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PR01845

Identifier
WNT6PROTEIN  [View Relations]  [View Alignment]  
Accession
PR01845
No. of Motifs
3
Creation Date
08-JAN-2003
Title
Wnt-6 protein signature
Database References
PRINTS; PR01349 WNTPROTEIN
MIM; 604663
Literature References
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
 
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
 
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.BRAIN RES.REV. 33 1-12 (2000).
 
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
 
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
 
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
 
7. GAVIN, B.J., MCMAHON, J.A. AND MCMAHON, A.P.
Expression of multiple novel Wnt-1/int-1-related genes during fetal and
adult mouse development.
GENES DEV. 4 2319-2332 (1990).
 
8. ITARANTA, P., YANFENG, L., PERASAARI, J., ROEL, G., DESTREE, O. AND 
VAINIO, S.
Wnt-6 is expressed in the ureter bud and induces kidney tubule development
in vitro.
GENESIS 32 259-268 (2002).

Documentation
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidneys,
regulation of mammary gland proliferation and Alzheimer's disease [3,4].
 
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of 
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been 
identified in vertebrates, with multiple subtypes within some classes.
 
Wnt-6 cDNA was isolated from mouse using a PCR-based strategy, where it
was found to be expressed in adult tissues, particularly in brain and lung
[7]. Furthermore, Wnt-6 is expressed in the ureter bud, where it is believed
to activate kidney tubule development [8].
 
WNT6PROTEIN is a 3-element fingerprint that provides a signature for Wnt-6 
proteins. The fingerprint was derived from an initial alignment of 2
sequences: the motifs were drawn from conserved regions spanning the N-
terminal half of the alignment, focusing on those sections that characterise
Wnt-6 proteins but distinguish them from related Wnt subtypes - motif 1 lies
near the N-terminus, preceded by the putative signal peptide; and motifs 2 
and 3 reside in regions between conserved cysteine residues. A single 
iteration on SPTR40_20f was required to reach convergence, no further 
sequences being identified beyond the starting set.
Summary Information
2 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3222
2000
123
True Positives
WNT6_HUMAN    WNT6_MOUSE    
Sequence Titles
WNT6_HUMAN  WNT-6 protein precursor - Homo sapiens (Human). 
WNT6_MOUSE WNT-6 protein precursor - Mus musculus (Mouse).
Scan History
SPTR40_20f 1  300  NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
SPLVMDPTSIC WNT6_HUMAN 31 31 -
SPLVMDPTSIC WNT6_MOUSE 30 30 -

Motif 2 width=12
Element Seqn Id St Int Rpt
SSHSKAFGRILQ WNT6_HUMAN 88 46 -
SSHSKAFGRVLQ WNT6_MOUSE 87 46 -

Motif 3 width=26
Element Seqn Id St Int Rpt
PRPSGLPGTPGPPGPAGSPEGSAAWE WNT6_HUMAN 143 43 -
PRPSGLLGTPGPPGPTGSPDASAAWE WNT6_MOUSE 142 43 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
SPLVMDPTSIC WNT6_HUMAN 31 31 -
SPLVMDPTSIC WNT6_MOUSE 30 30 -

Motif 2 width=12
Element Seqn Id St Int Rpt
SSHSKAFGRILQ WNT6_HUMAN 88 46 -
SSHSKAFGRVLQ WNT6_MOUSE 87 46 -

Motif 3 width=26
Element Seqn Id St Int Rpt
PRPSGLPGTPGPPGPAGSPEGSAAWE WNT6_HUMAN 143 43 -
PRPSGLLGTPGPPGPTGSPDASAAWE WNT6_MOUSE 142 43 -