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PR01853

Identifier
YAJCTRNLCASE  [View Relations]  [View Alignment]  
Accession
PR01853
No. of Motifs
3
Creation Date
04-FEB-2003  (UPDATE 05-JUN-2003)
Title
Bacterial YajC preprotein translocase signature
Database References

BLOCKS; IPB003849
PFAM; PF02699 DUF219
INTERPRO; IPR003849
Literature References
1. BIEKER, K.L., PHILLIPS, G.J. AND SILHAVY, T.J.
The sec and prl genes of Escherichia coli.
J.BIOENERG.BIOMEMBR. 22 291-310 (1990).
 
2. DUONG, F. AND WICKNER, W.
The SecDFyajC domain of preprotein translocase controls preprotein movement 
by regulating SecA membrane cycling.
EMBO J. 16 4871-4879 (1997).
 
3. VEMULAPALLI, R., DUNCAN, J., BOYLE, S.M., SPIRANGANATHAN, N., TOTH, T.E.
AND SCHURIG, G.G.
Cloning and sequencing of yaC and secD homologs of Brucella abortus and 
demonstration of immune responses to YajC in mice vaccinated with B. abortus 
RB51.
INFECT.IMMUN. 66 5684-5691 (1998).
 
4. NOUWEN, N. AND DRIESSEN, A.J.
SecDFyajC forms a heterotetrameric complex with YidC.
MOL.MICROBIOL. 44 1397-1405 (2002).

Documentation
Secretion across the inner membrane in some Gram-negative bacteria occurs 
via the preprotein translocase pathway. Proteins are produced in the 
cytoplasm as precursors, and require a chaperone subunit to direct them to 
the translocase component [1]. From there, the mature proteins are either
targeted to the outer membrane, or remain as periplasmic proteins [1]. The 
translocase protein subunits are encoded on the bacterial chromosome. 
 
The translocase itself comprises 7 proteins, including a chaperone (SecB), 
ATPase (SecA), an integral membrane complex (SecY, SecE and SecG), and two 
additional membrane proteins that promote the release of the mature peptide 
into the periplasm (SecD and SecF) [1]. Other cytoplasmic/periplasmic 
proteins play a part in preprotein translocase activity, namely YidC and 
YajC [2]. The latter is bound in a complex to SecD and SecF, and plays a
part in stabilising and regulating secretion through the SecYEG integral
membrane component via SecA [2]. 
 
Homologues of the YajC gene have been found in a range of pathogenic and 
commensal microbes. Brucella abortis YajC- and SecD-like proteins were shown
to stimulate a Th1 cell-mediated immune response in mice, and conferred 
protection when challenged with B.abortis [3]. Therefore, these proteins may
have an antigenic role as well as a secretory one in virulent bacteria [3].
A number of previously uncharacterised "hypothetical" proteins also show
similarity to E.coli YajC, suggesting that this family is wider than first 
thought [3].
 
More recently, the precise interactions between the E.coli SecYEG complex, 
SecD, SecF, YajC and YidC have been studied [4]. Rather than acting 
individually, the four proteins form a heterotetrameric complex and 
associate with the SecYEG heterotrimeric complex [4]. The SecF and YajC 
subunits link the complex to the integral membrane translocase.
 
YAJCTRNLCASE is a 3-element fingerprint that provides a signature for the
bacterial YajC preprotein translocases. The fingerprint was derived from an
initial alignment of 12 sequences: the motifs were drawn from conserved 
regions spanning virtually the full alignment length. Five iterations on
SPTR40_20f were required to reach convergence, at which point a true set
comprising 39 sequences was identified. Six partial matches were also
found, all of which appear to be related proteins that failed to make a 
significant match with motif 1.
Summary Information
  39 codes involving  3 elements
6 codes involving 2 elements
Composite Feature Index
3393939
2066
123
True Positives
Q47777        Q8XFR4        Q8XJ17        Q8XVW3        
Q8Y701 Q8ZC34 Q92BI5 Q92H78
Q92Q20 Q98LD5 Q99TL5 Q9A6U1
Q9AE08 Q9CP36 Q9HXI0 Q9JP43
Q9JVJ6 Q9K0J1 Q9KJW7 Q9KTY8
Q9PGS4 Q9PNK0 Y117_CHLMU Y134_BUCAI
Y585_RICPR Y651_BORBU Y741_CHLTR Y859_THEMA
Y884_CHLPN YAJC_BRUAB YAJC_ECOLI YAJC_HAEIN
YC29_BACHD YC54_AQUAE YF51_HELPJ YF51_HELPY
YP88_MYCLE YP88_MYCTU YRBF_BACSU
True Positive Partials
Codes involving 2 elements
Q97GT4 Q97NJ7 Q97SK6 Q99XY0
Q9CDT0 Y40A_TREPA
Sequence Titles
Q47777      ORF11 - Enterococcus faecalis (Streptococcus faecalis). 
Q8XFR4 PREPROTEIN TRANSLOCASE IISP FAMILY, MEMBRANE SUBUNIT (PUTATIVE MEMBRANE PROTEIN) - Salmonella typhimurium, and Salmonella typhi.
Q8XJ17 HYPOTHETICAL PROTEIN CPE1944 - Clostridium perfringens.
Q8XVW3 PROBABLE TRANSMEMBRANE PROTEIN - Ralstonia solanacearum (Pseudomonas solanacearum).
Q8Y701 HYPOTHETICAL PROTEIN LMO1529 - Listeria monocytogenes.
Q8ZC34 PUTATIVE MEMBRANE PROTEIN - Yersinia pestis.
Q92BI5 HYPOTHETICAL PROTEIN LIN1564 - Listeria innocua.
Q92H78 HYPOTHETICAL PROTEIN RC0893 - Rickettsia conorii.
Q92Q20 PROBABLE YAJC PROTEIN - Rhizobium meliloti (Sinorhizobium meliloti).
Q98LD5 PROBABLE MEMBRANE PROTEIN - Rhizobium loti (Mesorhizobium loti).
Q99TL5 HYPOTHETICAL PROTEIN SAV1638 - Staphylococcus aureus (strain Mu50 / ATCC 700699), and Staphylococcus aureus (strain N315).
Q9A6U1 PREPROTEIN TRANSLOCASE, YAJC SUBUNIT - Caulobacter crescentus.
Q9AE08 HYPOTHETICAL 13.5 KDA PROTEIN - Corynebacterium glutamicum (Brevibacterium flavum).
Q9CP36 YAJC - Pasteurella multocida.
Q9HXI0 HYPOTHETICAL PROTEIN PA3822 - Pseudomonas aeruginosa.
Q9JP43 PUTATIVE MEMBRANE PROTEIN - Rhizobium meliloti (Sinorhizobium meliloti).
Q9JVJ6 HYPOTHETICAL PROTEIN NMA0811 - Neisseria meningitidis (serogroup A).
Q9K0J1 HYPOTHETICAL PROTEIN NMB0606 - Neisseria meningitidis (serogroup B).
Q9KJW7 YAJC HOMOLOG - Bartonella bacilliformis.
Q9KTY8 HYPOTHETICAL PROTEIN VC0742 - Vibrio cholerae.
Q9PGS4 PREPROTEIN TRANSLOCASE YAJC SUBUNIT - Xylella fastidiosa.
Q9PNK0 PUTATIVE MEMBRANE PROTEIN - Campylobacter jejuni.
Y117_CHLMU Hypothetical protein TC0117 - Chlamydia muridarum.
Y134_BUCAI Hypothetical protein BU134 - Buchnera aphidicola (subsp. Acyrthosiphon pisum) (Acyrthosiphon pisum symbiotic bacterium).
Y585_RICPR Hypothetical protein RP585 - Rickettsia prowazekii.
Y651_BORBU Hypothetical protein BB0651 - Borrelia burgdorferi (Lyme disease spirochete).
Y741_CHLTR Hypothetical protein CT741 - Chlamydia trachomatis.
Y859_THEMA Hypothetical protein TM0859 - Thermotoga maritima.
Y884_CHLPN Hypothetical protein CPn0884/CP0982/CPj0884 - Chlamydia pneumoniae (Chlamydophila pneumoniae).
YAJC_BRUAB Immunogenic membrane protein YAJC - Brucella abortus.
YAJC_ECOLI Hypothetical protein yajC - Escherichia coli, Escherichia coli O157:H7, and Shigella flexneri.
YAJC_HAEIN Hypothetical protein HI0241 - Haemophilus influenzae.
YC29_BACHD Hypothetical protein BH1229 - Bacillus halodurans.
YC54_AQUAE Hypothetical protein AQ_1254 - Aquifex aeolicus.
YF51_HELPJ Hypothetical protein JHP1448 - Helicobacter pylori J99 (Campylobacter pylori J99).
YF51_HELPY Hypothetical protein HP1551 - Helicobacter pylori (Campylobacter pylori).
YP88_MYCLE Hypothetical 12.8 kDa protein ML0486 precursor - Mycobacterium leprae.
YP88_MYCTU Hypothetical 13.0 kDa protein Rv2588c precursor - Mycobacterium tuberculosis.
YRBF_BACSU Hypothetical 9.9 kDa protein in SPOVB-TGT intergenic region - Bacillus subtilis.

Q97GT4 UNCHARACTERIZED SECRETED PROTEIN, YAJC FAMILY - Clostridium acetobutylicum.
Q97NJ7 PREPROTEIN TRANSLOCASE, YAJC SUBUNIT - Streptococcus pneumoniae.
Q97SK6 PREPROTEIN TRANSLOCASE, YAJC SUBUNIT - Streptococcus pneumoniae.
Q99XY0 HYPOTHETICAL PROTEIN SPY1968 - Streptococcus pyogenes.
Q9CDT0 UNKNOWN PROTEIN - Lactococcus lactis (subsp. lactis) (Streptococcus lactis).
Y40A_TREPA Hypothetical protein TP0409.1 - Treponema pallidum.
Scan History
SPTR40_20f 5  700  NSINGLE    
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
MSLILMLVVFGLIFYFMILRP YAJC_ECOLI 21 21 -
TSMLIMLVVFGLIFYFMILRP Q8ZC34 22 22 -
LMSILPFILIFVIMYFLIIRP YAJC_BRUAB 20 20 -
IVTFIPIILMIVLFYFLLIRP Q92BI5 5 5 -
LTSLLPLVVLFAIFYFLVIRP Q9PNK0 7 7 -
LMSILPFLLIFVVMYFLIIRP Q92Q20 21 21 -
MSTLFIFVIFGLIFYFMIYRP YAJC_HAEIN 7 7 -
MSMLFIFVIFGLIFYFMIYRP Q9CP36 9 9 -
LQMFFLPVILIAVMYFSIIRP Q9PGS4 31 31 -
LRSLLVFVPVIAIFWFLVISP Y651_BORBU 21 21 -
DVVRYLYSFYFVIMYFLIIRP Q9KJW7 15 15 -
LMNILPIILMVVLFYFMLIRP Q9A6U1 10 10 -

Motif 2 width=19
Element Seqn Id St Int Rpt
PQQKRTKEHKKLMDSIAKG YAJC_ECOLI 41 -1 -
PQQKRAKEHKKLMDSIGKG Q8ZC34 42 -1 -
PQRTQMKKRQEMLNSVRRG YAJC_BRUAB 40 -1 -
PQQKRQKEVQNMQSSLAKG Q92BI5 25 -1 -
PQQKQAKAHKQMLESLQKG Q9PNK0 27 -1 -
PQRAQMKRREELLKNIRRG Q92Q20 41 -1 -
PQAKRNKEHKKLMSELAKG YAJC_HAEIN 27 -1 -
PQAKRNKEHKKLMSELAKG Q9CP36 29 -1 -
PQMKRQKEYKSMMDHLGCG Q9PGS4 51 -1 -
PQRKEEKNKKEMIKNLKKG Y651_BORBU 41 -1 -
PQRVQMKKRQEMLNAVRRG Q9KJW7 35 -1 -
PQQKRQKEHLNMLNNLKRN Q9A6U1 30 -1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
GDEVLTNGGLVGRVTKVAE YAJC_ECOLI 59 -1 -
GDEVLTSGGFIGRVTKVAE Q8ZC34 60 -1 -
GDTVVTGGGIVGKVLKVVD YAJC_BRUAB 58 -1 -
GDKIITIGGLHGIVEAIED Q92BI5 43 -1 -
GDKIITNGGLICEVVKPEE Q9PNK0 45 -1 -
GDQVVTGGGIVGKVTKVVD Q92Q20 59 -1 -
GTEVLTAGGVIGKITKVTE YAJC_HAEIN 45 -1 -
GTEVLIAGGLIGRITKVSP Q9CP36 47 -1 -
GDEVITAGGIAGVVSVIGD Q9PGS4 69 -1 -
GDKVLTIGGIFGVVKKLGD Y651_BORBU 59 -1 -
GDVIVTGGGIIGKVIKVHD Q9KJW7 53 -1 -
NDTVVLSSGMIGKIVRVED Q9A6U1 48 -1 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
MSLILMLVVFGLIFYFMILRP Q8XFR4 21 21 -
MSLILMLVVFGLIFYFMILRP YAJC_ECOLI 21 21 -
TSMLIMLVVFGLIFYFMILRP Q8ZC34 22 22 -
LMSILPFILIFVIMYFLIIRP YAJC_BRUAB 20 20 -
LMSFLPIILMFVVLWFIMIRP Q8XVW3 21 21 -
IVTFIPIILMIVLFYFLLIRP Q8Y701 4 4 -
IVTFIPIILMIVLFYFLLIRP Q92BI5 5 5 -
LTSLLPLVVLFAIFYFLVIRP Q9PNK0 7 7 -
LMSILPFLLIFVVMYFLIIRP Q92Q20 21 21 -
LMSILPFLLIFVEMYFLIIRP Q9JP43 21 21 -
MSTLFIFVIFGLIFYFMIYRP YAJC_HAEIN 7 7 -
GGFLIPLILMFAIFYFLLIRP YC29_BACHD 5 5 -
GFEWVFLVGFLVIFYLMIWRP Q9HXI0 24 24 -
FISILPFVLIFVIMYFLIIRP Q98LD5 19 19 -
LGTLVPIILMFAVLYFLLIRP YRBF_BACSU 6 6 -
LTTLLPLVVLFLIFYFLIVRP YF51_HELPY 8 8 -
FEMIIMLAMFAVIFYFMIYRP Q9KTY8 20 20 -
LTSLIPMVLIFAVFYFLLLRP Q92H78 35 35 -
MSMLFIFVIFGLIFYFMIYRP Q9CP36 9 9 -
VAQFAPLVLIMVVFYFLIMRP Q9JVJ6 5 5 -
VAQFAPLVLIMVVFYFLIMRP Q9K0J1 5 5 -
LTSLIPMILIFAVFYFLLLRP Y585_RICPR 35 35 -
ITTLLPLLVLFLIFYFLIVRP YF51_HELPJ 8 8 -
LFQIIFLVAIFLMFYFLIIRP YC54_AQUAE 11 11 -
LQMFFLPVILIAVMYFSIIRP Q9PGS4 31 31 -
YSLIFMAVIFLLIFYFMLFRP Y134_BUCAI 22 22 -
LRSLLVFVPVIAIFWFLVISP Y651_BORBU 21 21 -
FSLLIYIVVIFAVMYFLMIRP Q99TL5 3 3 -
DVVRYLYSFYFVIMYFLIIRP Q9KJW7 15 15 -
LMNILPIILMVVLFYFMLIRP Q9A6U1 10 10 -
GSLLFMLIFFIAIFYFMIILP Y859_THEMA 26 26 -
ATMVLPFILMFGVFYFLLILP Q8XJ17 7 7 -
VQPAVMLAIAILFFYFILWRP Y884_CHLPN 32 32 -
MKLMLIMLLVIVAMYFYLFRT Q47777 1 1 -
GQPAVMLGIAIVFFYFILWRP Y117_CHLMU 32 32 -
GQPAVMLGIAIVFFYFILWRP Y741_CHLTR 32 32 -
LVLLLLFLLIMGGFMFFASRR YP88_MYCLE 4 4 -
IVFLIILLAIFIVPTFLMSRR Q9AE08 3 3 -
FVLFLPFLLIMGGFMYFASRR YP88_MYCTU 4 4 -

Motif 2 width=19
Element Seqn Id St Int Rpt
PQQKRTKEHKKLMDSIAKG Q8XFR4 41 -1 -
PQQKRTKEHKKLMDSIAKG YAJC_ECOLI 41 -1 -
PQQKRAKEHKKLMDSIGKG Q8ZC34 42 -1 -
PQRTQMKKRQEMLNSVRRG YAJC_BRUAB 40 -1 -
PQMKRQKETKTMLEALAKG Q8XVW3 41 -1 -
PQQKRQKEVQNMQSSLAKG Q8Y701 24 -1 -
PQQKRQKEVQNMQSSLAKG Q92BI5 25 -1 -
PQQKQAKAHKQMLESLQKG Q9PNK0 27 -1 -
PQRAQMKRREELLKNIRRG Q92Q20 41 -1 -
PQRAQMKRREELLKNIRRG Q9JP43 41 -1 -
PQAKRNKEHKKLMSELAKG YAJC_HAEIN 27 -1 -
PQQKRQKQIQEMHNNLQRG YC29_BACHD 25 -1 -
PQAKRAKEHKNLLGNLQKG Q9HXI0 44 -1 -
PQRTQLKKRGEMLAAVRRG Q98LD5 39 -1 -
PQQKQQKAVRQMQEELKKG YRBF_BACSU 26 -1 -
PQRQQQKKHKEMIESLTKG YF51_HELPY 28 -1 -
PQAKRVKEHKNLMAAMAKG Q9KTY8 40 -1 -
PQETRRKEREKLVSEVKKG Q92H78 55 -1 -
PQAKRNKEHKKLMSELAKG Q9CP36 29 -1 -
PQQKKFKAHQAMLAALKVG Q9JVJ6 25 -1 -
PQQKKFKAHQAMLAALKVG Q9K0J1 25 -1 -
PQEKRRKEREKLVSGVKKG Y585_RICPR 55 -1 -
PQRQQQKKHKEMIEGLTKG YF51_HELPJ 28 -1 -
PQKKERERHRKFLESLKKG YC54_AQUAE 31 -1 -
PQMKRQKEYKSMMDHLGCG Q9PGS4 51 -1 -
PQQKKDKEHKNLINSLVQG Y134_BUCAI 42 -1 -
PQRKEEKNKKEMIKNLKKG Y651_BORBU 41 -1 -
PQQKRAKQHRELINNIQSG Q99TL5 23 -1 -
PQRVQMKKRQEMLNAVRRG Q9KJW7 35 -1 -
PQQKRQKEHLNMLNNLKRN Q9A6U1 30 -1 -
PQRRREKQFQQMISQMKRG Y859_THEMA 46 -1 -
PEKKRKKKYDAMIDELKVN Q8XJ17 27 -1 -
PEQKRRKAMEKRKNDLAKG Y884_CHLPN 52 -1 -
TQKKQQKERQDFLNNLQPG Q47777 21 -1 -
PEQKRRQAMEKRKSELAVG Y117_CHLMU 52 -1 -
PEQKRRQAMEKRKSELAVG Y741_CHLTR 52 -1 -
RQRRSMQATIDLYNSLQPG YP88_MYCLE 24 -1 -
RQRARMTEIQKLQDSVVPG Q9AE08 23 -1 -
RQRRAMQATIDLHDSLQPG YP88_MYCTU 24 -1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
GDEVLTNGGLVGRVTKVAE Q8XFR4 59 -1 -
GDEVLTNGGLVGRVTKVAE YAJC_ECOLI 59 -1 -
GDEVLTSGGFIGRVTKVAE Q8ZC34 60 -1 -
GDTVVTGGGIVGKVLKVVD YAJC_BRUAB 58 -1 -
GDEVVTAGGILGKVTKVTD Q8XVW3 59 -1 -
GDKIITIGGLHGIVEAIED Q8Y701 42 -1 -
GDKIITIGGLHGIVEAIED Q92BI5 43 -1 -
GDKIITNGGLICEVVKPEE Q9PNK0 45 -1 -
GDQVVTGGGIVGKVTKVVD Q92Q20 59 -1 -
GDQVVTGGGIVGKVTKVVD Q9JP43 59 -1 -
GTEVLTAGGVIGKITKVTE YAJC_HAEIN 45 -1 -
GDKIVTIGGLHGTIDSLDE YC29_BACHD 43 -1 -
GDEVVTSGGIAGKVTKVAD Q9HXI0 62 -1 -
GDTVVTGGGFVGKVTKVID Q98LD5 57 -1 -
GDSVVTIGGLHGTVDSIDE YRBF_BACSU 44 -1 -
GDKIVTQGGLIVEVLKAEA YF51_HELPY 46 -1 -
GDEVLTSGGLVGRITKIAE Q9KTY8 58 -1 -
GEEVLTNSGIYGIVTKVSE Q92H78 73 -1 -
GTEVLIAGGLIGRITKVSP Q9CP36 47 -1 -
GDKVVLAAGFKGKVTRVGE Q9JVJ6 43 -1 -
GDKVVLAAGFKGKVTRVGE Q9K0J1 43 -1 -
GEEVLINSGIYGIVTKVSE Y585_RICPR 73 -1 -
GDKIVTQGGLIVEVLKAEA YF51_HELPJ 46 -1 -
GDKVITSSGIWGTVVEIGD YC54_AQUAE 49 -1 -
GDEVITAGGIAGVVSVIGD Q9PGS4 69 -1 -
GDEVITTSGLLGRIKKITK Y134_BUCAI 60 -1 -
GDKVLTIGGIFGVVKKLGD Y651_BORBU 59 -1 -
GQRITTIGGIKGTVKAVDE Q99TL5 41 -1 -
GDVIVTGGGIIGKVIKVHD Q9KJW7 53 -1 -
NDTVVLSSGMIGKIVRVED Q9A6U1 48 -1 -
GDTVVTIGGIVGKVIDIKK Y859_THEMA 64 -1 -
NDKIITRGGIIGRIVKLKD Q8XJ17 45 -1 -
KGDKVTAMGIIGTVDDIRE Y884_CHLPN 69 -2 -
GDAVVTIGGLHGVISEISS Q47777 39 -1 -
VGDKVTAMGIVGTIAEIRE Y117_CHLMU 69 -2 -
VGDKVTAMGIVGTIAEIRE Y741_CHLTR 69 -2 -
GDRVNTTSGLQATIIVVGD YP88_MYCLE 42 -1 -
GDRIVTTAGQHATVISTTA Q9AE08 41 -1 -
GERVHTTSGLEATIVAIAD YP88_MYCTU 42 -1 -