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PR01909

Identifier
ADSPHPHTASEA  [View Relations]  [View Alignment]  
Accession
PR01909
No. of Motifs
4
Creation Date
11-MAY-2006
Title
Atypical dual specificity phosphatase subfamily A signature 
Database References
PRINTS; PR00700 PRTYPHPHTASE; PR01764 MAPKPHPHTASE; PR01908 ADSPHPHTASE

INTERPRO; IPR000340; IPR000387
PDB; 1J4X; 1VHR
SCOP; 1J4X; 1VHR
CATH; 1J4X; 1VHR
Literature References
1. ISHIBASHI, T., BOTTARO, D.P., CHAN, A., MIKI, T. AND AARONSON, S.A. 
Expression cloning of a human dual-specificity phosphatase. 
PROC.NATL.ACAD.SCI.U.S.A. 89 12170-12174 (1992). 
 
2. YUVANIYAMA, J., DENU, J.M., DIXON, J.E. AND SAPER, M.A. 
Crystal structure of the dual specificity protein phosphatase VHR. 
SCIENCE 272 1328-1331 (1996). 
 
3. TODD, J.L., TANNER, K.G. AND DENU, J.M. 
Extracellular regulated kinases (ERK) 1 and ERK2 are authentic substrates 
for the dual-specificity protein-tyrosine phosphatase VHR. A novel role in 
down-regulating the ERK pathway.
J.BIOL.CHEM. 274(19) 13271-80 (1999).
 
4. TODD, J.L., RIGAS, J.D., RAFTY, L.A. AND DENU, J.M. 
Dual-specificity protein tyrosine phosphatase VHR down-regulates c-Jun 
N-terminal kinase (JNK).
ONCOGENE 21(16) 2573-83 (2002).
 
5. VASUDEVAN, S.A., SKOKO, J., WANG, K., BURLINGAME, S.M., PATEL, P.N., 
LAZO, J.S., NUCHTERN, J.G. AND YANG, J. 
MKP-8, a novel MAPK phosphatase that inhibits p38 kinase.
BIOCHEM.BIOPHYS.RES.COMMUN. 330(2) 511-8 (2005).
 
6. RAHMOUNI, S., CERIGNOLI, F., ALONSO, A., TSUTJI, T., HENKENS, R., 
ZHU, C., LOUIS-DIT-SULLY, C., MOUTSCHEN, M., JIANG, W. AND MUSTELIN, T. 
Loss of the VHR dual-specific phosphatase causes cell-cycle arrest and
senescence.
NAT.CELL BIOL. 8(5) 524-31 (2006). 

Documentation
Dual Specificity Phosphatases (DUSPs) are members of the superfamily of 
Protein Tyrosine Phosphatases (PTPs). They remove the phosphate group from
both phospho-tyrosine and phospho-serine/threonine residues. Many of these
proteins have been related to the MAP kinase signalling pathway and hence they
are also termed MAP kinase phosphatases (MKPs). A group of DUSPs share a high
degree of similarity with the MKP subgroup, but lack the N-terminal regulatory
domain, which provides the substrate specificity towards the MAP kinases.
These proteins form a heterogeneous group and have in common the presence of
a single catalytic PTP domain. They are small-sized and have been named Low
Molecular Weight-DUSPs (LMW-DUSPs) or Atypical-DUSPs. VHR was the first
characterised member of this subfamily; its crystal structure is known [1,2].
 
The functions of many of the members of this group remain unknown, although 
some have been related to regulation of MAP kinase pathways [3-5]. VHR has
also been related to the control of cell-senescence [6]. On the basis of
sequence similarity, this family can be subdivided into two groups, for
convenience termed A and B. Each of these subgroups is characterised by its
own distinctive set of motifs, the functions of which are as yet unknown. 
 
ADSPHPHTASEA is a 4-element fingerprint that provides a signature for the
atypical dual specificity phosphatase subfamily A. The fingerprint was derived
from an initial alignment of 12 sequences: the motifs were drawn from conserved
regions spanning the N-terminal portion of the alignment, avoiding the catalytic
PTP domain, which is conserved in the PTP superfamily. Two iterations were 
required to reach convergence, at which point a true set comprising 25 sequences
was identified. Several partial matches were also found, representing orthologous
DUSPs from different organisms.
Summary Information
  25 codes involving  4 elements
4 codes involving 3 elements
4 codes involving 2 elements
Composite Feature Index
425252525
33441
22321
1234
True Positives
DUS13_HUMAN   DUS13_MOUSE   DUS3_HUMAN    DUS3_MOUSE    
DUS3_PONPY Q25N50_MACFA Q2T9T7_BOVIN Q497R2_MOUSE
Q4RQC9_TETNG Q4RQD0_TETNG Q4S3F5_TETNG Q5FVI9_RAT
Q5R6H6_PONPY Q5U0J1_HUMAN Q5XIN2_RAT Q68J44_HUMAN
Q6B8I0_MOUSE Q6B8I1_HUMAN Q6IAR0_HUMAN Q7TNW3_MOUSE
Q8BK84_MOUSE Q96J67_HUMAN Q9BV47_HUMAN Q9D700_MOUSE
Q9DA25_MOUSE
True Positive Partials
Codes involving 3 elements
Q29RA3_BRARE Q3UQN8_MOUSE Q4S3F6_TETNG Q8IYJ9_HUMAN
Codes involving 2 elements
Q3V2Y9_MOUSE Q5VZP5_HUMAN Q8SXF8_DROME Q9VWN2_DROME
Sequence Titles
DUS13_HUMAN Dual specificity protein phosphatase 13 (EC 3.1.3.48) (EC 3.1.3.16) (Testis- and skeletal-muscle-specific DSP) (Dual specificity phosphatase SKRP4) - Homo sapiens (Human). 
DUS13_MOUSE Dual specificity protein phosphatase 13 (EC 3.1.3.48) (EC 3.1.3.16) (Testis- and skeletal muscle-specific DSP) (Dual specificity tyrosine phosphatase TS-DSP6) - Mus musculus (Mouse).
DUS3_HUMAN Dual specificity protein phosphatase 3 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase VHR) - Homo sapiens (Human).
DUS3_MOUSE Dual specificity protein phosphatase 3 (EC 3.1.3.48) (EC 3.1.3.16) (T- DSP11) - Mus musculus (Mouse).
DUS3_PONPY Dual specificity protein phosphatase 3 (EC 3.1.3.48) (EC 3.1.3.16) - Pongo pygmaeus (Orangutan).
Q25N50_MACFA Brain cDNA clone: QorA-11778, similar to human hypothetical protein MGC1136 (MGC1136), - Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey).
Q2T9T7_BOVIN Hypothetical protein - Bos taurus (Bovine).
Q497R2_MOUSE Dusp13 protein - Mus musculus (Mouse).
Q4RQC9_TETNG Chromosome 17 SCAF15006, whole genome shotgun sequence - Tetraodon nigroviridis (Green puffer).
Q4RQD0_TETNG Chromosome 17 SCAF15006, whole genome shotgun sequence - Tetraodon nigroviridis (Green puffer).
Q4S3F5_TETNG Chromosome 2 SCAF14750, whole genome shotgun sequence - Tetraodon nigroviridis (Green puffer).
Q5FVI9_RAT Hypothetical protein RGD1310090 - Rattus norvegicus (Rat).
Q5R6H6_PONPY Hypothetical protein DKFZp468D1919 - Pongo pygmaeus (Orangutan).
Q5U0J1_HUMAN Dual specificity phosphatase 3 (Vaccinia virus phosphatase VH1- related) - Homo sapiens (Human).
Q5XIN2_RAT Dual specificity phosphatase 13 - Rattus norvegicus (Rat).
Q68J44_HUMAN FMDSP - Homo sapiens (Human).
Q6B8I0_MOUSE Muscle restricted dual specificity phosphatase - Mus musculus (Mouse).
Q6B8I1_HUMAN Muscle restricted dual specificity phosphatase - Homo sapiens (Human).
Q6IAR0_HUMAN DUSP13 protein - Homo sapiens (Human).
Q7TNW3_MOUSE Dsp13-like protein - Mus musculus (Mouse).
Q8BK84_MOUSE 18-day embryo whole body cDNA, RIKEN full-length enriched library, clone:1110056B09 product:weakly similar to DUAL SPECIFICITY PROTEIN PHOSPHATASE 13 (EC 3.1.3.48) (EC 3.1.3.16) (TESTIS- AND SKELETAL- MUSCLE-SPECIFIC DSP) (Similar to human protein phosphatase) - Mus musculus (Mouse).
Q96J67_HUMAN Branching-enzyme interacting dual-specificity protein phosphatase BEDP - Homo sapiens (Human).
Q9BV47_HUMAN Dual specificity phosphatase 26 (Low-molecular-mass dual-specificity phosphatase 4) (Dual-specificity phosphatase SKRP3) (Mitogen-activated protein kinase phosphatase 8) (NATA1 protein) (CDNA FLJ31142 fis, clone IMR322001317, weakly similar to Homo sapiens protein phosphatase) - Homo sapiens (Human).
Q9D700_MOUSE Adult male tongue cDNA, RIKEN full-length enriched library, clone:2310043K02 product:HYPOTHETICAL 23.9 kDa PROTEIN (CDNA FLJ31142 FIS, CLONE IMR322001317, WEAKLY SIMILAR TO HOMO SAPIENS PROTEIN PHOSPHATASE) homolog (Dusp26 protein) (Dual-specificity phosphatase SKRP3) - Mus musculus (Mouse).
Q9DA25_MOUSE Adult male testis cDNA, RIKEN full-length enriched library, clone:1700022L10 product:dual specificity phosphatase 13, full insert sequence - Mus musculus (Mouse).

Q29RA3_BRARE Hypothetical protein - Brachydanio rerio (Zebrafish) (Danio rerio).
Q3UQN8_MOUSE 13 days embryo heart cDNA, RIKEN full-length enriched library, clone:D330024O22 product:hypothetical Dual specificity protein phosphatase containing protein, full insert sequence - Mus musculus (Mouse).
Q4S3F6_TETNG Chromosome 2 SCAF14750, whole genome shotgun sequence - Tetraodon nigroviridis (Green puffer).
Q8IYJ9_HUMAN DUSP3 protein - Homo sapiens (Human).

Q3V2Y9_MOUSE 16 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:9630035G15 product:dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related), full insert sequence - Mus musculus (Mouse).
Q5VZP5_HUMAN Novel protein - Homo sapiens (Human).
Q8SXF8_DROME RH25447p - Drosophila melanogaster (Fruit fly).
Q9VWN2_DROME CG7378-PA - Drosophila melanogaster (Fruit fly).
Scan History
SPTR49_32f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
DEVWPGLYLGDQ Q5FVI9_RAT 63 63 -
DEVWPGLYLGDQ Q9D700_MOUSE 63 63 -
DEVWPGLYLGDQ Q9BV47_HUMAN 63 63 -
DEVWPGLYLGDQ Q5R6H6_PONPY 63 63 -
DEVWPNLFIGDA Q96J67_HUMAN 39 39 -
NEVWPNLFLGDA DUS13_MOUSE 47 47 -
NEVWPNLFLGDA Q5XIN2_RAT 47 47 -
DEVWPSLFLGDA DUS13_HUMAN 47 47 -
NEVTPRIYVGNA DUS3_HUMAN 31 31 -
NEVTPRIYVGNA DUS3_PONPY 31 31 -
NEVWPKLYIGDE Q68J44_HUMAN 56 56 -
NEVVPRVYVGNA DUS3_MOUSE 31 31 -

Motif 2 width=10
Element Seqn Id St Int Rpt
LRRLGITHVL Q5FVI9_RAT 83 8 -
LRRLGITHVL Q9D700_MOUSE 83 8 -
LRRLGITHVL Q9BV47_HUMAN 83 8 -
LRRLGITHVL Q5R6H6_PONPY 83 8 -
LWKLGITHVL Q96J67_HUMAN 59 8 -
LIQLGITHVV DUS13_MOUSE 67 8 -
LIQLGITHVV Q5XIN2_RAT 67 8 -
LIQLGITHVV DUS13_HUMAN 67 8 -
LQKLGITHVL DUS3_HUMAN 51 8 -
LQKLGITHVL DUS3_PONPY 51 8 -
LQKAGFTHVL Q68J44_HUMAN 76 8 -
LQKLGITHVL DUS3_MOUSE 51 8 -

Motif 3 width=12
Element Seqn Id St Int Rpt
YLGVEAHDSPAF Q5FVI9_RAT 113 20 -
YLGVEAHDSPAF Q9D700_MOUSE 113 20 -
YLGVEAHDSPAF Q9BV47_HUMAN 113 20 -
YLGVEAHDSPAF Q5R6H6_PONPY 113 20 -
YLGVPAHDLPDF Q96J67_HUMAN 90 21 -
YYGIEADDNPFF DUS13_MOUSE 99 22 -
YYGIEADDNPFF Q5XIN2_RAT 99 22 -
YYGIEADDNPFF DUS13_HUMAN 99 22 -
YLGIKANDTQEF DUS3_HUMAN 85 24 -
YLGIKANDTQEF DUS3_PONPY 85 24 -
YHGVEADDLPTF Q68J44_HUMAN 108 22 -
YLGIKANDTQEF DUS3_MOUSE 85 24 -

Motif 4 width=10
Element Seqn Id St Int Rpt
AADFIHRALS Q5FVI9_RAT 133 8 -
AADFIHRALS Q9D700_MOUSE 133 8 -
AADFIHRALS Q9BV47_HUMAN 133 8 -
AADFIHRALS Q5R6H6_PONPY 133 8 -
AADFIHRALN Q96J67_HUMAN 110 8 -
VARYIRDALN DUS13_MOUSE 119 8 -
VARYIRDALN Q5XIN2_RAT 119 8 -
VARYIRAALS DUS13_HUMAN 119 8 -
AADFIDQALA DUS3_HUMAN 105 8 -
AADFIDQALA DUS3_PONPY 105 8 -
AAAFIDRALS Q68J44_HUMAN 128 8 -
ATDFIDQALA DUS3_MOUSE 105 8 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
DEVWPGLYLGDQ Q5FVI9_RAT 63 63 -
DEVWPGLYLGDQ Q9D700_MOUSE 63 63 -
DEVWPGLYLGDQ Q9BV47_HUMAN 63 63 -
DEVWPGLYLGDQ Q5R6H6_PONPY 63 63 -
DEVWPNLFIGDA Q6B8I1_HUMAN 39 39 -
DEVWPNLFIGDA Q96J67_HUMAN 39 39 -
DEVWPNLFIGDA Q6B8I0_MOUSE 39 39 -
DEVWPGLYLGDQ Q25N50_MACFA 63 63 -
NEVWPNLFLGDA DUS13_MOUSE 47 47 -
NEVWPNLFLGDA Q9DA25_MOUSE 112 112 -
NEVWPNLFLGDA Q7TNW3_MOUSE 112 112 -
NEVWPNLFLGDA Q5XIN2_RAT 47 47 -
NEVWPNLFLGDA Q497R2_MOUSE 47 47 -
DEVWPSLFLGDA DUS13_HUMAN 47 47 -
DEVWPSLFLGDA Q6IAR0_HUMAN 47 47 -
NEVTPRIYVGNA DUS3_HUMAN 31 31 -
NEVTPRIYVGNA Q5U0J1_HUMAN 31 31 -
NEVTPRIYVGNA Q2T9T7_BOVIN 31 31 -
NEVTPRIYVGNA DUS3_PONPY 31 31 -
DEVWPRLYLGDM Q4RQC9_TETNG 72 72 -
DEVWPKIYIGNA Q4S3F5_TETNG 33 33 -
NEVWPKLYIGDE Q68J44_HUMAN 56 56 -
NEVVPRVYVGNA DUS3_MOUSE 31 31 -
DEVWPNLYIGNV Q4RQD0_TETNG 31 31 -
NEVWPRLHIGDE Q8BK84_MOUSE 55 55 -

Motif 2 width=10
Element Seqn Id St Int Rpt
LRRLGITHVL Q5FVI9_RAT 83 8 -
LRRLGITHVL Q9D700_MOUSE 83 8 -
LRRLGITHVL Q9BV47_HUMAN 83 8 -
LRRLGITHVL Q5R6H6_PONPY 83 8 -
LWKLGITHVL Q6B8I1_HUMAN 59 8 -
LWKLGITHVL Q96J67_HUMAN 59 8 -
LWKLGITHVL Q6B8I0_MOUSE 59 8 -
LRRLGITHVL Q25N50_MACFA 83 8 -
LIQLGITHVV DUS13_MOUSE 67 8 -
LIQLGITHVV Q9DA25_MOUSE 132 8 -
LIQLGITHVV Q7TNW3_MOUSE 132 8 -
LIQLGITHVV Q5XIN2_RAT 67 8 -
LIQLGITHVV Q497R2_MOUSE 67 8 -
LIQLGITHVV DUS13_HUMAN 67 8 -
LIQLGITHVV Q6IAR0_HUMAN 67 8 -
LQKLGITHVL DUS3_HUMAN 51 8 -
LQKLGITHVL Q5U0J1_HUMAN 51 8 -
LQKLGITHVL Q2T9T7_BOVIN 69 26 -
LQKLGITHVL DUS3_PONPY 51 8 -
LWQLGVTHVL Q4RQC9_TETNG 92 8 -
LRKLGITHVL Q4S3F5_TETNG 53 8 -
LQKAGFTHVL Q68J44_HUMAN 76 8 -
LQKLGITHVL DUS3_MOUSE 51 8 -
LHKLGITHVL Q4RQD0_TETNG 51 8 -
LQKAGFTHVL Q8BK84_MOUSE 75 8 -

Motif 3 width=12
Element Seqn Id St Int Rpt
YLGVEAHDSPAF Q5FVI9_RAT 113 20 -
YLGVEAHDSPAF Q9D700_MOUSE 113 20 -
YLGVEAHDSPAF Q9BV47_HUMAN 113 20 -
YLGVEAHDSPAF Q5R6H6_PONPY 113 20 -
YLGVPAHDLPDF Q6B8I1_HUMAN 90 21 -
YLGVPAHDLPDF Q96J67_HUMAN 90 21 -
YLGIPAHDLPDF Q6B8I0_MOUSE 90 21 -
SLGVEAHDSPAF Q25N50_MACFA 113 20 -
YYGIEADDNPFF DUS13_MOUSE 99 22 -
YYGIEADDNPFF Q9DA25_MOUSE 164 22 -
YYGIEADDNPFF Q7TNW3_MOUSE 164 22 -
YYGIEADDNPFF Q5XIN2_RAT 99 22 -
YYGIEADDNPFF Q497R2_MOUSE 99 22 -
YYGIEADDNPFF DUS13_HUMAN 99 22 -
YYGIEADDNPFF Q6IAR0_HUMAN 99 22 -
YLGIKANDTQEF DUS3_HUMAN 85 24 -
YLGIKANDTQEF Q5U0J1_HUMAN 85 24 -
YLGIKANDTQEF Q2T9T7_BOVIN 103 24 -
YLGIKANDTQEF DUS3_PONPY 85 24 -
YYGVPANDLPAF Q4RQC9_TETNG 123 21 -
YCGIPADDSTHF Q4S3F5_TETNG 84 21 -
YHGVEADDLPTF Q68J44_HUMAN 108 22 -
YLGIKANDTQEF DUS3_MOUSE 85 24 -
YLGIPAEDSESF Q4RQD0_TETNG 82 21 -
YHGVEADDVPTF Q8BK84_MOUSE 107 22 -

Motif 4 width=10
Element Seqn Id St Int Rpt
AADFIHRALS Q5FVI9_RAT 133 8 -
AADFIHRALS Q9D700_MOUSE 133 8 -
AADFIHRALS Q9BV47_HUMAN 133 8 -
AADFIHRALS Q5R6H6_PONPY 133 8 -
AADFIHRALN Q6B8I1_HUMAN 110 8 -
AADFIHRALN Q96J67_HUMAN 110 8 -
AADFIHRALT Q6B8I0_MOUSE 110 8 -
AADFIHRALS Q25N50_MACFA 133 8 -
VARYIRDALN DUS13_MOUSE 119 8 -
VARYIRDALN Q9DA25_MOUSE 184 8 -
VARYIRDALN Q7TNW3_MOUSE 184 8 -
VARYIRDALN Q5XIN2_RAT 119 8 -
VARYIRDALN Q497R2_MOUSE 119 8 -
VARYIRAALS DUS13_HUMAN 119 8 -
VARYIRAALS Q6IAR0_HUMAN 119 8 -
AADFIDQALA DUS3_HUMAN 105 8 -
AADFIDQALA Q5U0J1_HUMAN 105 8 -
AADFIDQALA Q2T9T7_BOVIN 123 8 -
AADFIDQALA DUS3_PONPY 105 8 -
AAEFIHQALT Q4RQC9_TETNG 143 8 -
AADFIHKALQ Q4S3F5_TETNG 104 8 -
AAAFIDRALS Q68J44_HUMAN 128 8 -
ATDFIDQALA DUS3_MOUSE 105 8 -
AVDFIHRVLK Q4RQD0_TETNG 102 8 -
AAAFIDSALR Q8BK84_MOUSE 127 8 -