| Literature References | 1. MULLER, M.M., RUPPERT, S., SCHAFFNER, W. AND MATTHIAS, P.
A cloned octamer transcription factor stimulates transcription from
lymphoid-specific promoters in non-B cells.
NATURE 336 544-551 (1988).
2. SCHEIDEREIT, C., CROMLISH, J.A., GERSTER, T., KAWAKAMI, K.,
BALMACEDA, C.-G., CURRIE, R.A. AND RODER, R.G.
A human lymphoid-specific transcription factor that activates
immunoglobulin genes is a homeobox protein.
NATURE 336 551-557 (1988).
3. DEKKER, N., COX, M., BOELENS, R., VERRIJZER, C.P., VAN DER VLIET, P.C.
AND KAPTEIN, R.
Solution structure of the POU-specific DNA-binding domain of OCT-1.
NATURE 362 852-855 (1993).
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| Documentation | The octamer-binding protein is a transcription factor that binds
specifically to the octamer motif (ATTTGCAT) of immunoglobulin promoters
and activates these genes. There are two Ig octamer-binding proteins,
designated NF-A1 and NF-A2: NF-A1 is found in all cell types, while
NF-A2 is found only in lymphoid cells. The sequences of these proteins
contain a region that correponds closely to the DNA-binding homeobox
domains and POU transcription factors.
OCTAMER is a 4-element fingerprint that provides a signature for both the
NF-A1 class of nuclear RNA and histone transcription factors, and the
NF-A2 class of lymphoid-restricted immunoglobulin octamer-binding proteins.
The fingerprint was derived from an initial alignment of 6 sequences: the
motifs were drawn from conserved, glutamine-rich regions, motif 3
corresponding to the inhibitory domain and motif 4 falling at the start of
the POU domain. Two iterations on OWL22.1 were required to reach
convergence, at which point a true set comprising 23 sequences was
identified. Several partial matches were also found, of which three are
POU sequences that match motifs 3 and 4 (PDM2_DROME, A46196 and DRODPOU28).
An update on SPTR37_9f identified a true set of 15 sequences, and 9
partial matches.
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