| Literature References | 1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
3. GONZALEZ-CANDELAS, L., RAMON, D. AND POLIANA, J.
Sequences and homology analysis of 2 genes encoding beta-glucosidases
from Bacillus polymyxa.
GENE 95 31-38 (1990).
4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
Nucleotide sequences of the Arb genes, which control beta-glucosidase
utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
coli Bgl operon and evidence for a new beta-glycohydrolase family
including enzymes from eubacteria, archaebacteria and humans.
J.BACTERIOL. 174 765-777 (1992).
5. WITHERS, S.G., WARREN, R.A.J., STREET, I.P., RUPITZ, K., KEMPTON, J.B.
AND AEBERSOLD, R.
Unequivocal demonstration of the involvement of a glutamate residue as
a nucleophile in the mechanism of a retaining glycosidase.
J.AM.CHEM.SOC. 112 5887-5889 (1990).
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| Documentation | O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt).
Family 1 includes beta-glucosidases, beta-galactosidases, plant myrosinases,
mammalian lactase-phlorizin hydrolase, and so on. Alignments of such
sequences reveal a number of short conserved regions, one of which contains
a glutamic acid residue, which has been shown to be involved in glycosidic
bond cleavage in beta-glucosidase from Agrobacterium [5].
GLHYDRLASE1 is a 5-element fingerprint that provides a signature for
family 1 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved regions in
the second half of the alignment up to the C-terminus, motif 2 including
the region encoded by PROSITE pattern GLYCOSYL_HYDROLASE_F1_1 (PS00572),
which contains the catalytically active Glu. Three iterations on OWL24.0
were required to reach convergence, at which point a true set comprising
38 sequences was identified. Two partial matches were also found:
BGLS_TRIRP fails to make a significant match with motif 2 and is truncated
at motif 5; and BGLT_TRIRP is a fragment that matches only motifs 1 and 2.
An update on SPTR37_9f identified a true set of 75 sequences, and 11
partial matches.
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| Sequence Titles | ABGA_CLOLO 6-PHOSPHO-BETA-GLUCOSIDASE (EC 3.2.1.86) - CLOSTRIDIUM LONGISPORUM.
ARBB_ERWCH 6-PHOSPHO-BETA-GLUCOSIDASE (EC 3.2.1.86) - ERWINIA CHRYSANTHEMI.
ASCB_ECOLI 6-PHOSPHO-BETA-GLUCOSIDASE ASCB (EC 3.2.1.86) - ESCHERICHIA COLI.
BGAL_SULAC BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - SULFOLOBUS ACIDOCALDARIUS.
BGAL_SULSH BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - SULFOLOBUS SHIBATAE.
BGAL_SULSO BETA-GALACTOSIDASE (EC 3.2.1.23) (LACTASE) - SULFOLOBUS SOLFATARICUS.
BGL1_BACSU BETA-GLUCOSIDASE (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D- GLUCOSIDE GLUCOHYDROLASE) (AMYGDALASE) - BACILLUS SUBTILIS.
BGL2_BACSU PROBABLE BETA-GLUCOSIDASE (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D-GLUCOSIDE GLUCOHYDROLASE) (AMYGDALASE) - BACILLUS SUBTILIS.
BGLA_BACCI BETA-GLUCOSIDASE (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D- GLUCOSIDE GLUCOHYDROLASE) (AMYGDALASE) - BACILLUS CIRCULANS.
BGLA_BACSU 6-PHOSPHO-BETA-GLUCOSIDASE (EC 3.2.1.86) - BACILLUS SUBTILIS.
BGLA_CLOTM BETA-GLUCOSIDASE A (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D- GLUCOSIDE GLUCOHYDROLASE) - CLOSTRIDIUM THERMOCELLUM.
BGLA_ECOLI 6-PHOSPHO-BETA-GLUCOSIDASE BGLA (EC 3.2.1.86) - ESCHERICHIA COLI.
BGLA_ERWHE BETA-GLUCOSIDASE A (EC 3.2.1.21) (GENTIOBIASE) (BETA-D-GLUCOSIDE GLUCOHYDROLASE) - ERWINIA HERBICOLA.
BGLA_PAEPO BETA-GLUCOSIDASE A (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D- GLUCOSIDE GLUCOHYDROLASE) (AMYGDALASE) (BGA) - PAENIBACILLUS POLYMYXA (BACILLUS POLYMYXA).
BGLA_THEMA BETA-GLUCOSIDASE A (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D- GLUCOSIDE GLUCOHYDROLASE) - THERMOTOGA MARITIMA.
BGLB_ECOLI 6-PHOSPHO-BETA-GLUCOSIDASE BGLB (EC 3.2.1.86) - ESCHERICHIA COLI.
BGLB_MICBI THERMOSTABLE BETA-GLUCOSIDASE B (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D-GLUCOSIDE GLUCOHYDROLASE) (AMYGDALASE) - MICROBISPORA BISPORA.
BGLB_PAEPO BETA-GLUCOSIDASE B (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D- GLUCOSIDE GLUCOHYDROLASE) (AMYGDALASE) - PAENIBACILLUS POLYMYXA (BACILLUS POLYMYXA).
BGLC_MAIZE BETA-GLUCOSIDASE, CHLOROPLAST PRECURSOR (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D-GLUCOSIDE GLUCOHYDROLASE) - ZEA MAYS (MAIZE).
BGLS_AGRSP BETA-GLUCOSIDASE (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D- GLUCOSIDE GLUCOHYDROLASE) (AMYGDALASE) - AGROBACTERIUM SP. (STRAIN ATCC 21400).
BGLS_CALSA BETA-GLUCOSIDASE A (EC 3.2.1.21) (GENTIOBIASE) (CELLOBIASE) (BETA-D- GLUCOSIDE GLUCOHYDROLASE) (AMYGDALASE) - CALDOCELLUM SACCHAROLYTICUM (CALDICELLULOSIRUPTOR SACCHAROLYTICUS).
CASB_KLEOX PHOSPHO-CELLOBIASE (EC 3.2.1.-) - KLEBSIELLA OXYTOCA.
LACG_LACAC 6-PHOSPHO-BETA-GALACTOSIDASE (EC 3.2.1.85) (BETA-D-PHOSPHOGALACTOSIDE GALACTOHYDROLASE) (PGALASE) (P-BETA-GAL) (PBG) - LACTOBACILLUS ACIDOPHILUS.
LACG_LACCA 6-PHOSPHO-BETA-GALACTOSIDASE (EC 3.2.1.85) (BETA-D-PHOSPHOGALACTOSIDE GALACTOHYDROLASE) (PGALASE) (P-BETA-GAL) (PBG) - LACTOBACILLUS CASEI.
LACG_LACLA 6-PHOSPHO-BETA-GALACTOSIDASE (EC 3.2.1.85) (BETA-D-PHOSPHOGALACTOSIDE GALACTOHYDROLASE) (PGALASE) (P-BETA-GAL) (PBG) - LACTOCOCCUS LACTIS (SUBSP. LACTIS) (STREPTOCOCCUS LACTIS).
LACG_STAAU 6-PHOSPHO-BETA-GALACTOSIDASE (EC 3.2.1.85) (BETA-D-PHOSPHOGALACTOSIDE GALACTOHYDROLASE) (PGALASE) (P-BETA-GAL) (PBG) - STAPHYLOCOCCUS AUREUS.
LACG_STRMU 6-PHOSPHO-BETA-GALACTOSIDASE (EC 3.2.1.85) (BETA-D-PHOSPHOGALACTOSIDE GALACTOHYDROLASE) (PGALASE) (P-BETA-GAL) (PBG) - STREPTOCOCCUS MUTANS.
LPH_HUMAN LACTASE-PHLORIZIN HYDROLASE PRECURSOR (EC 3.2.1.108) (EC 3.2.1.62) (LACTASE-GLYCOSYLCERAMIDASE) - HOMO SAPIENS (HUMAN).
LPH_RABIT LACTASE-PHLORIZIN HYDROLASE PRECURSOR (EC 3.2.1.108) (EC 3.2.1.62) (LACTASE-GLYCOSYLCERAMIDASE) (LPH) - ORYCTOLAGUS CUNICULUS (RABBIT).
MYR3_SINAL MYROSINASE MB3 PRECURSOR (EC 3.2.3.1) (SINIGRINASE) (THIOGLUCOSIDASE) - SINAPIS ALBA (WHITE MUSTARD) (BRASSICA HIRTA).
MYRO_ARATH MYROSINASE PRECURSOR (EC 3.2.3.1) (SINIGRINASE) (THIOGLUCOSIDASE) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
MYRO_BRANA MYROSINASE PRECURSOR (EC 3.2.3.1) (SINIGRINASE) (THIOGLUCOSIDASE) - BRASSICA NAPUS (RAPE).
O05508 C. THERMOCELLUM BETA-GLUCOSIDASE - BACILLUS SUBTILIS.
O06532 PHOSPHO-BETA-GALACTOSIDASE 1 - LACTOBACILLUS GASSERI.
O08324 BETA-GLUCOSIDASE (EC 3.2.1.21) (GENTOBIASE) (CELLOBIASE) (AMYGDALASE) - THERMOCOCCUS SP. (STRAIN 9ON-7).
O23656 BETA-GLUCOSIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O24433 BETA-GLUCOSIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O24524 LINAMARASE (EC 3.2.1.21) (BETA-GLUCOSIDASE) (GENTOBIASE) (CELLOBIASE) (AMYGDALASE) - MANIHOT ESCULENTA (CASSAVA) (MANIOC).
O32287 BETA-GLUCOSIDASE (EC 3.2.1.21) - BACILLUS SUBTILIS.
O48779 PUTATIVE BETA-GLUCOSIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O49117 ATA27 - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O50658 PHOSPHO-BETA-GALACTOSIDASE II - LACTOBACILLUS GASSERI.
O52629 BETA-GALACTOSIDASE - PYROCOCCUS WOESEI.
O58104 423AA LONG HYPOTHETICAL BETA-GLUCOSIDASE - PYROCOCCUS HORIKOSHII.
O58237 483AA LONG HYPOTHETICAL BETA-MANNOSIDASE - PYROCOCCUS HORIKOSHII.
O61594 BETA-GLUCOSIDASE PRECURSOR - SPODOPTERA FRUGIPERDA (FALL ARMYWORM).
O64879 PUTATIVE BETA-GLUCOSIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O64880 PUTATIVE BETA-GLUCOSIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O64881 PUTATIVE BETA-GLUCOSIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O64882 PUTATIVE BETA-GLUCOSIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O64883 PUTATIVE BETA-GLUCOSIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O65458 GLUCOSIDASE LIKE PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O80689 F8K4.2 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O80690 F8K4.3 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O80750 T13D8.16 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O86291 BETA-GLUCOSIDASE - LACTOBACILLUS PLANTARUM.
P94248 BETA-D-GLUCOSIDASE (EC 3.2.1.21) (BETA-GLUCOSIDASE) (GENTOBIASE) (CELLOBIASE) (AMYGDALASE) - BIFIDOBACTERIUM BREVE.
Q18758 COSMID C50F7 - CAENORHABDITIS ELEGANS.
Q38786 BETA-D-GLUCOSIDASE - AVENA SATIVA (OAT).
Q39343 MYROSINASE PRECURSOR - BRASSICA NAPUS (RAPE).
Q40283 BETA GLUCOSIDASE PRECURSOR - MANIHOT ESCULENTA (CASSAVA) (MANIOC).
Q41172 LINAMARASE - MANIHOT ESCULENTA (CASSAVA) (MANIOC).
Q41290 BETA-GLUCOSIDASE, CHLOROPLAST PRECURSOR (EC 3.2.1.21) (DHURRINASE) - SORGHUM BICOLOR MILO (SORGHUM).
Q41761 BETA-D-GLUCOSIDASE PRECURSOR - ZEA MAYS (MAIZE).
Q42585 THIOGLUCOSIDE GLUCOHYDROLASE PRECURSOR (EC 3.2.3.1) (THIOGLUCOSIDASE) (MYROSINASE) (SINIGRINASE) (SINIGRASE) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q42595 THIOGLUCOSIDASE (EC 3.2.3.1) (MYROSINASE) (SINIGRINASE) (SINIGRASE) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q42618 BETA-GLUCOSIDASE (EC 3.2.1.21) (GENTOBIASE) (CELLOBIASE) (AMYGDALASE) - BRASSICA NAPUS (RAPE).
Q42629 MYROSINASE, THIOGLUCOSIDE GLUCOHYDROLASE PRECURSOR (EC 3.2.3.1) (THIOGLUCOSIDASE) (SINIGRINASE) (SINIGRASE) - BRASSICA NAPUS (RAPE).
Q42707 FUROSTANOL GLYCOSIDE 26-O-BETA-GLUCOSIDASE (F26G) (EC 3.2.1.21) - COSTUS SPECIOSUS.
Q43073 PRUNASIN HYDROLASE PRECURSOR (EC 3.2.1.21) (BETA-GLUCOSIDASE) (GENTOBIASE) (CELLOBIASE) (AMYGDALASE) - PRUNUS SEROTINA (BLACK CHERRY).
Q51723 BETA-GLUCOSIDASE - PYROCOCCUS FURIOSUS.
Q51733 BETA-MANNOSIDASE - PYROCOCCUS FURIOSUS.
Q55000 BETA-GLUCOSIDASE - STREPTOMYCES ROCHEI (STREPTOMYCES PARVULLUS).
Q59976 BETA-GLUCOSIDASE (EC 3.2.1.21) (GENTOBIASE) (CELLOBIASE) (AMYGDALASE) - STREPTOMYCES SP.
Q60026 BETA-GLUCOSIDASE (EC 3.2.1.21) (GENTOBIASE) (CELLOBIASE) (AMYGDALASE) - THERMOANAEROBACTER BROCKII (THERMOANAEROBIUM BROCKII).
BGLS_TRIRP NON-CYANOGENIC BETA-GLUCOSIDASE PRECURSOR (EC 3.2.1.21) - TRIFOLIUM REPENS (CREEPING WHITE CLOVER).
O80749 T13D8.15 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O82772 BETA-GLUCOSIDASE HOMOLOG - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
P97265 CYTOSOLIC BETA-GLUCOSIDASE - CAVIA PORCELLUS (GUINEA PIG).
Q40025 BETA-GLUCOSIDASE - HORDEUM VULGARE (BARLEY).
Q42975 BETA GLUCOSIDASE (EC 3.2.1.21) (BETA-GLUCOSIDASE) (GENTOBIASE) (CELLOBIASE) (AMYGDALASE) - ORYZA SATIVA (RICE).
Q46043 BETA-GLUCOSIDASE - CELLULOMONAS FIMI.
O35082 KLOTHO - MUS MUSCULUS (MOUSE).
O70175 KLOTHO - MUS MUSCULUS (MOUSE).
O70621 SECRETED ISOFORM OF KLOTHO PROTEIN - MUS MUSCULUS (MOUSE).
Q12601 BETA-GLUCOSIDASE PRECURSOR (EC 3.2.1.21) (GENTOBIASE) (CELLOBIASE) (AMYGDALASE) - CANDIDA WICKERHAMII.
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