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PR01886

Identifier
PASS1  [View Relations]  [View Alignment]  
Accession
PR01886
No. of Motifs
3
Creation Date
14-FEB-2003
Title
Protein Associated with Small Stress protein 1 (PASS1) signature
Database References
Literature References
1. LIU, C., GILMONT, R.R., BENNDORF, R. AND WELSH, M.J.
Identification and characterization of a novel protein from Sertoli cells,
PASS1, that associates with mammalian small stress protein hsp27.
J.BIOL.CHEM 275(25) 18724-18731 (2000).

Documentation
The small heat shock proteins of vertebrates are thought to play a major
role in the cellular response to stress, and appear to play a role in a 
range of other physiological activities. Indeed, in response to many forms
of stress, including heat, the expression of heat shock proteins is 
increased, and coincidentally these cells become more stress-resistant. One
of these proteins is Heat Shock Protein (hsp) 27, for which 3 hypotheses 
currently exist to explain its mechanism of action: (i) it has chaperone-
like activity, serving as a site where denatured, unfolding proteins can 
bind until hsp70-dependent refolding can occur; (ii) it stabilises 
microfilaments, strengthening the cytoskeleton; and (iii) it enhances 
levels of the cellular antioxidant glutathione [1].
 
It was hypothesised that hsp27 associates with different protein partners
in order to effect its various cellular functions. Thus, a yeast two-hybrid 
screen was performed on a rat Sertoli cell cDNA library, which identified a 
novel binding protein, termed Protein Associated with Small Stress protein 
1 (PASS1). PASS1 is a 428-amino acid protein that, aside from its human 
orthologue, shares no significant similarity with known proteins, but does
show 34% identity to a genomic C.elegans sequence. In vitro experiments 
confirm the specific association of PASS1 and hsp27, while expression 
studies reveal PASS1 to be expressed in several tissues, with highest 
levels in the Sertoli cells of the testis. In addition, a smaller mRNA
than the original cDNA isolated from Sertoli cells was found in kidney, 
suggesting that alternative splicing may occur. Deletion experiments, 
resulting in truncated proteins, revealed the hsp27-association domain to
exist between amino acids 108 and 208 of PASS1. Finally, expression of 
PASS1 in cultured cell lines, which do not normally express PASS1, 
inhibited the ability of hsp27 to protect the cells against heat shock, 
confirming that PASS1 does indeed associate with hsp27 in vivo [1].
 
PASS1 is a 3-element fingerprint that provides a signature for the PASS1
protein family. The fingerprint was derived from an initial alignment of 3
sequences: the motifs were drawn from conserved regions spanning a short
region of the central portion of the alignment. Two iterations on 
SPTR40_20f were required to each convergence, at which point a true set 
comprising 4 sequences was identified.
Summary Information
4 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3444
2000
123
True Positives
Q8WWF0        Q96EW2        Q9H641        Q9R153        
Sequence Titles
Q8WWF0      HYPOTHETICAL 21.9 KDA PROTEIN - Homo sapiens (Human). 
Q96EW2 UNKNOWN (PROTEIN FOR MGC:20279) - Homo sapiens (Human).
Q9H641 CDNA: FLJ22623 FIS, CLONE HSI05687 - Homo sapiens (Human).
Q9R153 PROTEIN ASSOCIATING WITH SMALL STRESS PROTEIN PASS1 - Rattus norvegicus (Rat).
Scan History
SPTR40_20f 2  200  NSINGLE    
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
MLVCALKTAENPQNTR Q9H641 1 1 -
MLVCALKTAENPQNTR Q96EW2 290 290 -
MLVCTLKTAEDPHHPR Q9R153 290 290 -

Motif 2 width=13
Element Seqn Id St Int Rpt
WLNPTEVEETSHA Q9H641 18 1 -
WLNPTEVEETSHA Q96EW2 307 1 -
WLNPTEVEETSHE Q9R153 307 1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
NCCYLNAAVSAFFDRC Q9H641 32 1 -
NCCYLNAAVSAFFDRC Q96EW2 321 1 -
NSCYLNSAVCAFFDHC Q9R153 321 1 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
MLVCALKTAENPQNTR Q8WWF0 1 1 -
MLVCALKTAENPQNTR Q9H641 1 1 -
MLVCALKTAENPQNTR Q96EW2 290 290 -
MLVCTLKTAEDPHHPR Q9R153 290 290 -

Motif 2 width=13
Element Seqn Id St Int Rpt
WLNPTEVEETSHA Q8WWF0 18 1 -
WLNPTEVEETSHA Q9H641 18 1 -
WLNPTEVEETSHA Q96EW2 307 1 -
WLNPTEVEETSHE Q9R153 307 1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
NCCYLNAAVSAFFDRC Q8WWF0 32 1 -
NCCYLNAAVSAFFDRC Q9H641 32 1 -
NCCYLNAAVSAFFDRC Q96EW2 321 1 -
NSCYLNSAVCAFFDHC Q9R153 321 1 -