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PR01847

Identifier
VIRALFUSION  [View Relations]  [View Alignment]  
Accession
PR01847
No. of Motifs
3
Creation Date
12-MAR-2003  (UPDATE 05-JUN-2003)
Title
Viral fusion protein signature
Database References

PFAM; PF02346 Vac_Fusion
INTERPRO; IPR003436
Literature References
1. BLASCO, R. AND MOSS, B.
Extracellular vaccinia virus formation and cell-to-cell virus transmission
are prevented by deletion of the gene encoding the 37,000-dalton outer
envelope protein.
J.VIROL. 65 5910-5920 (1991).
 
2. DOMS, R.W., BLUMENTHAL, R. AND MOSS, B.
Fusion of intra- and extracellular forms of vaccinia virus with the cell
membrane. 
J.VIROL. 64 4884-4892 (1990).
 
3. DEMKOWICZ, W.A., MAA, J.S. AND ESTEBAN, M.
Identification and characterization of vaccinia virus genes encoding
proteins that are highly antigenic in animals and are immunodominant in
vaccinated humans.
J.VIROL. 66 386-398 (1992).
 
4. VAZQUEZ, M.I., RIVAS, G., CREGUT, D., SERRANO, L. AND ESTEBAN, M.
The vaccinia virus 14-kilodalton (A27L) fusion protein forms a triple
coiled-coil structure and interacts with the 21-kilodalton (A17L) virus
membrane protein through a C-terminal alpha-helix.
J.VIROL. 72 10126-10137 (1997).

Documentation
Viral fusion proteins have multiple roles in the biology of viruses. The
fusion protein is required for formation and release of the extracellular
enveloped virus and virus spread [1]. The protein is also involved in the
entry process, acting in virus-to-cell and cell-to-cell fusions [2]. An
important property of the protein is its ability to confer protection
to animals immunised with the purified protein following challenge with
lethal doses of vaccinia virus [3].
 
Sequence analysis studies on the vaccinia fusion protein reveal the
existence of four regions: an apparent structureless N-terminal region,
followed by a helical region from residues 29-37, a triple coiled region
from residues 44-72, and a leucine zipper motif at the C-terminus [4].
 
VIRALFUSION is a 3-element fingerprint that provides a signature for the
viral fusion proteins. The fingerprint was derived from an initial alignment
of 13 sequences: the motifs were drawn from the conserved C-terminal region.
Two iterations on SPTR40_20f were required to reach convergence, at which
point a true set comprising 31 sequences was identified.
Summary Information
31 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3313131
2000
123
True Positives
O11367        O57232        O70735        Q66217        
Q66272 Q83542 Q83655 Q89836
Q8V3I2 Q90188 Q91MP6 Q91UM1
Q98299 Q9DHJ6 Q9J542 Q9JF68
Q9Q8I5 Q9Q8W3 Q9QB64 Q9YIX4
Q9YN58 Q9YN59 Q9YN60 Q9YN61
Q9YN62 VFUS_CAPVK VFUS_ORFN2 VFUS_VACC6
VFUS_VACCC VFUS_VACCV VFUS_VARV
Sequence Titles
O11367      B1-41-5 PROTEIN - Molluscum contagiosum virus subtype 1 (MCVI). 
O57232 14K MEMBRANE PROTEIN - Vaccinia virus (strain Ankara).
O70735 70-KDA PROTEIN - Molluscum contagiosum virus.
Q66217 14KDA FUSION PROTEIN (PUTATIVE IMV MEMBRANE FUSION PROTEIN) - Camelpox virus (strain CP-1).
Q66272 14KDA FUSION PROTEIN - Cowpox virus (CPV).
Q83542 14 KDA FUSION PROTEIN - Ectromelia virus.
Q83655 VIRAL FUSION PROTEIN - Myxoma virus.
Q89836 HOMOLOG OF VACCINIA VIRUS CDS A27L - Variola virus, and variola minor virus.
Q8V3I2 SPV114 PUTATIVE FUSION PROTEIN - Swinepox virus.
Q90188 14 KDA FUSION PROTEIN - Monkeypox virus.
Q91MP6 LSDV117 PUTATIVE FUSION PROTEIN - lumpy skin disease virus.
Q91UM1 HYPOTHETICAL 12.6 KDA PROTEIN - Vaccinia virus.
Q98299 MC133L - Molluscum contagiosum virus subtype 1 (MCVI).
Q9DHJ6 117L PROTEIN - Yaba-like disease virus (YLDV).
Q9J542 ORF FPV191 A TYPE INCLUSION PROTEIN - Fowlpox virus (FPV).
Q9JF68 TA36L - Vaccinia virus (strain Tian Tan).
Q9Q8I5 M115L - Myxoma virus (strain Lausanne).
Q9Q8W3 GP115L - Shope fibroma virus (strain Kasza) (SFV).
Q9QB64 YB-D10L PROTEIN - Yaba monkey tumor virus.
Q9YIX4 HYPOTHETICAL 12.5 KDA PROTEIN - Cowpox virus (CPV), and Rabbitpox virus.
Q9YN58 HYPOTHETICAL 12.6 KDA PROTEIN - Cowpox virus (CPV).
Q9YN59 HYPOTHETICAL 12.6 KDA PROTEIN - Cowpox virus (CPV).
Q9YN60 HYPOTHETICAL 12.6 KDA PROTEIN (A29L) - Monkeypox virus.
Q9YN61 HYPOTHETICAL 12.5 KDA PROTEIN - Vaccinia virus.
Q9YN62 HYPOTHETICAL 14.1 KDA PROTEIN - Vaccinia virus.
VFUS_CAPVK Putative fusion protein (Protein HM2) - Capripoxvirus (strain KS-1).
VFUS_ORFN2 10 kDa fusion protein - Orf virus (strain NZ2) (OV NZ-2).
VFUS_VACC6 14 kDa fusion protein - Vaccinia virus (strain WR 65-16).
VFUS_VACCC 14 kDa fusion protein - Vaccinia virus (strain Copenhagen).
VFUS_VACCV 14 kDa fusion protein - Vaccinia virus (strain WR).
VFUS_VARV 14 kDa fusion protein - Variola virus.
Scan History
SPTR40_20f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
KITNVTTKFEQIEKCCKRNDEVLFR Q9YN62 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR VFUS_VACC6 83 83 -
KITIITTKFEQIEKCCKRNDEVLFR Q9YN58 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR Q9YN59 57 57 -
RLMVLEKLVQLTIKRQKRHNKAIRR Q83655 138 138 -
RLMVLEKLVQLTIKRQKRHNKAIRR Q9Q8I5 138 138 -
RLMVLEKLVRLTVKRQKKNNKSIHR Q9Q8W3 128 128 -
RLLVLEKLFTLLFNKWSISNDIIKR Q9QB64 94 94 -
RLLVLEKLFTLLFDKWSDSNDVIKR Q9DHJ6 91 91 -
RLLVLEKLFQTSVKECNSLKNIIKR VFUS_CAPVK 95 95 -
RVTTLAKDHTDVVNCCSTVSEGLSR Q98299 485 485 -
RLGRIEQDHVEVAKSCSIVAEAIDR Q9J542 419 419 -
RLVSLLDSYKTITDCCRETGNRLDR VFUS_ORFN2 37 37 -

Motif 2 width=13
Element Seqn Id St Int Rpt
RLENHAETLRAAM Q9YN62 81 -1 -
RLENHAETLRAAM VFUS_VACC6 107 -1 -
RLENHAETLRAAM Q9YN58 81 -1 -
RLENHAETLREAM Q9YN59 81 -1 -
RIENHLETVRKGM Q83655 162 -1 -
RIENHLETVRKGM Q9Q8I5 162 -1 -
RIENHLETVRKGM Q9Q8W3 152 -1 -
RLENHTDTIRKNM Q9QB64 118 -1 -
RLEHHTDTVRKNM Q9DHJ6 115 -1 -
RLENHIETIRKNM VFUS_CAPVK 119 -1 -
RLERHAETLRKTM Q98299 509 -1 -
RLERHADTLRHSM Q9J542 443 -1 -
RLERHLESLRKAL VFUS_ORFN2 61 -1 -

Motif 3 width=14
Element Seqn Id St Int Rpt
MISLAKKIDVQTGR Q9YN62 93 -1 -
MISLAKKIDVQTGR VFUS_VACC6 119 -1 -
MISLAKKIDVQTGR Q9YN58 93 -1 -
MISLAKEIDVQTGR Q9YN59 93 -1 -
MLMLSKKIDAQTGA Q83655 174 -1 -
MLMLSKKIDAQTGA Q9Q8I5 174 -1 -
MLMLSKKIDAQTGV Q9Q8W3 164 -1 -
MIILSKKIDVQTGK Q9QB64 130 -1 -
MIILNKKIDVQTGR Q9DHJ6 127 -1 -
MIVLTKKVDFQTGR VFUS_CAPVK 131 -1 -
MLALVRKINVQTGR Q98299 521 -1 -
MVNLAKKIDYQTGY Q9J542 455 -1 -
LLDLNRKIDVQTGY VFUS_ORFN2 73 -1 -
Final Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
KITNVTTKFEQIEKCCKRNDEVLFR O57232 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR Q66217 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR Q66272 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR Q83542 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR Q91UM1 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR Q9JF68 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR Q9YIX4 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR Q9YN62 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR VFUS_VACC6 83 83 -
KITNVTTKFEQIEKCCKRNDEVLFR VFUS_VACCC 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR VFUS_VACCV 57 57 -
KITNITTKFEQIEKCCKRNDEVLFR Q90188 57 57 -
KITIITTKFEQIEKCCKRNDEVLFR Q9YN58 57 57 -
KITNVTTKFKQIEKCCKRNDEVLFR Q9YN61 57 57 -
KITNVTTKFEQIEKCCKRNDDVLFR Q89836 57 57 -
KITNVTTKFEQIEKCCKRNDDVLFR VFUS_VARV 57 57 -
KITNITTKFEQIEKCCKHNDEVLFR Q9YN60 57 57 -
KITNVTTKFEQIEKCCKRNDEVLFR Q9YN59 57 57 -
RLMVLEKLVQLTIKRQKRHNKAIRR Q83655 138 138 -
RLMVLEKLVQLTIKRQKRHNKAIRR Q9Q8I5 138 138 -
RLMVLEKLVRLTVKRQKKNNKSIHR Q9Q8W3 128 128 -
RLLVLEKLFTLLFNKWSISNDIIKR Q9QB64 94 94 -
RLLVLEKLFTLLFDKWSDSNDVIKR Q9DHJ6 91 91 -
RLLVLEKLFQTSVKECNSLKNIIKR Q91MP6 95 95 -
RLLVLEKLFQTSVKECNSLKNIIKR VFUS_CAPVK 95 95 -
RVTTLAKDHTDVVNCCSTVSEGLSR O11367 503 503 -
RVTTLAKDHTDVVNCCSTVSEGLSR O70735 485 485 -
RVTTLAKDHTDVVNCCSTVSEGLSR Q98299 485 485 -
RLGRIEQDHVEVAKSCSIVAEAIDR Q9J542 419 419 -
RLVSLLDSYKTITDCCRETGNRLDR VFUS_ORFN2 37 37 -
RLLVLEKIFQLSISNYKTLENSMKR Q8V3I2 92 92 -

Motif 2 width=13
Element Seqn Id St Int Rpt
RLENHAETLRAAM O57232 81 -1 -
RLENHAETLRAAM Q66217 81 -1 -
RLENHAETLRAAM Q66272 81 -1 -
RLENHAETLRAAM Q83542 81 -1 -
RLENHAETLRAAM Q91UM1 81 -1 -
RLENHAETLRAAM Q9JF68 81 -1 -
RLENHAETLRAAM Q9YIX4 81 -1 -
RLENHAETLRAAM Q9YN62 81 -1 -
RLENHAETLRAAM VFUS_VACC6 107 -1 -
RLENHAETLRAAM VFUS_VACCC 81 -1 -
RLENHAETLRAAM VFUS_VACCV 81 -1 -
RLENHAETLRAAM Q90188 81 -1 -
RLENHAETLRAAM Q9YN58 81 -1 -
RLENHAETLRAAM Q9YN61 81 -1 -
RLENHAETLRAAM Q89836 81 -1 -
RLENHAETLRAAM VFUS_VARV 81 -1 -
RLENHAETLRAAM Q9YN60 81 -1 -
RLENHAETLREAM Q9YN59 81 -1 -
RIENHLETVRKGM Q83655 162 -1 -
RIENHLETVRKGM Q9Q8I5 162 -1 -
RIENHLETVRKGM Q9Q8W3 152 -1 -
RLENHTDTIRKNM Q9QB64 118 -1 -
RLEHHTDTVRKNM Q9DHJ6 115 -1 -
RLENHIETIRKNM Q91MP6 119 -1 -
RLENHIETIRKNM VFUS_CAPVK 119 -1 -
RLERHAETLRKTM O11367 527 -1 -
RLERHAETLRKTM O70735 509 -1 -
RLERHAETLRKTM Q98299 509 -1 -
RLERHADTLRHSM Q9J542 443 -1 -
RLERHLESLRKAL VFUS_ORFN2 61 -1 -
RVENHLETIRRNM Q8V3I2 116 -1 -

Motif 3 width=14
Element Seqn Id St Int Rpt
MISLAKKIDVQTGR O57232 93 -1 -
MISLAKKIDVQTGR Q66217 93 -1 -
MISLAKKIDVQTGR Q66272 93 -1 -
MISLAKKIDVQTGR Q83542 93 -1 -
MISLAKKIDVQTGR Q91UM1 93 -1 -
MISLAKKIDVQTGR Q9JF68 93 -1 -
MISLAKKIDVQTGR Q9YIX4 93 -1 -
MISLAKKIDVQTGR Q9YN62 93 -1 -
MISLAKKIDVQTGR VFUS_VACC6 119 -1 -
MISLAKKIDVQTGR VFUS_VACCC 93 -1 -
MISLAKKIDVQTGR VFUS_VACCV 93 -1 -
MISLAKKIDVQTGR Q90188 93 -1 -
MISLAKKIDVQTGR Q9YN58 93 -1 -
MISLAKKIDVQTGR Q9YN61 93 -1 -
MISLAKKIDVQTGR Q89836 93 -1 -
MISLAKKIDVQTGR VFUS_VARV 93 -1 -
MISLAKKIDVQTGR Q9YN60 93 -1 -
MISLAKEIDVQTGR Q9YN59 93 -1 -
MLMLSKKIDAQTGA Q83655 174 -1 -
MLMLSKKIDAQTGA Q9Q8I5 174 -1 -
MLMLSKKIDAQTGV Q9Q8W3 164 -1 -
MIILSKKIDVQTGK Q9QB64 130 -1 -
MIILNKKIDVQTGR Q9DHJ6 127 -1 -
MIVLTKKVDFQTGR Q91MP6 131 -1 -
MIVLTKKVDFQTGR VFUS_CAPVK 131 -1 -
MLALVRKINVQTGR O11367 539 -1 -
MLALVRKINVQTGR O70735 521 -1 -
MLALVRKINVQTGR Q98299 521 -1 -
MVNLAKKIDYQTGY Q9J542 455 -1 -
LLDLNRKIDVQTGY VFUS_ORFN2 73 -1 -
MITLTKKIDVQTGR Q8V3I2 128 -1 -