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PR01025

Identifier
NEUROKININ2R  [View Relations]  [View Alignment]  
Accession
PR01025
No. of Motifs
9
Creation Date
29-NOV-1998  (UPDATE 07-JUN-1999)
Title
Neurokinin NK2 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00244 NEUROKININR
INTERPRO; IPR000913
GCRDB; GCR_0081; GCR_0084; GCR_0156; GCR_0008; GCR_0243
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. YOKOTA, Y., SASAI, Y., TANAKA, K., FUJIWARA, T, TSUCHIDA, K., 
SHIGEMOTO, R., KAKIZUKA, A., OHKUBO, H. AND NAKANISHI, S.
Molecular characterisation of a functional cDNA for rat substance P
receptor.
J.BIOL.CHEM. 264(30) 17649-17652 (1989).
 
7. WATSON, S. AND ARKINSTALL, S.
Tachykinins.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.261-70.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Neuropeptide receptors are present in very small quantities in the cell
and are embedded tightly in the plasma membrane. The neuropeptides exhibit
a high degree of functional diversity through both regulation of peptide
production and through peptide-receptor interaction [6]. The mammalian
tachykinin system consists of 3 distinct peptides: substance P, substance
K and neuromedin K. All possess a common spectrum of biological activities,
including sensory transmission in the nervous system and contraction/
relaxation of peripheral smooth muscles, and each interacts with a 
specific receptor type.
 
In the periphery, NK2 receptors are found in smooth muscle of the
respiratory, gastrointestinal and urogenital systems [7]. NK2 receptors
activate the phosphoinositide pathway through a pertussis-toxin-insensitive 
G protein, probably of the Gq/G11 class [7].
 
NEUROKININ2R is a 9-element fingerprint that provides a signature for the
neurokinin NK2 receptors. The fingerprint was derived from an initial 
alignment of 5 sequences: the motifs were drawn from conserved sections 
within either loop or TM regions, focusing on those areas of the alignment
that characterise the NK2 receptors but distinguish them from the rest of
the neurokinin family - motifs 1 and 2 reside at the N-terminus; motifs 3
and 4 span the second external loop, leading into TM domain 5; motif 5 
resides in the third cytoplasmic loop; motif 6 lies in the third external
loop; and motifs 7-9 reside at the C-terminus. Two iterations on OWL30.2 
were required to reach convergence, at which point at true set comprising 
8 sequences was identified.
 
An update on SPTR37_9f identified a true set of 7 sequences.
Summary Information
7 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9777777777
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
NK2R_BOVIN    NK2R_CAVPO    NK2R_HUMAN    NK2R_MESAU    
NK2R_MOUSE NK2R_RABIT NK2R_RAT
Sequence Titles
NK2R_BOVIN  Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Bos taurus (Bovine). 
NK2R_CAVPO Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Cavia porcellus (Guinea pig).
NK2R_HUMAN Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Homo sapiens (Human).
NK2R_MESAU Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Mesocricetus auratus (Golden hamster).
NK2R_MOUSE Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Mus musculus (Mouse).
NK2R_RABIT Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Oryctolagus cuniculus (Rabbit).
NK2R_RAT Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Rattus norvegicus (Rat).
Scan History
OWL30_2    2  100  NSINGLE    
SPTR37_9f 2 8 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
GTRAIVSDANILSG NK2R_RAT 2 2 -
GAHASVTDTNILSG NK2R_MOUSE 2 2 -
GGRAIVTDTNIFSG NK2R_MESAU 2 2 -
GTCDIVTEANISSG NK2R_HUMAN 2 2 -
GACVVMTDINISSG NK2R_BOVIN 2 2 -

Motif 2 width=14
Element Seqn Id St Int Rpt
GLESNATGVTAFSM NK2R_RAT 15 -1 -
GLESNATGVTAFSM NK2R_MOUSE 15 -1 -
GLESNTTGVTAFSM NK2R_MESAU 15 -1 -
GPESNTTGITAFSM NK2R_HUMAN 15 -1 -
GLDSNATGITAFSM NK2R_BOVIN 15 -1 -

Motif 3 width=13
Element Seqn Id St Int Rpt
FYSTITVDEGATK NK2R_RAT 168 139 -
FYSTITVDQGATK NK2R_MOUSE 168 139 -
FYSTITVDQGATK NK2R_MESAU 168 139 -
FYSTVTMDQGATK NK2R_HUMAN 168 139 -
FYSTITTDEGATK NK2R_BOVIN 168 139 -

Motif 4 width=15
Element Seqn Id St Int Rpt
VVAWPNDNGGKMLLL NK2R_RAT 182 1 -
VVAWPNDNGGKMLLL NK2R_MOUSE 182 1 -
VVAWPNDNGGKMLLL NK2R_MESAU 182 1 -
VVAWPEDSGGKTLLL NK2R_HUMAN 182 1 -
VVAWPEDSGGKMLLL NK2R_BOVIN 182 1 -

Motif 5 width=19
Element Seqn Id St Int Rpt
KRAVPRHQAHGANLRHLQA NK2R_RAT 226 29 -
KRAVPRHQAHGANLRHLQA NK2R_MOUSE 226 29 -
KRAVPRHQAHGANLRHLHA NK2R_MESAU 226 29 -
RRAVPGHQAHGANLRHLQA NK2R_HUMAN 226 29 -
RRSVPGHQAHGANLRHLQA NK2R_BOVIN 226 29 -

Motif 6 width=13
Element Seqn Id St Int Rpt
ILGTFQEDIYYHK NK2R_RAT 271 26 -
ILGTFQEDIYYRK NK2R_MOUSE 271 26 -
ILGSFQKDIYYRK NK2R_MESAU 271 26 -
ILGSFQEDIYCHK NK2R_HUMAN 271 26 -
ILGTFQEDIYCHK NK2R_BOVIN 271 26 -

Motif 7 width=20
Element Seqn Id St Int Rpt
PWVTPTEEDRLELTHTPSLS NK2R_RAT 326 42 -
PWGTPTEEDRLELTHTPSIS NK2R_MOUSE 326 42 -
PWVTPTEEDRLELTRTPSLS NK2R_MESAU 326 42 -
PWVTPTKEDKLELTPTTSLS NK2R_HUMAN 326 42 -
PWVTPTEEDKMELTYTPSLS NK2R_BOVIN 326 42 -

Motif 8 width=15
Element Seqn Id St Int Rpt
SRRVNRCHTKETLFM NK2R_RAT 345 -1 -
SRRVNRCHTKETLFM NK2R_MOUSE 345 -1 -
SRRVNRCHTKETLFM NK2R_MESAU 345 -1 -
STRVNRCHTKETLFM NK2R_HUMAN 345 -1 -
STRVNRCHTKEIFFM NK2R_BOVIN 345 -1 -

Motif 9 width=19
Element Seqn Id St Int Rpt
MTGDMTHSEATNGQVGSPQ NK2R_RAT 359 -1 -
MTGDMTHSEATNGQVGGPQ NK2R_MOUSE 359 -1 -
MTADMTHSEATNGQVGSPQ NK2R_MESAU 359 -1 -
MAGDTAPSEATSGEAGRPQ NK2R_HUMAN 359 -1 -
MSGDVAPSEAVNGQAESPQ NK2R_BOVIN 359 -1 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
GTRAIVSDANILSG NK2R_RAT 2 2 -
GAHASVTDTNILSG NK2R_MOUSE 2 2 -
GGRAIVTDTNIFSG NK2R_MESAU 2 2 -
GACVIVTNTNISSG NK2R_CAVPO 2 2 -
GTCDIVTEANISSG NK2R_HUMAN 2 2 -
GACVVMTDINISSG NK2R_BOVIN 2 2 -
GACDIVTEANISSD NK2R_RABIT 2 2 -

Motif 2 width=14
Element Seqn Id St Int Rpt
GLESNATGVTAFSM NK2R_RAT 15 -1 -
GLESNATGVTAFSM NK2R_MOUSE 15 -1 -
GLESNTTGVTAFSM NK2R_MESAU 15 -1 -
GLESNTTGITAFSM NK2R_CAVPO 15 -1 -
GPESNTTGITAFSM NK2R_HUMAN 15 -1 -
GLDSNATGITAFSM NK2R_BOVIN 15 -1 -
DIDSNATGVTAFSM NK2R_RABIT 15 -1 -

Motif 3 width=13
Element Seqn Id St Int Rpt
FYSTITVDEGATK NK2R_RAT 168 139 -
FYSTITVDQGATK NK2R_MOUSE 168 139 -
FYSTITVDQGATK NK2R_MESAU 168 139 -
FYSTITEDEGATK NK2R_CAVPO 168 139 -
FYSTVTMDQGATK NK2R_HUMAN 168 139 -
FYSTITTDEGATK NK2R_BOVIN 168 139 -
FYSTITMDQGATK NK2R_RABIT 168 139 -

Motif 4 width=15
Element Seqn Id St Int Rpt
VVAWPNDNGGKMLLL NK2R_RAT 182 1 -
VVAWPNDNGGKMLLL NK2R_MOUSE 182 1 -
VVAWPNDNGGKMLLL NK2R_MESAU 182 1 -
VVAWPEDSRDKSLLL NK2R_CAVPO 182 1 -
VVAWPEDSGGKTLLL NK2R_HUMAN 182 1 -
VVAWPEDSGGKMLLL NK2R_BOVIN 182 1 -
VVAWPEDSGGKMLLL NK2R_RABIT 182 1 -

Motif 5 width=19
Element Seqn Id St Int Rpt
KRAVPRHQAHGANLRHLQA NK2R_RAT 226 29 -
KRAVPRHQAHGANLRHLQA NK2R_MOUSE 226 29 -
KRAVPRHQAHGANLRHLHA NK2R_MESAU 226 29 -
RRAVPRHQAHGANLRHLQA NK2R_CAVPO 226 29 -
RRAVPGHQAHGANLRHLQA NK2R_HUMAN 226 29 -
RRSVPGHQAHGANLRHLQA NK2R_BOVIN 226 29 -
RRTVPGHQTHGANLRHLRA NK2R_RABIT 226 29 -

Motif 6 width=13
Element Seqn Id St Int Rpt
ILGTFQEDIYYHK NK2R_RAT 271 26 -
ILGTFQEDIYYRK NK2R_MOUSE 271 26 -
ILGSFQKDIYYRK NK2R_MESAU 271 26 -
ILGSFQEDIYCHK NK2R_CAVPO 271 26 -
ILGSFQEDIYCHK NK2R_HUMAN 271 26 -
ILGTFQEDIYCHK NK2R_BOVIN 271 26 -
LLGHFQDDIYCRK NK2R_RABIT 271 26 -

Motif 7 width=20
Element Seqn Id St Int Rpt
PWVTPTEEDRLELTHTPSLS NK2R_RAT 326 42 -
PWGTPTEEDRLELTHTPSIS NK2R_MOUSE 326 42 -
PWVTPTEEDRLELTRTPSLS NK2R_MESAU 326 42 -
PWVTPTEEDKLELTHTPSFS NK2R_CAVPO 326 42 -
PWVTPTKEDKLELTPTTSLS NK2R_HUMAN 326 42 -
PWVTPTEEDKMELTYTPSLS NK2R_BOVIN 326 42 -
PWVTPTEEDKLELTHTPSLS NK2R_RABIT 326 42 -

Motif 8 width=15
Element Seqn Id St Int Rpt
SRRVNRCHTKETLFM NK2R_RAT 345 -1 -
SRRVNRCHTKETLFM NK2R_MOUSE 345 -1 -
SRRVNRCHTKETLFM NK2R_MESAU 345 -1 -
SLRVNRCHTKEILFM NK2R_CAVPO 345 -1 -
STRVNRCHTKETLFM NK2R_HUMAN 345 -1 -
STRVNRCHTKEIFFM NK2R_BOVIN 345 -1 -
SVRVNRCHTKETLFL NK2R_RABIT 345 -1 -

Motif 9 width=19
Element Seqn Id St Int Rpt
MTGDMTHSEATNGQVGSPQ NK2R_RAT 359 -1 -
MTGDMTHSEATNGQVGGPQ NK2R_MOUSE 359 -1 -
MTADMTHSEATNGQVGSPQ NK2R_MESAU 359 -1 -
MAGDTVPSEATNGQAGGPQ NK2R_CAVPO 359 -1 -
MAGDTAPSEATSGEAGRPQ NK2R_HUMAN 359 -1 -
MSGDVAPSEAVNGQAESPQ NK2R_BOVIN 359 -1 -
LVGDVAPSEAANGQAGGPQ NK2R_RABIT 359 -1 -