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PR01890

Identifier
PPNRPEPTIDEW  [View Relations]  [View Alignment]  
Accession
PR01890
No. of Motifs
4
Creation Date
23-FEB-2003
Title
Neuropeptide W precursor signature
Database References
PRINTS; PR01888 NROPEPTIDEBW
Literature References
1. SHIMOMURA, Y., HARADA, M., GOTO, M., SUGO, T., MATSUMOTO, Y., ABE, M.,
WATANABE, T., ASAMI, T., KITADA, C., MORI, M., ONDA, H. AND FUJINO, M.
Identification of neuropeptide W as the endogenous ligand for orphan
G-protein-coupled receptors GPR7 and GPR8.
J.BIOL.CHEM. 277(39) 35826-35832 (2002).
 
2. BREZILLON, S., LANNOY, V., FRANSSEN, J.D., LE POUL, E., DUPRIEZ, V.,
LUCCHETTI, J., DETHEUX, M. AND PARMENTIER, M.
Identification of natural ligands for the orphan G protein-coupled receptors
GPR7 and GPR8.
J.BIOL.CHEM. 278(2) 776-783 (2003).
 
3. FUJII, R., YOSHIDA, H., FUKUSUMI, S., HABATA, Y., HOSOYA, M., KAWAMATA, Y.,
YANO, T., HINUMA, S., KITADA, C., ASAMI, T., MORI, M., FUJISAWA, Y. AND
FUJINO, M.
Identification of a neuropeptide modified with bromine as an endogenous
ligand for GPR7.
J.BIOL.CHEM. 277(37) 34010-34016 (2002).

Documentation
Two closely related neuropeptide precursors, which share no significant
sequence similarity with other known neuropeptides, have recently been
identified and named preproneuropeptide B and preproneuropeptide W [1-3]. In
humans, each precursor contains a signal sequence and two dibasic cleavage
sites. Alternative cleavage of these sites results in long (29 or 30 amino
acid) and short (23 amino acid) forms of the resultant neuropeptides [1,2].
Murine, rat and bovine versions of preproneuropeptide B, however, contain
only the second cleavage site, resulting in only the long form of 
neuropeptide B [2,3].
 
Neuropeptide W has a more limited distribution in the brain than
neuropeptide B and is found at highest levels in the substantial nigra,
suggesting an involvement in locomotor control [2]. In the periphery,
neuropeptide W is more widespread than neuropeptide B. Like neuropeptide B,
it is expressed at high levels in testis, ovary, uterus, placenta, spleen,
lymph nodes and peripheral blood leukocytes, indicating potential roles in
the reproductive and immune systems [2]. In addition, however, neuropeptide
W has been found at high levels in the liver, stomach and trachea [2].
Intracerebroventricular administration of neuropeptide W in rats has been
reported to produce an acute increase in food intake and to stimulate
prolactin release [1].
 
PPNRPEPTIDEW is a 4-element fingerprint that provides a signature for the
neuropeptide W precursor. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved sections
spanning virtually the full alignment length, focusing on those areas
that characterise neuropeptide W precursors but distinguish them from
closely related neuropeptide B precursors - motif 1 lies in the central
region encoding neuropeptide W; and motifs 2-4 span the C-terminal region.
A single iteration on SPTR40_22f was required to reach convergence, no
further sequences being identified beyond the starting set.
Summary Information
2 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
42222
30000
20000
1234
True Positives
Q8K1M5        Q8MI35        
Sequence Titles
Q8K1M5      Prepro-neuropeptide W polypeptide - Rattus norvegicus (Rat). 
Q8MI35 Prepro-neuropeptide W polypeptide - Sus scrofa (Pig).
Scan History
SPTR40_22f 1  300  NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
HVASPRYHTVG Q8K1M5 45 45 -
HTASPRYHTVG Q8MI35 36 36 -

Motif 2 width=12
Element Seqn Id St Int Rpt
WRRALGGAAGPL Q8K1M5 71 15 -
WRRALRPAAGPL Q8MI35 62 15 -

Motif 3 width=15
Element Seqn Id St Int Rpt
LWEVRSRSSPAGLPV Q8K1M5 103 20 -
LWQVRRGSFRSGIPV Q8MI35 112 38 -

Motif 4 width=15
Element Seqn Id St Int Rpt
ATRSLRDLEGAGQPE Q8K1M5 119 1 -
APRSPRARGSEPQPE Q8MI35 128 1 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
HVASPRYHTVG Q8K1M5 45 45 -
HTASPRYHTVG Q8MI35 36 36 -

Motif 2 width=12
Element Seqn Id St Int Rpt
WRRALGGAAGPL Q8K1M5 71 15 -
WRRALRPAAGPL Q8MI35 62 15 -

Motif 3 width=15
Element Seqn Id St Int Rpt
LWEVRSRSSPAGLPV Q8K1M5 103 20 -
LWQVRRGSFRSGIPV Q8MI35 112 38 -

Motif 4 width=15
Element Seqn Id St Int Rpt
ATRSLRDLEGAGQPE Q8K1M5 119 1 -
APRSPRARGSEPQPE Q8MI35 128 1 -