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PR01888

Identifier
NROPEPTIDEBW  [View Relations]  [View Alignment]  
Accession
PR01888
No. of Motifs
3
Creation Date
23-FEB-2003
Title
Neuropeptide B/W family signature
Database References
PRINTS; PR01889 PPNRPEPTIDEB; PR01890 PPNRPEPTIDEW
Literature References
1. SHIMOMURA, Y., HARADA, M., GOTO, M., SUGO, T., MATSUMOTO, Y., ABE, M.,
WATANABE, T., ASAMI, T., KITADA, C., MORI, M., ONDA, H. AND FUJINO, M.
Identification of neuropeptide W as the endogenous ligand for orphan
G-protein-coupled receptors GPR7 and GPR8.
J.BIOL.CHEM. 277(39) 35826-35832 (2002).
 
2. BREZILLON, S., LANNOY, V., FRANSSEN, J.D., LE POUL, E., DUPRIEZ, V.,
LUCCHETTI, J., DETHEUX, M. AND PARMENTIER, M.
Identification of natural ligands for the orphan G protein-coupled receptors
GPR7 and GPR8.
J.BIOL.CHEM. 278(2) 776-783 (2003).
 
3. FUJII, R., YOSHIDA, H., FUKUSUMI, S., HABATA, Y., HOSOYA, M., KAWAMATA, Y.,
YANO, T., HINUMA, S., KITADA, C., ASAMI, T., MORI, M., FUJISAWA, Y. AND
FUJINO, M.
Identification of a neuropeptide modified with bromine as an endogenous
ligand for GPR7.
J.BIOL.CHEM. 277(37) 34010-34016 (2002).

Documentation
Two closely related neuropeptide precursors, which share no significant
sequence similarity with other known neuropeptides, have recently been
identified and named preproneuropeptide B and preproneuropeptide W [1-3]. In
humans, each precursor contains a signal sequence and two dibasic cleavage
sites. Alternative cleavage of these sites results in long (29 or 30 amino
acid) and short (23 amino acid) forms of the resultant neuropeptides [1,2].
Murine, rat and bovine versions of preproneuropeptide B, however, contain
only the second cleavage site, resulting in only the long form of 
neuropeptide B [2,3].
 
Neuropeptide B is expressed in both the central nervous system (CNS) and in
the periphery. In the CNS, highest levels of the peptide are found in the
substantia nigra and hypothalamus, suggesting a possible role in locomotor
control and the release of pituitary hormones [2]. In the periphery, the
peptide is most abundant in testis, ovary, uterus, placenta, spleen, lymph
nodes and peripheral blood leukocytes, indicating potential roles in the
reproductive and immune systems [2]. Unusually, neuropeptide B purified from
bovine hypothalamus was found to be brominated at its N-terminus [3].
 
Neuropeptide W has a more limited distribution in the brain than
neuropeptide B, and is found at highest levels in the substantial nigra,
again suggesting an involvement in locomotor control [2]. In the periphery,
neuropeptide W is more widespread than neuropeptide B. In addition to the
reproductive and immune tissues in which neuropeptide B is expressed,
neuropeptide W has been found at high levels in the liver, stomach and
trachea [2]. Intracerebroventricular administration of neuropeptide W in
rats has been reported to produce an acute increase in food intake and to
stimulate prolactin release [1].
 
NROPEPTIDEBW is a 3-element fingerprint that provides a signature for the
neuropeptide B/W family. The fingerprint was derived from an initial
alignment of 6 sequences: the motifs were drawn from the conserved N-
terminal portion of the alignment that encodes neuropeptides B and W.
A single iteration on SPTR40_22f was required to reach convergence, no
further sequences being identified beyond the starting set.
Summary Information
6 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3666
2000
123
True Positives
Q8K1M5        Q8K4P1        Q8K4P2        Q8MI35        
Q8MJV4 Q8NG41
Sequence Titles
Q8K1M5      Prepro-neuropeptide W polypeptide - Rattus norvegicus (Rat). 
Q8K4P1 Preproneuropeptide B - Mus musculus (Mouse).
Q8K4P2 Preproneuropeptide B - Rattus norvegicus (Rat).
Q8MI35 Prepro-neuropeptide W polypeptide - Sus scrofa (Pig).
Q8MJV4 Preproneuropeptide B - Bos taurus (Bovine).
Q8NG41 Preproneuropeptide B - Homo sapiens (Human).
Scan History
SPTR40_22f 1  150  NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
AWYKPTAGQGY Q8MJV4 24 24 -
AWYKPAAGHSS Q8NG41 24 24 -
AWYKPAAGPHH Q8K4P1 21 21 -
AWYKPAAGSHH Q8K4P2 21 21 -
AWYKHTASPRY Q8MI35 32 32 -
AWYKHVASPRY Q8K1M5 41 41 -

Motif 2 width=11
Element Seqn Id St Int Rpt
YYSVGRAAGLL Q8MJV4 34 -1 -
SYSVGRAAGLL Q8NG41 34 -1 -
HYSVGRASGLL Q8K4P1 31 -1 -
HYSVGRAAGLL Q8K4P2 31 -1 -
YHTVGRAAGLL Q8MI35 42 -1 -
YHTVGRASGLL Q8K1M5 51 -1 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SGFHRSPYARR Q8MJV4 45 0 -
SGLRRSPYARR Q8NG41 45 0 -
SSFHRFPSTRR Q8K4P1 42 0 -
SSFHRFPSTRR Q8K4P2 42 0 -
MGLRRSPYMWR Q8MI35 53 0 -
MGLRRSPYLWR Q8K1M5 62 0 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
AWYKPTAGQGY Q8MJV4 24 24 -
AWYKPAAGHSS Q8NG41 24 24 -
AWYKPAAGPHH Q8K4P1 21 21 -
AWYKPAAGSHH Q8K4P2 21 21 -
AWYKHTASPRY Q8MI35 32 32 -
AWYKHVASPRY Q8K1M5 41 41 -

Motif 2 width=11
Element Seqn Id St Int Rpt
YYSVGRAAGLL Q8MJV4 34 -1 -
SYSVGRAAGLL Q8NG41 34 -1 -
HYSVGRASGLL Q8K4P1 31 -1 -
HYSVGRAAGLL Q8K4P2 31 -1 -
YHTVGRAAGLL Q8MI35 42 -1 -
YHTVGRASGLL Q8K1M5 51 -1 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SGFHRSPYARR Q8MJV4 45 0 -
SGLRRSPYARR Q8NG41 45 0 -
SSFHRFPSTRR Q8K4P1 42 0 -
SSFHRFPSTRR Q8K4P2 42 0 -
MGLRRSPYMWR Q8MI35 53 0 -
MGLRRSPYLWR Q8K1M5 62 0 -