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PR00168

Identifier
KCNECHANNEL  [View Relations]  [View Alignment]  
Accession
PR00168
No. of Motifs
3
Creation Date
19-MAR-1993  (UPDATE 01-DEC-2009)
Title
KCNE voltage-gated K+ channel family E signature
Database References
PRINTS; PR01604 KCNE1CHANNEL; PR01605 KCNE2CHANNEL; PR01606 KCNE3CHANNEL

PFAM; PF02060 ISK_Channel
INTERPRO; IPR000369
Literature References
1. MILLER, C.
An overview of the potassium channel family.
GENOME BIOL. 1(4) 1-5 (2000).
 
2. CONLEY, E.C. AND BRAMMAR, W.J.
MinK.
IN THE ION CHANNEL FACTSBOOK, VOLUME IV, ACADEMIC PRESS, 1999, PP.703-767.

Documentation
Potassium ion (K+) channels are a structurally diverse group of proteins
that facilitate the flow of K+ ions across cell membranes. They are
ubiquitous, being present in virtually all cell types. Activation of K+
channels tends to hyperpolarise cells, reducing the membrane's electrical
resistance, dampening nervous activity. In eukaryotic cells, K+ channels
are involved in neural signalling and generation of the cardiac rhythm, and
act as effectors in signal transduction pathways involving G protein-
coupled receptors (GPCRs). In prokaryotic cells, they play a role in the
maintenance of ionic homeostasis [1].
 
Some types of K+ channel are closed at the resting potential of the cell,
but open on membrane depolarisation, and are thus known as voltage-gated 
channels. Each of these types of channel typically comprises 4 pore-forming
alpha subunits that may associate with one of a number of different types of
beta subunit. Two types of beta subunit (KCNE and KCNAB) are presently known
to associate with voltage-gated alpha subunits (Kv, KCNQ and eag-like).
However, not all combinations of alpha and beta subunits are possible.
The KCNE family of K+ channel subunits are membrane glycoproteins that 
possess a single transmembrane (TM) domain. They share no structural 
relationship with the alpha subunit proteins, which possess pore forming 
domains. The subunits appear to have a regulatory function, modulating the 
kinetics and voltage dependence of the alpha subunits of voltage-dependent
K+ channels [2]. KCNE subunits are formed from short polypeptides of ~130
amino acids, and are divided into five subfamilies: KCNE1 (MinK/IsK),
KCNE2 (MiRP1), KCNE3 (MiRP2), KCNE4 (MiRP3) and KCNE1L (AMMECR2).
 
KCNECHANNEL is a 3-element fingerprint that provides a signature for the
KCNE voltage-gated K+ channel family E. The fingerprint was derived from an
initial alignment of 9 sequences: the motifs were drawn from conserved 
regions spannning the central portion of the alignment: motifs 1 and 2 span
the TM domain; and motif 3 spans the C-terminus of the TM domain and the
N-terminus of the cytoplasmic tail. Two iterations on SPTR39.22_17.3f were
required to reach convergence, at which point a true set comprising 14
sequences was identified.
 
An update on SPTR57_40f identified a true set of 18 sequences. Two partial  
matches were also found, Q8AWZ9_XENLA and Q8AWZ8_XENLA, which are frog 
translated mRNA sequences that fail to make a significant match to motif 1.
Summary Information
  18 codes involving  3 elements
2 codes involving 2 elements
Composite Feature Index
3181818
2022
123
True Positives
A7LFK5_RABIT  B4E2L9_HUMAN  KCNE1_PONAB   MINK_FELCA    
MINK_HUMAN MINK_MOUSE MINK_PIG MINK_RAT
MIR2_HUMAN MIR2_MOUSE MIR2_RAT Q28705
Q2KHV0_BOVIN Q545H6_MOUSE Q545H9_MOUSE Q60409
Q6IAE6_HUMAN Q8MIS1_PIG
True Positive Partials
Codes involving 2 elements
Q8AWZ8_XENLA Q8AWZ9_XENLA
Sequence Titles
A7LFK5_RABIT Potassium voltage-gated channel subfamily E member - Oryctolagus cuniculus (Rabbit). 
B4E2L9_HUMAN cDNA FLJ61204, highly similar to Potassium voltage-gated channel subfamily E member 1 - Homo sapiens (Human).
KCNE1_PONAB Potassium voltage-gated channel subfamily E member 1 - Pongo abelii (Sumatran orangutan).
MINK_FELCA ISK SLOW VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN (MINIMAL POTASSIUM CHANNEL) (MINK) - Felis silvestris catus (Cat).
MINK_HUMAN ISK SLOW VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN (MINIMAL POTASSIUM CHANNEL) (MINK) - Homo sapiens (Human).
MINK_MOUSE ISK SLOW VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN (MINIMAL POTASSIUM CHANNEL) (MINK) - Mus musculus (Mouse).
MINK_PIG ISK SLOW VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN (MINIMAL POTASSIUM CHANNEL) (MINK) - Sus scrofa (Pig).
MINK_RAT ISK SLOW VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN (MINIMAL POTASSIUM CHANNEL) (MINK) - Rattus norvegicus (Rat).
MIR2_HUMAN MINIMUM POTASSIUM ION CHANNEL-RELATED PEPTIDE 2 (MIRP2) (MINK-RELATED PEPTIDE 2) - Homo sapiens (Human).
MIR2_MOUSE MINIMUM POTASSIUM ION CHANNEL-RELATED PEPTIDE 2 (MIRP2) (MINK-RELATED PEPTIDE 2) - Mus musculus (Mouse).
MIR2_RAT MINIMUM POTASSIUM ION CHANNEL-RELATED PEPTIDE 2 (MIRP2) (MINK-RELATED PEPTIDE 2) - Rattus norvegicus (Rat).
Q28705 CARDIAC DELAYED RECTIFIER POTASSIUM CHANNEL PROTEIN - ORYCTOLAGUS CUNICULUS (RABBIT).
Q2KHV0_BOVIN Potassium voltage-gated channel subfamily E member - Bos taurus (Bovine).
Q545H6_MOUSE Potassium voltage-gated channel subfamily E member - Mus musculus (Mouse).
Q545H9_MOUSE Potassium voltage-gated channel subfamily E member - Mus musculus (Mouse).
Q60409 POTASSIUM CHANNEL - CAVIA PORCELLUS (GUINEA PIG).
Q6IAE6_HUMAN Potassium voltage-gated channel subfamily E member - Homo sapiens (Human).
Q8MIS1_PIG Potassium voltage-gated channel subfamily E member - Sus scrofa (Pig).

Q8AWZ8_XENLA Voltage-gated potassium channel subunit MiRP2 (KCNE3 protein) - Xenopus laevis (African clawed frog).
Q8AWZ9_XENLA Potassium voltage-gated channel subfamily E member - Xenopus laevis (African clawed frog).
Scan History
SPTR39.22_17.3f 2  210  NSINGLE    
SPTR57_40f 3 250 NSINGLE
Initial Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
EALYILMVL MINK_CAVPO 43 43 -
EALYILMVL MINK_FELCA 43 43 -
EALYVLMVL MINK_HUMAN 43 43 -
VILYLMVMI MIR1_HUMAN 49 49 -
VILYLMVMI MIR1_RAT 49 49 -
SYMYILFVM MIR2_HUMAN 57 57 -
SYMYILFVM MIR2_MOUSE 57 57 -
SYMYILFVM MIR2_RAT 61 61 -
EYFYILVVM MIR3_MOUSE 35 35 -

Motif 2 width=12
Element Seqn Id St Int Rpt
GFFGFFTLGIML MINK_CAVPO 52 0 -
GFFGFFTLGIML MINK_FELCA 52 0 -
GFFGFFTLGIML MINK_HUMAN 52 0 -
GMFSFIIVAILV MIR1_HUMAN 58 0 -
GMFAFIVVAILV MIR1_RAT 58 0 -
FLFAVTVGSLIL MIR2_HUMAN 66 0 -
FLFAVTVGSLIL MIR2_MOUSE 66 0 -
FLFAVTVGSLIL MIR2_RAT 70 0 -
SFYGVFLIGIML MIR3_MOUSE 44 0 -

Motif 3 width=9
Element Seqn Id St Int Rpt
SYIRSKKLE MINK_CAVPO 64 0 -
SYIRSKKLE MINK_FELCA 64 0 -
SYIRSKKLE MINK_HUMAN 64 0 -
STVKSKRRE MIR1_HUMAN 70 0 -
STVKSKRRE MIR1_RAT 70 0 -
GYTRSRKVD MIR2_HUMAN 78 0 -
GYTRSRKVD MIR2_MOUSE 78 0 -
GYTRSRKVD MIR2_RAT 82 0 -
GYMKSKRRE MIR3_MOUSE 56 0 -
Final Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
EALYILMVL Q60409 43 43 -
EALYILMVL Q545H6_MOUSE 43 43 -
EALYILMVL MINK_RAT 44 44 -
EALYILMVL MINK_PIG 44 44 -
EALYILMVL MINK_MOUSE 43 43 -
EALYILMVL MINK_FELCA 43 43 -
EALYILMVL A7LFK5_RABIT 44 44 -
EALYILMVL Q28705 44 44 -
AALYILMVL Q2KHV0_BOVIN 43 43 -
EALYVLMVL B4E2L9_HUMAN 17 17 -
EALYVLMVL KCNE1_PONAB 43 43 -
EALYVLMVL MINK_HUMAN 43 43 -
SYMYILFVM MIR2_HUMAN 57 57 -
SYMYILFVM Q6IAE6_HUMAN 57 57 -
SYMYILFVM Q545H9_MOUSE 57 57 -
SYMYILFVM MIR2_RAT 61 61 -
SYMYILFVM MIR2_MOUSE 57 57 -
SYMYILFVM Q8MIS1_PIG 53 53 -

Motif 2 width=12
Element Seqn Id St Int Rpt
GFFGFFTLGIML Q60409 52 0 -
GFFGFFTLGIML Q545H6_MOUSE 52 0 -
GFFGFFTLGIML MINK_RAT 53 0 -
GFFGFFTLGIML MINK_PIG 53 0 -
GFFGFFTLGIML MINK_MOUSE 52 0 -
GFFGFFTLGIML MINK_FELCA 52 0 -
GFFGFFTLGIML A7LFK5_RABIT 53 0 -
GFFGFFTLGIML Q28705 53 0 -
GFFGFFTLGIML Q2KHV0_BOVIN 52 0 -
GFFGFFTLGIML B4E2L9_HUMAN 26 0 -
GFFGFFTLGIML KCNE1_PONAB 52 0 -
GFFGFFTLGIML MINK_HUMAN 52 0 -
FLFAVTVGSLIL MIR2_HUMAN 66 0 -
FLFAVTVGSLIL Q6IAE6_HUMAN 66 0 -
FLFAVTVGSLIL Q545H9_MOUSE 66 0 -
FLFAVTVGSLIL MIR2_RAT 70 0 -
FLFAVTVGSLIL MIR2_MOUSE 66 0 -
FLFAATVGSLIL Q8MIS1_PIG 62 0 -

Motif 3 width=9
Element Seqn Id St Int Rpt
SYIRSKKLE Q60409 64 0 -
SYIRSKKLE Q545H6_MOUSE 64 0 -
SYIRSKKLE MINK_RAT 65 0 -
SYIRSKKLE MINK_PIG 65 0 -
SYIRSKKLE MINK_MOUSE 64 0 -
SYIRSKKLE MINK_FELCA 64 0 -
SYIRSQKLE A7LFK5_RABIT 65 0 -
SYIRSQKLE Q28705 65 0 -
SYIRSKKLE Q2KHV0_BOVIN 64 0 -
SYIRSKKLE B4E2L9_HUMAN 38 0 -
SYIRSKKLE KCNE1_PONAB 64 0 -
SYIRSKKLE MINK_HUMAN 64 0 -
GYTRSRKVD MIR2_HUMAN 78 0 -
GYTRSRKVD Q6IAE6_HUMAN 78 0 -
GYTRSRKVD Q545H9_MOUSE 78 0 -
GYTRSRKVD MIR2_RAT 82 0 -
GYTRSRKVD MIR2_MOUSE 78 0 -
GYTRSRKVD Q8MIS1_PIG 74 0 -