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PR00659

Identifier
CHROMOGRANIN  [View Relations]  [View Alignment]  
Accession
PR00659
No. of Motifs
3
Creation Date
18-JAN-1997  (UPDATE 10-JUN-1999)
Title
Chromogranin signature
Database References

PROSITE; PS00422 GRANINS_1; PS00423 GRANINS_2
BLOCKS; BL00422
PFAM; PF01271 Granin
INTERPRO; IPR001819
Literature References
1. HUTTNER, W.B., GERDES, H.-H. AND ROSA, P.
The granin (chromogranin secretogranin) family.
TRENDS BIOCHEM.SCI. 16 27-30 (1991).
 
2. SIMON, J.-P. AND AUNIS, D.
Biochemistry of the chromogranin-A protein family.
BIOCHEM.J. 262 1-13 (1989).

Documentation
Chromogranins and secretogranins are acidic proteins present in the
secretory granules of endocrine and neuro-endocrine cells [1,2]. Granins 
may be precursors of biologically-active peptides, or they may be helper
proteins in the packaging of peptide hormones and neuropeptides - their
precise role is unclear. 
 
Similarity between these proteins is confined to short N- and C-terminal
regions: chromogranins A and B are characterised by an N-terminal motif
that includes 2 cysteine residues involved in disulphide bond formation;
and chromogranins and secretogranins together share a C-terminal motif.   
 
CHROMOGRANIN is a 3-element fingerprint that provides a signature for
chromogranins A and B. The fingerprint was derived from an initial alignment
of 8 sequences: motifs 1 and 2 were drawn from the N-terminus, and span
the region encoded by PROSITE pattern GRANINS_2 (PS00423), which includes
the conserved cysteine residues; and motif 3 lies at the C-terminus, and
spans the region encoded by PROSITE pattern GRANINS_1 (PS00422). Two
iterations on OWL29.1 were required to reach convergence, at which point
a true set comprising 14 sequences was identified.
 
An update on SPTR37_9f identified a true set of 10 sequences.
Summary Information
10 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3101010
2000
123
True Positives
CMGA_BOVIN    CMGA_HUMAN    CMGA_MOUSE    CMGA_RAT      
O02707 O35314 P79392 SG1_BOVIN
SG1_HUMAN SG1_MOUSE
Sequence Titles
CMGA_BOVIN  CHROMOGRANIN A PRECURSOR (CGA) (PITUITARY SECRETORY PROTEIN I) (SP-I) [CONTAINS: PANCREASTATIN; CHROMOSTATIN; WE-14; CATESTATIN] - BOS TAURUS (BOVINE). 
CMGA_HUMAN CHROMOGRANIN A PRECURSOR (CGA) (PITUITARY SECRETORY PROTEIN I) (SP-I) [CONTAINS: PANCREASTATIN; WE-14] - HOMO SAPIENS (HUMAN).
CMGA_MOUSE CHROMOGRANIN A PRECURSOR (CGA) [CONTAINS: PANCREASTATIN; BETA-GRANIN; WE-14] - MUS MUSCULUS (MOUSE).
CMGA_RAT CHROMOGRANIN A PRECURSOR (CGA) [CONTAINS: PANCREASTATIN; BETA-GRANIN; WE-14] - RATTUS NORVEGICUS (RAT).
O02707 CHROMOGRANIN B - BOS TAURUS (BOVINE).
O35314 CHROMOGRANIN B - RATTUS NORVEGICUS (RAT).
P79392 CHROMOGRANIN A - BOS TAURUS (BOVINE).
SG1_BOVIN SECRETOGRANIN I PRECURSOR (SGI) (CHROMOGRANIN B) [CONTAINS: SECRETOLYTIN] - BOS TAURUS (BOVINE).
SG1_HUMAN SECRETOGRANIN I PRECURSOR (SGI) (CHROMOGRANIN B) - HOMO SAPIENS (HUMAN).
SG1_MOUSE SECRETOGRANIN I PRECURSOR (SGI) (CHROMOGRANIN B) - MUS MUSCULUS (MOUSE).
Scan History
OWL29_0    2  100  NSINGLE    
SPTR37_9f 2 11 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
NKGDTEVMKCIVEVIS CMGA_HUMAN 26 26 -
NHNEEVVTHCIIEVLS SG1_BOVIN 27 27 -
TKGDTKVMKCVLEVIS CMGA_MOUSE 26 26 -
NKGDTKVMKCIVEVIS CMGA_STRCA 8 8 -
NKGDTEVMKCIVEVIS CMGA_PIG 24 24 -
TKGDTKVMKCVLEVIS CMGA_RAT 26 26 -
NKGDTEVMKCIVEVIS CMGA_BOVIN 26 26 -
NHNEGMVTRCIIEVLS SG1_HUMAN 27 27 -

Motif 2 width=16
Element Seqn Id St Int Rpt
SDTLSKPSPMPVSKEC CMGA_BOVIN 41 -1 -
SDTLSKPSPMPVSQEC CMGA_PIG 39 -1 -
SDTLSKPNPLPISEEC CMGA_STRCA 23 -1 -
SNALSKSSAPPITPEC SG1_HUMAN 42 -1 -
SDTLSKPSPMPVSQEC CMGA_HUMAN 41 -1 -
SNALLKSSAPPITPEC SG1_BOVIN 42 -1 -
SDSLSKPSPMPVSPEC CMGA_MOUSE 41 -1 -
SDSLSKPSPMPVSPEC CMGA_RAT 41 -1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
EDQELESLSAIEAELEKVA CMGA_MOUSE 436 379 -
EDQELESLSAIEAELEKVA CMGA_RAT 439 382 -
EDQELESLSAIEAELEKVA CMGA_BOVIN 422 365 -
EDQELESLAAIEAELESVA CMGA_STRCA 80 41 -
EDQELESLSAIEAELEKVA CMGA_PIG 419 364 -
EKKELENLAAMDLELQKIA SG1_HUMAN 652 594 -
EDQELESLSAIEAELEKVA CMGA_HUMAN 430 373 -
EEKELENLAAMDLELQKIA SG1_BOVIN 619 561 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
NKGDTEVMKCIVEVIS CMGA_BOVIN 26 26 -
NKGDTEVMKCIVEVIS P79392 26 26 -
NHNEGMVTRCIIEVLS SG1_HUMAN 27 27 -
NKGDTEVMKCIVEVIS CMGA_HUMAN 26 26 -
NHNEEVVTHCIIEVLS O02707 27 27 -
NHNEEVVTHCIIEVLS SG1_BOVIN 27 27 -
DHNEEMVTRCIIEVLS O35314 27 27 -
TKGDTKVMKCVLEVIS CMGA_MOUSE 26 26 -
TKGDTKVMKCVLEVIS CMGA_RAT 26 26 -
DHNEEMVTRCIIEVLS SG1_MOUSE 27 27 -

Motif 2 width=16
Element Seqn Id St Int Rpt
SDTLSKPSPMPVSKEC CMGA_BOVIN 41 -1 -
SDTLSKPSPMPVSKEC P79392 41 -1 -
SNALSKSSAPPITPEC SG1_HUMAN 42 -1 -
SDTLSKPSPMPVSQEC CMGA_HUMAN 41 -1 -
SNALLKSSAPPITPEC O02707 42 -1 -
SNALLKSSAPPITPEC SG1_BOVIN 42 -1 -
SNALSKSSAPTITPEC O35314 42 -1 -
SDSLSKPSPMPVSPEC CMGA_MOUSE 41 -1 -
SDSLSKPSPMPVSPEC CMGA_RAT 41 -1 -
SNALSKSSVPTITPEC SG1_MOUSE 42 -1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
EDQELESLSAIEAELEKVA CMGA_BOVIN 422 365 -
EDQELESLSAIEAELEKVA P79392 422 365 -
EKKELENLAAMDLELQKIA SG1_HUMAN 652 594 -
EDQELESLSAIEAELEKVA CMGA_HUMAN 430 373 -
EEKELENLAAMDLELQKIA O02707 619 561 -
EEKELENLAAMDLELQKIA SG1_BOVIN 619 561 -
EKKELENLAAMDLELQKIA O35314 650 592 -
EDQELESLSAIEAELEKVA CMGA_MOUSE 436 379 -
EDQELESLSAIEAELEKVA CMGA_RAT 439 382 -
EKKELENLAAMDLELQKIA SG1_MOUSE 652 594 -