SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00594

Identifier
P2Y2PRNOCPTR  [View Relations]  [View Alignment]  
Accession
PR00594
No. of Motifs
8
Creation Date
10-AUG-1996  (UPDATE 06-DEC-2001)
Title
P2Y2 purinoceptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01157 P2YPURNOCPTR
INTERPRO; IPR000356
GCRDB; GCR_0451; GCR_1008
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Adenosine and adenine nucleotides.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.19-31.
 
7. COMMUNI, D., JANSSENS, R., SUAREZ-HUERTA, N., ROBAYE, B. AND BOEYNAEMS, J.
Advances in signalling by extracellular nucleotides: the role and 
transduction mechanisms of P2Y receptors.
CELL SIGNAL. 12 351-360 (2000).
 
8. VON KUGELGEN, I. AND WETTER, A.
Molecular pharmacology of P2Y-receptors.
NAUNYN SCHMIEDEBERGS ARCH.PHARMACOL. 362 310-323 (2000).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
In addition to their roles in energy metabolism, extracellular nucleotides 
(such as ATP) can act as signalling molecules to induce a wide variety of 
biological effects. They are released into the extracellular fluid as a 
result of cell lysis, exocytosis of nucleotide-containing granules or by 
efflux through membrane transport proteins, and can function as autocrine 
and paracrine mediators [6,7]. Nucleotides play a role in synaptic 
transmission and in platelet-vessel wall interactions. In neurons of the 
central and peripheral nervous system, ATP is colocalised and cosecreted 
with more traditional neurotransmitters, such as catecholamines and 
acetylcholine, and has excitatory effects [6,7]. ATP is also found in high 
concentrations in the dense granules of platelets, together with ADP. These 
granules also contain lower levels of other nucleotides, such as adenine 
dinucleotides, GTP and UTP [7]. In addition, ADP is released from the 
vascular endothelium following injury and causes activation of platelets [6].
 
Receptors for adenine nucleotides are collectively termed P2 purinoceptors. 
They can be further subdivided into two structural classes: P2X receptors 
are ligand-gated ion channels, while P2Y receptors are G protein-coupled 
receptors. P2Y receptors have also been identified that are selective for 
uridine (rather than adenine) nucleotides [7]. 
 
The P2Y2 receptor is expressed widely, with highest levels in the lung,
heart, skeletal muscle, spleen, kidney, liver and epithelia. Its main role
is in regulating ion transport in airway epithelia. P2Y2 is selectively
activated by the triphosphate nucleotides UTP and ATP, and couples to Gi
and Gq proteins to activate phospholipase C [8].
 
P2Y2PRNOCPTR is an 8-element fingerprint that provides a signature for the
P2Y2 purinoceptors. The fingerprint was derived from an initial alignment of
3 sequences: the motifs were drawn from conserved sections spanning
virtually the full alignment length, focusing on those areas of the 
alignment that characterise the P2Y2 receptors but distinguish them from the
rest of the P2Y receptor family - motif 1 spans the N-terminus; motif 2
encodes the first cytoplasmic loop; motif 3 lies in the second cytoplasmic 
loop, leading into TM domain 4; motif 4 resides at the C-terminus of TM 
domain 4, leading into the second external loop; motif 5 encodes the third 
external loop; and motifs 6-8 span the C-terminus. Two iterations on 
SPTR40_18f were required to reach convergence, at which point a true set 
comprising 4 sequences was identified.
Summary Information
4 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
844444444
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
P2UR_HUMAN    P2UR_MOUSE    P2UR_RAT      Q9CPZ4        
Sequence Titles
P2UR_HUMAN  P2U purinoceptor 1 (ATP receptor) (P2U1) (Purinergic receptor) - Homo sapiens (Human). 
P2UR_MOUSE P2U purinoceptor 1 (ATP receptor) (P2U1) (Purinergic receptor) - Mus musculus (Mouse).
P2UR_RAT P2U purinoceptor 1 (ATP receptor) (P2U1) (Purinergic receptor) - Rattus norvegicus (Rat).
Q9CPZ4 PURINERGIC RECEPTOR P2Y, G-PROTEIN COUPLED 2 - Mus musculus (Mouse).
Scan History
OWL28_1    1  100  NSINGLE    
SPTR37_9f 2 140 NSINGLE
SPTR40_18f 2 100 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
WNSTINGTWEGDELGYKCR P2UR_MOUSE 8 8 -
WNSTINGTWEGDELGYKCR P2UR_RAT 8 8 -
WNDTINGTWDGDELGYRCR P2UR_HUMAN 8 8 -

Motif 2 width=12
Element Seqn Id St Int Rpt
LCRLKTWNASTT P2UR_MOUSE 59 32 -
LCRLKTWNASTT P2UR_RAT 59 32 -
LCRLKTWNASTT P2UR_HUMAN 59 32 -

Motif 3 width=10
Element Seqn Id St Int Rpt
RWGRARYARR P2UR_MOUSE 143 72 -
SWGHARYARR P2UR_RAT 143 72 -
RWGRARYARR P2UR_HUMAN 143 72 -

Motif 4 width=17
Element Seqn Id St Int Rpt
CQAPVLYFVTTSVRGTR P2UR_MOUSE 164 11 -
CQAPVLYFVTTSVRGTR P2UR_RAT 164 11 -
CQAPVLYFVTTSARGGR P2UR_HUMAN 164 11 -

Motif 5 width=11
Element Seqn Id St Int Rpt
SFRSLDLSCHT P2UR_MOUSE 270 89 -
SFRSLDLSCHT P2UR_RAT 269 88 -
SFRSLDLSCHT P2UR_HUMAN 270 89 -

Motif 6 width=14
Element Seqn Id St Int Rpt
QRLVRFARDAKPPT P2UR_MOUSE 311 30 -
QRLVRFARDAKPAT P2UR_RAT 310 30 -
QRLVRFARDAKPPT P2UR_HUMAN 311 30 -

Motif 7 width=14
Element Seqn Id St Int Rpt
QARRKLGLHRPNRT P2UR_MOUSE 331 6 -
QARRKLGLHRPNRT P2UR_RAT 330 6 -
PARRRLGLRRSDRT P2UR_HUMAN 331 6 -

Motif 8 width=13
Element Seqn Id St Int Rpt
SDDSRRTESTPAG P2UR_MOUSE 353 8 -
SDDSRRTESTPAG P2UR_RAT 354 10 -
SEDFRRTESTPAG P2UR_HUMAN 356 11 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
WNSTINGTWEGDELGYKCR P2UR_MOUSE 8 8 -
WNSTINGTWEGDELGYKCR Q9CPZ4 8 8 -
WNSTINGTWEGDELGYKCR P2UR_RAT 8 8 -
WNDTINGTWDGDELGYRCR P2UR_HUMAN 8 8 -

Motif 2 width=12
Element Seqn Id St Int Rpt
LCRLKTWNASTT P2UR_MOUSE 59 32 -
LCRLKTWNASTT Q9CPZ4 59 32 -
LCRLKTWNASTT P2UR_RAT 59 32 -
LCRLKTWNASTT P2UR_HUMAN 59 32 -

Motif 3 width=10
Element Seqn Id St Int Rpt
RWGRARYARR P2UR_MOUSE 143 72 -
RWGRARYARR Q9CPZ4 143 72 -
SWGHARYARR P2UR_RAT 143 72 -
RWGRARYARR P2UR_HUMAN 143 72 -

Motif 4 width=17
Element Seqn Id St Int Rpt
CQAPVLYFVTTSVRGTR P2UR_MOUSE 164 11 -
CQAPVLYFVTTSVRGTR Q9CPZ4 164 11 -
CQAPVLYFVTTSVRGTR P2UR_RAT 164 11 -
CQAPVLYFVTTSARGGR P2UR_HUMAN 164 11 -

Motif 5 width=11
Element Seqn Id St Int Rpt
SFRSLDLSCHT P2UR_MOUSE 270 89 -
SFRSLDLSCHT Q9CPZ4 270 89 -
SFRSLDLSCHT P2UR_RAT 269 88 -
SFRSLDLSCHT P2UR_HUMAN 270 89 -

Motif 6 width=14
Element Seqn Id St Int Rpt
QRLVRFARDAKPPT P2UR_MOUSE 311 30 -
QRLVRFARDAKPPT Q9CPZ4 311 30 -
QRLVRFARDAKPAT P2UR_RAT 310 30 -
QRLVRFARDAKPPT P2UR_HUMAN 311 30 -

Motif 7 width=14
Element Seqn Id St Int Rpt
QARRKLGLHRPNRT P2UR_MOUSE 331 6 -
QARRKLGLHRPNRT Q9CPZ4 331 6 -
QARRKLGLHRPNRT P2UR_RAT 330 6 -
PARRRLGLRRSDRT P2UR_HUMAN 331 6 -

Motif 8 width=13
Element Seqn Id St Int Rpt
SDDSRRTESTPAG P2UR_MOUSE 353 8 -
SDDSRRTESTPAG Q9CPZ4 353 8 -
SDDSRRTESTPAG P2UR_RAT 354 10 -
SEDFRRTESTPAG P2UR_HUMAN 356 11 -