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PR00492

Identifier
RHOGDI  [View Relations]  [View Alignment]  
Accession
PR00492
No. of Motifs
6
Creation Date
22-MAR-1996  (UPDATE 10-JUN-1999)
Title
RHO protein GDP dissociation inhibitor signature
Database References

INTERPRO; IPR000406
Literature References
1. FUKUMOTO, Y., KAIBUCHI, K., HORI, Y., FUJIOKA, H., ARAKI, S., UEDA, T.,
KIKUCHI, A. AND TAKAI, Y.
Molecular cloning and characterization of a novel type of regulatory
protein (GDI) for the rho proteins, ras p21-like small GTP-binding proteins.
ONCOGENE 5 1321-1328 (1990).
 
2. PICK, E., GORZALCZANY, Y. AND ENGEL, S.
Role of the rac1 p21-GDP-dissociation inhibitor for rho heterodimer in the
activation of the superoxide-forming NADPH oxidase of macrophages.
EUR.J.BIOCHEMISTRY 217 441-455 (1993).
 
3. SEGAL, A.W.
The NADPH oxidase and chronic granulomatous disease.
MOL.MED.TODAY 2(3) 129-135 (1996).

Documentation
The GDP dissociation inhibitor for rho proteins, rho GDI, regulates GDP/GTP
exchange. The protein contains 204 amino acids, with a calculated Mr value
of 23,421. Hydropathy analysis shows it to be largely hydrophilic, with a
single hydrophobic region. Results of database searches suggest rho GDI is
a novel protein, currently with no known homologue. 
 
The protein plays an important role in the activation of the superoxide
(O2-)-generating NADPH oxidase of phagocytes. This process requires the
interaction of membrane-associated cytochrome b559 with 3 cytosolic
components: p47-phox, p67-phox and a heterodimer of the small G protein
p21rac1 and rho GDI [2]. The association of p21rac and GDI inhibits
dissociation of GDP from p21rac, thereby maintaining it in an inactive form.
The proteins are attached via a lipid tail on p21rac that binds to the
hydrophobic region of GDI [3]. Dissociation of these proteins might be
mediated by the release of lipids (e.g., arachidonate and phosphatidate)
from membranes through the action of phospholipases [3]. The lipids may then
compete with the lipid tail on p21rac for the hydrophobic pocket on GDI. 
 
RHOGDI is a 6-element fingerprint that provides a signature for rho protein
GDP dissociation inhibitors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from short conserved
regions spanning the full alignment length. Two iterations on OWL27.0 were
required to reach convergence, at which point a true set comprising 8
sequences was identified. Three partial matches were also found, all of
which are rhoGDI fragments. 
 
An update on SPTR37_9f identified a true set of 6 sequences, and 3
partial matches.
Summary Information
   6 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
6666666
5000000
4000000
3010101
2001201
123456
True Positives
GDIR_BOVIN    GDIR_HUMAN    GDIS_HUMAN    GDIS_MOUSE    
GDIT_HUMAN GDIT_MOUSE
True Positive Partials
Codes involving 3 elements
GDIR_YEAST
Codes involving 2 elements
GDIR_CAEEL O14224
Sequence Titles
GDIR_BOVIN  RHO GDP-DISSOCIATION INHIBITOR 1 (RHO GDI 1) (RHO-GDI ALPHA) - BOS TAURUS (BOVINE). 
GDIR_HUMAN RHO GDP-DISSOCIATION INHIBITOR 1 (RHO GDI 1) (RHO-GDI ALPHA) - HOMO SAPIENS (HUMAN).
GDIS_HUMAN RHO GDP-DISSOCIATION INHIBITOR 2 (RHO GDI 2) (RHO-GDI BETA) (LY-GDI) - HOMO SAPIENS (HUMAN).
GDIS_MOUSE RHO GDP-DISSOCIATION INHIBITOR 2 (RHO GDI 2) (RHO-GDI BETA) (D4) - MUS MUSCULUS (MOUSE).
GDIT_HUMAN RHO GDP-DISSOCIATION INHIBITOR 3 (RHO GDI 3) (RHO-GDI GAMMA) - HOMO SAPIENS (HUMAN).
GDIT_MOUSE RHO GDP-DISSOCIATION INHIBITOR 3 (RHO GDI 3) (RHO-GDI GAMMA) (RHO- GDI2) - MUS MUSCULUS (MOUSE).

GDIR_YEAST RHO GDP-DISSOCIATION INHIBITOR (RHO GDI) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).

GDIR_CAEEL PROBABLE RHO GDP-DISSOCIATION INHIBITOR (RHO GDI) - CAENORHABDITIS ELEGANS.
O14224 PUTATIVE RHO GDP-DISSOCIATION INHIBITOR (RHO GDI) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
Scan History
OWL27_1    2  100  NSINGLE    
SPTR37_9f 2 66 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
AVSADPNVPNVVVTRL GDIR_BOVIN 60 60 -
AVSADPNEPNVIVTRL GDIR_CAVPO 2 2 -
AVSADPNEPNVIVTRL S38768 2 2 -

Motif 2 width=19
Element Seqn Id St Int Rpt
TLVCSTAPGPLELDLTGDL S38768 18 0 -
TLVCSTAPGPLELDLTGDL GDIR_BOVIN 76 0 -
TLVCSTAPGPLELDLTGDL GDIR_CAVPO 18 0 -

Motif 3 width=17
Element Seqn Id St Int Rpt
IVSGMKYIQHTYRKGVK GDIR_BOVIN 122 27 -
IKISFRYIQHTYRKGVK GDIR_CAVPO 41 4 -
IKISFRYIQHTYRKGVK S38768 41 4 -

Motif 4 width=16
Element Seqn Id St Int Rpt
IDKTDYMVGSYGPRAE S38768 58 0 -
IDKTDYMVGSYGPRAE GDIR_BOVIN 139 0 -
IDKTDYMVGSYGPRAE GDIR_CAVPO 58 0 -

Motif 5 width=13
Element Seqn Id St Int Rpt
EYEFLTPMEEAPK S38768 74 0 -
EYEFLTPMEEAPK GDIR_CAVPO 74 0 -
EYEFLTPMEEAPK GDIR_BOVIN 155 0 -

Motif 6 width=18
Element Seqn Id St Int Rpt
FTDDDKTDHLSGERNLTI GDIR_CAVPO 92 5 -
FTDDDRTDHLSWEWNLTI GDIR_BOVIN 181 13 -
FTDDDKTDHLSGERNLTI S38768 87 0 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
PVVTDPKAPNVVVTRL GDIS_HUMAN 57 57 -
AVSADPNVPNVVVTGL GDIR_HUMAN 60 60 -
AVSADPNVPNVVVTRL GDIR_BOVIN 60 60 -
PVVADPTVPNVTVTRL GDIS_MOUSE 56 56 -
PPAVDPSLPNVQVTRL GDIT_HUMAN 81 81 -
PPIMDPSLPNVQVTRL GDIT_MOUSE 81 81 -

Motif 2 width=19
Element Seqn Id St Int Rpt
TLVCESAPGPITMDLTGDL GDIS_HUMAN 73 0 -
TLVCSSAPGPLELDLTGDL GDIR_HUMAN 76 0 -
TLVCSTAPGPLELDLTGDL GDIR_BOVIN 76 0 -
SLVCDSAPGPITMDLTGDL GDIS_MOUSE 72 0 -
TLLSEQAPGPVVMDLTGNL GDIT_HUMAN 97 0 -
TLLTEQAPGPIIMDLTGDL GDIT_MOUSE 97 0 -

Motif 3 width=17
Element Seqn Id St Int Rpt
IVSGLKYVQHTYRTGVK GDIS_HUMAN 119 27 -
IVSGMKYIQHTYRKGVK GDIR_HUMAN 122 27 -
IVSGMKYIQHTYRKGVK GDIR_BOVIN 122 27 -
IVSGLKYVQHTYRTGMR GDIS_MOUSE 118 27 -
IVSGLKCLHHTYRRGLR GDIT_HUMAN 143 27 -
IVSGLKCLHHTYRRGLR GDIT_MOUSE 143 27 -

Motif 4 width=16
Element Seqn Id St Int Rpt
VDKATFMVGSYGPRPE GDIS_HUMAN 136 0 -
IDKTDYMVGSYGPRAE GDIR_HUMAN 139 0 -
IDKTDYMVGSYGPRAE GDIR_BOVIN 139 0 -
VDKATFMVGSYGPRPE GDIS_MOUSE 135 0 -
VDKTVYMVGSYGPSAQ GDIT_HUMAN 160 0 -
VDKAIFMVGSYGPRAQ GDIT_MOUSE 160 0 -

Motif 5 width=13
Element Seqn Id St Int Rpt
EYEFLTPVEEAPK GDIS_HUMAN 152 0 -
EYEFLTPVEEAPK GDIR_HUMAN 155 0 -
EYEFLTPMEEAPK GDIR_BOVIN 155 0 -
EYEFLTPVEEAPK GDIS_MOUSE 151 0 -
EYEFVTPVEEAPR GDIT_HUMAN 176 0 -
EYEFVTSVEEAPR GDIT_MOUSE 176 0 -

Motif 6 width=18
Element Seqn Id St Int Rpt
FTDDDKQDHLSWEWNLSI GDIS_HUMAN 178 13 -
FTDDDKTDHLSWEWNLTI GDIR_HUMAN 181 13 -
FTDDDRTDHLSWEWNLTI GDIR_BOVIN 181 13 -
FTDDDKQDHLTWEWNLAI GDIS_MOUSE 177 13 -
FTDDDRTHHLSWEWGLCI GDIT_HUMAN 202 13 -
FTDDDRLNHLSWEWHLHV GDIT_MOUSE 202 13 -