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PR01894

Identifier
WNT14PROTEIN  [View Relations]  [View Alignment]  
Accession
PR01894
No. of Motifs
4
Creation Date
27-MAY-2003
Title
Wnt-14 protein signature
Database References
PRINTS; PR01349 WNTPROTEIN
MIM; 602863
Literature References
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
 
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
 
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.REV. 33 1-12 (2000).
 
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
 
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
 
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
 
7. BERGSTEIN, I., EISENBERG, L.M., BHALERAO, J., JENKINS, N.A.,
COPELAND, N.G., OSBOURNE, M.P., BOWCOCK, A.M. AND BROWN, A.M.
Isolation of two novel WNT genes, WNT14 and WNT15, one of which (WNT15) is 
closely linked to WNT3 on human chromosome 17q21.
GENOMICS 46 450-458 (1997).
 
8. KIRIKOSHI, H., SEKIHARA, H. AND KATOH, M.
Expression of WNT14 and WNT14B mRNAs in human cancer, up-regulation of WNT14
by IFNgamma and up-regulation of WNT14B by beta-estradiol.
INT.J.ONCOL. 19 1221-1225 (2001).

Documentation
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidney,
regulation of mammary gland proliferation, and Alzheimer's disease [3,4].
 
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of 
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been 
identified in vertebrates, with multiple subtypes within some classes.
 
Human Wnt-14 maps to chromosome 1 and originates, together with Wnt-15, from
an ancestral branch of the Wnt gene family, which includes the Wnt-9
sequences found in jawless and cartilaginous fishes [7]. Wnt-14 mRNA is 
preferentially expressed in various types of human cancer, e.g. gastric and 
pancreatic cancer [8].
 
WNT14PROTEIN is a 4-element fingerprint that provides a signature for the 
Wnt-14 proteins. The fingerprint was derived from an initial alignment of 2 
sequences: the motifs were drawn from conserved regions spanning the full
alignment length, focusing on those sections that characterise Wnt-14 
proteins but distinguish them from other Wnt subtypes - motifs 1-3 reside in 
short regions between conserved cysteines; and motif 4 includes conserved 
Cys15. Two iterations on SPTR40_22f were required to reach convergence, at 
which point a true set comprising 3 sequences was identified.
Summary Information
3 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
43333
30000
20000
1234
True Positives
Q8R5M2        WN14_CHICK    WN14_HUMAN    
Sequence Titles
Q8R5M2      Wnt14 - Mus musculus (Mouse).                 
WN14_CHICK WNT-14 protein precursor - Gallus gallus (Chicken).
WN14_HUMAN WNT-14 protein precursor - Homo sapiens (Human).
Scan History
SPTR40_22f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
PEAAAQAHYKA WN14_HUMAN 48 48 -
EEAAVKAHYKV WN14_CHICK 40 40 -

Motif 2 width=14
Element Seqn Id St Int Rpt
GVAETLVEAVSMSA WN14_HUMAN 77 18 -
GGAETLMEAISMSA WN14_CHICK 69 18 -

Motif 3 width=15
Element Seqn Id St Int Rpt
ASGAGGSDPLPRTPE WN14_HUMAN 274 183 -
KSIPGHSDQIPRTTD WN14_CHICK 263 180 -

Motif 4 width=10
Element Seqn Id St Int Rpt
HREKNCESIC WN14_HUMAN 314 25 -
YKDKNCDSIC WN14_CHICK 303 25 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
PEAAAQAHYKA WN14_HUMAN 48 48 -
TEAAAQAHYKA Q8R5M2 48 48 -
EEAAVKAHYKV WN14_CHICK 40 40 -

Motif 2 width=14
Element Seqn Id St Int Rpt
GVAETLVEAVSMSA WN14_HUMAN 77 18 -
GVAETLVEAVSMSA Q8R5M2 77 18 -
GGAETLMEAISMSA WN14_CHICK 69 18 -

Motif 3 width=15
Element Seqn Id St Int Rpt
ASGAGGSDPLPRTPE WN14_HUMAN 274 183 -
ASGSGGGDPLPRTPE Q8R5M2 274 183 -
KSIPGHSDQIPRTTD WN14_CHICK 263 180 -

Motif 4 width=10
Element Seqn Id St Int Rpt
HREKNCESIC WN14_HUMAN 314 25 -
HREKNCESIC Q8R5M2 314 25 -
YKDKNCDSIC WN14_CHICK 303 25 -