| Identifier | LUPUSLA [View Relations] [View Alignment]
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| Accession | PR00302 |
| No. of Motifs | 5 |
| Creation Date | 02-FEB-1995 (UPDATE 07-JUN-1999) |
| Title | Lupus La protein signature |
| Database References |
PROSITE; PS00030 RNP_1
BLOCKS; BL00030
INTERPRO; IPR002344
|
| Literature References | 1. PRUIJN, G.J.M., SLOBBE, R.L. AND VAN VENROOIJ, W.J.
Structure and function of La and Ro RNPs.
MOL.BIOL.REP. 14 43-48 (1990).
2. LIN-MARQ, N. AND CLARKSON, S.G.
A yeast RNA binding protein that resembles the human autoantigen La.
J.MOL.BIOL. 244(5) 81-85 (1995).
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| Documentation | The La protein is a 47kDa polypeptide that often acts as an autoantigen
in systemic lupus erythematosus and Sjogren's syndrome patients [1]. A
vital property of the protein is its association with the U-rich termini
of newly-synthesised RNA polymerase III transcripts. A 32kDa yeast
protein shows similarity to the N-termini of vertebrate La proteins,
and also preferentially binds U-rich RNAs [2].
LUPUSLA is a 5-element fingerprint that provides a signature for the
lupus La proteins and the related La protein homologue. The fingerprint
was derived from an initial alignment of 4 sequences: the motifs were
drawn from conserved regions in the N-terminal region of the alignment,
motifs 4 and 5 each including an RNA-binding region (cf. PROSITE pattern
RNP_1 (PS00030)). Two iterations on OWL25.1 were required to reach
convergence, at which point a true set comprising 9 sequences was
identified.
An update on SPTR37_9f identified a true set of 11 sequences.
|
| Summary Information | 11 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
|
| Composite Feature Index | | 5 | 11 | 11 | 11 | 11 | 11 | | 4 | 0 | 0 | 0 | 0 | 0 | | 3 | 0 | 0 | 0 | 0 | 0 | | 2 | 0 | 0 | 0 | 0 | 0 | | 1 | 2 | 3 | 4 | 5 |
|
| True Positives | LAA_XENLA LAB_XENLA LAH1_SCHPO LAH1_YEAST
LA_AEDAL LA_BOVIN LA_DROME LA_HUMAN
LA_MOUSE LA_RAT O01806 |
| Sequence Titles | LAA_XENLA LUPUS LA PROTEIN HOMOLOG A (LA RIBONUCLEOPROTEIN A) (LA AUTOANTIGEN HOMOLOG A) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
LAB_XENLA LUPUS LA PROTEIN HOMOLOG B (LA RIBONUCLEOPROTEIN B) (LA AUTOANTIGEN HOMOLOG B) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
LAH1_SCHPO LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN HOMOLOG) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
LAH1_YEAST LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN HOMOLOG) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
LA_AEDAL LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN HOMOLOG) - AEDES ALBOPICTUS (FOREST DAY MOSQUITO).
LA_BOVIN LUPUS LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN HOMOLOG) - BOS TAURUS (BOVINE).
LA_DROME LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN HOMOLOG) - DROSOPHILA MELANOGASTER (FRUIT FLY).
LA_HUMAN LUPUS LA PROTEIN (SJOGREN SYNDROME TYPE B ANTIGEN (SS-B)) (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN) - HOMO SAPIENS (HUMAN).
LA_MOUSE LUPUS LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN HOMOLOG) - MUS MUSCULUS (MOUSE).
LA_RAT LUPUS LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN HOMOLOG) - RATTUS NORVEGICUS (RAT).
O01806 SIMILARITY TO AN RNA RECOGNITION MOTIF - CAENORHABDITIS ELEGANS.
|
| Scan History | OWL25_1 2 50 NSINGLE
SPTR37_9f 2 12 NSINGLE
|
| Initial Motifs | Motif 1 width=18
Element Seqn Id St Int Rpt
QIEYYFGDFNLPRDKFLK LA_HUMAN 20 20 -
QIEYYFGDFNLPRDKFLK LA_BOVIN 20 20 -
QIEYYFGDHNLPRDKFLK LAB_XENLA 19 19 -
QIEYYFGDHNLPRDKFLK LAA_XENLA 20 20 -
Motif 2 width=16
Element Seqn Id St Int Rpt
GWVPLEIMIKFNRLNR LA_HUMAN 45 7 -
GWVPLEIMIKFNRLNR LA_BOVIN 45 7 -
GWVPLETMIKFNRLSK LAB_XENLA 44 7 -
GWVPLETMIKFNRLSK LAA_XENLA 45 7 -
Motif 3 width=14
Element Seqn Id St Int Rpt
ELMEISEDKTKIRR LA_HUMAN 78 17 -
ELMEISEDKTKIRR LA_BOVIN 78 17 -
ELLEINEEKCKIRR LAB_XENLA 77 17 -
ELLEINEEKCKIRR LAA_XENLA 78 17 -
Motif 4 width=17
Element Seqn Id St Int Rpt
KNDVKNRSVYIKGFPTD LA_HUMAN 105 13 -
KNDVKNRSVYIKGFPTD LA_BOVIN 105 13 -
KNSFKHRSVYIKGFPTI LAB_XENLA 104 13 -
KNSLKHKSVYIKGFPTS LAA_XENLA 105 13 -
Motif 5 width=19
Element Seqn Id St Int Rpt
FKGSIFVVFDSIESAKKFV LA_HUMAN 150 28 -
FKGSIFAVFDSIESAKKFV LA_BOVIN 150 28 -
FKGSVFLVFNTEDGAKKFL LAB_XENLA 149 28 -
FKGSIFIIFNTDDDAKKFL LAA_XENLA 150 28 -
|
| Final Motifs | Motif 1 width=18
Element Seqn Id St Int Rpt
QIEYYFGDFNLPRDKFLK LA_HUMAN 20 20 -
QIEYYFGDFNLPRDKFLK LA_BOVIN 20 20 -
QIEYYFGDFNLPRDKFLK LA_MOUSE 20 20 -
QIEYYFGDFNLPRDKFLK LA_RAT 20 20 -
QIEYYFGDHNLPRDKFLK LAB_XENLA 19 19 -
QIEYYFGDHNLPRDKFLK LAA_XENLA 20 20 -
QVEYYFGDANLNRDKFLR LA_DROME 57 57 -
QLEYYFGDANLARDKFLQ LA_AEDAL 50 50 -
QLEYYFGNINLPRDKFLQ O01806 19 19 -
QVEFYFSDTNLPHDKFLW LAH1_SCHPO 70 70 -
QVEFYFSEFNFPYDRFLR LAH1_YEAST 36 36 -
Motif 2 width=16
Element Seqn Id St Int Rpt
GWVPLEIMIKFNRLNR LA_HUMAN 45 7 -
GWVPLEIMIKFNRLNR LA_BOVIN 45 7 -
GWVPLETMIKFNRLNR LA_MOUSE 45 7 -
GWVPLETMIKFNRLNR LA_RAT 45 7 -
GWVPLETMIKFNRLSK LAB_XENLA 44 7 -
GWVPLETMIKFNRLSK LAA_XENLA 45 7 -
GWVPLSVLVTFKRLAS LA_DROME 83 8 -
GWVPVDVLLTFKRLKS LA_AEDAL 75 7 -
GWVPITTMLNFNRLAS O01806 44 7 -
GWVPIQTIANFKRMRR LAH1_SCHPO 95 7 -
GWVPISTIATFNRMKK LAH1_YEAST 61 7 -
Motif 3 width=14
Element Seqn Id St Int Rpt
ELMEISEDKTKIRR LA_HUMAN 78 17 -
ELMEISEDKTKIRR LA_BOVIN 78 17 -
KLMEVSADKTKIRR LA_MOUSE 78 17 -
NLMEVSADKTKIRR LA_RAT 78 17 -
ELLEINEEKCKIRR LAB_XENLA 77 17 -
ELLEINEEKCKIRR LAA_XENLA 78 17 -
GLVEISEDKLSLRR LA_DROME 116 17 -
GLIEVSEDREKLRR LA_AEDAL 108 17 -
GIISVSEDNQKIRR O01806 77 17 -
ELLEVDEAGEKVRR LAH1_SCHPO 126 15 -
EILEVSADGENVKR LAH1_YEAST 91 14 -
Motif 4 width=17
Element Seqn Id St Int Rpt
KNDVKNRSVYIKGFPTD LA_HUMAN 105 13 -
KNDVKNRSVYIKGFPTD LA_BOVIN 105 13 -
KNDVKNRSVYIKGFPTD LA_MOUSE 105 13 -
KNDVKNRSVYIKGFPTD LA_RAT 105 13 -
KNSFKHRSVYIKGFPTI LAB_XENLA 104 13 -
KNSLKHKSVYIKGFPTS LAA_XENLA 105 13 -
RKEIQERTAYAKGFPLD LA_DROME 143 13 -
RKEIYGRTVYVKGFAPE LA_AEDAL 135 13 -
WQKIKHRTVYMKGFSTD O01806 104 13 -
NKSVMERSVYCKGFGDE LAH1_SCHPO 148 8 -
RIEQNQRTLAVMNFPHE LAH1_YEAST 117 12 -
Motif 5 width=19
Element Seqn Id St Int Rpt
FKGSIFVVFDSIESAKKFV LA_HUMAN 150 28 -
FKGSIFAVFDSIESAKKFV LA_BOVIN 150 28 -
FKGSIFAVFDSIQSAKKFV LA_MOUSE 150 28 -
FKGSIFAVFDSIQSAKKFV LA_RAT 150 28 -
FKGSVFLVFNTEDGAKKFL LAB_XENLA 149 28 -
FKGSIFIIFNTDDDAKKFL LAA_XENLA 150 28 -
FKGSIFLTFETKDQAKAFL LA_DROME 193 33 -
FKGSVFVTFATKDHCAEFL LA_AEDAL 187 35 -
FKGSVFITYKTREEAEAAQ O01806 151 30 -
FKGSVFVEFKEPDVANKFL LAH1_SCHPO 196 31 -
FNGTVLVEFKTIPECEAFL LAH1_YEAST 171 37 -
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