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PR00849

Identifier
GLHYDRLASE58  [View Relations]  [View Alignment]  
Accession
PR00849
No. of Motifs
8
Creation Date
05-MAR-1998  (UPDATE 21-JUN-1999)
Title
Glycosyl hydrolase family 58 signature
Database References

INTERPRO; IPR001724
Literature References
1. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
 
2. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
 
3. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
 
4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
Nucleotide sequences of the Arb genes, which control beta-glucosidase
utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
coli Bgl operon and evidence for a new beta-glycohydrolase family
including enzymes from eubacteria, archaebacteria and humans.
J.BACTERIOL. 174 765-777 (1992).
 
5. LONG, G.S., BRYANT, J.M., TAYLOR, P.W., LUZIO, J.P.
Complete nucleotide sequence of the gene encoding bacteriophage E 
endosialidase: implications for K1E endosialidase structure and function. 
BIOCHEM.J. 309 543-550 (1995).
 
6. PETTER, J.G. AND VIMR, E.R.
Complete nucleotide sequence of the bacteriophage K1F tail gene encoding 
endo-n-acylneuraminidase (endo-N) and comparison to an endo-N homolog in
bacteriophage PK1E. 
J.BACTERIOL. 175 4354-4363 (1993). 

Documentation
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 58 includes the endo-N-acetyl-neuraminidases (EC 
3.2.1.129).
 
Bacteriophage E specifically recognises and infects strains of E.coli
that display the alpha-2,8-linked polysialic acid K1 capsule [5,6]. 
Bacteriophage E endosialidase is thought to be responsible for initial
absorption of the phage to the host bacterium [5]. The native enzyme is
probably a trimer of identical 74kDa subunits. Within the K1E endosialidase
sequence, a central region of 500 amino acids shows 84% identity to K1F 
endosialidase [5]. Both enzymes contain two copies of a sialidase sequence
motif common to many bacterial and viral sialidases. These motifs flank
the region of greatest identity between the two endosialidases, suggesting
that this domain is involved in binding and hydrolysis of the polysialic
acid substrate [5].
 
GLHYDRLASE58 is an 8-element fingerprint that provides a signature for
family 58 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved regions
spanning the C-terminal half of the alignment. Two iterations on OWL30.0
were required to reach convergence, at which point a true set comprising 
3 sequences was identified.
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
833333333
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
ENAN_BPK1E    ENAN_BPK1F    O80102        
Sequence Titles
ENAN_BPK1E  ENDO-N-ACETYLNEURAMINIDASE (EC 3.2.1.129) (ENDO-N) (ENDOSIALIDASE) - BACTERIOPHAGE K1E. 
ENAN_BPK1F ENDO-N-ACETYLNEURAMINIDASE (EC 3.2.1.129) (ENDO-N) (ENDOSIALIDASE) (G102) - BACTERIOPHAGE K1F.
O80102 ENDO-N-ACETYLNEURAMINIDASE - BACTERIOPHAGE #D.
Scan History
OWL30_0    2  75   NSINGLE    
SPTR37_9f 2 20 NSINGLE
Initial Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
GELYKITDNPYYNAWPQDKAFVYEN ENAN_BPK1E 104 104 -
GELFKITDTPYYNAWPQDKAFVYEN ENAN_BPK1F 313 313 -

Motif 2 width=29
Element Seqn Id St Int Rpt
HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1E 169 40 -
HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1F 378 40 -

Motif 3 width=24
Element Seqn Id St Int Rpt
GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1E 288 90 -
GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1F 497 90 -

Motif 4 width=27
Element Seqn Id St Int Rpt
RRQIPSEYEPDAAEPCIKYYDGVLYLI ENAN_BPK1E 358 46 -
RRQIPSEYEPDASEPCIKYYDGVLYLI ENAN_BPK1F 567 46 -

Motif 5 width=27
Element Seqn Id St Int Rpt
ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1E 436 51 -
ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1F 645 51 -

Motif 6 width=25
Element Seqn Id St Int Rpt
VGVGSVVVKDSFIYYIFGGENHFNP ENAN_BPK1E 492 29 -
VGVGSVVVKDNYIYYMFGGEDHFNP ENAN_BPK1F 701 29 -

Motif 7 width=27
Element Seqn Id St Int Rpt
LKASTSANIRSEMQMEGEYGFIGKSVP ENAN_BPK1E 598 81 -
IRASTSSNIRSEVLMEGEYGFIGKSIP ENAN_BPK1F 802 76 -

Motif 8 width=28
Element Seqn Id St Int Rpt
GQRLIICGGEGTSSSSGAQITLHGSNSS ENAN_BPK1E 630 5 -
GQRIIFCGGEGTSSTTGAQITLYGANNT ENAN_BPK1F 834 5 -
Final Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
GELYKITDNPYYNAWPQDKAFVYEN ENAN_BPK1E 104 104 -
GELFKITDTPYYNAWPQDKAFVYEN ENAN_BPK1F 313 313 -
GSLTKVTDTPFYNAWTQDKTFVYDH O80102 235 235 -

Motif 2 width=29
Element Seqn Id St Int Rpt
HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1E 169 40 -
HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1F 378 40 -
SVSADRVNYHCMSMGTCGNRLYAVIETRY O80102 306 46 -

Motif 3 width=24
Element Seqn Id St Int Rpt
GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1E 288 90 -
GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1F 497 90 -
GVTWNFATRFWDSPWEITELPEVA O80102 425 90 -

Motif 4 width=27
Element Seqn Id St Int Rpt
RRQIPSEYEPDAAEPCIKYYDGVLYLI ENAN_BPK1E 358 46 -
RRQIPSEYEPDASEPCIKYYDGVLYLI ENAN_BPK1F 567 46 -
RRTVAQAYADNASEPCVKSYGGVLYLT O80102 504 55 -

Motif 5 width=27
Element Seqn Id St Int Rpt
ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1E 436 51 -
ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1F 645 51 -
ERAFGEWEGGAPDSRYEGSYPRTFMCK O80102 582 51 -

Motif 6 width=25
Element Seqn Id St Int Rpt
VGVGSVVVKDSFIYYIFGGENHFNP ENAN_BPK1E 492 29 -
VGVGSVVVKDNYIYYMFGGEDHFNP ENAN_BPK1F 701 29 -
VGVGSVCVKDGWLYYIFGGEDFFSP O80102 640 31 -

Motif 7 width=27
Element Seqn Id St Int Rpt
LKASTSANIRSEMQMEGEYGFIGKSVP ENAN_BPK1E 598 81 -
IRASTSSNIRSEVLMEGEYGFIGKSIP ENAN_BPK1F 802 76 -
LEYDGTQQSAVRARFDGDYGLISKTVP O80102 744 79 -

Motif 8 width=28
Element Seqn Id St Int Rpt
GQRLIICGGEGTSSSSGAQITLHGSNSS ENAN_BPK1E 630 5 -
GQRIIFCGGEGTSSTTGAQITLYGANNT ENAN_BPK1F 834 5 -
RQRLIVSGGDGASSSDGALLQLYGSNHA O80102 776 5 -