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PR02000

Identifier
GCR1PLANT  [View Relations]  [View Alignment]  
Accession
PR02000
No. of Motifs
11
Creation Date
20-APR-2009
Title
Putative plant GPCR, GCR1, signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR02001 GCR1CAMPR

PFAM; PF05462 Dicty_CAR
INTERPRO; IPR000848
Literature References
1. JOSEFSSON,L.G. AND RASK, L.
Cloning of a putative G-protein-coupled receptor from Arabidopsis thaliana.
EUR.J.BIOCHEM. 249 415-420 (1997).
 
2. PLAKIDOU-DYMOCK, S., DYMOCK, D. AND HOOLEY, R.
A higher plant seven-transmembrane receptor that influences sensitivity to
cytokinins.
CURR.BIOL. 8 315-324 (1998).

Documentation
Heterotrimeric G protein-mediated signalling via activated 7-transmembrane
(7TM) serpentine receptors is well known in a range of organisms, but 
evidence for the existence of this pathway in plants has been elusive.
Over the years, however, evidence that G proteins are involved in signal 
transduction in plants has been accumulating, leading several researchers
to seek plant 7TM receptors.
 
The first tangible evidence for a plant 7TM receptor came through an
analysis of the expresssed sequence tag database, dbEST. This revealed 
an Arabidopsis thaliana gene (GCR1) that encodes a protein with 7 predicted
TM domains, and other features characteristic of known 7TM receptors [1,2].
The amino acid sequence of GCR1, however, is unusual in that it shows
similarity to receptors from three different 7TM families [1,2], sharing
greatest levels of similarity with the Dictyostelium cAMP receptors.
 
GCR1 is expressed at low levels in the roots, stems and leaves of
Arabidopsis. Studies using transgenic Arabidopsis have suggested a 
functional role for GCR1 in cytokinin signal transduction [2].
 
GCR1PLANT is an 11-element fingerprint that provides a signature for the
putative plant GPCR, GCR1. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved regions
spanning the full alignment length. Two iterations on SPTR55_38f were
required to reach convergence, at which point a true set comprising 12
sequences was identified. Two partial matches were also found, both of 
which are related proteins that fail to make significant matches with
2 or more motifs.
Summary Information
  12 codes involving 11 elements
0 codes involving 10 elements
1 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
111212121212121212121212
1000000000000
911111100111
800000000000
700000000000
600000000000
500000000000
400000000000
300000000000
200000000101
1234567891011
True Positives
A9SH56_PHYPA  A9U1I7_PHYPA  B6GV49_LOTJA  B6SHE2_MAIZE  
B8B3R3_ORYSI B9HMQ6_POPTR B9S014_RICCO O04214_ARATH
O04714_ARATH Q4ZH52_PEA Q69K53_ORYSJ Q8LPG0_ARATH
True Positive Partials
Codes involving 9 elements
A7P4V2_VITVI
Codes involving 2 elements
Q22X36_TETTH
Sequence Titles
A9SH56_PHYPA Predicted protein; - Physcomitrella patens subsp. patens. 
A9U1I7_PHYPA Predicted protein; - Physcomitrella patens subsp. patens.
B6GV49_LOTJA Putative G-protein coupled receptor; - Lotus japonicus.
B6SHE2_MAIZE G protein-coupled receptor; - Zea mays (Maize).
B8B3R3_ORYSI Putative uncharacterized protein; - Oryza sativa subsp. indica (Rice).
B9HMQ6_POPTR Predicted protein; - Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa).
B9S014_RICCO Cyclic AMP receptor, putative; - Ricinus communis (Castor bean).
O04214_ARATH Putative G protein coupled receptor; - Arabidopsis thaliana (Mouse-ear cress).
O04714_ARATH Putative G-protein-coupled receptor (F11A17.17 protein); - Arabidopsis thaliana (Mouse-ear cress).
Q4ZH52_PEA G-protein coupled receptor; - Pisum sativum (Garden pea).
Q69K53_ORYSJ Os06g0199800 protein; Putative uncharacterized protein; cDNA clone:J033046K12, full insert sequence; G protein coupled receptor-related-like; - Oryza sativa subsp. japonica (Rice).
Q8LPG0_ARATH Putative G protein-coupled receptor; - Arabidopsis thaliana (Mouse-ear cress).

A7P4V2_VITVI Chromosome chr4 scaffold_6, whole genome shotgun sequence; - Vitis vinifera (Grape).

Q22X36_TETTH 7 transmembrane receptor secretin family protein; - Tetrahymena thermophila SB210.
Scan History
SPTR55_38f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
ITAINTGASSLSFVGS O04214_ARATH 19 19 -
ITAINTGASSLSFVGS O04714_ARATH 19 19 -
ITAINTGASSLSFVGS Q8LPG0_ARATH 19 19 -
LDAVGTSAAALSLAGS Q69K53_ORYSJ 21 21 -
ITAINTGASSLSFVGS Q4ZH52_PEA 19 19 -
LTVINQCASALSFVGT A9SH56_PHYPA 13 13 -
LTVINQCASALSFIGT A9U1I7_PHYPA 13 13 -

Motif 2 width=15
Element Seqn Id St Int Rpt
FIVLCYCLFKELRKF O04214_ARATH 36 1 -
FIVLCYCLFKELRKF O04714_ARATH 36 1 -
FIVLCYCLFKELRKF Q8LPG0_ARATH 36 1 -
FIVLCYLLFRELRKF Q69K53_ORYSJ 38 1 -
FIVLCYCIYKDINKY Q4ZH52_PEA 36 1 -
FIVMCYVLFKDLRSF A9SH56_PHYPA 30 1 -
FIVMCYVLFKDLRSF A9U1I7_PHYPA 30 1 -

Motif 3 width=23
Element Seqn Id St Int Rpt
FKLVFYLALSDMLCSFFLIVGDP O04214_ARATH 52 1 -
FKLVFYLALSDMLCSFFLIVGDP O04714_ARATH 52 1 -
FKLVFYLALSDVLCSFFLIVGDP Q8LPG0_ARATH 52 1 -
FKLVYFLAVSDMFCSLFTIMGGP Q69K53_ORYSJ 54 1 -
FKLVFYLALSDMLCSFFLIVGDP Q4ZH52_PEA 52 1 -
FKLVFYLSLSDMLASLFNMLGNP A9SH56_PHYPA 46 1 -
FKLVFYLSLSDMLASLFNMLGNP A9U1I7_PHYPA 46 1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
AQGYTTHFFCVASF O04214_ARATH 82 7 -
AQGYTTHFFCVASF O04714_ARATH 82 7 -
AQGYTTHFFCVASF Q8LPG0_ARATH 82 7 -
AHDYSAHFFCVASF Q69K53_ORYSJ 84 7 -
AQGYTTHFFCVASF Q4ZH52_PEA 82 7 -
AQGYSSQFFSIASF A9SH56_PHYPA 76 7 -
AQGYSSQFFSIASF A9U1I7_PHYPA 76 7 -

Motif 5 width=19
Element Seqn Id St Int Rpt
WTTTIAFTLHRTVVKHKTD O04214_ARATH 97 1 -
WTTTIAFTLHRTVVKHKTD O04714_ARATH 97 1 -
WTTTIAFTLHRTVVKHKTD Q8LPG0_ARATH 97 1 -
WTTTIAFTLHRTVVKHKTD Q69K53_ORYSJ 99 1 -
WTTTIAFTLHRTILKHKSQ Q4ZH52_PEA 97 1 -
WTTTISFTLHRAAVKHKTD A9SH56_PHYPA 91 1 -
WTTTISFTLHRAAVKHKTD A9U1I7_PHYPA 91 1 -

Motif 6 width=16
Element Seqn Id St Int Rpt
EAMFHLYVWGTSLVVT O04214_ARATH 120 4 -
EAMFHLYVWGTSLVVT O04714_ARATH 120 4 -
EAMFHLYVWGTSLVVT Q8LPG0_ARATH 120 4 -
GSIFHLYVWGTSLATT Q69K53_ORYSJ 122 4 -
EAMFHLYVWGTSLVVT Q4ZH52_PEA 120 4 -
GAIFHVYVWGTSLMMT A9SH56_PHYPA 114 4 -
GAIFHAYVWGTSLVMT A9U1I7_PHYPA 114 4 -

Motif 7 width=13
Element Seqn Id St Int Rpt
GKAVHFLTFYAPL O04214_ARATH 159 23 -
GKAVHFLTFYAPL O04714_ARATH 159 23 -
GKAVHFLTFYAPL Q8LPG0_ARATH 159 23 -
GKVLHLVTFYLPL Q69K53_ORYSJ 161 23 -
AKAVHFLTFYAPL Q4ZH52_PEA 159 23 -
GKVLRFMTFYLPL A9SH56_PHYPA 154 24 -
GKVLRFMTFYLPL A9U1I7_PHYPA 154 24 -

Motif 8 width=24
Element Seqn Id St Int Rpt
WGAILYNGFTYFQVIRMLRNARRM O04214_ARATH 172 0 -
WGAILYNGFTYFQVIRMLRNARRM O04714_ARATH 172 0 -
WGAILYNGFTYFQVIRMLRNARRM Q8LPG0_ARATH 172 0 -
WGAILYNGYTYFQVNRMINNATRM Q69K53_ORYSJ 174 0 -
WGAILYNGFTYFDLLEMIEQGNNM Q4ZH52_PEA 172 0 -
WGAILFNGIAYFQVIRVINNATSM A9SH56_PHYPA 167 0 -
WGAILFNGIAYFQIIRVINNATSM A9U1I7_PHYPA 167 0 -

Motif 9 width=22
Element Seqn Id St Int Rpt
LNRWGYYPLILIGSWAFGTINR O04214_ARATH 215 19 -
LNRWGYYPLILIGSWAFGTINR O04714_ARATH 215 19 -
LNRWGYYPLILIGSWAFGTINR Q8LPG0_ARATH 215 19 -
FNRWGYYPLILIGSWAFATINR Q69K53_ORYSJ 217 19 -
LNRWGYYPLILIGSWAFGTINR Q4ZH52_PEA 215 19 -
LNRYGYYPLILIGSFTFGTINR A9SH56_PHYPA 210 19 -
LNRYGYYPLILIGSFTFGTINR A9U1I7_PHYPA 215 24 -

Motif 10 width=18
Element Seqn Id St Int Rpt
HKIFWLSVLDVGTAALMG O04214_ARATH 245 8 -
HKIFWLSVLDVGTAALMG O04714_ARATH 245 8 -
HKIFWLSVLDVGTAALMG Q8LPG0_ARATH 245 8 -
HKIFWLSILDVGFAGLMG Q69K53_ORYSJ 247 8 -
HKIFWLSVLDVGTAALMG Q4ZH52_PEA 245 8 -
HPLFWLYCLDIASASLMG A9SH56_PHYPA 240 8 -
HPLFWLYCLDIASASLMG A9U1I7_PHYPA 245 8 -

Motif 11 width=17
Element Seqn Id St Int Rpt
LFNSIAYGFNSSVRRAI O04214_ARATH 263 0 -
LFNSIAYGFNSSVRRAI O04714_ARATH 263 0 -
LFNSIAYGFNSSVRRAI Q8LPG0_ARATH 263 0 -
LFNSIAYGLNSSVRRAI Q69K53_ORYSJ 265 0 -
LFNSIAYAFRTSLNNGL Q4ZH52_PEA 263 0 -
FFNSIAYGYNSSVRRVL A9SH56_PHYPA 258 0 -
FFNSIAYGYNNSVRRVL A9U1I7_PHYPA 263 0 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
ITAINTGASSLSFVGS O04214_ARATH 19 19 -
ITAINTGASSLSFVGS O04714_ARATH 19 19 -
ITAINTGASSLSFVGS Q8LPG0_ARATH 19 19 -
LTAVNAGASSLSLAGS B6GV49_LOTJA 19 19 -
LTSVNAGASSLSFVGS B9S014_RICCO 18 18 -
LMAVKGGASSLSFLGS B9HMQ6_POPTR 15 15 -
LDAVGTSAAALSLAGS B8B3R3_ORYSI 21 21 -
LDAVGTSAAALSLAGS Q69K53_ORYSJ 21 21 -
LDAVGTGAAALSLAGS B6SHE2_MAIZE 23 23 -
ITAINTGASSLSFVGS Q4ZH52_PEA 19 19 -
LTVINQCASALSFVGT A9SH56_PHYPA 13 13 -
LTVINQCASALSFIGT A9U1I7_PHYPA 13 13 -

Motif 2 width=15
Element Seqn Id St Int Rpt
FIVLCYCLFKELRKF O04214_ARATH 36 1 -
FIVLCYCLFKELRKF O04714_ARATH 36 1 -
FIVLCYCLFKELRKF Q8LPG0_ARATH 36 1 -
FIVLCYLLFKELRKF B6GV49_LOTJA 36 1 -
FIVLCYVLFKELRKF B9S014_RICCO 35 1 -
FIVLCYLLFKELRKF B9HMQ6_POPTR 32 1 -
FIVLCYLLFRELRKF B8B3R3_ORYSI 38 1 -
FIVLCYLLFRELRKF Q69K53_ORYSJ 38 1 -
FIVICYLLFRELRKF B6SHE2_MAIZE 40 1 -
FIVLCYCIYKDINKY Q4ZH52_PEA 36 1 -
FIVMCYVLFKDLRSF A9SH56_PHYPA 30 1 -
FIVMCYVLFKDLRSF A9U1I7_PHYPA 30 1 -

Motif 3 width=23
Element Seqn Id St Int Rpt
FKLVFYLALSDMLCSFFLIVGDP O04214_ARATH 52 1 -
FKLVFYLALSDMLCSFFLIVGDP O04714_ARATH 52 1 -
FKLVFYLALSDVLCSFFLIVGDP Q8LPG0_ARATH 52 1 -
FKLVFYLALSDMLCSFFNVIGDP B6GV49_LOTJA 52 1 -
FKLVFYLALSDMLCSFFSIVGDP B9S014_RICCO 51 1 -
FKLVFYLALSDMLCSFFSIVGDP B9HMQ6_POPTR 48 1 -
FKLVYFLAVSDMFCSLFTIMGGP B8B3R3_ORYSI 54 1 -
FKLVYFLAVSDMFCSLFTIMGGP Q69K53_ORYSJ 54 1 -
FKLVFYLAVSDMFCSLFTILGGP B6SHE2_MAIZE 56 1 -
FKLVFYLALSDMLCSFFLIVGDP Q4ZH52_PEA 52 1 -
FKLVFYLSLSDMLASLFNMLGNP A9SH56_PHYPA 46 1 -
FKLVFYLSLSDMLASLFNMLGNP A9U1I7_PHYPA 46 1 -

Motif 4 width=14
Element Seqn Id St Int Rpt
AQGYTTHFFCVASF O04214_ARATH 82 7 -
AQGYTTHFFCVASF O04714_ARATH 82 7 -
AQGYTTHFFCVASF Q8LPG0_ARATH 82 7 -
AQGYSTHFFCVASF B6GV49_LOTJA 82 7 -
AQGYTTHFFCVASF B9S014_RICCO 81 7 -
AQGYSTHFFCVASF B9HMQ6_POPTR 78 7 -
AHDYSAHFFCVASF B8B3R3_ORYSI 84 7 -
AHDYSAHFFCVASF Q69K53_ORYSJ 84 7 -
AHDYSAHFFCVASF B6SHE2_MAIZE 86 7 -
AQGYTTHFFCVASF Q4ZH52_PEA 82 7 -
AQGYSSQFFSIASF A9SH56_PHYPA 76 7 -
AQGYSSQFFSIASF A9U1I7_PHYPA 76 7 -

Motif 5 width=19
Element Seqn Id St Int Rpt
WTTTIAFTLHRTVVKHKTD O04214_ARATH 97 1 -
WTTTIAFTLHRTVVKHKTD O04714_ARATH 97 1 -
WTTTIAFTLHRTVVKHKTD Q8LPG0_ARATH 97 1 -
WTTTIAFTLHRTVVKHKTD B6GV49_LOTJA 97 1 -
WTTTIAFTLHRTVVLHKTD B9S014_RICCO 96 1 -
WTTTIAFTLHRTVVKHKTD B9HMQ6_POPTR 93 1 -
WTTTIAFTLHRTVVKHKTD B8B3R3_ORYSI 99 1 -
WTTTIAFTLHRTVVKHKTD Q69K53_ORYSJ 99 1 -
WTTTISFTLHRTVVKHKTD B6SHE2_MAIZE 101 1 -
WTTTIAFTLHRTILKHKSQ Q4ZH52_PEA 97 1 -
WTTTISFTLHRAAVKHKTD A9SH56_PHYPA 91 1 -
WTTTISFTLHRAAVKHKTD A9U1I7_PHYPA 91 1 -

Motif 6 width=16
Element Seqn Id St Int Rpt
EAMFHLYVWGTSLVVT O04214_ARATH 120 4 -
EAMFHLYVWGTSLVVT O04714_ARATH 120 4 -
EAMFHLYVWGTSLVVT Q8LPG0_ARATH 120 4 -
EAMFHLYVWGTSLVMT B6GV49_LOTJA 120 4 -
EAIFHLYVWGTSLVMT B9S014_RICCO 119 4 -
EAMFHLYVWGTSLFLT B9HMQ6_POPTR 116 4 -
GSIFHLYVWGTSLATT B8B3R3_ORYSI 122 4 -
GSIFHLYVWGTSLATT Q69K53_ORYSJ 122 4 -
GSIFHLYVWGTSLSTT B6SHE2_MAIZE 124 4 -
EAMFHLYVWGTSLVVT Q4ZH52_PEA 120 4 -
GAIFHVYVWGTSLMMT A9SH56_PHYPA 114 4 -
GAIFHAYVWGTSLVMT A9U1I7_PHYPA 114 4 -

Motif 7 width=13
Element Seqn Id St Int Rpt
GKAVHFLTFYAPL O04214_ARATH 159 23 -
GKAVHFLTFYAPL O04714_ARATH 159 23 -
GKAVHFLTFYAPL Q8LPG0_ARATH 159 23 -
GKAIHFITFYMPL B6GV49_LOTJA 159 23 -
RKAIQFITFYAPL B9S014_RICCO 159 24 -
RKAVQFITFYAPL B9HMQ6_POPTR 155 23 -
GKVLHLVTFYLPL B8B3R3_ORYSI 161 23 -
GKVLHLVTFYLPL Q69K53_ORYSJ 161 23 -
AKVLHLITFYLPL B6SHE2_MAIZE 163 23 -
AKAVHFLTFYAPL Q4ZH52_PEA 159 23 -
GKVLRFMTFYLPL A9SH56_PHYPA 154 24 -
GKVLRFMTFYLPL A9U1I7_PHYPA 154 24 -

Motif 8 width=24
Element Seqn Id St Int Rpt
WGAILYNGFTYFQVIRMLRNARRM O04214_ARATH 172 0 -
WGAILYNGFTYFQVIRMLRNARRM O04714_ARATH 172 0 -
WGAILYNGFTYFQVIRMLRNARRM Q8LPG0_ARATH 172 0 -
WGAILYNGFTYLQVIRMLNNATRM B6GV49_LOTJA 172 0 -
WGAILYNGFTYFQVIRMLNNATRM B9S014_RICCO 172 0 -
WGAILYNGFTYYQVIRMLNNATRM B9HMQ6_POPTR 168 0 -
WGAILYNGYTYFQVNRMINNATRM B8B3R3_ORYSI 174 0 -
WGAILYNGYTYFQVNRMINNATRM Q69K53_ORYSJ 174 0 -
WGAILYNGFTYYEVNRMLNNATQM B6SHE2_MAIZE 176 0 -
WGAILYNGFTYFDLLEMIEQGNNM Q4ZH52_PEA 172 0 -
WGAILFNGIAYFQVIRVINNATSM A9SH56_PHYPA 167 0 -
WGAILFNGIAYFQIIRVINNATSM A9U1I7_PHYPA 167 0 -

Motif 9 width=22
Element Seqn Id St Int Rpt
LNRWGYYPLILIGSWAFGTINR O04214_ARATH 215 19 -
LNRWGYYPLILIGSWAFGTINR O04714_ARATH 215 19 -
LNRWGYYPLILIGSWAFGTINR Q8LPG0_ARATH 215 19 -
LNRWGYYPLILIGSWAFGTINR B6GV49_LOTJA 215 19 -
LNRWGYYPLILIGSWAFGTINR B9S014_RICCO 215 19 -
MNRWGYYPLILIGSWAFGTING B9HMQ6_POPTR 211 19 -
FNRWGYYPLILIGSWAFATINR B8B3R3_ORYSI 217 19 -
FNRWGYYPLILIGSWAFATINR Q69K53_ORYSJ 217 19 -
FNRWGYYPLILIGSWAFATINR B6SHE2_MAIZE 219 19 -
LNRWGYYPLILIGSWAFGTINR Q4ZH52_PEA 215 19 -
LNRYGYYPLILIGSFTFGTINR A9SH56_PHYPA 210 19 -
LNRYGYYPLILIGSFTFGTINR A9U1I7_PHYPA 215 24 -

Motif 10 width=18
Element Seqn Id St Int Rpt
HKIFWLSVLDVGTAALMG O04214_ARATH 245 8 -
HKIFWLSVLDVGTAALMG O04714_ARATH 245 8 -
HKIFWLSVLDVGTAALMG Q8LPG0_ARATH 245 8 -
HKIFWLSLLDVGTAALMG B6GV49_LOTJA 245 8 -
HKILWLSVLDVGTAALMG B9S014_RICCO 245 8 -
HKIFWLSVLDVGTAALMG B9HMQ6_POPTR 241 8 -
HKIFWLSILDVGFAGLMG B8B3R3_ORYSI 247 8 -
HKIFWLSILDVGFAGLMG Q69K53_ORYSJ 247 8 -
HRIVWLSFLDVGFAGLMG B6SHE2_MAIZE 249 8 -
HKIFWLSVLDVGTAALMG Q4ZH52_PEA 245 8 -
HPLFWLYCLDIASASLMG A9SH56_PHYPA 240 8 -
HPLFWLYCLDIASASLMG A9U1I7_PHYPA 245 8 -

Motif 11 width=17
Element Seqn Id St Int Rpt
LFNSIAYGFNSSVRRAI O04214_ARATH 263 0 -
LFNSIAYGFNSSVRRAI O04714_ARATH 263 0 -
LFNSIAYGFNSSVRRAI Q8LPG0_ARATH 263 0 -
LFNSIAYGLNSSVRRAI B6GV49_LOTJA 263 0 -
LFNSIAYGLNTSVRRAI B9S014_RICCO 263 0 -
LSNSIAYGLNSSVRRAI B9HMQ6_POPTR 259 0 -
LFNSIAYGLNSSVRRAI B8B3R3_ORYSI 265 0 -
LFNSIAYGLNSSVRRAI Q69K53_ORYSJ 265 0 -
LFNSIAYGLNSSVRRAI B6SHE2_MAIZE 267 0 -
LFNSIAYAFRTSLNNGL Q4ZH52_PEA 263 0 -
FFNSIAYGYNSSVRRVL A9SH56_PHYPA 258 0 -
FFNSIAYGYNNSVRRVL A9U1I7_PHYPA 263 0 -