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PR00650

Identifier
GPR12ORPHANR  [View Relations]  [View Alignment]  
Accession
PR00650
No. of Motifs
4
Creation Date
09-DEC-1996  (UPDATE 07-JUN-1999)
Title
GPR12 orphan receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00644 GPRORPHANR
INTERPRO; IPR000599
GCRDB; GCR_0288; GCR_0902
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. EIDNE, K.A., ZABAVNIK, J., PETERS, T., YOSHIDA, S., ANDERSON, L.
AND TAYLOR, P.L. 
Cloning, sequencing and tissue distribution of a candidate G protein-coupled
receptor from rat pituitary gland.
FEBS LETT. 292 243-248 (1991).
 
7. WATSON, S. AND ARKINSTALL,
Orphan receptors.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, pp223-230.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Several 7TM receptors have been cloned but their endogenous ligands are
unknown; these have been termed orphan receptors. GPR12 was isolated from
a rat pituitary library and is found in discrete regions of the brain,
pituitary and testis, but is absent in other tissues [6,7].
 
GPR12ORPHANR is a 4-element fingerprint that provides a signature for the
GPR12 orphan receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved sections
within either loop or N-terminal regions, focusing on those areas of the
alignment that characterise the GPR12 receptors but distinguish them from
the rest of the GPR family - motifs 1 and 2 span the N-terminus; motif 3
spans the C-terminus of TM domain 2 and the first external loop; and motif
4 lies in the third external loop. A single iteration on OWL29.0 was
required to reach convergence, no further sequences being identified beyond
the starting set. 
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
43333
30000
20000
1234
True Positives
GPRC_HUMAN    GPRC_MOUSE    GPRC_RAT      
Sequence Titles
GPRC_HUMAN  PROBABLE G PROTEIN-COUPLED RECEPTOR GPR12 - HOMO SAPIENS (HUMAN). 
GPRC_MOUSE PROBABLE G PROTEIN-COUPLED RECEPTOR GPR12 (GPCR01) - MUS MUSCULUS (MOUSE).
GPRC_RAT PROBABLE G PROTEIN-COUPLED RECEPTOR GPR12 (R334) - RATTUS NORVEGICUS (RAT).
Scan History
OWL29_0    1  30   NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
EDPKVNLSGLPRDCIDAGAPE GPRC_MOUSE 3 3 -
EDPKVNLSGLPRDCIEAGTPE GPRC_RAT 3 3 -
EDPKVNLSGLPRDCIEAGTPE RNCGPCR 3 3 -
EDLKVNLSGLPRDYLDAAAAE GPRC_HUMAN 3 3 -

Motif 2 width=17
Element Seqn Id St Int Rpt
ENISAAVPSQGSVVESE GPRC_RAT 23 -1 -
ENISAAVPSQGSVVESE RNCGPCR 23 -1 -
ENISAAVPSQGSVAESE GPRC_MOUSE 23 -1 -
ENISAAVSSRVPAVEPE GPRC_HUMAN 23 -1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
IINFVFAYLLQSEATK RNCGPCR 98 58 -
IINFVFAYLLQSEATK GPRC_MOUSE 98 58 -
IINFVFAYLLQSEATK GPRC_RAT 98 58 -
ITNFVFAYLLQSEATK GPRC_HUMAN 98 58 -

Motif 4 width=11
Element Seqn Id St Int Rpt
SLIADYTYPSI GPRC_RAT 271 157 -
SLIADYTYPSI GPRC_HUMAN 271 157 -
SLIADYTYPSI GPRC_MOUSE 271 157 -
SLIADYTYPSI RNCGPCR 270 156 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
EDPKVNLSGLPRDCIDAGAPE GPRC_MOUSE 3 3 -
EDPKVNLSGLPRDCIEAGTPE GPRC_RAT 3 3 -
EDLKVNLSGLPRDYLDAAAAE GPRC_HUMAN 3 3 -

Motif 2 width=17
Element Seqn Id St Int Rpt
ENISAAVPSQGSVAESE GPRC_MOUSE 23 -1 -
ENISAAVPSQGSVVESE GPRC_RAT 23 -1 -
ENISAAVSSRVPAVEPE GPRC_HUMAN 23 -1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
IINFVFAYLLQSEATK GPRC_MOUSE 98 58 -
IINFVFAYLLQSEATK GPRC_RAT 98 58 -
ITNFVFAYLLQSEATK GPRC_HUMAN 98 58 -

Motif 4 width=11
Element Seqn Id St Int Rpt
SLIADYTYPSI GPRC_MOUSE 271 157 -
SLIADYTYPSI GPRC_RAT 271 157 -
SLIADYTYPSI GPRC_HUMAN 271 157 -