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PR01870

Identifier
CD2ANTIGEN  [View Relations]  [View Alignment]  
Accession
PR01870
No. of Motifs
5
Creation Date
27-JUL-2004
Title
T-cell surface CD2 antigen signature
Database References

PFAM; PF05790 CD2
INTERPRO; IPR008424
PDB; 1CDC; 1CDB; 1GYA; 1HNF
SCOP; 1CDC; 1CDB; 1GYA; 1HNF
CATH; 1CDC; 1CDB; 1GYA; 1HNF
Literature References
1. YANG, H. AND REINHERZ, E.L. 
Dynamic recruitment of human CD2 into lipid rafts. Linkage to
T cell signal transduction. 
J.BIOL.CHEM. 276 18775-18785 (2001).
 
2. BORCA, M.V., KUTISH, G.F., AFONSO, C.L., IRUSTA, P., CARRILLO, C.,
BRUN, A., SUSSMAN, M. AND ROCK, D.L. 
An African swine fever virus gene with similarity to the T-lymphocyte
surface antigen CD2 mediates hemadsorption. 
VIROLOGY 199 463-468 (1994).
 
3. WITHKA, J.M., WYSS, D.F., WAGNER, G., ARULANANDAM, A.R., 
REINHERZ, E.L. AND RECNY, M.A.
Structure of the glycosylated adhesion domain of human T lymphocyte
glycoprotein CD2.
STRUCTURE 1 69-81 (1993).
 
4. WYSS, D.F., CHOI, J.S., LI, J., KNOPPERS, M.H., WILLIS, K.J.,
ARULANANDAM, A.R., SMOLYAR, A., REINHERZ, E.L. AND WAGNER, G.
Conformation and function of the N-linked glycan in the adhesion domain
of human CD2. 
SCIENCE 269 1273-1278 (1995).

Documentation
CD2 is a type-I membrane protein that mediates adhesion between T
cells and other cell types via interaction through its ectodomain
with lymphocyte function-associated antigen (LFA-3)/CD58 and CD48/
BCM1. The protein is also implicated in the triggerring of T cells,
signal transduction being mediated via its long cytoplasmic tail [1].
CD2 belongs to a family of immunoglobulin (Ig)-like molecules, having
both C2-type and V-type Ig-like domains. In particular, it exhibits
structural and functional similarities to the African swine fever
virus (ASFV) protein, LMW8-DR, a protein involved in cell-cell
adhesion and immune response modulation, suggesting a possible role
in the pathogenesis of ASFV infection [2].
 
The adhesion domain of human CD2 requires N-linked carbohydrate to
maintain its native conformation and ability to bind CD58 [3]. By 
contrast, rat CD2 does not require N-linked carbohydrate, and binds
to a different ligand, CD48 [3]. The 3D structure of the glycosylated 
human form of this domain has been determined by NMR spectroscopy [3].
Overall, the structure resembles the typical beta-barrel of an Ig 
variable domain. Mutagenesis of all residues in the vicinity of the
glycan suggests that the glycan is not a component of the CD2-CD58
interface, but rather, stabilises the protein fold by counterbalancing
an unfavorable clustering of 5 positive charges centred about Lys-61
of CD2 [4].
 
CD2ANTIGEN is a 5-element fingerprint that provides a signature for 
T-cell surface CD2 antigens. The fingerprint was derived from an
initial alignment of 6 sequences: the motifs were drawn from the
N-terminal half of the alignment, focusing on those regions that
characterise CD2 antigen but distinguish them from closely-
related members of the Ig-like CD2 family - the motifs span the
V-type Ig-like extracellular domain. Two iterations on SPTR41_24f
were required to reach convergence, at which point a true set
comprising 7 sequences was identified.
Summary Information
7 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
577777
400000
300000
200000
12345
True Positives
CD2_HORSE     CD2_HUMAN     CD2_MOUSE     CD2_RAT       
Q8WNV8 Q96TE5 Q9R201
Sequence Titles
CD2_HORSE   T-cell surface antigen CD2 precursor - Equus caballus (Horse). 
CD2_HUMAN T-cell surface antigen CD2 precursor (T-cell surface antigen T11/Leu-5) (LFA-2) (LFA-3 receptor) (Erythrocyte receptor) (Rosette receptor) - Homo sapiens (Human).
CD2_MOUSE T-cell surface antigen CD2 precursor (T-cell surface antigen T11/Leu-5) (LFA-2) (LFA-3 receptor) - Mus musculus (Mouse).
CD2_RAT T-cell surface antigen CD2 precursor (T-cell surface antigen T11/Leu-5) (LFA-2) (LFA-3 receptor) (OX-34 antigen) - Rattus norvegicus (Rat).
Q8WNV8 T-lymphocyte surface CD2 antigen - Felis silvestris catus (Cat).
Q96TE5 DJ655N15.1 (CD2 antigen (p50), sheep red blood cell receptor) - Homo sapiens (Human).
Q9R201 T-cell surface antigen CD2 - Mus musculus (Mouse).
Scan History
SPTR41_24f 2  200  NSINGLE    
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
WGALGQDINLDIP CD2_HUMAN 34 34 -
WGVLGHGITLNIP CD2_MOUSE 29 29 -
WGVLGHGITLNIP Q9R201 29 29 -
WGALGHGINLNIP CD2_RAT 29 29 -
WGTLGQDINLDIP Q8WNV8 28 28 -
LGALERDINLDIP CD2_HORSE 31 31 -

Motif 2 width=14
Element Seqn Id St Int Rpt
FQMSDDIDDIKWEK CD2_HUMAN 48 1 -
FQMTDDIDEVRWVR CD2_MOUSE 43 1 -
FQMTDDIDEVRWVR Q9R201 43 1 -
FQMTDDIDEVRWER CD2_RAT 43 1 -
DSQGINIDDIHWEK Q8WNV8 41 0 -
FQMSEHVEDIQWSK CD2_HORSE 45 1 -

Motif 3 width=13
Element Seqn Id St Int Rpt
YKLFKNGTLKIKH CD2_HUMAN 84 22 -
YEVLANGSLKIKK CD2_MOUSE 77 20 -
YEVLANGSLKIKK Q9R201 77 20 -
FEILANGDLKIKN CD2_RAT 77 20 -
YNVSMNGTLKIKH Q8WNV8 75 20 -
YEVLKNGTLKIKH CD2_HORSE 79 20 -

Motif 4 width=18
Element Seqn Id St Int Rpt
LKTDDQDIYKVSIYDTKG CD2_HUMAN 97 0 -
MMRNDSGTYNVMVYGTNG CD2_MOUSE 91 1 -
MMRNDSGTYNVMVYGTNG Q9R201 91 1 -
LTRDDSGTYNVTVYSTNG CD2_RAT 90 0 -
LMLEDCDTYKVVIYDKDG Q8WNV8 88 0 -
LERIHEGTYKVDAYDSDG CD2_HORSE 92 0 -

Motif 5 width=15
Element Seqn Id St Int Rpt
GKNVLEKIFDLKIQE CD2_HUMAN 114 -1 -
GMTRLEKDLDVRILE CD2_MOUSE 108 -1 -
GMTRLEKDLDVRILE Q9R201 108 -1 -
GTRILDKALDLRILE CD2_RAT 107 -1 -
GKNVLDKTFQLKIQE Q8WNV8 105 -1 -
GKNVLEETFHLSLLE CD2_HORSE 109 -1 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
WGALGQDINLDIP CD2_HUMAN 34 34 -
WGALGQDINLDIP Q96TE5 34 34 -
WGVLGHGITLNIP CD2_MOUSE 29 29 -
WGVLGHGITLNIP Q9R201 29 29 -
WGALGHGINLNIP CD2_RAT 29 29 -
WGTLGQDINLDIP Q8WNV8 28 28 -
LGALERDINLDIP CD2_HORSE 31 31 -

Motif 2 width=14
Element Seqn Id St Int Rpt
FQMSDDIDDIKWEK CD2_HUMAN 48 1 -
FQMSDDIDDIKWEK Q96TE5 48 1 -
FQMTDDIDEVRWVR CD2_MOUSE 43 1 -
FQMTDDIDEVRWVR Q9R201 43 1 -
FQMTDDIDEVRWER CD2_RAT 43 1 -
DSQGINIDDIHWEK Q8WNV8 41 0 -
FQMSEHVEDIQWSK CD2_HORSE 45 1 -

Motif 3 width=13
Element Seqn Id St Int Rpt
YKLFKNGTLKIKH CD2_HUMAN 84 22 -
YKLFKNGTLKIKH Q96TE5 84 22 -
YEVLANGSLKIKK CD2_MOUSE 77 20 -
YEVLANGSLKIKK Q9R201 77 20 -
FEILANGDLKIKN CD2_RAT 77 20 -
YNVSMNGTLKIKH Q8WNV8 75 20 -
YEVLKNGTLKIKH CD2_HORSE 79 20 -

Motif 4 width=18
Element Seqn Id St Int Rpt
LKTDDQDIYKVSIYDTKG CD2_HUMAN 97 0 -
LKTDDQDIYKVSIYDTKG Q96TE5 97 0 -
MMRNDSGTYNVMVYGTNG CD2_MOUSE 91 1 -
MMRNDSGTYNVMVYGTNG Q9R201 91 1 -
LTRDDSGTYNVTVYSTNG CD2_RAT 90 0 -
LMLEDCDTYKVVIYDKDG Q8WNV8 88 0 -
LERIHEGTYKVDAYDSDG CD2_HORSE 92 0 -

Motif 5 width=15
Element Seqn Id St Int Rpt
GKNVLEKIFDLKIQE CD2_HUMAN 114 -1 -
GKNVLEKIFDLKIQE Q96TE5 114 -1 -
GMTRLEKDLDVRILE CD2_MOUSE 108 -1 -
GMTRLEKDLDVRILE Q9R201 108 -1 -
GTRILDKALDLRILE CD2_RAT 107 -1 -
GKNVLDKTFQLKIQE Q8WNV8 105 -1 -
GKNVLEETFHLSLLE CD2_HORSE 109 -1 -