 -
DCLTCQEKLHPAPDSF PNOC_MOUSE 24 2 -

Motif 3 width=19
Element Seqn Id St Int Rpt
LKTCILQCEEKVFPRPLWT PNOC_MOUSE 41 1 -
LEVCILECEEKVFPSPLWT PNOC_HUMAN 41 1 -
LKLCILQCEEKVFPRPLWT PNOC_RAT 41 1 -
LEMCILECEEKAFTSPLWT PNOC_BOVIN 41 1 -

Motif 4 width=18
Element Seqn Id St Int Rpt
CTKVMASGSGQLSPADPE PNOC_MOUSE 61 1 -
CTKVMARSSWQLSPAAPE PNOC_HUMAN 61 1 -
CTKAMASDSEQLSPADPE PNOC_RAT 61 1 -
CTKVMARGSWQLSPADPD PNOC_BOVIN 61 1 -

Motif 5 width=22
Element Seqn Id St Int Rpt
AALYQPKASEMQHLKRMPRVRS PNOC_MOUSE 82 3 -
AALYQPRASEMQHLRRMPRVRS PNOC_HUMAN 82 3 -
AALYQSKASEMQHLKRMPRVRS PNOC_RAT 82 3 -
AALDQPRASEMQHLKRMPRVRS PNOC_BOVIN 82 3 -

Motif 6 width=22
Element Seqn Id St Int Rpt
ADDAEEVEQKQLQKRFGGFTGA PNOC_MOUSE 126 22 -
MEEAGEMEQKQLQKRFGGFTGA PNOC_HUMAN 115 11 -
ADEADEVEQKQLQKRFGGFTGA PNOC_RAT 120 16 -
LEEVGEIEQKQLQKRFGGFTGA PNOC_BOVIN 115 11 -

Motif 7 width=19
Element Seqn Id St Int Rpt
ARKSARKLANQKRFSEFMR PNOC_MOUSE 147 -1 -
ARKSARKLANQKRFSEFMR PNOC_HUMAN 136 -1 -
ARKSARKLANQKRFSEFMR PNOC_RAT 141 -1 -
ARKSARKLANQKRFSEFMR PNOC_BOVIN 136 -1 -

Motif 8 width=21
Element Seqn Id St Int Rpt
QYLVLSMQSSQRRRTLHQNGN PNOC_MOUSE 166 0 -
QYLVLSMQSSQRRRTLHQNGN PNOC_HUMAN 155 0 -
QYLVLSMQSSQRRRTLHQNGN PNOC_RAT 160 0 -
QYLVLSMQSSQRRRTLHQNGN PNOC_BOVIN 155 0 -