| Literature References | 1. MIZUKI, N. AND KASAHARA, M.
Mouse submandibular glands express an androgen-regulated transcript
encoding an acidic epididymal glycoprotein-like molecule.
MOL.CELL.ENDOCRINOL. 89 25-32 (1992).
2. KASAHARA, M., GUTKNECHT, J., BREW, K., SPURR, N. AND GOODFELLOW, P.N.
Cloning and mapping of a testis-specific gene with sequence similarity
to a sperm-coating glycoprotein gene.
GENOMICS 5 527-534 (1989).
3. LU, G., VILLALBA, M., COSCIA, M.R., HOFFMAN, D.R.and KING, T.P.
Sequence-analysis and antigenic cross-reactivity of a venom allergen,
antigen-5, from hornets, wasps, and yellow jackets.
J.IMMUNOL. 150 2823-2830 (1993).
4. DIXON, D.C., CUTT, J.R. AND KLESSIG, D.F.
Differential targeting of the tobacco pr-1 pathogenesis-related proteins
to the extracellular space and vacuoles of crystal dioblasts.
EMBO J. 10 1317-1324 (1991).
5. SCHUREN, F.H.J., ASGEIRSDOTTIR, S.A., KOTHE, E.M., SCHEER, J.M.J.
AND WESSELS, J.G.H.
The sc7/sc14 gene family of schizophyllum-commune codes for extracellular
proteins specifically expressed during fruit-body formation.
J.GEN.MICROBIOL. 139 2083-2090 (1993).
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| Documentation | A number of eukaryotic extracellular proteins have been shown to be
evolutionarily related. The family includes rodent sperm-coating glyco-
protein (or acidic epididymal glycoprotein), which is thought to be involved
in sperm maturation [1]; mammalian testis-specific protein (Tpx-1) [2];
mammalian glioma pathogenesis-related protein; lizard helothermine, a toxin
that blocks ryanodine receptors; venom allergen 5 from vespid wasps and
venom allergen 3 from fire ants, which are potent allergens that mediate
allergic reactions to stings from insects of the hymenoptera family [3];
plant pathogenesis proteins of the PR-1 family [4], which are synthesised
during pathogen infection or other stress-related responses; proteins Sc7
and Sc14 from the basidiomycete fungus Schizophyllum commune, which are
loosely associated with fruiting body hyphal walls [5]; ancylostoma secreted
protein from dog hookworm; and yeast hypothetical proteins YJL078c, YJL079c
and YKR013w. The precise functions of these proteins is still unclear.
V5TPXLIKE is a 4-element fingerprint that provides a signature for the
allergen V5/Tpx-1 family of extracellular proteins. The fingerprint was
derived from an initial alignment of 13 sequences: the motifs were drawn
from short conserved regions spanning the C-terminal half of the alignment -
motif 3 includes the region encoded by PROSITE pattern SCP_AG5_PR1_SC7_1
(PS01009); and motif 4 includes the region encoded by PROSITE pattern
SCP_AG5_PR1_SC7_2 (PS010010), which contains a Cys residue known to be
involved in disulphide bond formation in Ag5. Three iterations on OWL30.0
were required to reach convergence, at which point a true set comprising 86
sequences was identified. Several partial matches were also found, most of
which are uncharacterised sequences from the C.elegans genome.
An update on SPTR37_9f identified a true set of 95 sequences, and 21
partial matches.
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