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PR01836

Identifier
MGATPASE  [View Relations]  [View Alignment]  
Accession
PR01836
No. of Motifs
9
Creation Date
25-FEB-2003
Title
Magnesium-transporting ATPase signature
Database References

INTERPRO; IPR006415
Literature References
1. SMITH, D.L., TAO, T. AND MAGUIRE, M.E.
Membrane topology of a P-type ATPase. The MgtB magnesium transport
protein of Salmonella typhimurium.
J.BIOL.CHEM. 268 22469-22479 (1993).
 
2. FAGAN, M.J. AND SAIER, M.H.
P-type ATPases of eukaryotes and bacteria: sequence analyses and
construction of phylogenetic trees.
J.MOL.EVOL. 38 57-99 (1994).
 
3. AXELSEN, K.B. AND PALMGREN, M.G.
Evolution of Substrate Specificities in the P-type ATPase Superfamily.
J.MOL.EVOL. 46 84-101 (1998).
 
4. LUTSENKO, S. AND KAPLAN, J.H.
Organization of P-type ATPases: Significance of Structural Diversity.
BIOCHEMISTRY 34(48) 15607-15613 (1995).
 
5. MONCRIEF, M.C. AND MAGUIRE, M.E.
Magnesium transport in prokaryotes.
J.BIOL.INORGANIC CHEM. 4 523-527 (1999).

Documentation
P-type (or E1-E2-type) ATPases constitute a superfamily of cation transport
enzymes, present both in prokaryota and eukaryota, whose members mediate
membrane flux of all common biologically relevant cations [1]. The enzymes
can be divided into 4 major groups [2]: (1) Ca2+-transporting ATPases; 
(2) Na+/K+- and gastric H+/K+-transporting ATPases; (3) plasma membrane
H+-transporting ATPases (proton pumps) of plants, fungi and lower
eukaryotes; and (4) all bacterial P-type ATPases, except the Mg2+-ATPase
of Salmonella typhimurium [3,4], which is more similar to the eukaryotic
sequences.
 
P-type Mg2+ transporters have been characterised primarily in Salmonella
typhimurium [5]. These are unusual in that they mediate Mg2+ influx with,
rather than against, the Mg2+ electrochemical gradient - this has led to
the suggestion that Mg2+ transport is coupled with the transport of an 
as-yet unidentified molecule [5].
 
MGATPASE is a 9-element fingerprint that provides a signature for the
magnesium-transporting ATPases. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length. Two iterations on SPTR40_18f
were required to reach convergence, at which point a true set comprising 7 
sequences was identified.
Summary Information
7 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9777777777
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
ATMA_ECOLI    ATMA_SALTY    ATMB_SALTY    Q988T1        
Q9CG53 Q9HUY5 Q9S682
Sequence Titles
ATMA_ECOLI  Mg(2+) transport ATPase, P-type 1 (EC 3.6.3.2) - Escherichia coli, and Escherichia coli O157:H7. 
ATMA_SALTY Mg(2+) transport ATPase, P-type 1 (EC 3.6.3.2) - Salmonella typhimurium.
ATMB_SALTY Mg(2+) transport ATPase, P-type 2 (EC 3.6.3.2) - Salmonella typhimurium.
Q988T1 MG2+ TRANSPORT ATPASE - Rhizobium loti (Mesorhizobium loti).
Q9CG53 CATION-TRANSPORTING P-ATPASE - Lactococcus lactis (subsp. lactis) (Streptococcus lactis).
Q9HUY5 MG(2+) TRANSPORT ATPASE, P-TYPE 2 - Pseudomonas aeruginosa.
Q9S682 MG2+ TRANSPORTER MGTB - Salmonella typhimurium.
Scan History
SPTR40_18f 2  80   NSINGLE    
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
GVNSVSWLLIRFMLVMV Q9HUY5 286 286 -
GISRVSMLLIRFMLVMA ATMA_ECOLI 284 284 -
GVNSVSWLLIRFMLIMV Q9S682 290 290 -
GISRVSMLLIRFMLVMA ATMA_SALTY 288 288 -
GIRLFGYLMTEIMLAIV Q988T1 271 271 -

Motif 2 width=20
Element Seqn Id St Int Rpt
PVVLIINGYTKGDWWEAALF ATMA_SALTY 305 0 -
PVVLLINGFSKGDWVEASLF Q9S682 307 0 -
PIVLLINGFTKGDWTEAFMF Q9HUY5 303 0 -
PVVLLINGYTKGDWWEAALF ATMA_ECOLI 301 0 -
ILVFFANLLLHRPLIESLLF Q988T1 288 0 -

Motif 3 width=14
Element Seqn Id St Int Rpt
LNSHYQTGLKNLLD ATMA_SALTY 409 84 -
LNSSSQSGARNVMD Q9S682 411 84 -
LNSFHQSGMKNLMD Q9HUY5 407 84 -
LNSHYQTGLKNLLD ATMA_ECOLI 405 84 -
LNATLQTGLKNPLD Q988T1 392 84 -

Motif 4 width=16
Element Seqn Id St Int Rpt
KYIKMTASSNFGNVFS ATMA_SALTY 701 278 -
KYLNMTASSNFVNVFS Q9S682 706 281 -
KYLNMTASSNFGNVFS Q9HUY5 701 280 -
KYIKMTASSNFGNVFS ATMA_ECOLI 697 278 -
KYISITTSANFGNMIS Q988T1 680 274 -

Motif 5 width=12
Element Seqn Id St Int Rpt
VASAFLPFLPML ATMA_ECOLI 715 2 -
VRSAFIPFLPML Q9S682 724 2 -
VASAFIPFLPML Q9HUY5 719 2 -
VASAFLPFLPML ATMA_SALTY 719 2 -
VASLFLPFLPLL Q988T1 698 2 -

Motif 6 width=14
Element Seqn Id St Int Rpt
DKMDKEFLRKPRKW Q9S682 756 20 -
DKMDKEFLAKPRKW Q9HUY5 751 20 -
DNVDDEQIQKPQRW ATMA_ECOLI 747 20 -
DNVDEEQIQKPQRW ATMA_SALTY 751 20 -
DNVDPDQLRRPRHW Q988T1 730 20 -

Motif 7 width=17
Element Seqn Id St Int Rpt
RFMLWIGPTSSIFDITT Q9S682 776 6 -
RFMVWIGPTSSIFDITT Q9HUY5 771 6 -
RFMIFFGPISSIFDILT ATMA_ECOLI 767 6 -
RFMVFFGPISSIFDILT ATMA_SALTY 771 6 -
RFMISFGLVSSLFDFAT Q988T1 750 6 -

Motif 8 width=22
Element Seqn Id St Int Rpt
FQSGWFIEGLLSQTLVVHMLRT Q9S682 811 18 -
FQSGWFIEGLLSQTLVVHMLRT Q9HUY5 806 18 -
FQSGWFVVGLLSQTLIVHMIRT ATMA_ECOLI 802 18 -
FQSGWFVVGLLSQTLIVHMIRT ATMA_SALTY 806 18 -
FQTAWFVESLLTELAIVLIVRT Q988T1 781 14 -

Motif 9 width=22
Element Seqn Id St Int Rpt
PFSPLGAMVGLEPLPLSYFPWL Q9S682 861 28 -
PFSPLGAVVGLQPLPWEYFPWL Q9HUY5 856 28 -
PFSPLASYLQLQALPLSYFPWL ATMA_ECOLI 852 28 -
PFSPLASYLQLQALPLSYFPWL ATMA_SALTY 856 28 -
PYLPFAAWFGFVPLPLPVLVGL Q988T1 830 27 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
GVNSVSWLLIRFMLVMV Q9HUY5 286 286 -
GVNSVSWLLIRFMLIMV Q9S682 290 290 -
GISRVSMLLIRFMLVMA ATMA_ECOLI 284 284 -
GVNSVSWLLIRFMLIMV ATMB_SALTY 290 290 -
GISRVSMLLIRFMLVMA ATMA_SALTY 288 288 -
GVNSVSWVLIRFMLVMV Q9CG53 272 272 -
GIRLFGYLMTEIMLAIV Q988T1 271 271 -

Motif 2 width=20
Element Seqn Id St Int Rpt
PFVLLINGFTKGDWMEAALF Q9CG53 289 0 -
PVVLLINGFSKGDWVEASLF ATMB_SALTY 307 0 -
PVVLLINGFSKGDWVEASLF Q9S682 307 0 -
PIVLLINGFTKGDWTEAFMF Q9HUY5 303 0 -
PVVLLINGYTKGDWWEAALF ATMA_ECOLI 301 0 -
PVVLIINGYTKGDWWEAALF ATMA_SALTY 305 0 -
ILVFFANLLLHRPLIESLLF Q988T1 288 0 -

Motif 3 width=14
Element Seqn Id St Int Rpt
LNSYYQTGLKNLMD Q9CG53 393 84 -
LNSSSQSGARNVMD ATMB_SALTY 411 84 -
LNSSSQSGARNVMD Q9S682 411 84 -
LNSFHQSGMKNLMD Q9HUY5 407 84 -
LNSHYQTGLKNLLD ATMA_ECOLI 405 84 -
LNSHYQTGLKNLLD ATMA_SALTY 409 84 -
LNATLQTGLKNPLD Q988T1 392 84 -

Motif 4 width=16
Element Seqn Id St Int Rpt
KYIKMTASSNFGNMFS Q9CG53 694 287 -
KYLNMTASSNFVNVFS ATMB_SALTY 706 281 -
KYLNMTASSNFVNVFS Q9S682 706 281 -
KYLNMTASSNFGNVFS Q9HUY5 701 280 -
KYIKMTASSNFGNVFS ATMA_ECOLI 697 278 -
KYIKMTASSNFGNVFS ATMA_SALTY 701 278 -
KYISITTSANFGNMIS Q988T1 680 274 -

Motif 5 width=12
Element Seqn Id St Int Rpt
VASAFLPFLPML ATMA_ECOLI 715 2 -
VRSAFIPFLPML Q9S682 724 2 -
VASAFIPFLPML ATMB_SALTY 724 2 -
VASAFIPFLPML Q9HUY5 719 2 -
VASAFLPFLPML ATMA_SALTY 719 2 -
IASAFLPFIPML Q9CG53 712 2 -
VASLFLPFLPLL Q988T1 698 2 -

Motif 6 width=14
Element Seqn Id St Int Rpt
DKMDKEFLRKPRKW ATMB_SALTY 756 20 -
DKMDKEFLRKPRKW Q9S682 756 20 -
DKMDKEFLAKPRKW Q9HUY5 751 20 -
DNVDDEQIQKPQRW ATMA_ECOLI 747 20 -
DNVDEEQIQKPQRW ATMA_SALTY 751 20 -
DNVDEEYLVVPRKW Q9CG53 744 20 -
DNVDPDQLRRPRHW Q988T1 730 20 -

Motif 7 width=17
Element Seqn Id St Int Rpt
RFMLWIGPTSSIFDITT ATMB_SALTY 776 6 -
RFMLWIGPTSSIFDITT Q9S682 776 6 -
RFMVWIGPTSSIFDITT Q9HUY5 771 6 -
RFMIFFGPISSIFDILT ATMA_ECOLI 767 6 -
RFMVFFGPISSIFDILT ATMA_SALTY 771 6 -
KFMLWIGPTSSVFDITT Q9CG53 764 6 -
RFMISFGLVSSLFDFAT Q988T1 750 6 -

Motif 8 width=22
Element Seqn Id St Int Rpt
FQSGWFIEGLLSQTLVVHMLRT ATMB_SALTY 811 18 -
FQSGWFIEGLLSQTLVVHMLRT Q9S682 811 18 -
FQSGWFIEGLLSQTLVVHMLRT Q9HUY5 806 18 -
FQSGWFVVGLLSQTLIVHMIRT ATMA_ECOLI 802 18 -
FQSGWFVVGLLSQTLIVHMIRT ATMA_SALTY 806 18 -
FHTGWFVESMWTQTLVIHMIRT Q9CG53 813 32 -
FQTAWFVESLLTELAIVLIVRT Q988T1 781 14 -

Motif 9 width=22
Element Seqn Id St Int Rpt
PFSPLGAMVGLEPLPLSYFPWL ATMB_SALTY 861 28 -
PFSPLGAMVGLEPLPLSYFPWL Q9S682 861 28 -
PFSPLGAVVGLQPLPWEYFPWL Q9HUY5 856 28 -
PFSPLASYLQLQALPLSYFPWL ATMA_ECOLI 852 28 -
PFSPLASYLQLQALPLSYFPWL ATMA_SALTY 856 28 -
PFTSFGHSIGLMALPINFFPWL Q9CG53 863 28 -
PYLPFAAWFGFVPLPLPVLVGL Q988T1 830 27 -