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PR01858

Identifier
ADAMTS1  [View Relations]  [View Alignment]  
Accession
PR01858
No. of Motifs
7
Creation Date
13-MAR-2003
Title
ADAM-TS1 protein signature
Database References
PRINTS; PR01857 ADAMTSFAMILY
Literature References
1. WERB, Z.
ECM and cell surface proteolysis: regulating cellular ecology.
CELL 91 439-442 (1997).
 
2. KUNO, K., KANADA, N., NAKASHIMA, E., FUJIKI, F., ICHIMURA, F. AND 
MATSUSHIMA, K.
Molecular cloning of a gene encoding a new type of metalloproteinase-
disintegrin family protein with thrombospondin motifs as an inflammation 
associated gene.
J.BIOL.CHEM. 272 556-562 (1997).
 
3. HURSKAINEN, T., HIROHATA, S., SELDIN, M. AND APTE, S. 
ADAM-TS5, ADAM-TS6 and ADAM-TS7, novel members of a new family of zinc 
metalloproteases (ADAM-TS, A disintegrin and metalloprotease domain with 
thrombospondin type I motifs). General features and genomic distribution of
the ADAM-TS family. 
J.BIOL.CHEM. 274 25555-25563 (1999).
 
4. COLIGE, A., SIERON, A., LI, S., SCHWARZE, U., PETTY, E., WERTELECKI, W.,
WILCOX, W., KRAKOV, D., COHN, D., REARDON, W., BYERS, P., LAPIERE, C., 
PROCKOP,, D. AND NUSGENS, B.
Human Ehlers-Danlos syndrome type VII C and bovine dermatosparaxis are 
caused by mutations in the procollagen I N-proteinase gene. 
AM.J.HUM.GENET. 65 308-317 (1999).
 
5. TORTORELLA, M., BURN, T., PRATTA, M., ABBASZADE, I., HOLLIS, J.,  LIU, R.,
ROSENFELD, S., COPELAND, R., DECICCO, C., WYNN, R.,  ROCKWELL, A., YANG, F.,
DUKE, J., SOLOMON, K., GEORGE, H., BRUCKNER, R., NAGASE, H., ITOH, Y., 
ELLIS, D., ROSS, H., WISWALL, B., MURPHY, K.,  HILLMAN, M., HOLLIS, G., 
NEWTON, R., MAGOLDA, R., TRZASKOS, J. AND ARNER, E.
Purification and cloning of aggrecanase-1: a member of the ADAMTS family of
proteins. 
SCIENCE 284 1664-1666 (1999).

Documentation
Proteolysis of the extracellular matrix plays a critical role in
establishing tissue architecture during development, and in tissue 
degradation in diseases such as cancer, arthritis, Alzheimer's disease
and a variety of inflammatory conditions [1]. The proteolytic enzymes 
responsible for this process are members of diverse protease families, 
including the secreted zinc metalloproteases (MPs) [1]. Recently, a new
MP family, ADAM-TS (a disintegrin-like and metalloprotease domain with 
thrombospondin type I modules) has been identified. The family consists
of at least 20 members that share a high degree of sequence similarity
and conserved domain organisation [2,3].
 
The defining domains of the ADAM-TS family are (from N- to C-termini) a
pre-pro metalloprotease domain of the reprolysin type, a snake venom
disintegrin-like domain, a thrombospondin type-I (TS) module, a cysteine-
rich region, and a cysteine-free (spacer) domain [3]. Domain organisation
following the spacer domain C-terminus shows some variability in certain
ADAM-TS members, principally in the number of additional TS domains.
 
Members of the ADAM-TS family have been implicated in a range of diseases. 
ADAM-TS1, for example, is reported to be involved in inflammation and cancer
cachexia [2], whilst recessively inherited ADAM-TS2 mutations cause
Ehlers-Danlos syndrome type VIIC, a disorder characterised clinically by 
severe skin fragility [4]. ADAM-TS4 is an aggrecanase involved in arthritic
destruction of cartilage [5].
 
ADAM-TS1 was originally cloned in mice [2]. Human and rat orthologues have 
also been identified. Expression of ADAMTS-1 is closely associated with
acute inflammation [2].
 
ADAMTS1 is a 7-element fingerprint that provides a signature for the
ADAM-TS1 proteins. The fingerprint was derived from an initial alignment of
3 sequences: the motifs were drawn from conserved regions spanning virtually
the full alignment length, focusing on those sections that characterise 
ADAM-TS1 proteins and distinguish them from other family members - motifs 
1-4 reside in the N-terminal region preceding the metalloprotease domain; 
motif 5 lies in the metalloprotease domain; motif 6 lies in the cysteine-
rich region; and motif 7 resides in the the third TS module. Two iterations 
on SPTR40_22f were required to reach convergence, at which point a true set 
comprising 4 sequences was identified.
Summary Information
4 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
74444444
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
ATS1_HUMAN    ATS1_MOUSE    ATS1_RAT      Q8NE26        
Sequence Titles
ATS1_HUMAN  ADAMTS-1 precursor (EC 3.4.24.-) (A disintegrin and metalloproteinase with thrombospondin motifs 1) (ADAM-TS 1) (ADAM-TS1) (METH-1) - Homo sapiens (Human). 
ATS1_MOUSE ADAMTS-1 precursor (EC 3.4.24.-) (A disintegrin and metalloproteinase with thrombospondin motifs 1) (ADAM-TS 1) (ADAM-TS1) - Mus musculus (Mouse).
ATS1_RAT ADAMTS-1 precursor (EC 3.4.24.-) (A disintegrin and metalloproteinase with thrombospondin motifs 1) (ADAM-TS 1) (ADAM-TS1) - Rattus norvegicus (Rat).
Q8NE26 Hypothetical protein - Homo sapiens (Human).
Scan History
SPTR40_22f 2  150  NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
MQRAVPEGFGRRKLGSDMG ATS1_HUMAN 1 1 -
MQPKVPLGSRKQKPCSDMG ATS1_MOUSE 1 1 -
MQPEVPLGSGKLKPCSDMG ATS1_RAT 1 1 -

Motif 2 width=12
Element Seqn Id St Int Rpt
LQNVGRKSGSET ATS1_HUMAN 102 82 -
LQTVGRSPGSEA ATS1_MOUSE 107 87 -
LQTVGRSPGSEA ATS1_RAT 106 86 -

Motif 3 width=13
Element Seqn Id St Int Rpt
ASERLATAAPGEK ATS1_HUMAN 164 50 -
ATERLAPAVPEEE ATS1_MOUSE 172 53 -
ATERLVPAEPKEE ATS1_RAT 171 53 -

Motif 4 width=15
Element Seqn Id St Int Rpt
VGGTCGVVDDEPRPT ATS1_HUMAN 194 17 -
GGAKCGVMDDETLPT ATS1_MOUSE 202 17 -
GGAKCGVMDEETLPT ATS1_RAT 201 17 -

Motif 5 width=12
Element Seqn Id St Int Rpt
HDEQKGPEVTSN ATS1_HUMAN 313 104 -
YEEQKGPEVTSN ATS1_MOUSE 314 97 -
YEEQKGPEVTSN ATS1_RAT 313 97 -

Motif 6 width=10
Element Seqn Id St Int Rpt
PDNNGKTFRE ATS1_HUMAN 614 289 -
PDNNGKTFRE ATS1_MOUSE 615 289 -
PDNNGKTFRE ATS1_RAT 614 289 -

Motif 7 width=17
Element Seqn Id St Int Rpt
GYKKRSLKCLSHDGGVL ATS1_HUMAN 927 303 -
GYKKRTLKCVSHDGGVL ATS1_MOUSE 928 303 -
GYKKRTLKCLSHDGGVL ATS1_RAT 927 303 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
MQRAVPEGFGRRKLGSDMG ATS1_HUMAN 1 1 -
MQRAVPEGFGRRKLGSDMG Q8NE26 1 1 -
MQPKVPLGSRKQKPCSDMG ATS1_MOUSE 1 1 -
MQPEVPLGSGKLKPCSDMG ATS1_RAT 1 1 -

Motif 2 width=12
Element Seqn Id St Int Rpt
LQNVGRKSGSET ATS1_HUMAN 102 82 -
LQNVGRKSGSET Q8NE26 102 82 -
LQTVGRSPGSEA ATS1_MOUSE 107 87 -
LQTVGRSPGSEA ATS1_RAT 106 86 -

Motif 3 width=13
Element Seqn Id St Int Rpt
ASERLATAAPGEK ATS1_HUMAN 164 50 -
ASERLATAAPGEK Q8NE26 164 50 -
ATERLAPAVPEEE ATS1_MOUSE 172 53 -
ATERLVPAEPKEE ATS1_RAT 171 53 -

Motif 4 width=15
Element Seqn Id St Int Rpt
VGGTCGVVDDEPRPT ATS1_HUMAN 194 17 -
VGGTCGVVDDEPRPT Q8NE26 194 17 -
GGAKCGVMDDETLPT ATS1_MOUSE 202 17 -
GGAKCGVMDEETLPT ATS1_RAT 201 17 -

Motif 5 width=12
Element Seqn Id St Int Rpt
HDEQKGPEVTSN ATS1_HUMAN 313 104 -
HDEQKGPEVTSN Q8NE26 313 104 -
YEEQKGPEVTSN ATS1_MOUSE 314 97 -
YEEQKGPEVTSN ATS1_RAT 313 97 -

Motif 6 width=10
Element Seqn Id St Int Rpt
PDNNGKTFRE ATS1_HUMAN 614 289 -
PDNNGKTFRE Q8NE26 614 289 -
PDNNGKTFRE ATS1_MOUSE 615 289 -
PDNNGKTFRE ATS1_RAT 614 289 -

Motif 7 width=17
Element Seqn Id St Int Rpt
GYKKRSLKCLSHDGGVL ATS1_HUMAN 927 303 -
GYKKRSLKCLSHDGGVL Q8NE26 927 303 -
GYKKRTLKCVSHDGGVL ATS1_MOUSE 928 303 -
GYKKRTLKCLSHDGGVL ATS1_RAT 927 303 -