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PR00921

Identifier
IGASERPTASE  [View Relations]  [View Alignment]  
Accession
PR00921
No. of Motifs
9
Creation Date
18-JUN-1998  (UPDATE 10-JUN-1999)
Title
IgA-specific serine endopeptidase (S6) signature
Database References

INTERPRO; IPR000710
Literature References
1. RAWLINGS, N.D. AND BARRETT, A.J.
Families of serine peptidases.
METHODS ENZYMOL. 244 19-61 (1994).
 
2. RAWLINGS, N.D. AND BARRETT, A.J.
Evolutionary families of peptidases.
BIOCHEM.J. 290 205-218 (1993).
 
3. BAIROCH, A. AND RAWLINGS, N.
Classification of peptidase families and index of peptidase entries in
SWISS-PROT.
http://expasy.hcuge.ch/cgi-bin/lists?peptidas.txt

Documentation
Proteolytic enzymes that use serine in their catalytic machinery are 
widespread and numerous, being found in viruses, bacteria and eukaryotes
[1]. They encompass a range of peptidase activity, including exopeptidase,
endopeptidase, oligopeptidase and omega-peptidase. More than 20 serine
protease families (denoted S1 - S27) have been identified, which have been
grouped into 6 clans (SA, SB, SC, SE, SF and SG) on the basis of structural
and functional similarities [1]. Structures from four clans have been
examined (SA, SB, SC and SE): these appear to be unrelated, suggesting at 
least four evolutionary origins of serine peptidase, and possibly many more
[1]. Since that examination, structural representations from the other two 
clan members (SF, SG) have been determined [3].
 
Notwithstanding their different evolutionary origins, there are similarities
in the reaction mechanisms of several peptidases. Chymotrypsin, subtilisin
and carboxypeptidase C clans have a catalytic triad of serine, aspartate and
histidine in common: serine acts as a nucleophile, aspartate as an
electrophile, and histidine as a base [1]. The geometric orientations of
the catalytic residues are similar between families, despite different 
protein folds [1]. The linear arrangements of the catalytic residues
commonly reflect clan relationships. For example the catalytic triad in 
the chymotrypsin clan (SA) is ordered HDS, but is ordered DHS in the
subtilisin clan (SB) and SDH in the carboxypeptidase clan (SC) [1,2].
 
Proteins from the chymotrypsin clan (SA) have a conserved GXSG pattern at
the active serine site. The S6 family of the SA clan include IgA-specific
endopeptidases [1,3], which cleave prolyl bonds in the hinge regions of
immunoglobulin A heavy chains. Similar specificity is shown by the unrelated
family of M26 metalloendopeptidases. 
 
IGASERPTASE is a 9-element fingerprint that provides a signature for the 
IgA-specific endopeptidase (S6) family of serine peptidases. The fingerprint
was derived from an initial alignment of 8 sequences: the motifs were
drawn from conserved regions spanning virtually the full alignment length -
motif 2 includes the catalytic Asp; and motif 3 encodes the catalytically
active Ser. Two iterations on OWL30.1 were required to reach convergence,
at which point a true set comprising 18 sequences was identified. Numerous
partial matches were also found, all of which are fragments: B41500 matches
motifs 1-3; and 16 sequences match motifs 2 and 3 only.
 
An update on SPTR37_9f identified a true set of 9 sequences, and 7
partial matches.
Summary Information
   9 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
3 codes involving 4 elements
2 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
9999999999
8000000000
7000000000
6000000000
5000000000
4333003000
3222000000
2202000000
123456789
True Positives
HAP1_HAEIN    HAP_HAEIN     IGA0_HAEIN    IGA1_HAEIN    
IGA2_HAEIN IGA3_HAEIN IGA4_HAEIN IGA_NEIGO
Q51169
True Positive Partials
Codes involving 4 elements
O88093 Q47692 Q54151
Codes involving 3 elements
P77070 Q54165
Codes involving 2 elements
O32555 O32591
Sequence Titles
HAP1_HAEIN  ADHESION AND PENETRATION PROTEIN PRECURSOR (EC 3.4.21.-) - HAEMOPHILUS INFLUENZAE. 
HAP_HAEIN ADHESION AND PENETRATION PROTEIN PRECURSOR (EC 3.4.21.-) - HAEMOPHILUS INFLUENZAE.
IGA0_HAEIN IMMUNOGLOBULIN A1 PROTEASE PRECURSOR (EC 3.4.21.72) (IGA1 PROTEASE) - HAEMOPHILUS INFLUENZAE.
IGA1_HAEIN IMMUNOGLOBULIN A1 PROTEASE PRECURSOR (EC 3.4.21.72) (IGA1 PROTEASE) - HAEMOPHILUS INFLUENZAE.
IGA2_HAEIN IMMUNOGLOBULIN A1 PROTEASE PRECURSOR (EC 3.4.21.72) (IGA1 PROTEASE) - HAEMOPHILUS INFLUENZAE.
IGA3_HAEIN IMMUNOGLOBULIN A1 PROTEASE PRECURSOR (EC 3.4.21.72) (IGA1 PROTEASE) - HAEMOPHILUS INFLUENZAE.
IGA4_HAEIN IMMUNOGLOBULIN A1 PROTEASE PRECURSOR (EC 3.4.21.72) (IGA1 PROTEASE) - HAEMOPHILUS INFLUENZAE.
IGA_NEIGO IGA-SPECIFIC SERINE ENDOPEPTIDASE PRECURSOR (EC 3.4.21.72) (IGA PROTEASE) - NEISSERIA GONORRHOEAE.
Q51169 IGA1 PROTEASE - NEISSERIA MENINGITIDIS.

O88093 HAEMOGLOBIN PROTEASE PRECURSOR - ESCHERICHIA COLI.
Q47692 TSH - ESCHERICHIA COLI.
Q54151 HEMAGGLUTININ - SHIGELLA FLEXNERI.

P77070 ESPC - ESCHERICHIA COLI.
Q54165 SEPA GENE PRECURSOR - SHIGELLA FLEXNERI.

O32555 PUTATIVE EXOPROTEIN-PRECURSOR - ESCHERICHIA COLI.
O32591 PROTEASE - ESCHERICHIA COLI.
Scan History
OWL30_1    2  50   NSINGLE    
SPTR37_9f 2 35 NSINGLE
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
DYQIFRDFAENKGKFSVGATN IGA1_HAEIN 33 33 -
DYQIFRDFAENKGRFSVGATN IGA0_HAEIN 33 33 -
DYQIFRDFAENKGRFSVGATN IGA2_HAEIN 33 33 -
DYQIFRDFAENKGKFSVGATN IGA3_HAEIN 33 33 -
DYQIFRDFAENKGKFSVGATN IGA4_HAEIN 33 33 -
DYQIFRDFAENKGKFFVGATD IGA_NEIGO 35 35 -
DYQYYRDFAENKGKFTVGAQN HAP_HAEIN 33 33 -
DYQYYRDFAENKGKFTVGAKN HAP1_HAEIN 33 33 -

Motif 2 width=21
Element Seqn Id St Int Rpt
DYYMPRLDKFVTEVAPIEAST IGA1_HAEIN 164 110 -
DYYMPRLDKFVTEVAPIEAST IGA0_HAEIN 164 110 -
DYYMPRLDKFVTEVAPIEAST IGA2_HAEIN 164 110 -
DYYMPRLDKFVTEVAPIEAST IGA3_HAEIN 164 110 -
DYYMPRLDKFVTEVAPIEAST IGA4_HAEIN 165 111 -
DYNMARFNKFVTEVAPIAPTD IGA_NEIGO 151 95 -
DYHNPRLHKFVTEAAPIDMTS HAP_HAEIN 140 86 -
DYHMPRLHKFVTEAEPVGMTT HAP1_HAEIN 141 87 -

Motif 3 width=19
Element Seqn Id St Int Rpt
GDSGSPLFVYDREKGKWLF IGA1_HAEIN 286 101 -
GDSGSPLFVYDREKGKWLF IGA0_HAEIN 286 101 -
GDSGSPLFVYDREKGKWLF IGA2_HAEIN 286 101 -
GDSGSPLFVYDREKGKWLF IGA3_HAEIN 290 105 -
GDSGSPLFVYDREKGKWLF IGA4_HAEIN 297 111 -
GDSGSPLFAFDKQKNQWVF IGA_NEIGO 276 104 -
GDSGSPMFIYDAEKQKWLI HAP_HAEIN 241 80 -
GDSGSPMFIYDAKKKQWLI HAP1_HAEIN 248 86 -

Motif 4 width=18
Element Seqn Id St Int Rpt
NHINNERMNGFNGYFGEE IGA1_HAEIN 642 337 -
NHINNERMNGFNGYFGEE IGA0_HAEIN 648 343 -
NHINNERMNGFNGYFGEE IGA2_HAEIN 648 343 -
NHINNERMNGFNGYFGEE IGA3_HAEIN 646 337 -
NHINNERMNGFNGYFGEE IGA4_HAEIN 653 337 -
NHKNNRRIGDFGGFFDEE IGA_NEIGO 631 336 -
NKLDYRKEIAYNGWFGET HAP_HAEIN 554 294 -
NKLDYSKEIAYNGWFGET HAP1_HAEIN 571 304 -

Motif 5 width=23
Element Seqn Id St Int Rpt
NGNLNVTFKGKSEQNRFLLTGGT IGA1_HAEIN 664 4 -
NGNLNVTFKGKSEQNRFLLTGGT IGA0_HAEIN 670 4 -
NGNLNVTFKGKSEQNRFLLTGGT IGA2_HAEIN 670 4 -
NGNLNVTFKGKSEQNRFLLTGGT IGA3_HAEIN 668 4 -
NGKLNVTFNGKSDQNRFLLTGGT IGA4_HAEIN 677 6 -
NGALNLNFNGKSAQKRFLLTGGA IGA_NEIGO 654 5 -
NGRLNLIYKPTTEDRTLLLSGGT HAP_HAEIN 577 5 -
NGRLNLIYKPTTEDRTLLLSGGT HAP1_HAEIN 594 5 -

Motif 6 width=23
Element Seqn Id St Int Rpt
NLNGDLTVEKGTLFLSGRPTPHA IGA1_HAEIN 687 0 -
NLNGDLKVEKGTLFLSGRPTPHA IGA0_HAEIN 693 0 -
NLNGDLKVEKGTLFLSGRPTPHA IGA2_HAEIN 693 0 -
NLNGDLNVQQGTLFLSGRPTPHA IGA3_HAEIN 691 0 -
NLNGDLNVEKGTLFLSGRPTPHA IGA4_HAEIN 700 0 -
NLNGKISVTQGNVLLSGRPTPHA IGA_NEIGO 677 0 -
NLKGDITQTKGKLFFSGRPTPHA HAP_HAEIN 600 0 -
NLKGDITQTKGKLFFSGRPTPHA HAP1_HAEIN 617 0 -

Motif 7 width=15
Element Seqn Id St Int Rpt
EVVVEDDWINRNFKA IGA1_HAEIN 729 19 -
EVVVEDDWINRNFKA IGA0_HAEIN 735 19 -
EVVVEDDWINRNFKA IGA2_HAEIN 735 19 -
EVVVEDDWINRNFKA IGA3_HAEIN 733 19 -
EVVVEDDWINRNFKA IGA4_HAEIN 742 19 -
EVVFEDDWINRTFKA IGA_NEIGO 719 19 -
EIVWDHDWINRTFKA HAP_HAEIN 640 17 -
EIVWDYDWINRTFKA HAP1_HAEIN 657 17 -

Motif 8 width=20
Element Seqn Id St Int Rpt
VCVRSDYTGYVTCTTDKLSD IGA1_HAEIN 785 41 -
VCVRSDYTGYVTCTTDKLSD IGA0_HAEIN 791 41 -
VCVRSDYTGYVTCTTDKLSD IGA2_HAEIN 791 41 -
VCVRSDYTGYVTCTTDKLSD IGA3_HAEIN 789 41 -
VCVRSDYTGYVTCHNSNLSE IGA4_HAEIN 798 41 -
VCVRSDYTGYVTCNTGNLSD IGA_NEIGO 775 41 -
ICTRSDWTGLTTCQKVDLTD HAP_HAEIN 697 42 -
ICTRSDWTGLTTCKTVNLTD HAP1_HAEIN 714 42 -

Motif 9 width=15
Element Seqn Id St Int Rpt
VDLGAWKYKLRNVNG IGA1_HAEIN 962 157 -
VDLGAWKYKLRNVNG IGA0_HAEIN 968 157 -
VDLGAWKYKLRNVNG IGA2_HAEIN 968 157 -
VDLGAWKYKLRNVNG IGA3_HAEIN 966 157 -
VDRGAWKYKLRNVNG IGA4_HAEIN 975 157 -
VDLGALRYTIKTENG IGA_NEIGO 953 158 -
VDAGALRYKLVKNDG HAP_HAEIN 955 238 -
VDAGALRYKLVKNKG HAP1_HAEIN 973 239 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
DYQIFRDFAENKGKFSVGATN IGA1_HAEIN 33 33 -
DYQIFRDFAENKGRFSVGATN IGA0_HAEIN 33 33 -
DYQIFRDFAENKGRFSVGATN IGA2_HAEIN 33 33 -
DYQIFRDFAENKGKFSVGATN IGA3_HAEIN 33 33 -
DYQIFRDFAENKGKFSVGATN IGA4_HAEIN 33 33 -
DYQIFRDFAENKGKFFVGATD Q51169 35 35 -
DYQIFRDFAENKGKFFVGATD IGA_NEIGO 35 35 -
DYQYYRDFAENKGKFTVGAQN HAP_HAEIN 33 33 -
DYQYYRDFAENKGKFTVGAKN HAP1_HAEIN 33 33 -

Motif 2 width=21
Element Seqn Id St Int Rpt
DYYMPRLDKFVTEVAPIEAST IGA1_HAEIN 164 110 -
DYYMPRLDKFVTEVAPIEAST IGA0_HAEIN 164 110 -
DYYMPRLDKFVTEVAPIEAST IGA2_HAEIN 164 110 -
DYYMPRLDKFVTEVAPIEAST IGA3_HAEIN 164 110 -
DYYMPRLDKFVTEVAPIEAST IGA4_HAEIN 165 111 -
DYNMARFNKFVTEVAPIAPTD Q51169 151 95 -
DYNMARFNKFVTEVAPIAPTD IGA_NEIGO 151 95 -
DYHNPRLHKFVTEAAPIDMTS HAP_HAEIN 140 86 -
DYHMPRLHKFVTEAEPVGMTT HAP1_HAEIN 141 87 -

Motif 3 width=19
Element Seqn Id St Int Rpt
GDSGSPLFVYDREKGKWLF IGA1_HAEIN 286 101 -
GDSGSPLFVYDREKGKWLF IGA0_HAEIN 286 101 -
GDSGSPLFVYDREKGKWLF IGA2_HAEIN 286 101 -
GDSGSPLFVYDREKGKWLF IGA3_HAEIN 290 105 -
GDSGSPLFVYDREKGKWLF IGA4_HAEIN 297 111 -
GDSGSPLFAFDKQKNQWVF Q51169 276 104 -
GDSGSPLFAFDKQKNQWVF IGA_NEIGO 276 104 -
GDSGSPMFIYDAEKQKWLI HAP_HAEIN 241 80 -
GDSGSPMFIYDAKKKQWLI HAP1_HAEIN 248 86 -

Motif 4 width=18
Element Seqn Id St Int Rpt
NHINNERMNGFNGYFGEE IGA1_HAEIN 642 337 -
NHINNERMNGFNGYFGEE IGA0_HAEIN 648 343 -
NHINNERMNGFNGYFGEE IGA2_HAEIN 648 343 -
NHINNERMNGFNGYFGEE IGA3_HAEIN 646 337 -
NHINNERMNGFNGYFGEE IGA4_HAEIN 653 337 -
NHKNNQRISGFSGFFGEE Q51169 631 336 -
NHKNNRRIGDFGGFFDEE IGA_NEIGO 631 336 -
NKLDYRKEIAYNGWFGET HAP_HAEIN 554 294 -
NKLDYSKEIAYNGWFGET HAP1_HAEIN 571 304 -

Motif 5 width=23
Element Seqn Id St Int Rpt
NGNLNVTFKGKSEQNRFLLTGGT IGA1_HAEIN 664 4 -
NGNLNVTFKGKSEQNRFLLTGGT IGA0_HAEIN 670 4 -
NGNLNVTFKGKSEQNRFLLTGGT IGA2_HAEIN 670 4 -
NGNLNVTFKGKSEQNRFLLTGGT IGA3_HAEIN 668 4 -
NGKLNVTFNGKSDQNRFLLTGGT IGA4_HAEIN 677 6 -
NGALNLNFNGKSAQNRFLLTGGT Q51169 654 5 -
NGALNLNFNGKSAQKRFLLTGGA IGA_NEIGO 654 5 -
NGRLNLIYKPTTEDRTLLLSGGT HAP_HAEIN 577 5 -
NGRLNLIYKPTTEDRTLLLSGGT HAP1_HAEIN 594 5 -

Motif 6 width=23
Element Seqn Id St Int Rpt
NLNGDLTVEKGTLFLSGRPTPHA IGA1_HAEIN 687 0 -
NLNGDLKVEKGTLFLSGRPTPHA IGA0_HAEIN 693 0 -
NLNGDLKVEKGTLFLSGRPTPHA IGA2_HAEIN 693 0 -
NLNGDLNVQQGTLFLSGRPTPHA IGA3_HAEIN 691 0 -
NLNGDLNVEKGTLFLSGRPTPHA IGA4_HAEIN 700 0 -
NLNGKISVTQGNVLLSGRPTPHA Q51169 677 0 -
NLNGKISVTQGNVLLSGRPTPHA IGA_NEIGO 677 0 -
NLKGDITQTKGKLFFSGRPTPHA HAP_HAEIN 600 0 -
NLKGDITQTKGKLFFSGRPTPHA HAP1_HAEIN 617 0 -

Motif 7 width=15
Element Seqn Id St Int Rpt
EVVVEDDWINRNFKA IGA1_HAEIN 729 19 -
EVVVEDDWINRNFKA IGA0_HAEIN 735 19 -
EVVVEDDWINRNFKA IGA2_HAEIN 735 19 -
EVVVEDDWINRNFKA IGA3_HAEIN 733 19 -
EVVVEDDWINRNFKA IGA4_HAEIN 742 19 -
EVVFEDDWINRTFKA Q51169 719 19 -
EVVFEDDWINRTFKA IGA_NEIGO 719 19 -
EIVWDHDWINRTFKA HAP_HAEIN 640 17 -
EIVWDYDWINRTFKA HAP1_HAEIN 657 17 -

Motif 8 width=20
Element Seqn Id St Int Rpt
VCVRSDYTGYVTCTTDKLSD IGA1_HAEIN 785 41 -
VCVRSDYTGYVTCTTDKLSD IGA0_HAEIN 791 41 -
VCVRSDYTGYVTCTTDKLSD IGA2_HAEIN 791 41 -
VCVRSDYTGYVTCTTDKLSD IGA3_HAEIN 789 41 -
VCVRSDYTGYVTCHNSNLSE IGA4_HAEIN 798 41 -
VCVRSDYTGYVTCNTDNLSD Q51169 775 41 -
VCVRSDYTGYVTCNTGNLSD IGA_NEIGO 775 41 -
ICTRSDWTGLTTCQKVDLTD HAP_HAEIN 697 42 -
ICTRSDWTGLTTCKTVNLTD HAP1_HAEIN 714 42 -

Motif 9 width=15
Element Seqn Id St Int Rpt
VDLGAWKYKLRNVNG IGA1_HAEIN 962 157 -
VDLGAWKYKLRNVNG IGA0_HAEIN 968 157 -
VDLGAWKYKLRNVNG IGA2_HAEIN 968 157 -
VDLGAWKYKLRNVNG IGA3_HAEIN 966 157 -
VDRGAWKYKLRNVNG IGA4_HAEIN 975 157 -
VDLGALRYTIKTENG Q51169 953 158 -
VDLGALRYTIKTENG IGA_NEIGO 953 158 -
VDAGALRYKLVKNDG HAP_HAEIN 955 238 -
VDAGALRYKLVKNKG HAP1_HAEIN 973 239 -