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PR00265

Identifier
INTERLEUKIN2  [View Relations]  [View Alignment]  
Accession
PR00265
No. of Motifs
5
Creation Date
01-FEB-1994  (UPDATE 07-JUN-1999)
Title
Interleukin-2 signature
Database References

PROSITE; PS00424 INTERLEUKIN_2
BLOCKS; BL00424
PFAM; PF00715 IL2
INTERPRO; IPR000779
PDB; 3INK
SCOP; 3INK
CATH; 3INK
Literature References
1. YOKOTA, T., ARAI, N., LEE, F., RENNICK, D., MOSMANN, T. AND ARAI, K.
Use of a cDNA expression vector for isolation of mouse interleukin-2 cDNA
clones - expression of T-cell growth factor activity after transfection of
monkey cells.
PROC.NATL.ACAD.SCI.U.S.A. 82(1) 68-72 (1985).
 
2. CERRETTI, D.P., MCKEREGHAN, K., LARSEN, A., CANTRELL, M.A., ANDERSON, D.,
GILLIS, S., COSMAN, D. AND BAKER, P.E.
Cloning, sequence, and expression of bovine interleukin 2
PROC.NATL.ACAD.SCI.U.S.A. 83(10) 3223-3227 (1986).
 
3. MOTT, H.R., DRISCOLL, P.C., BOYD, J., COOKE, R.M., WEIR, M.P. AND
CAMPBELL, I.D.
Secondary structure of human interleukin 2 from 3D heteronuclear NMR
experiments.
BIOCHEMISTRY 31(33) 7741-7744 (1992).

Documentation
T-Lymphocytes regulate the growth and differentiation of certain lympho-
poietic and haemopoietic cells through the release of various secreted
protein factors [1]. These factors, which include interleukin-2 (IL2), are
secreted by lectin- or antigen-stimulated T-cells, and have various 
physiological effects. IL2 is a lymphokine that induces the proliferation
of responsive T-cells. In addition, it acts on some B-cells, via receptor-
specific binding [2], as a growth factor and antibody production stimulant
[3]. The protein is secreted as a single glycosylated polypeptide, cleavage
of a signal sequence being required for its activity, which is partially
species restricted [2]. 
 
Solution NMR suggests that IL2 is a mainly alpha-helical protein, its
structure comprising a bundle of 4 helices (termed A-D), flanked by 2
shorter helices and several poorly-defined loops. Residues in helix A, and
in the loop region between helices A and B, are important for receptor
binding [3]. Secondary structure analysis has suggested similarity to IL4
and granulocyte-macrophage colony stimulating factor (GMCSF) [3]. 
 
INTERLEUKIN2 is a 5-element fingerprint that provides a signature for the
IL2 cytokine family. The fingerprint was derived from an initial alignment
of 4 sequences: motif 1 spans the signal peptide and the highly-conserved
N-terminal PTSSST sequence; motif 3 includes the region encoded by PROSITE
pattern INTERLEUKIN_2 (PS00424); and motifs 3 and 4 each contain a Cys
residue required to form a disulphide bridge that stabilises the active
form of the peptide. Two iterations on OWL22.1 were required to reach
convergence, at which point a true set comprising 11 sequences was
identified. Five partial matches were also found: S34052, SYNDABILA,
SYNDABILB and SYNDABILC are IL2 sequences and fusion proteins that lack
the signal peptide; and S31209 is an IL2 fusion protein fragment.
 
An update on SPTR37_9f identified a true set of 19 sequences.
Summary Information
19 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
51919191919
400000
300000
200000
12345
True Positives
IL2_BOVIN     IL2_CANFA     IL2_CAPHI     IL2_CEREL     
IL2_CERTO IL2_FELCA IL2_HORSE IL2_HUMAN
IL2_MACFA IL2_MACMU IL2_MERUN IL2_MIRAN
IL2_MOUSE IL2_PIG IL2_RAT IL2_SHEEP
O77620 O88210 Q13169
Sequence Titles
IL2_BOVIN   INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - BOS TAURUS (BOVINE). 
IL2_CANFA INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - CANIS FAMILIARIS (DOG).
IL2_CAPHI INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - CAPRA HIRCUS (GOAT).
IL2_CEREL INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - CERVUS ELAPHUS (RED DEER).
IL2_CERTO INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - CERCOCEBUS TORQUATUS ATYS (RED-CROWNED MANGABEY) (SOOTY MANGABEY).
IL2_FELCA INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - FELIS SILVESTRIS CATUS (CAT).
IL2_HORSE INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - EQUUS CABALLUS (HORSE).
IL2_HUMAN INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) (ALDESLEUKIN) - HOMO SAPIENS (HUMAN), AND HYLOBATES LAR (COMMON GIBBON).
IL2_MACFA INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - MACACA FASCICULARIS (CRAB EATING MACAQUE) (CYNOMOLGUS MONKEY).
IL2_MACMU INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - MACACA MULATTA (RHESUS MACAQUE), AND MACACA NEMESTRINA (PIG-TAILED MACAQUE).
IL2_MERUN INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - MERIONES UNGUICULATUS (MONGOLIAN JIRD).
IL2_MIRAN INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - MIROUNGA ANGUSTIROSTRIS (NORTHERN ELEPHANT SEAL).
IL2_MOUSE INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - MUS MUSCULUS (MOUSE).
IL2_PIG INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - SUS SCROFA (PIG).
IL2_RAT INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - RATTUS NORVEGICUS (RAT).
IL2_SHEEP INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - OVIS ARIES (SHEEP).
O77620 INTERLEUKIN-2 PRECURSOR (IL-2) (T-CELL GROWTH FACTOR) (TCGF) - ORYCTOLAGUS CUNICULUS (RABBIT).
O88210 INTERLEUKIN 2 PRECURSOR - CAVIA PORCELLUS (GUINEA PIG).
Q13169 INTERLEUKIN 2 - HOMO SAPIENS (HUMAN).
Scan History
OWL22_1    2  100  NSINGLE    
OWL26_0 1 100 NSINGLE
SPTR37_9f 2 20 NSINGLE
Initial Motifs
Motif 1  width=29
Element Seqn Id St Int Rpt
YRMQLLSCIALSLALVTNSAPTSSSTKKT IL2_HUMAN 2 2 -
YKMQLLCCIALTLALMANGAPTSSSTKNT IL2_PIG 2 2 -
YKIQLLSCIALTLALVANGAPTSSSTGNT IL2_BOVIN 2 2 -
YKIQPLSCIALTLALVANGAPTSSSTGNT IL2_SHEEP 2 2 -

Motif 2 width=16
Element Seqn Id St Int Rpt
NPKLTRMLTFKFYMPK IL2_HUMAN 53 22 -
NADLSRMLTFKFYMPK IL2_PIG 53 22 -
NLKLSRMHTFDFYVPK IL2_BOVIN 53 22 -
NLKLSRMHTFNFYMPK IL2_SHEEP 53 22 -

Motif 3 width=28
Element Seqn Id St Int Rpt
ATELKHLQCLEEELKPLEEVLNLAQSKN IL2_HUMAN 70 1 -
ATELKHLQCLVEELKALEGVLNLGQSKN IL2_PIG 70 1 -
ATELKHLKCLLEELKLLEEVLNLAPSKN IL2_BOVIN 71 2 -
ATELKHLKCLLEELKLLEEVLDLAPSKN IL2_SHEEP 71 2 -

Motif 4 width=22
Element Seqn Id St Int Rpt
NINVIVLELKGSETTFMCEYAD IL2_HUMAN 108 10 -
NINVTVLELKGSETSFKCEYDD IL2_PIG 109 11 -
NIKRIVLELQGSETRFTCEYDD IL2_BOVIN 110 11 -
NIKRIVLELQGSETRFTCEYDD IL2_SHEEP 110 11 -

Motif 5 width=22
Element Seqn Id St Int Rpt
TATIVEFLNRWITFCQSIISTL IL2_HUMAN 131 1 -
TVTAVEFLNKWITFCQSIYSTL IL2_PIG 132 1 -
TVNAVEFLNKWITFCQSIYSTM IL2_BOVIN 133 1 -
TVKAVEFLNKWITFCQSIYSTM IL2_SHEEP 133 1 -
Final Motifs
Motif 1  width=29
Element Seqn Id St Int Rpt
YRMQLLSCIALSLALVTNSAPTSSSTKKT IL2_HUMAN 2 2 -
YRMQLLSCIALSLALVTNSAPTSSSTKKT IL2_MACMU 2 2 -
YRMQLLSCIALILALVTNSAPTSSSTKKT Q13169 2 2 -
YRMQLLSCIALSLALVTNSAPTSRSTKKT IL2_CERTO 2 2 -
YRMQLLSCIALSLALVTNSAPTSSSTKKT IL2_MACFA 2 2 -
YKIQLLSCIALTLILVTNSAPASSSTKET IL2_FELCA 2 2 -
YKMQLLCCIALTLALMANGAPTSSSTKNT IL2_PIG 2 2 -
CKMQLLSCIALSLVLVANSAPTTSSTKET IL2_MIRAN 2 2 -
YKIQLLSCIALTLALVANGAPTSSSTGNT IL2_BOVIN 2 2 -
YKIQPLSCIALTLALVANGAPTSSSTGNT IL2_SHEEP 2 2 -
YKVQLLSCIALTLALLTSSAPTSSSTKET O77620 2 2 -
YRMQLLSCIALTLAVLANSAPTSSSKRET IL2_HORSE 2 2 -
YKMQLLSCIALTLVLVANSAPITSSSTKE IL2_CANFA 2 2 -
YRMQLLSCIALTLALVANGAPTSSSTGNT IL2_CAPHI 2 2 -
YKIQLLSCIALTLALVANGAPTSSSTGNT IL2_CEREL 2 2 -
YSRQLASCVALALVLLANSAPTSSPAKEA IL2_MERUN 2 2 -
YSMQLASCVALTLVLLVNSAPTSSPAKET IL2_RAT 2 2 -
YSMQLASCVTLTLVLLVNSAPTSSSTSSS IL2_MOUSE 2 2 -
YKTLLLSCLALTLALLTSSAPTSSSPKQT O88210 2 2 -

Motif 2 width=16
Element Seqn Id St Int Rpt
NPKLTRMLTFKFYMPK IL2_HUMAN 53 22 -
NPKLTRMLTFKFYMPK IL2_MACMU 53 22 -
NPKLTRMLTFKFYMPK Q13169 56 25 -
NPKLTRMLTFKFYMPK IL2_CERTO 53 22 -
NPKLTRMLTFKFYMPK IL2_MACFA 53 22 -
NPKLSRMLTFKFYVPK IL2_FELCA 53 22 -
NADLSRMLTFKFYMPK IL2_PIG 53 22 -
DPKLSRMLTFKFYTPK IL2_MIRAN 53 22 -
NLKLSRMHTFDFYVPK IL2_BOVIN 53 22 -
NLKLSRMHTFNFYMPK IL2_SHEEP 53 22 -
NSKLSRMLTFKFYMPK O77620 53 22 -
NPKLSKILTFKINMPK IL2_HORSE 53 22 -
NPQLSRMLTFKFYTPK IL2_CANFA 54 23 -
NLKLSRMHTFNFYMPK IL2_CAPHI 53 22 -
NLKLSKMHTFNFFMPK IL2_CEREL 53 22 -
NPKLPMLLTFKFYMPR IL2_MERUN 53 22 -
NLKLPMMLTFKFYLPK IL2_RAT 53 22 -
NLKLPRMLTFKFYLPK IL2_MOUSE 67 36 -
NPRLPKMLKLKLYPPK O88210 51 20 -

Motif 3 width=28
Element Seqn Id St Int Rpt
ATELKHLQCLEEELKPLEEVLNLAQSKN IL2_HUMAN 70 1 -
ATELKHLQCLEEELKPLEEVLNLAQSKN IL2_MACMU 70 1 -
ATELKQLQCLEEELKPLEEVLNLAQSKN Q13169 73 1 -
ATELKHLQCLEEELKPLEEVLNLAQSKN IL2_CERTO 70 1 -
ATELRHLQCLEEELKPLEEVLNLAQSKS IL2_MACFA 70 1 -
ATELTHLQCLVEELKPLEEVLYLAQSKN IL2_FELCA 70 1 -
ATELKHLQCLVEELKALEGVLNLGQSKN IL2_PIG 70 1 -
ATELTHLQCLAEELKPLEEVLYLAQSKN IL2_MIRAN 70 1 -
ATELKHLKCLLEELKLLEEVLNLAPSKN IL2_BOVIN 71 2 -
ATELKHLKCLLEELKLLEEVLDLAPSKN IL2_SHEEP 71 2 -
VTELKHLQCLEEELKPLEEVLNLAQGKN O77620 70 1 -
ATELKHLQCLEEELKPLEEMLKNFLSKD IL2_HORSE 70 1 -
ATEFTHLQCLAEELKNLEEVLGLPQSKN IL2_CANFA 71 1 -
DTELKHLKCLLEELKLLEDVLDLAPSKN IL2_CAPHI 71 2 -
ATELKHLNCLLEELKLLEDVLSLSPSKN IL2_CEREL 71 2 -
ATELKHLQCLEEELGPLHDVLNLVQSKN IL2_MERUN 70 1 -
ATELKHLQCLENELGALQRVLDLTQSKS IL2_RAT 70 1 -
ATELKDLQCLEDELGPLRHVLDLTQSKS IL2_MOUSE 84 1 -
VSELQHLQCLEEELRAVEQVLNLAEHKN O88210 68 1 -

Motif 4 width=22
Element Seqn Id St Int Rpt
NINVIVLELKGSETTFMCEYAD IL2_HUMAN 108 10 -
NINVIVLELKGSETTLMCEYAD IL2_MACMU 109 11 -
NINVIVLELKGSETTFMCEYAD Q13169 111 10 -
NINVIVLELKGSETTLMCEYAD IL2_CERTO 109 11 -
NINVIVLELKGSETTLMCEYAD IL2_MACFA 109 11 -
NINVTVLKLKGSETRFTCNYDD IL2_FELCA 109 11 -
NINVTVLELKGSETSFKCEYDD IL2_PIG 109 11 -
NINVTLLKLKGSETRFKCEYDD IL2_MIRAN 109 11 -
NIKRIVLELQGSETRFTCEYDD IL2_BOVIN 110 11 -
NIKRIVLELQGSETRFTCEYDD IL2_SHEEP 110 11 -
NINVTVLKLKGSETFMCEYDET O77620 109 11 -
NINVTVLGLKGSETRFTCEYDN IL2_HORSE 104 6 -
NMNVTLLKLKGSETSYNCEYDD IL2_CANFA 110 11 -
SLKGIVLELQGSETRFTCEYDD IL2_CAPHI 110 11 -
NIKRIVLELQGSETSFKCEYDA IL2_CEREL 117 18 -
NIRVTVMKLKGSENTLNCEFDD IL2_MERUN 109 11 -
NIRVTVVKLKGSENKFECQFDD IL2_RAT 109 11 -
NIRVTVVKLKGSDNTFECQFDD IL2_MOUSE 123 11 -
NINVTVLSLKGSETAFVCDLED O88210 107 11 -

Motif 5 width=22
Element Seqn Id St Int Rpt
TATIVEFLNRWITFCQSIISTL IL2_HUMAN 131 1 -
TATIVEFLNRWITFCQSIISTL IL2_MACMU 132 1 -
TATIVEFLNRWITFCQSIISTL Q13169 134 1 -
TATIVEFLNRWITFCQSIISTL IL2_CERTO 132 1 -
TATIVEFLNRWITFCQSIISTL IL2_MACFA 132 1 -
TATIVEFLNKWITFCQSIFSTL IL2_FELCA 132 1 -
TVTAVEFLNKWITFCQSIYSTL IL2_PIG 132 1 -
TATITEFLNKWITFCQSIFSTL IL2_MIRAN 132 1 -
TVNAVEFLNKWITFCQSIYSTM IL2_BOVIN 133 1 -
TVKAVEFLNKWITFCQSIYSTM IL2_SHEEP 133 1 -
TVTIVEFLNRWITFCQSIISAS O77620 130 -1 -
TETIVEFLNKWITFCQSIISTL IL2_HORSE 127 1 -
TATITEFLNKWITFCQSIFSTL IL2_CANFA 133 1 -
TVKAVEFQNKWTTFCQSIYSTL IL2_CAPHI 133 1 -
TVKAVEFLNKWITFCQRIYSTM IL2_CEREL 140 1 -
TVTVVEFLSRWITFCQSAISTM IL2_MERUN 132 1 -
PATVVEFLRRWIAICQSIISTM IL2_RAT 132 1 -
SATVVDFLRRWIAFCQSIISTS IL2_MOUSE 146 1 -
SVNIVEFLKRWTAFCQKIMSRL O88210 130 1 -