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PR00614

Identifier
NIHGNASESMLL  [View Relations]  [View Alignment]  
Accession
PR00614
No. of Motifs
11
Creation Date
16-SEP-1996  (UPDATE 10-JUN-1999)
Title
Ni-Fe hydrogenase small subunit signature
Database References

INTERPRO; IPR001821
Literature References
1. FAUQUE, G., PECK, H.D., MOURA, J.J.G., HUYNH, B.H., BERLIER, Y.,
DERVARTANIAN, D.V., TEIXEIRA, M., PRZYBYLA, A.E., LESPINAT, P.A., MOURA, I.
AND LEGALL, J. 
The three classes of hydrogenases from sulfate-reducing bacteria of the
genus Desulfovibrio.
FEMS MICROBIOL.REV. 4 299-344 (1988). 
 
2. CAMMACK, R., FERNANDEZ, V.M. AND HATCHIKIAN, E.C.
Nickel-iron hydrogenase.
METHODS ENZYMOL. 243 43-68 (1994). 
 
3. PRZYBYLA, A.E., ROBBINS, J., MENON, N. AND PECK, H.D.
Structure-function relationships among the nickel-containing hydrogenases.
FEMS MICROBIOL.REV. 8 109-135 (1992). 
 
4. VOLBEDA, A., CHARON, M.H., PIRAS, C., HATCHIKIAN, E.C., FREY, M.
AND FONTECILLA-CAMPS, J.C.
Crystal structure of the nickel-iron hydrogenase from Desulfovibrio gigas.
NATURE 373 580-587 (1995).

Documentation
Hydrogenases catalyse the reversible oxidation of molecular hydrogen and 
play a vital role in anaerobic metabolism. Metal-containing hydrogenases 
are subdivided into three classes: Fe (`iron only') hydrogenases; Ni-Fe 
hydrogenases; and Ni-Fe-Se hydrogenases [1]. Hydrogen oxidation is coupled 
to the reduction of electron acceptors (such as oxygen, nitrate, sulphate, 
carbon dioxide and fumarate), whereas proton reduction (hydrogen evolution)
is essential in pyruvate fermentation or in the disposal of excess electrons. 
 
The Ni-Fe hydrogenases, when isolated, are found to catalyse both hydrogen
evolution and uptake, with low-potential multihaem cytochromes, such as 
cytochrome c3, acting as either electron donors or acceptors, depending on 
their oxidation state [2]. Both periplasmic (soluble) and membrane-bound
hydrogenases are known.
 
The Ni-Fe hydrogenases are heterodimeric proteins consisting of small (S) 
and large (L) subunits. The small subunit contains three iron-sulphur
clusters (two [4Fe-4S] and one [3Fe-4S]); the large subunit contains a 
nickel ion [2,3]. Small subunits of membrane-bound Ni-Fe hydrogenases
contain a C-terminal domain of about 40 residues that is absent in
periplasmic forms.
 
The 3D structure of the Ni-Fe hydrogenase from Desulfovibrio gigas has been
determined at 2.85A resolution [4]. The small subunit consists of two 
domains, I(S) and II(S). The alpha/beta twisted open sheet structure of the
N-terminal I(S) domain is similar to that of flavodoxin; the C-terminal
II(S) domain contains two alpha-helices and has no beta-structure. The Fe-S 
clusters are distributed almost along a straight line, with the [3Fe-4S]
cluster located half-way between the two [4Fe-4S] clusters. The two [4Fe-4S] 
clusters have been termed proximal (prox) and distal (dist), based on their
distance to the Ni atom. Domain I(S) binds the [4Fe-4S]prox cluster, while
domain II(S) binds the [4Fe-4S]dist and [3Fe-4S] clusters. The [4Fe-4S]prox 
cluster is coordinated by Cys-17, Cys-20, Cys-112 and Cys-148; [4Fe-4S]dist 
is coordinated by His-185, Cys-188, Cys-213 and Cys-219; and [3Fe-4S] is
coordinated by Cys-228, Cys-246 and Cys-249. [4Fe-4S]dist is the first known
example of a [4Fe-4S] cluster in protein structure ligated by a His side
chain. A crown of acidic residues surrounds the partially-exposed His-185
and this might provide a recognition site for the redox partner (cytochrome
c3) [4]. A mechanism of electron transfer from the Ni active site through
the Fe-S clusters to the cytochrome c3 has been suggested [4]. The role of
the [3Fe-4S] cluster is not clear: its high redox potential and its absence
from some homologous hydrogenases put its involvement in electron transfer
in doubt [4]. 
 
NIHGNASESMLL is an 11-element fingerprint that provides a signature for the
small subunit of Ni-Fe hydrogenases. The fingerprint was derived from an 
initial alignment of 12 sequences: the motifs were drawn from conserved 
regions spanning virtually the full alignment length - motifs 1-6 span the 
I(S) domain and motifs 7-11 span the II(S) domain. Motif 1 includes two
invariant Cys residues that bind [4Fe-4S]prox; motifs 5 and 6 each include
a conserved Cys, which contribute to the [4Fe-4S]prox cluster; motif 8
includes the conserved His and Cys residues that bind [4Fe-4S]dist; motif 9
includes three invariant Cys residues, the first two of which bind
[4Fe-4S]dist, the third binding the [3Fe-4S] cluster; and motif 10 contains
two conserved Cys residues that contribute to the [3Fe-4S] cluster. Two
iterations on OWL28.1 were required to reach convergence, at which point a
true set comprising 21 sequences was identified. Several partial matches
were also found: PHSS_DESBA (which lacks motif 10) is a Ni-Fe-Se hydrogenase
containing only two Fe-S centres; ECU2837794 is an E.coli hydrogenase that
lacks motif 11; MMDNAVHT (lacking motifs 2, 5 and 11) and S49928 (lacking
motifs 2, 5, 7 and 11) are membrane-bound hydrogenases from Methanosarcina
mazei; DVUPNFHGA (lacking motifs 5-8), is a periplasmic Ni-Fe hydrogenase
from Desulfovibrio gigas; RCAHUPT1 (matching motifs 5-7 and 9), B49938
(matching motifs 5, 6 and 8) and RHBHUPUVA (matching 1, 5 and 6) are hupU
gene products; FRHG_METTH (matching motifs motifs 1, 4 and 6) is the gamma-
subunit of the nickel-containing 8-hydroxy-5-deazaflavin reducing
hydrogenase from Methanobacterium thermoautotrophicum; S24800, C30315,
D30315 and MBFMVRH2 (all matching only motifs 4 and 6), are methyl
viologen-reducing hydrogenase gamma-subunits.
 
An update on SPTR37_9f identified a true set of 22 sequences, and 12
partial matches.
Summary Information
  22 codes involving 11 elements
0 codes involving 10 elements
2 codes involving 9 elements
0 codes involving 8 elements
2 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
2 codes involving 4 elements
3 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
112222222222222222222222
1000000000000
921112222212
800000000000
720220202220
600000000000
500011100110
400001210220
330003100200
200000000000
1234567891011
True Positives
MBHS_ALCEU    MBHS_ALCHY    MBHS_AZOCH    MBHS_AZOVI    
MBHS_BRAJA MBHS_CITFR MBHS_ECOLI MBHS_RHILV
MBHS_RHOCA MBHS_RHOGE MBHS_WOLSU O25348
O33405 O66894 O67095 O86467
PHNS_DESDE PHNS_DESFR PHNS_DESGI PHNS_DESVM
Q51860 Q56359
True Positive Partials
Codes involving 9 elements
O28890 PHSS_DESBA
Codes involving 7 elements
Q50225 Q50248
Codes involving 5 elements
O68306
Codes involving 4 elements
O66987 Q44215
Codes involving 3 elements
P94154 P95603 Q45254
Codes involving 0 elements
MBHT_ECOLI O51820
Sequence Titles
MBHS_ALCEU  UPTAKE HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (HYDROGENLYASE) (MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT) - ALCALIGENES EUTROPHUS. 
MBHS_ALCHY UPTAKE HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (HYDROGENLYASE) (MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT) - ALCALIGENES HYDROGENOPHILUS.
MBHS_AZOCH UPTAKE HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (HYDROGENLYASE) (MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT) - AZOTOBACTER CHROOCOCCUM MCD 1.
MBHS_AZOVI UPTAKE HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (HYDROGENLYASE) (MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT) (HYDROGENASE BETA SUBUNIT) - AZOTOBACTER VINELANDII.
MBHS_BRAJA UPTAKE HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (HYDROGENLYASE) (MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT) - BRADYRHIZOBIUM JAPONICUM.
MBHS_CITFR HYDROGENASE-1 SMALL CHAIN PRECURSOR (EC 1.18.99.1) (NIFE HYDROGENASE) (MEMBRANE-BOUND HYDROGENASE 1 SMALL SUBUNIT) (HYD1) - CITROBACTER FREUNDII.
MBHS_ECOLI HYDROGENASE-1 SMALL CHAIN PRECURSOR (EC 1.18.99.1) (NIFE HYDROGENASE) (MEMBRANE-BOUND HYDROGENASE 1 SMALL SUBUNIT) (HYD1) - ESCHERICHIA COLI.
MBHS_RHILV UPTAKE HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (HYDROGENLYASE) (MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT) - RHIZOBIUM LEGUMINOSARUM (BIOVAR VICIAE).
MBHS_RHOCA UPTAKE HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (HYDROGENLYASE) (MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT) - RHODOBACTER CAPSULATUS (RHODOPSEUDOMONAS CAPSULATA).
MBHS_RHOGE UPTAKE HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (HYDROGENLYASE) (MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT) - RHODOCYCLUS GELATINOSUS (RHODOPSEUDOMONAS GELATINOSA).
MBHS_WOLSU QUINONE-REACTIVE NI/FE-HYDROGENASE SMALL CHAIN PRECURSOR (EC 1.12.99.3) (MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT) (HYDROGEN:QUINONE OXIDOREDUCTASE) - WOLINELLA SUCCINOGENES.
O25348 QUINONE-REACTIVE NI/FE HYDROGENASE, SMALL SUBUNIT (HYDA) - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
O33405 UPTAKE HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (HYDROGENLYASE) (MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT) (HOXS) - PSEUDOMONAS CARBOXYDOVORANS.
O66894 HYDROGENASE SMALL SUBUNIT - AQUIFEX AEOLICUS.
O67095 HYDROGENASE SMALL SUBUNIT - AQUIFEX AEOLICUS.
O86467 UPTAKE HYDROGENASE - RHODOBACTER SPHAEROIDES (RHODOPSEUDOMONAS SPHAEROIDES).
PHNS_DESDE PERIPLASMIC [NIFE] HYDROGENASE SMALL SUBUNIT (EC 1.18.99.1) (NIFE HYDROGENLYASE SMALL CHAIN) - DESULFOVIBRIO DESULFURICANS.
PHNS_DESFR PERIPLASMIC [NIFE] HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (NIFE HYDROGENLYASE SMALL CHAIN) - DESULFOVIBRIO FRUCTOSOVORANS.
PHNS_DESGI PERIPLASMIC [NIFE] HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (NIFE HYDROGENLYASE SMALL CHAIN) - DESULFOVIBRIO GIGAS.
PHNS_DESVM PERIPLASMIC [NIFE] HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (NIFE HYDROGENLYASE SMALL CHAIN) - DESULFOVIBRIO VULGARIS (STRAIN MIYAZAKI).
Q51860 MEMBRANE-BOUND HYDROGENASE SMALL SUBUNIT PRECURSOR - PSEUDOMONAS HYDROGENOVORA.
Q56359 HYDROGENASE SMALL SUBUNIT - THIOCAPSA ROSEOPERSICINA.

O28890 F420-NONREDUCING HYDROGENASE (VHTG) - ARCHAEOGLOBUS FULGIDUS.
PHSS_DESBA PERIPLASMIC [NIFESE] HYDROGENASE SMALL SUBUNIT PRECURSOR (EC 1.18.99.1) (NIFESE HYDROGENLYASE SMALL CHAIN) - DESULFOVIBRIO BACULATUS (DESULFOMICROBIUM BACULATUS).

Q50225 F420-NONREDUCING HYDROGENASE PRECURSOR (EC 1.12.99.) - METHANOSARCINA MAZEI.
Q50248 F420-NONREDUCING HYDROGENASE I PRECURSOR - METHANOSARCINA MAZEI.

O68306 HUPS HOMOLOG - NOSTOC PCC73102.

O66987 HYDROGENASE SMALL SUBUNIT - AQUIFEX AEOLICUS.
Q44215 [NIFE] UPTAKE HYDROGENASE SMALL SUBUNIT - ANABAENA SP. (STRAIN PCC 7120).

P94154 [NIFE] HYDROGENASE-LIKE PROTEIN SMALL SUBUNIT - ALCALIGENES HYDROGENOPHILUS.
P95603 [NIFE] HYDROGENASE-LIKE PROTEIN SMALL SUBUNIT - ALCALIGENES EUTROPHUS.
Q45254 HUPU AND HUPV GENES, COMPLETE CDS - BRADYRHIZOBIUM JAPONICUM.

MBHT_ECOLI HYDROGENASE-2 SMALL CHAIN PRECURSOR (EC 1.18.99.1) (NIFE HYDROGENASE) (MEMBRANE-BOUND HYDROGENASE 2 SMALL SUBUNIT) (HYD2) - ESCHERICHIA COLI.
O51820 STABLE NIFE HYDROGENASE SMALL SUBUNIT PRECURSOR - THIOCAPSA ROSEOPERSICINA.
Scan History
OWL28_1    2  270  NSINGLE    
SPTR37_9f 2 104 NSINGLE
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
ETKPRTPVLWLHGLECTCCSESF MBHS_AZOVI 47 47 -
ETKPRTPVLWLHGLECTCCSESF MBHS_ALCHY 45 45 -
ETRPPTPVVWLHGLECTCCSESF MBHS_AZOCH 36 36 -
ETKPRVPVIWMHGLECTCCSESF MBHS_BRAJA 48 48 -
ETKPRTPVVWVHGLECTCCSESF MBHS_RHOCA 47 47 -
ETKERVPVIWMHGLECTCCSESF MBHS_RHILV 47 47 -
ETKPRTPVLWLHGLECTCCSESF MBHS_RHOGE 44 44 -
ENKPRIPVVWIHGLECTCCTESF MBHS_ECOLI 47 47 -
ELADRLPVIWLHMAECTGCSESL MBHS_WOLSU 38 38 -
TAKKRPSVVYLHNAECTGCSESL PHNS_DESGI 26 26 -
TAKHRPSVVWLHNAECTGCTEAA PHNS_DESFR 26 26 -
MGPRRPSVVYLHNAECTGCSESV PHNS_DESVM 52 52 -

Motif 2 width=25
Element Seqn Id St Int Rpt
IRSAHPLVKDVVLSMISLDYDDTLM MBHS_AZOVI 70 0 -
IRSAHPLAKDVVLSMISLDYDDTLM MBHS_ALCHY 68 0 -
IRSGDPLVKDVVLSMISLDYDDTLM MBHS_AZOCH 59 0 -
IRSAHPLVKDAVLSMISLDYDDTIM MBHS_BRAJA 71 0 -
IRSAHPLAKDVVLSMISLDYDDTLM MBHS_RHOCA 70 0 -
IRSAHPLVKDVVLSMISLDYDDTIM MBHS_RHILV 70 0 -
IRSAHPLAKDAVLSMISLDYDDTLM MBHS_RHOGE 67 0 -
IRSAHPLAKDVILSLISLDYDDTLM MBHS_ECOLI 70 0 -
LRTDGPGIDSLIFDYISLEYHETVM MBHS_WOLSU 61 0 -
LRTVDPYVDELILDVISMDYHETLM PHNS_DESGI 49 0 -
IRTIKPYIDALILDTISLDYQETIM PHNS_DESFR 49 0 -
LRAFEPYIDTLILDTLSLDYHETIM PHNS_DESVM 75 0 -

Motif 3 width=24
Element Seqn Id St Int Rpt
YILAVEGNPPLNEDGMFCIVGGKP MBHS_AZOVI 116 21 -
YILAVEGNPPLNQDGMSCIIGGKP MBHS_ALCHY 114 21 -
YILAVEGNPPLNEDGMFCIVGGKP MBHS_AZOCH 102 18 -
YILAVEGNPPLNEGGMFCIDGGKP MBHS_BRAJA 117 21 -
YILAVEGNPPLNEDGMFCITGGKP MBHS_RHOCA 116 21 -
YILAVEGNPPLNEGGMFCIDGGKP MBHS_RHILV 116 21 -
YILAVEGNPPLNEDGMFCIQRQAL MBHS_RHOGE 113 21 -
YILAVEGNPPLGEQGMFCISSGRP MBHS_ECOLI 116 21 -
YVLMVEGGIPAGSSEFYLTVGPHG MBHS_WOLSU 107 21 -
FVCVIEGGIPMGDGGYWGKVGRRN PHNS_DESGI 92 18 -
YYLVVEGGLPTIDGGQWGMVAGHP PHNS_DESFR 94 20 -
FIAVVEGGIPTAANGIYGKVANHT PHNS_DESVM 120 20 -

Motif 4 width=15
Element Seqn Id St Int Rpt
LRHVAKDAKAVIAWG MBHS_AZOVI 144 4 -
LRHVAKDAKAIISWG MBHS_ALCHY 142 4 -
LKHAAKDAKAVIAWG MBHS_AZOCH 130 4 -
LKMMAEDAMAIIAWG MBHS_BRAJA 145 4 -
LRHAAEGAKAIISWG MBHS_RHOCA 144 4 -
LKWMAEDAMAIIAWG MBHS_RHILV 144 4 -
AQAVAADCKAVIAWG MBHS_RHOGE 140 3 -
LKRAAAGASAIIAWG MBHS_ECOLI 144 4 -
ARHASANAAAIFAIG MBHS_WOLSU 137 6 -
CAEVAPKAKAVIAIG PHNS_DESGI 120 4 -
TKKAAAKAKGIICIR PHNS_DESFR 122 4 -
CSRILPKAQAVIAYG PHNS_DESVM 148 4 -

Motif 5 width=25
Element Seqn Id St Int Rpt
SCASWGCVQAARPNPTQAVPIHKVI MBHS_AZOVI 159 0 -
SCASWGCVQAAKANPTQATPIHKVI MBHS_ALCHY 157 0 -
SCASWGCVQAAKPNPTQAVPIHKVI MBHS_AZOCH 145 0 -
ACASWGCVQAAKPNPTQATPIDKVI MBHS_BRAJA 160 0 -
ACASYGCVQAAAPNPTQATPVHKVI MBHS_RHOCA 159 0 -
ASASWGCVQAAKPNPTQATPIDKVI MBHS_RHILV 159 0 -
SCASWGCVQAAKPNPTQATPIHKVI MBHS_RHOGE 155 0 -
TCASWGCVQAARPNPTQATPIDKVI MBHS_ECOLI 159 0 -
SCSSFGGVQAARPNPTNAQPLSKVT MBHS_WOLSU 152 0 -
TCATYGGVQAAKPNPTGTVGVNEAL PHNS_DESGI 135 0 -
RHLPHGGVQKAKPNPSQAKGVSEAL PHNS_DESFR 136 -1 -
TCATFGGVQAAKPNPTGAKGVNDAL PHNS_DESVM 163 0 -

Motif 6 width=24
Element Seqn Id St Int Rpt
IVKVPGCPPIAEVMTGVITYMLTF MBHS_AZOVI 188 4 -
IIKVPGCPPIAEVMTGVITYMLTF MBHS_ALCHY 186 4 -
MIKVPGCPPIAEVMTGVITYMLTF MBHS_AZOCH 174 4 -
IIKVPGCPPIAEVMTGVVTFITTF MBHS_BRAJA 189 4 -
IIKVPGCPPIAEVMTGVITYMLTF MBHS_RHOCA 188 4 -
IIKVPGCPPIAEVMTGVVTFITTF MBHS_RHILV 188 4 -
IIKVPGCPPIAEVMTGVITYMLTF MBHS_RHOGE 184 4 -
IIKVPGCPPIPDVMSAIITYMVTF MBHS_ECOLI 188 4 -
VINVPGCPPSEKNIVGNVLHFILF MBHS_WOLSU 180 3 -
AINIAGCPPNPMNFVGTVVHLLTK PHNS_DESGI 166 6 -
TINIPGCPPNPINFVGAVVHVLTK PHNS_DESFR 164 3 -
AINIAGCPPNPYNLVGTIVYYLKN PHNS_DESVM 194 6 -

Motif 7 width=18
Element Seqn Id St Int Rpt
LPELDRQGRPKMFYGQRI MBHS_AZOVI 214 2 -
FPELDRQGRPKMFYSQRI MBHS_ALCHY 212 2 -
LPELDRQGRPKMFYGQRI MBHS_AZOCH 200 2 -
LPELDRQGRPKMFYSQRI MBHS_BRAJA 215 2 -
MPELDRQGRPAMFYSQRI MBHS_RHOCA 214 2 -
LPELDRQGPPKMFYAQPI MBHS_RHILV 214 2 -
IPELDRQGRPKMFYSQRI MBHS_RHOGE 210 2 -
LPDVDRMGRPLMFYGQRI MBHS_ECOLI 214 2 -
LPSVDAFNRPMWAYGLRI MBHS_WOLSU 206 2 -
MPELDKQGRPVMFFGETV PHNS_DESGI 191 1 -
IPDLDENGRPKLFYGELV PHNS_DESFR 189 1 -
APELDSLNRPTMFFGQTV PHNS_DESVM 220 2 -

Motif 8 width=19
Element Seqn Id St Int Rpt
HDKCYRRPHFDAGQFVEHW MBHS_AZOVI 232 0 -
HDKCYRRPHFDAGQFVESW MBHS_ALCHY 230 0 -
HDKSYRRPHFDAGQFVEHW MBHS_AZOCH 218 0 -
HDKCYRRPHFDAGQFVEEW MBHS_BRAJA 233 0 -
HDKCYRRPHFDAGQFVEHW MBHS_RHOCA 232 0 -
HDKCYRRPHFDAGQFVEEW MBHS_RHILV 232 0 -
HDKCYRRPHFDAGQFVESF MBHS_RHOGE 228 0 -
HDKCYRRAHFDAGEFVQSW MBHS_ECOLI 232 0 -
HDLCERRGRFDAGEFVQEF MBHS_WOLSU 224 0 -
HDNCPRLKHFEAGEFATSF PHNS_DESGI 209 0 -
HDNCPRLPHFEASEFAPSF PHNS_DESFR 207 0 -
HEQCPRLPHFDAGEFAPSF PHNS_DESVM 238 0 -

Motif 9 width=27
Element Seqn Id St Int Rpt
RKGYCLYKVGCKGPTSYNACSTVRWNE MBHS_AZOVI 256 5 -
RKGYCLYKVGCKGPTTYNACSTTRWNG MBHS_ALCHY 254 5 -
RKGYCLYKVGCKGPTSYNACSTVRWNE MBHS_AZOCH 242 5 -
RKGYCLYKMGCKGPTTYNACSTVRWNG MBHS_BRAJA 257 5 -
RKGYCLYKMGCKGPTTYNACSTVPLER MBHS_RHOCA 256 5 -
RKGYCLYKMGCKGPTTYNACSTVRWNG MBHS_RHILV 256 5 -
RKGFCLYKVGCKGPTTYNACSTVMWNE MBHS_RHOGE 252 5 -
RKGYCLYKMGCKGPTTYNACSSTRWND MBHS_ECOLI 256 5 -
KKGYCLYKVGCKGPYTFNNCSKLRFNQ MBHS_WOLSU 248 5 -
KKGYCLYELGCKGPDTYNNCPKQLFNQ PHNS_DESGI 233 5 -
KKGFCLYELGCKGPVTYNNCPKVLFNQ PHNS_DESFR 231 5 -
RKGWCLYELGCKGPVTMNNCPKIKFNQ PHNS_DESVM 262 5 -

Motif 10 width=14
Element Seqn Id St Int Rpt
TSFPIQAGHGCIGC MBHS_AZOVI 284 1 -
TSFPIQSGHGCIGC MBHS_ALCHY 282 1 -
TSFPIQAGHGCIGC MBHS_AZOCH 270 1 -
VSFPIQSGHGCIGC MBHS_BRAJA 285 1 -
RHFPIQSGHGCIGC MBHS_RHOCA 284 1 -
VSFPIQSGHGCIGC MBHS_RHILV 284 1 -
TSFPIKAGHGARRS MBHS_RHOGE 280 1 -
VSFPIQSGHGCLGC MBHS_ECOLI 284 1 -
TSWPVQAGHGCIGC MBHS_WOLSU 276 1 -
VNWPVQAGHPCIAC PHNS_DESGI 260 0 -
VNWPVQAGHPCLGC PHNS_DESFR 258 0 -
TNWPVDAGHPCIGC PHNS_DESVM 289 0 -

Motif 11 width=13
Element Seqn Id St Int Rpt
SEDGFWDKGSFYE MBHS_AZOVI 298 0 -
SEDGFWDKGSFYS MBHS_ALCHY 296 0 -
SEDGFWDKGSFYE MBHS_AZOCH 284 0 -
SEDGFWDKGSFYD MBHS_BRAJA 299 0 -
SEDGFWDQGSFYD MBHS_RHOCA 298 0 -
SEDGFWDNGSFYD MBHS_RHILV 298 0 -
SEDGFWDKGSFYD MBHS_RHOGE 293 -1 -
AENGFWDRGSFYS MBHS_ECOLI 298 0 -
SEPDFWDTMGPFE MBHS_WOLSU 290 0 -
SEPNFWDLYSPFY PHNS_DESGI 274 0 -
SEPDFWDTMTPFY PHNS_DESFR 272 0 -
SEPDFWDAMTPFY PHNS_DESVM 303 0 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
ETKPRTPVLWLHGLECTCCSESF MBHS_ALCEU 45 45 -
ETKPRTPVLWLHGLECTCCSESF MBHS_AZOVI 47 47 -
ETKPRTPVLWLHGLECTCCSESF MBHS_ALCHY 45 45 -
ETKPRTPVLWRHGLECTCCSESF Q51860 45 45 -
ETRPPTPVVWLHGLECTCCSESF MBHS_AZOCH 36 36 -
ETKPRTPVIWVHGLECTCCSESF O86467 47 47 -
ETKPRVPVIWMHGLECTCCSESF MBHS_BRAJA 48 48 -
ETKPRTPVVWVHGLECTCCSESF MBHS_RHOCA 47 47 -
ETKPRTPVLWLHGLECTCCSESF O33405 47 47 -
ETKERVPVIWMHGLECTCCSESF MBHS_RHILV 47 47 -
ETKPRIPVVWLHGLECTCCSESF Q56359 47 47 -
ETKPRTPVLWLHGLECTCCSESF MBHS_RHOGE 44 44 -
ENKPRIPVVWIHGLECTCCTESF MBHS_CITFR 49 49 -
ENKPRIPVVWIHGLECTCCTESF MBHS_ECOLI 47 47 -
ETKPRVPVLWIHGLECTCCSESF O66894 44 44 -
EVANRLPVIWLHMAECTGCSESL O25348 71 71 -
EKKEKPVVIWLEFQDCAGCSESF O67095 44 44 -
ELADRLPVIWLHMAECTGCSESL MBHS_WOLSU 38 38 -
TAKKRPSVVYLHNAECTGCSESL PHNS_DESGI 26 26 -
TAKHRPSVVWLHNAECTGCTEAA PHNS_DESFR 26 26 -
MGPRRPSVVYLHNAECTGCSESV PHNS_DESVM 52 52 -
LTGRRPSVVYLHAAECTGCSEAL PHNS_DESDE 2 2 -

Motif 2 width=25
Element Seqn Id St Int Rpt
IRSAHPLAKDVVLSMISLDYDDTLM MBHS_ALCEU 68 0 -
IRSAHPLVKDVVLSMISLDYDDTLM MBHS_AZOVI 70 0 -
IRSAHPLAKDVVLSMISLDYDDTLM MBHS_ALCHY 68 0 -
IRSAHPLAKDVVLSMISLDYDDTLI Q51860 68 0 -
IRSGDPLVKDVVLSMISLDYDDTLM MBHS_AZOCH 59 0 -
IRAAHPLAKDVVLSMISLDYDDTLM O86467 70 0 -
IRSAHPLVKDAVLSMISLDYDDTIM MBHS_BRAJA 71 0 -
IRSAHPLAKDVVLSMISLDYDDTLM MBHS_RHOCA 70 0 -
IPSAHPLVKDVVLSMISLDYDDTLM O33405 70 0 -
IRSAHPLVKDVVLSMISLDYDDTIM MBHS_RHILV 70 0 -
IRSAHPLVSDVILSMISLDYTILIM Q56359 70 0 -
IRSAHPLAKDAVLSMISLDYDDTLM MBHS_RHOGE 67 0 -
IRSSHPLAKDVILSLISLDYDDTLM MBHS_CITFR 72 0 -
IRSAHPLAKDVILSLISLDYDDTLM MBHS_ECOLI 70 0 -
IRSATPLASDVVLSMISLEYDDTLS O66894 67 0 -
LRSADPTIDSIIFDYINLEYHETIM O25348 94 0 -
IRSTTLLPTEVLLDYISLEYHETLM O67095 67 0 -
LRTDGPGIDSLIFDYISLEYHETVM MBHS_WOLSU 61 0 -
LRTVDPYVDELILDVISMDYHETLM PHNS_DESGI 49 0 -
IRTIKPYIDALILDTISLDYQETIM PHNS_DESFR 49 0 -
LRAFEPYIDTLILDTLSLDYHETIM PHNS_DESVM 75 0 -
LRTYQPFIDTLILDTISLDYHETIM PHNS_DESDE 25 0 -

Motif 3 width=24
Element Seqn Id St Int Rpt
YILAVEGNPPLNQDGMSCIIGGRP MBHS_ALCEU 114 21 -
YILAVEGNPPLNEDGMFCIVGGKP MBHS_AZOVI 116 21 -
YILAVEGNPPLNQDGMSCIIGGKP MBHS_ALCHY 114 21 -
YILAVEGNPPLNQDGMSCIIRGRP Q51860 114 21 -
YILAVEGNPPLNEDGMFCIVGGKP MBHS_AZOCH 102 18 -
YILAVEGNPPLNEDGMYCIIGGKP O86467 116 21 -
YILAVEGNPPLNEGGMFCIDGGKP MBHS_BRAJA 117 21 -
YILAVEGNPPLNEDGMFCITGGKP MBHS_RHOCA 116 21 -
YILAVEGNPPLNEDGMFCIIGGKP O33405 116 21 -
YILAVEGNPPLNEGGMFCIDGGKP MBHS_RHILV 116 21 -
YILAVEGNPPLNQDGMSCIIGGRP Q56359 116 21 -
YILAVEGNPPLNEDGMFCIQRQAL MBHS_RHOGE 113 21 -
YILAVEGNPPLGEQGMFCISGGRP MBHS_CITFR 118 21 -
YILAVEGNPPLGEQGMFCISSGRP MBHS_ECOLI 116 21 -
YILAVEGNPPLGEDGMYCIIGGRP O66894 113 21 -
YILMVEGGIPQGTEYFLTQGPNAE O25348 140 21 -
YVLVVEGSPTPADGGVYCTVGGKS O67095 113 21 -
YVLMVEGGIPAGSSEFYLTVGPHG MBHS_WOLSU 107 21 -
FVCVIEGGIPMGDGGYWGKVGRRN PHNS_DESGI 92 18 -
YYLVVEGGLPTIDGGQWGMVAGHP PHNS_DESFR 94 20 -
FIAVVEGGIPTAANGIYGKVANHT PHNS_DESVM 120 20 -
FICLVEGAIPTGMDNKYGYIAGHT PHNS_DESDE 70 20 -

Motif 4 width=15
Element Seqn Id St Int Rpt
LKYVAKDAKAIISWG MBHS_ALCEU 142 4 -
LRHVAKDAKAVIAWG MBHS_AZOVI 144 4 -
LRHVAKDAKAIISWG MBHS_ALCHY 142 4 -
LKRVSADAKAIISWG Q51860 142 4 -
LKHAAKDAKAVIAWG MBHS_AZOCH 130 4 -
LKMAAEHAKAIISWG O86467 144 4 -
LKMMAEDAMAIIAWG MBHS_BRAJA 145 4 -
LRHAAEGAKAIISWG MBHS_RHOCA 144 4 -
LRYAAKHAKAIISWG O33405 144 4 -
LKWMAEDAMAIIAWG MBHS_RHILV 144 4 -
LLEMADSCKAVISWG Q56359 144 4 -
AQAVAADCKAVIAWG MBHS_RHOGE 140 3 -
AKRAAAGASAIIAWG MBHS_CITFR 146 4 -
LKRAAAGASAIIAWG MBHS_ECOLI 144 4 -
LKESAEGAKAVIAWG O66894 141 4 -
CRKAAQYAAAIFAIG O25348 169 5 -
LRETAEHAAFVIAVG O67095 141 4 -
ARHASANAAAIFAIG MBHS_WOLSU 137 6 -
CAEVAPKAKAVIAIG PHNS_DESGI 120 4 -
TKKAAAKAKGIICIR PHNS_DESFR 122 4 -
CSRILPKAQAVIAYG PHNS_DESVM 148 4 -
CKNILPKAKAVVSIG PHNS_DESDE 98 4 -

Motif 5 width=25
Element Seqn Id St Int Rpt
SCASWGCVQAAKPNPTQATPVHKVI MBHS_ALCEU 157 0 -
SCASWGCVQAARPNPTQAVPIHKVI MBHS_AZOVI 159 0 -
SCASWGCVQAAKANPTQATPIHKVI MBHS_ALCHY 157 0 -
SCASWGCVQAAKPNPTQATPVHKVI Q51860 157 0 -
SCASWGCVQAAKPNPTQAVPIHKVI MBHS_AZOCH 145 0 -
ACASYGCVQAAAPNPTRATPVHKVI O86467 159 0 -
ACASWGCVQAAKPNPTQATPIDKVI MBHS_BRAJA 160 0 -
ACASYGCVQAAAPNPTQATPVHKVI MBHS_RHOCA 159 0 -
SCASHGCVQAARPNPTRATPVHQVI O33405 159 0 -
ASASWGCVQAAKPNPTQATPIDKVI MBHS_RHILV 159 0 -
SCASWGCVQAARPNPTRATPVHEVI Q56359 159 0 -
SCASWGCVQAAKPNPTQATPIHKVI MBHS_RHOGE 155 0 -
TCASWGCVQAARPNPTQATPIDKVI MBHS_CITFR 161 0 -
TCASWGCVQAARPNPTQATPIDKVI MBHS_ECOLI 159 0 -
SCASWGCVQAAKPNPTTAVPIDKVI O66894 156 0 -
TCSSFGGVQAAYPNPSNAQPLHKII O25348 184 0 -
SCASWGGIPKASPNPTGAVPVYEIV O67095 156 0 -
SCSSFGGVQAARPNPTNAQPLSKVT MBHS_WOLSU 152 0 -
TCATYGGVQAAKPNPTGTVGVNEAL PHNS_DESGI 135 0 -
RHLPHGGVQKAKPNPSQAKGVSEAL PHNS_DESFR 136 -1 -
TCATFGGVQAAKPNPTGAKGVNDAL PHNS_DESVM 163 0 -
TCASYGGIQAAKPNPNPTAAKGIND PHNS_DESDE 113 0 -

Motif 6 width=24
Element Seqn Id St Int Rpt
IIKVPGCPPIAEVMTGVITYMLTF MBHS_ALCEU 186 4 -
IVKVPGCPPIAEVMTGVITYMLTF MBHS_AZOVI 188 4 -
IIKVPGCPPIAEVMTGVITYMLTF MBHS_ALCHY 186 4 -
IIKVPGCPPIAEVMTGVITYMLTF Q51860 186 4 -
MIKVPGCPPIAEVMTGVITYMLTF MBHS_AZOCH 174 4 -
IIKVPGCPPIAEVMTGVITYMLTF O86467 188 4 -
IIKVPGCPPIAEVMTGVVTFITTF MBHS_BRAJA 189 4 -
IIKVPGCPPIAEVMTGVITYMLTF MBHS_RHOCA 188 4 -
IIKVPGCPAIAEVMTGVITYMLTF O33405 188 4 -
IIKVPGCPPIAEVMTGVVTFITTF MBHS_RHILV 188 4 -
VIKVPGCPPIAEVMTGVLTYILTF Q56359 188 4 -
IIKVPGCPPIAEVMTGVITYMLTF MBHS_RHOGE 184 4 -
IVKVPGCPPIPDVMSAIITYMVTF MBHS_CITFR 190 4 -
IIKVPGCPPIPDVMSAIITYMVTF MBHS_ECOLI 188 4 -
IIKVPGCPPIAEVMTGVIMYMVLF O66894 185 4 -
VINVPGCPPSEKNIVGNVLYYLMF O25348 212 3 -
VVNVPGCPPIGDVMVATIVHFLAL O67095 185 4 -
VINVPGCPPSEKNIVGNVLHFILF MBHS_WOLSU 180 3 -
AINIAGCPPNPMNFVGTVVHLLTK PHNS_DESGI 166 6 -
TINIPGCPPNPINFVGAVVHVLTK PHNS_DESFR 164 3 -
AINIAGCPPNPYNLVGTIVYYLKN PHNS_DESVM 194 6 -
AIVIAGCNPNTINMVGTLVAFLKG PHNS_DESDE 146 8 -

Motif 7 width=18
Element Seqn Id St Int Rpt
IPELDRQGRPKMFYSQRI MBHS_ALCEU 212 2 -
LPELDRQGRPKMFYGQRI MBHS_AZOVI 214 2 -
FPELDRQGRPKMFYSQRI MBHS_ALCHY 212 2 -
IPELDRQGRPKMFYSQRI Q51860 212 2 -
LPELDRQGRPKMFYGQRI MBHS_AZOCH 200 2 -
LPELDRQGRPAMFYSQRI O86467 214 2 -
LPELDRQGRPKMFYSQRI MBHS_BRAJA 215 2 -
MPELDRQGRPAMFYSQRI MBHS_RHOCA 214 2 -
LPELDRTGRPKMFYSQRI O33405 214 2 -
LPELDRQGPPKMFYAQPI MBHS_RHILV 214 2 -
LPELDRQGRPLMFYGQRI Q56359 214 2 -
IPELDRQGRPKMFYSQRI MBHS_RHOGE 210 2 -
LPELDRLGRPLMFYGQRI MBHS_CITFR 216 2 -
LPDVDRMGRPLMFYGQRI MBHS_ECOLI 214 2 -
IPPLDSQGRPKMFYGNRI O66894 211 2 -
LPKLDAYNRPSWAYGNRI O25348 238 2 -
LPPLDELGRPKQFYGETI O67095 211 2 -
LPSVDAFNRPMWAYGLRI MBHS_WOLSU 206 2 -
MPELDKQGRPVMFFGETV PHNS_DESGI 191 1 -
IPDLDENGRPKLFYGELV PHNS_DESFR 189 1 -
APELDSLNRPTMFFGQTV PHNS_DESVM 220 2 -
KIELDEVGRPVMFFGQSV PHNS_DESDE 171 1 -

Motif 8 width=19
Element Seqn Id St Int Rpt
HDKCYRRPHFDAGQFVEEW MBHS_ALCEU 230 0 -
HDKCYRRPHFDAGQFVEHW MBHS_AZOVI 232 0 -
HDKCYRRPHFDAGQFVESW MBHS_ALCHY 230 0 -
HDKCYRRPHFDAGQFVESW Q51860 230 0 -
HDKSYRRPHFDAGQFVEHW MBHS_AZOCH 218 0 -
HDKCYRRPHFDAGQFVEAW O86467 232 0 -
HDKCYRRPHFDAGQFVEEW MBHS_BRAJA 233 0 -
HDKCYRRPHFDAGQFVEHW MBHS_RHOCA 232 0 -
HDKCYRRPHFDAGQFVESF O33405 232 0 -
HDKCYRRPHFDAGQFVEEW MBHS_RHILV 232 0 -
HDKCYRRPHFDAGQFVESW Q56359 232 0 -
HDKCYRRPHFDAGQFVESF MBHS_RHOGE 228 0 -
HDKCYRRAHFDAGEFVESW MBHS_CITFR 234 0 -
HDKCYRRAHFDAGEFVQSW MBHS_ECOLI 232 0 -
HDTCYRRSFFNAGQFVEQF O66894 229 0 -
HDLCERRGHFDAGEFVEHF O25348 256 0 -
HDRCYRRAFYNSGQFAESF O67095 229 0 -
HDLCERRGRFDAGEFVQEF MBHS_WOLSU 224 0 -
HDNCPRLKHFEAGEFATSF PHNS_DESGI 209 0 -
HDNCPRLPHFEASEFAPSF PHNS_DESFR 207 0 -
HEQCPRLPHFDAGEFAPSF PHNS_DESVM 238 0 -
HDLCERAKHFDAGEFAPSF PHNS_DESDE 189 0 -

Motif 9 width=27
Element Seqn Id St Int Rpt
RKGFCLYKMGCKGPTTYNACSTTRWNE MBHS_ALCEU 254 5 -
RKGYCLYKVGCKGPTSYNACSTVRWNE MBHS_AZOVI 256 5 -
RKGYCLYKVGCKGPTTYNACSTTRWNG MBHS_ALCHY 254 5 -
RKGYCLYKVGCKGPTTYNACSTTRWND Q51860 254 5 -
RKGYCLYKVGCKGPTSYNACSTVRWNE MBHS_AZOCH 242 5 -
KKGYCLYKMGCKGPTTYNACSTVRWNE O86467 256 5 -
RKGYCLYKMGCKGPTTYNACSTVRWNG MBHS_BRAJA 257 5 -
RKGYCLYKMGCKGPTTYNACSTVPLER MBHS_RHOCA 256 5 -
RRGYCLYKVGCKGPTTYNACSTIRWNE O33405 256 5 -
RKGYCLYKMGCKGPTTYNACSTVRWNG MBHS_RHILV 256 5 -
RRGYCLYKVGCKGPTTYNACSTIRWNG Q56359 256 5 -
RKGFCLYKVGCKGPTTYNACSTVMWNE MBHS_RHOGE 252 5 -
RKGYCLYKMGCKGPTTYNACSTTRWNG MBHS_CITFR 258 5 -
RKGYCLYKMGCKGPTTYNACSSTRWND MBHS_ECOLI 256 5 -
KKGWCLYKVGCRGPTTYNSCGNMRWYN O66894 253 5 -
KRGFCLYKMGCKGPYTFNNCSKLRFNS O25348 280 5 -
RKGYCLYKLGCKGPITRNACASMRWNG O67095 253 5 -
KKGYCLYKVGCKGPYTFNNCSKLRFNQ MBHS_WOLSU 248 5 -
KKGYCLYELGCKGPDTYNNCPKQLFNQ PHNS_DESGI 233 5 -
KKGFCLYELGCKGPVTYNNCPKVLFNQ PHNS_DESFR 231 5 -
RKGWCLYELGCKGPVTMNNCPKIKFNQ PHNS_DESVM 262 5 -
RKGWCLYDVGCKGPETYNNCPKVLFNE PHNS_DESDE 213 5 -

Motif 10 width=14
Element Seqn Id St Int Rpt
TSFPIQSGHGCIGC MBHS_ALCEU 282 1 -
TSFPIQAGHGCIGC MBHS_AZOVI 284 1 -
TSFPIQSGHGCIGC MBHS_ALCHY 282 1 -
TSFPIQSGHGCIGC Q51860 282 1 -
TSFPIQAGHGCIGC MBHS_AZOCH 270 1 -
VSFPIQSGHGCIGC O86467 284 1 -
VSFPIQSGHGCIGC MBHS_BRAJA 285 1 -
RHFPIQSGHGCIGC MBHS_RHOCA 284 1 -
TSFPIQAGHGCIGC O33405 284 1 -
VSFPIQSGHGCIGC MBHS_RHILV 284 1 -
VSFPIQSGHGCIGC Q56359 284 1 -
TSFPIKAGHGARRS MBHS_RHOGE 280 1 -
VSFPIQSGHGCLGC MBHS_CITFR 286 1 -
VSFPIQSGHGCLGC MBHS_ECOLI 284 1 -
LSYPIQSGHGCIGC O66894 281 1 -
TSWPIGAGHGCIGC O25348 308 1 -
LSFPIQSGHPCFGC O67095 281 1 -
TSWPVQAGHGCIGC MBHS_WOLSU 276 1 -
VNWPVQAGHPCIAC PHNS_DESGI 260 0 -
VNWPVQAGHPCLGC PHNS_DESFR 258 0 -
TNWPVDAGHPCIGC PHNS_DESVM 289 0 -
TNWPVAAGHPCIGC PHNS_DESDE 240 0 -

Motif 11 width=13
Element Seqn Id St Int Rpt
SEDGFWDKGSFYD MBHS_ALCEU 296 0 -
SEDGFWDKGSFYE MBHS_AZOVI 298 0 -
SEDGFWDKGSFYS MBHS_ALCHY 296 0 -
SEDGFWDKGSFYD Q51860 296 0 -
SEDGFWDKGSFYE MBHS_AZOCH 284 0 -
SEDGFWDQGSFYD O86467 298 0 -
SEDGFWDKGSFYD MBHS_BRAJA 299 0 -
SEDGFWDQGSFYD MBHS_RHOCA 298 0 -
SEEGFWDKGSWYA O33405 298 0 -
SEDGFWDNGSFYD MBHS_RHILV 298 0 -
SEDGFWDKGSFYQ Q56359 298 0 -
SEDGFWDKGSFYD MBHS_RHOGE 293 -1 -
SENGFWDRGSFYS MBHS_CITFR 300 0 -
AENGFWDRGSFYS MBHS_ECOLI 298 0 -
AENNFWDNGPFYE O66894 295 0 -
SEPNFWDTMSPFE O25348 322 0 -
SEPDFWDRGFIYN O67095 295 0 -
SEPDFWDTMGPFE MBHS_WOLSU 290 0 -
SEPNFWDLYSPFY PHNS_DESGI 274 0 -
SEPDFWDTMTPFY PHNS_DESFR 272 0 -
SEPDFWDAMTPFY PHNS_DESVM 303 0 -
SEPNFWDDMTPFY PHNS_DESDE 254 0 -