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PR00497

Identifier
P40PHOX  [View Relations]  [View Alignment]  
Accession
PR00497
No. of Motifs
6
Creation Date
10-MAR-1996  (UPDATE 07-JUN-1999)
Title
Neutrophil cytosol factor P40 signature
Database References

INTERPRO; IPR000919
Literature References
1. SEGAL, A.W.
The NADPH oxidase and chronic granulomatous disease.
MOL.MED.TODAY 2(3) 129-135 (1996).

Documentation
Phagocytes form the first line of defence against invasion by micro-
organisms. Engulfing of bacteria by neutrophils is accompanied by the
consumption of large amounts of oxygen - a so-called respiratory burst.
Defects in phagocytosis involving the lack of a respiratory burst give
rise to chronic granulomatous disease (CGD), in which patients are pre-
disposed to infection, often from otherwise non-pathogenic bacteria [1].
 
Regulation of the respiratory burst takes place at the phagocytic vacuole.
The process is mediated by NADPH oxidase, which transports electrons across
the plasma membrane to form superoxide (an oxygen molecule with an extra
electron that is toxic to normal cells) in the vacuole interior. The
electrons are carried across the membrane by a short electron transport
chain in the form of an unusual flavocytochrome b [1].
 
To conserve NADPH and avoid the toxic effects of superoxide, the oxidase 
remains inactive until it receives an appropriate stimulus. Activation
involves the participation of a number of cytosolic proteins, some of 
which attach to the flavocytochrome. P47phox, p67phox and p40phox are
specialised components of phagocytic cells. All contain SH3 domains, by
means of which they attach to proline-rich regions of other proteins. Upon
activation, p40phox translocates to the region of the plasma membrane
forming the phagocytic vacuole, where, with phosphorylated p47phox and
p67phox, it associates with hydrophilic regions of the flavocytochrome.
 
P40PHOX is a 6-element fingerprint that provides a signature for neutrophil
cytosol factor p40 (p40phox). The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from short conserved regions
spanning the full alignment length. A single iteration on OWL27.0 was
required to reach convergence, no further sequences being identified beyond
the starting set.
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6333333
5000000
4000000
3000000
2000000
123456
True Positives
O60808        P97369        Q15080        
Sequence Titles
O60808      BK833B7.1 - HOMO SAPIENS (HUMAN).             
P97369 NEUTROPHIL CYTOSOLIC FACTOR 4 (P40-PHOX) - MUS MUSCULUS (MOUSE).
Q15080 P40PHOX PROTEIN - HOMO SAPIENS (HUMAN).
Scan History
OWL27_0    1  25   NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
YRRYRQFHALQSKLEERF S39768 56 56 -
YRRYRQFHALQSKLEERF S39769 5 5 -

Motif 2 width=19
Element Seqn Id St Int Rpt
GPDSKSSALACTLPTLPAK S39768 74 0 -
GPDSKSSALACTLPTLPAK S39769 23 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
IFFYQSPYDSEQVPQA S39768 131 38 -
IFFYQSPYDSEQVPQA S39769 53 11 -

Motif 4 width=22
Element Seqn Id St Int Rpt
KAGDVIFLLSRINKDWLEGTVR S39768 192 45 -
KAGDVIFLLSRINKDWLEGTVR S39769 80 11 -

Motif 5 width=20
Element Seqn Id St Int Rpt
RCYYYEDTISTIKDIAVEED S39768 241 27 -
RCYYYEDTISTIKDIAVEED S39769 109 7 -

Motif 6 width=12
Element Seqn Id St Int Rpt
LLSDEDVALMVR S39768 297 36 -
LLSDEDVALMVR S39769 132 3 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
YRRYRQFHALQSKLEERF O60808 56 56 -
YRRYRQFHALQSKLEERF Q15080 56 56 -
YRRYRQFYALQSKLEERF P97369 56 56 -

Motif 2 width=19
Element Seqn Id St Int Rpt
GPDSKSSALACTLPTLPAK O60808 74 0 -
GPDSKSSALACTLPTLPAK Q15080 74 0 -
GPESKNSPFTCNLPTLPAK P97369 74 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
IFFYQSPYDSEQVPQA O60808 131 38 -
IFFYQSPYDSEQVPQA Q15080 131 38 -
IFFYQSAYDAEQVPQA P97369 131 38 -

Motif 4 width=22
Element Seqn Id St Int Rpt
KAGDVIFLLSRINKDWLEGTVR O60808 192 45 -
KAGDVIFLLSRINKDWLEGTVR Q15080 192 45 -
KAGDVIFLLSKINKDWLEGTSQ P97369 192 45 -

Motif 5 width=20
Element Seqn Id St Int Rpt
RCYYYEDTISTIKDIAVEED O60808 241 27 -
RCYYYEDTISTIKDIAVEED Q15080 241 27 -
RCYFYEDTGKTIKDIAVEED P97369 241 27 -

Motif 6 width=12
Element Seqn Id St Int Rpt
LLSDEDVALMVR O60808 297 36 -
LLSDEDVALMVR Q15080 297 36 -
LLSDEDVGLMVK P97369 297 36 -