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PR01030

Identifier
PENKBPRCRSR  [View Relations]  [View Alignment]  
Accession
PR01030
No. of Motifs
7
Creation Date
02-DEC-1998  (UPDATE 07-JUN-1999)
Title
Proenkephalin B precursor signature
Database References
PRINTS; PR01028 OPIOIDPRCRSR
INTERPRO; IPR000750
Literature References
1. MOLLEREAU, C., SIMONS, M.-J., SOULARUE, P., LINERS, F., VASSART, G.,
MEUNIER, J.-C. AND PARMENTIER, M.
Structure, tissue distribution, and chromosomal localization of the
prepronociceptin gene.
PROC.NATL.ACAD.SCI.U.S.A. 93 8666-8670 (1996).
 
2. SAITO, Y., MARUYAMA, K., SAIDO, T.C. AND KAWASHIMA, S.
N23K, a gene transiently up-regulated during neural differentiation,
encodes a precursor protein for a newly identified neuropeptide nociceptin.
BIOCHEM.BIOPHYS.RES.COMMUN. 217 539-545 (1995). 
 
3. HORIKAWA, S., TAKAI, T., TOYOSATO, M., TAKAHASHI, H., NODA, M.,
KAKIDANI, H., KUBO, T., HIROSE, T., INAYAMA, S. AND HAYASHIDA, H.
Isolation and structural organization of the human preproenkephalin B gene.
NATURE 306 611-614 (1983).

Documentation
Vertebrate endogenous opioid neuropeptides are released by post-transla-
tional proteolytic cleavage of precursor proteins. The precursors consist of
the following components: a signal sequence that precedes a conserved region
of about 50 residues; a variable-length region; and the sequence of the
neuropeptide itself. Three types of precursor are known: preproenkephalin A
(gene PENK), which is processed to produce 6 copies of Met-enkephalin, plus
Leu-enkephalin; preproenkephalin B (gene PDYN), which is processed to
produce neoendorphin, dynorphin, leumorphin, rimorphin and Leu-enkephalin;
and prepronocipeptin (gene PNOC), whose processing produces nociceptin
(orphanin FQ) and two other potential neuropeptides.
 
Sequence analysis reveals that the conserved N-terminal region of the
precursors contains 6 cysteines, which are probably involved in disulphide
bond formation. It is speculated that this region might be important for 
neuropeptide processing [1].
 
The primary structure of porcine preproenkephalin B has been elucidated and
shown to contain neoendorphin, dynorphin and leumorphin (containing 
rimorphin as its N-terminus). These opioid peptides, each having a Leu-
enkephalin structure, act on the kappa-receptor. The sequence similarity
observed between preproenkephalin B and preproenkephalin A, coupled with the
similarity between their gene organisations, suggests that the two genes
have been generated from a common ancestor by gene duplication [3]. 
 
PENKBPRCRSR is a 7-element fingerprint that provides a signature for the
proenkephalin B precursor. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from short conserved regions 
spanning the full alignment length, focusing on those regions that 
characterise the proenkephalin B precursors but distinguish them from the
rest of the opioid neuropeptide precursor family - motifs 1 lies in the
signal sequence; motifs 2 and 3 reside within the N-terminal Cys-rich region
encoded by PROSITE pattern OPIOIDS_PRECURSOR (PS01252), falling between the 
third and fourth, and fifth and sixth conserved Cys residues respectively;
motifs 4 and 5 lie within the variable region; and motifs 6 and 7 span the
dynorphin and leumorphin peptides respectively. Two iterations on OWL30.2
were required to reach convergence, at which point a true set comprising 7
sequences was identified.
 
An update on SPTR37_9f identified a true set of 6 sequences.
Summary Information
6 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
76666666
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
NDDB_BOVIN    NDDB_CAVPO    NDDB_HUMAN    NDDB_PIG      
NDDB_RAT O35417
Sequence Titles
NDDB_BOVIN  BETA-NEOENDORPHIN-DYNORPHIN PRECURSOR (PROENKEPHALIN B) (PREPRODYNORPHIN) - BOS TAURUS (BOVINE). 
NDDB_CAVPO BETA-NEOENDORPHIN-DYNORPHIN PRECURSOR (PROENKEPHALIN B) (PREPRODYNORPHIN) - CAVIA PORCELLUS (GUINEA PIG).
NDDB_HUMAN BETA-NEOENDORPHIN-DYNORPHIN PRECURSOR (PROENKEPHALIN B) (PREPRODYNORPHIN) - HOMO SAPIENS (HUMAN).
NDDB_PIG BETA-NEOENDORPHIN-DYNORPHIN PRECURSOR (PROENKEPHALIN B) (PREPRODYNORPHIN) - SUS SCROFA (PIG).
NDDB_RAT BETA-NEOENDORPHIN-DYNORPHIN PRECURSOR (PROENKEPHALIN B) (PREPRODYNORPHIN) - RATTUS NORVEGICUS (RAT).
O35417 PRODYNORPHIN - MUS MUSCULUS (MOUSE).
Scan History
OWL30_2    2  100  NSINGLE    
SPTR37_9f 2 7 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
AWQGLLLAACLLVLPSTMA NDDB_PIG 2 2 -
AWQGLVLAACLLMFPSTTA NDDB_HUMAN 2 2 -
VWQGLVLAACLLALPSVTA NDDB_BOVIN 2 2 -
AWSRLMLAACLLVIPSEVA NDDB_RAT 2 2 -
VWPRLVLAACLLAMPPAAA NDDB_CAVPO 2 2 -

Motif 2 width=15
Element Seqn Id St Int Rpt
AVKTQDGPKPINPLI NDDB_PIG 30 9 -
AVKTQDGPKPINPLI NDDB_HUMAN 30 9 -
AVKTQDGPQPINPLV NDDB_BOVIN 30 9 -
AVRTQDGPHPINPLI NDDB_RAT 31 10 -
AVRTQDGPKPINPLI NDDB_CAVPO 30 9 -

Motif 3 width=11
Element Seqn Id St Int Rpt
QAALQPAEEWE NDDB_PIG 50 5 -
QAALLPSEEWE NDDB_HUMAN 50 5 -
QAALQPAQEWE NDDB_BOVIN 50 5 -
QDLVPPSEEWE NDDB_RAT 51 5 -
QETLLPSEEWE NDDB_CAVPO 50 5 -

Motif 4 width=18
Element Seqn Id St Int Rpt
KYMGPFLKELEKNRFLLS NDDB_PIG 97 36 -
KLSGSFLKELEKSKFLPS NDDB_HUMAN 97 36 -
KHIRPYLKELEKNRFLLS NDDB_BOVIN 97 36 -
KLLEPLLKELEKGQLLTS NDDB_RAT 98 36 -
KRARPFLQELEKSRFLPS NDDB_CAVPO 96 35 -

Motif 5 width=14
Element Seqn Id St Int Rpt
ESELMGDAQQNDGA NDDB_PIG 142 27 -
ESELMRDAQLNDGA NDDB_HUMAN 142 27 -
ESELMGDAQLNDGA NDDB_BOVIN 142 27 -
ESELLGTDLMNDEA NDDB_RAT 133 17 -
GSEAMGAPQLNSGA NDDB_CAVPO 132 18 -

Motif 6 width=14
Element Seqn Id St Int Rpt
LRRIRPKLKWDNQK NDDB_PIG 213 57 -
LRRIRPKLKWDNQK NDDB_HUMAN 211 55 -
LRRIRPKLKWDNQK NDDB_BOVIN 215 59 -
LRRIRPKLKWDNQK NDDB_RAT 206 59 -
LRRIRPKLKWDNQK NDDB_CAVPO 202 56 -

Motif 7 width=19
Element Seqn Id St Int Rpt
QFKVVTRSQEDPNAYYEEL NDDB_PIG 235 8 -
QFKVVTRSQEDPNAYSGEL NDDB_HUMAN 233 8 -
QFKVVTRSQEDPSAYYEEL NDDB_BOVIN 237 8 -
QFKVVTRSQENPNTYSEDL NDDB_RAT 228 8 -
QFKVVTRSQEDPNAYSEEF NDDB_CAVPO 224 8 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
AWQGLLLAACLLVLPSTMA NDDB_PIG 2 2 -
AWQGLVLAACLLMFPSTTA NDDB_HUMAN 2 2 -
VWQGLVLAACLLALPSVTA NDDB_BOVIN 2 2 -
AWSRLMLAACLLVIPSEVA NDDB_RAT 2 2 -
AWSRLMLAACLLVMPSNVM O35417 2 2 -
VWPRLVLAACLLAMPPAAA NDDB_CAVPO 2 2 -

Motif 2 width=15
Element Seqn Id St Int Rpt
AVKTQDGPKPINPLI NDDB_PIG 30 9 -
AVKTQDGPKPINPLI NDDB_HUMAN 30 9 -
AVKTQDGPQPINPLV NDDB_BOVIN 30 9 -
AVRTQDGPHPINPLI NDDB_RAT 31 10 -
AVRIQDGPRPINPLI O35417 31 10 -
AVRTQDGPKPINPLI NDDB_CAVPO 30 9 -

Motif 3 width=11
Element Seqn Id St Int Rpt
QAALQPAEEWE NDDB_PIG 50 5 -
QAALLPSEEWE NDDB_HUMAN 50 5 -
QAALQPAQEWE NDDB_BOVIN 50 5 -
QDLVPPSEEWE NDDB_RAT 51 5 -
QDLVPPSEEWE O35417 51 5 -
QETLLPSEEWE NDDB_CAVPO 50 5 -

Motif 4 width=18
Element Seqn Id St Int Rpt
KYMGPFLKELEKNRFLLS NDDB_PIG 97 36 -
KLSGSFLKELEKSKFLPS NDDB_HUMAN 97 36 -
KHIRPYLKELEKNRFLLS NDDB_BOVIN 97 36 -
KLLEPLLKELEKGQLLTS NDDB_RAT 98 36 -
KLLEPVLKELEKSRLLTS O35417 98 36 -
KRARPFLQELEKSRFLPS NDDB_CAVPO 96 35 -

Motif 5 width=14
Element Seqn Id St Int Rpt
ESELMGDAQQNDGA NDDB_PIG 142 27 -
ESELMRDAQLNDGA NDDB_HUMAN 142 27 -
ESELMGDAQLNDGA NDDB_BOVIN 142 27 -
ESELLGTDLMNDEA NDDB_RAT 133 17 -
ESELAGADRMNDEA O35417 133 17 -
GSEAMGAPQLNSGA NDDB_CAVPO 132 18 -

Motif 6 width=14
Element Seqn Id St Int Rpt
LRRIRPKLKWDNQK NDDB_PIG 213 57 -
LRRIRPKLKWDNQK NDDB_HUMAN 211 55 -
LRRIRPKLKWDNQK NDDB_BOVIN 215 59 -
LRRIRPKLKWDNQK NDDB_RAT 206 59 -
LRRIRPKLKWDNQK O35417 206 59 -
LRRIRPKLKWDNQK NDDB_CAVPO 202 56 -

Motif 7 width=19
Element Seqn Id St Int Rpt
QFKVVTRSQEDPNAYYEEL NDDB_PIG 235 8 -
QFKVVTRSQEDPNAYSGEL NDDB_HUMAN 233 8 -
QFKVVTRSQEDPSAYYEEL NDDB_BOVIN 237 8 -
QFKVVTRSQENPNTYSEDL NDDB_RAT 228 8 -
QFKVVTRSQENPNTYSEDL O35417 228 8 -
QFKVVTRSQEDPNAYSEEF NDDB_CAVPO 224 8 -