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PR00511

Identifier
TEKTIN  [View Relations]  [View Alignment]  
Accession
PR00511
No. of Motifs
4
Creation Date
25-MAY-1996  (UPDATE 30-JUN-1999)
Title
Tektin signature
Database References

INTERPRO; IPR000435
Literature References
1. NORRANDER, J.M., PERRONE, C.A., AMOS, L.A. AND LINCK, R.W.
Structural comparison of tektins and evidence for their determination
of complex spacings in flagellar microtubules.
J.MOL.BIOL. 257 385-397 (1996).

Documentation
Tektin heteropolymers form unique protofilaments of flagellar microtubules
[1]. The proteins are predicted to form extended rods composed of 2 alpha-
helical segments (~180 residues long) capable of forming coiled coils,
interrupted by non-helical linkers [1]. The 2 segments are similar in 
sequence, indicating a gene duplication event. Along each tektin rod, 
cysteine residues occur with a periodicity of ~8nm, coincident with the
axial repeat of tubulin dimers in microtubules [1]. It is proposed that
the assembly of tektin heteropolymers produces filaments with repeats of
8, 16, 24, 32, 40, 48 and 96nm, generating the basis for the complex
spatial arrangements of axonemal components [1]. 
 
TEKTIN is a 4-element fingerprint that provides a signature for the
tektins. The fingerprint was derived from an initial alignment of 2
sequences: the motifs were drawn from short conserved regions in the
C-terminal half (the second segment) of the alignment - motif 1 lies in
so-called segment 2B, and motifs 2-4 span segment 2C, motif 2 including a
conserved nonapeptide. A single iteration on OWL27.1 was required to reach
convergence, no further sequences being identified beyond the starting set.
 
An update on OWL31_3 identified a true set of 2 sequences, and 1
partial match.
Summary Information
   2 codes involving  4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
42222
30000
21100
1234
True Positives
A46170        SUSMRNASEQ    
True Positive Partials
Codes involving 2 elements
S71137
Sequence Titles
A46170      tektin A1 - sea urchin (Strongylocentrotus purpuratus) 
SUSMRNASEQ SUSMRNASEQ NID: g410313 - Strongylocentrotus purpuratus cDNA to...

S71137 tectin C1 - sea urchin (Strongylocentrotus purpuratus)
Scan History
OWL27_1    1  50   NSINGLE    
OWL31_3 2 50 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
QAVKDKEAPMKVAQTRL A46170 356 356 -
QAIKDKENPLKLAMTRL SUSMRNASEQ 275 275 -

Motif 2 width=21
Element Seqn Id St Int Rpt
RTHRPNVELCRDPAQYRMVQE A46170 375 2 -
RTYRPNVELCRDNAQYGLVNE SUSMRNASEQ 294 2 -

Motif 3 width=17
Element Seqn Id St Int Rpt
EIQDSIDKLQQKLAESK A46170 398 2 -
EIQDSIKALEKKLQDAH SUSMRNASEQ 317 2 -

Motif 4 width=13
Element Seqn Id St Int Rpt
KEIALKKNSIFVD A46170 429 14 -
KDLELKNNSLDLD SUSMRNASEQ 348 14 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
QAVKDKEAPMKVAQTRL A46170 356 356 -
QAIKDKENPLKLAMTRL SUSMRNASEQ 275 275 -

Motif 2 width=21
Element Seqn Id St Int Rpt
RTHRPNVELCRDPAQYRMVQE A46170 375 2 -
RTYRPNVELCRDNAQYGLVNE SUSMRNASEQ 294 2 -

Motif 3 width=17
Element Seqn Id St Int Rpt
EIQDSIDKLQQKLAESK A46170 398 2 -
EIQDSIKALEKKLQDAH SUSMRNASEQ 317 2 -

Motif 4 width=13
Element Seqn Id St Int Rpt
KEIALKKNSIFVD A46170 429 14 -
KDLELKNNSLDLD SUSMRNASEQ 348 14 -