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PR00897

Identifier
VASOPRSNV1BR  [View Relations]  [View Alignment]  
Accession
PR00897
No. of Motifs
9
Creation Date
15-APR-1998  (UPDATE 07-JUN-1999)
Title
Vasopressin VIB receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00896 VASOPRESSINR
INTERPRO; IPR000628
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Vasopressin and oxytocin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.284-291.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Vasopressin and oxytocin are members of the neurohypophyseal hormone family
found in all mammalian species [6]. They are present in high levels in the
posterior pituitary. Vasopressin has an essential role in the control of 
the water content of the body, acting in the kidney to increase water and
sodium absorption [6]. In higher concentrations, vasopressin stimulates
contraction of vascular smooth muscle, stimulates glycogen breakdown in the
liver, induces platelet activation, and evokes release of corticotrophin 
from the anterior pituitary [6]. Vasopressin and its analogues are used
clinically to treat diabetes insipidus [6]. 
 
In the periphery, the V1A receptor is found in high levels in vascular
smooth muscle, myometrium and the bladder where it mediates contraction [6].
V1B receptors can be distinguished from V1A receptors by the low affinity of
certain antagonists at the former. The receptors stimulate phosphoinositide
metabolism and are found in the anterior pituitary [6].
 
VASOPRSNV1BR is a 9-element fingerprint that provides a signature for
vasopressin V1B receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from short conserved
sections spanning the full alignment length, focusing on those regions
that characterise the vasopressin V1B receptors but distinguish them from
the rest of the vasopressin family - motif 1 lies at the N-terminus; motif 2
lies in the second cytoplasmic loop; motif 3 lies in the second external 
loop; motifs 4 and 5 span the third cytoplasmic loop; motif 6 lies in the
third external loop; and motifs 7-9 reside in the C-terminal domain. A 
single iteration on OWL30.1 was required to reach convergence, no further
sequences being identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 2 sequences.
Summary Information
2 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9222222222
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
V1BR_HUMAN    V1BR_RAT      
Sequence Titles
V1BR_HUMAN  Vasopressin V1b receptor (V1bR) (AVPR V1b) (Vasopressin V3 receptor) (AVPR V3) (Antidiuretic hormone receptor 1b) - Homo sapiens (Human). 
V1BR_RAT Vasopressin V1b receptor (V1bR) (AVPR V1b) (Vasopressin V3 receptor) (AVPR V3) (Antidiuretic hormone receptor 1b) - Rattus norvegicus (Rat).
Scan History
OWL30_1    1  50   NSINGLE    
SPTR37_9f 2 3 NSINGLE
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
PSWTATPSPGGTLPVPNATTPWL V1BR_RAT 5 5 -
PLWDANPTPRGTLSAPNATTPWL V1BR_HUMAN 5 5 -
PSWTATPSPGGTLPVPNATTPWL RNU27322 5 5 -

Motif 2 width=17
Element Seqn Id St Int Rpt
RSLRQPSQSTYPLIAAP RNU27322 141 113 -
RSLRQPSQSTYPLIAAP V1BR_RAT 141 113 -
RSLQQPGQSTYLLIAAP V1BR_HUMAN 141 113 -

Motif 3 width=13
Element Seqn Id St Int Rpt
SLREVIQGSGVLD V1BR_HUMAN 173 15 -
SLREVIQGSGVLD RNU27322 173 15 -
SLREVIQGSGVLD V1BR_RAT 173 15 -

Motif 4 width=20
Element Seqn Id St Int Rpt
TQAWRVGGGGWRTWDRPSPS V1BR_HUMAN 234 48 -
TQAGREERRGWRTWDKSSSS V1BR_RAT 234 48 -
TQAGREERRGWRTWDKSSSS RNU27322 234 48 -

Motif 5 width=16
Element Seqn Id St Int Rpt
AATRGLPSRVSSISTI V1BR_RAT 258 4 -
AATRGLPSRVSSISTI RNU27322 254 0 -
ATTRGLPSRVSSINTI V1BR_HUMAN 257 3 -

Motif 6 width=14
Element Seqn Id St Int Rpt
DENAPNEDSTNVAF V1BR_RAT 308 34 -
DENAPNEDSTNVAF RNU27322 304 34 -
DKNAPDEDSTNVAF V1BR_HUMAN 307 34 -

Motif 7 width=18
Element Seqn Id St Int Rpt
RQLSTSSLTSRRTTLLTH RNU27322 362 44 -
RRLSDGSLSSRHTTLLTR V1BR_HUMAN 365 44 -
RQLSTSSLTSRRTTLLTH V1BR_RAT 366 44 -

Motif 8 width=16
Element Seqn Id St Int Rpt
TLRLSLNLSLRAKPRP RNU27322 385 5 -
TLSLSLSLTLSGRPRP V1BR_HUMAN 388 5 -
TLRLSLNLSLRAKPRP V1BR_RAT 389 5 -

Motif 9 width=16
Element Seqn Id St Int Rpt
KDLEQVDGEATMETSI RNU27322 405 4 -
KDLEQVDGEATMETSI V1BR_RAT 409 4 -
RDLELADGEGTAETII V1BR_HUMAN 408 4 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
PLWDANPTPRGTLSAPNATTPWL V1BR_HUMAN 5 5 -
PSWTATPSPGGTLPVPNATTPWL V1BR_RAT 5 5 -

Motif 2 width=17
Element Seqn Id St Int Rpt
RSLQQPGQSTYLLIAAP V1BR_HUMAN 141 113 -
RSLRQPSQSTYPLIAAP V1BR_RAT 141 113 -

Motif 3 width=13
Element Seqn Id St Int Rpt
SLREVIQGSGVLD V1BR_HUMAN 173 15 -
SLREVIQGSGVLD V1BR_RAT 173 15 -

Motif 4 width=20
Element Seqn Id St Int Rpt
TQAWRVGGGGWRTWDRPSPS V1BR_HUMAN 234 48 -
TQAGREERRGWRTWDKSSSS V1BR_RAT 234 48 -

Motif 5 width=16
Element Seqn Id St Int Rpt
ATTRGLPSRVSSINTI V1BR_HUMAN 257 3 -
AATRGLPSRVSSISTI V1BR_RAT 258 4 -

Motif 6 width=14
Element Seqn Id St Int Rpt
DKNAPDEDSTNVAF V1BR_HUMAN 307 34 -
DENAPNEDSTNVAF V1BR_RAT 308 34 -

Motif 7 width=18
Element Seqn Id St Int Rpt
RRLSDGSLSSRHTTLLTR V1BR_HUMAN 365 44 -
RQLSTSSLTSRRTTLLTH V1BR_RAT 366 44 -

Motif 8 width=16
Element Seqn Id St Int Rpt
TLSLSLSLTLSGRPRP V1BR_HUMAN 388 5 -
TLRLSLNLSLRAKPRP V1BR_RAT 389 5 -

Motif 9 width=16
Element Seqn Id St Int Rpt
RDLELADGEGTAETII V1BR_HUMAN 408 4 -
KDLEQVDGEATMETSI V1BR_RAT 409 4 -