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PR01821

Identifier
DAPIT  [View Relations]  [View Alignment]  
Accession
PR01821
No. of Motifs
3
Creation Date
13-FEB-2003
Title
DAPIT signature
Database References
Literature References
1. PAIVARINNE, H. AND KAINULAINEN, H.
DAPIT, a novel protein down-regulated in insulin-sensitive tissues in
streptozotocin-induced diabetes.
ACTA DIABETOL. 38(2) 83-86 (2001).

Documentation
Streptozotocin (STZ) is an analogue of glucose that destroys the pancreatic
beta-cells, resulting in marked insulin deficiency and consequently altered
glucose metabolism and a diabetic-like state. For this reason STZ-induced
diabetes is the standard experimental model for diabetic research in
animals. The major tissues where glucose metabolism is regulated by insulin
are skeletal muscle and adipose.
 
Expression analysis experiments in rats exhibiting STZ-induced diabetes
identified a novel gene with down-regulated expression in skeletal muscle.
The transcript contained an open reading frame for a 58-residue putative
protein, designated Diabetes-Associated Protein in Insulin-sensitive Tissues
(DAPIT). DAPIT was found to be expressed in all tissues subjected to 
Northern blot analysis, highest levels being found in skeletal and cardiac
muscle, both of which are insulin sensitive. DAPIT contains a sequence of
hydrophobic amino acids from residues 27-46 that is predicted to contain a
possible transmembrane region [1]. Other members of the family have been
found to be up-regulated in response to active stretch of skeletal muscle.
 
DAPIT is a 3-element fingerprint that provides a signature for DAPIT-like
proteins. The fingerprint was derived from an initial alignment of 4
sequences: the motifs were drawn from conserved regions spanning virtually
the full alignment length. A single iteration on SPTR40_20f was required to
reach convergence, no further sequences being identified beyond the starting
set.
Summary Information
4 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3444
2000
123
True Positives
Q96IX5        Q9CQK9        Q9ER48        Q9JJW3        
Sequence Titles
Q96IX5      SIMILAR TO UPREGULATED DURING SKELETAL MUSCLE GROWTH 5 - Homo sapiens (Human). 
Q9CQK9 2010301L15RIK PROTEIN - Mus musculus (Mouse).
Q9ER48 STRETCH REGULATED SKELETAL MUSCLE PROTEIN - Mus musculus (Mouse).
Q9JJW3 DAPIT PROTEIN - Rattus norvegicus (Rat).
Scan History
SPTR40_20f 1  300  NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
QFQFTGIKKYFNSYTLTGR Q9CQK9 9 9 -
QFQFTGIKKYFNSYTLTGR Q9JJW3 9 9 -
QFQFTGIKKYFNSYTLTGK Q9ER48 9 9 -
QYQFTGIKKYFNSYTLTGR Q96IX5 9 9 -

Motif 2 width=19
Element Seqn Id St Int Rpt
MNCVLATYGGIALLVLYFK Q9CQK9 28 0 -
MNCVLATYGGIALLVLYFK Q9JJW3 28 0 -
MNCVLATYGGIALLVLYFK Q9ER48 28 0 -
MNCVLATYGSIALIVLYFK Q96IX5 28 0 -

Motif 3 width=12
Element Seqn Id St Int Rpt
LRPKKTPAVKAT Q9CQK9 47 0 -
LRPKKTPAVKAT Q9JJW3 47 0 -
LRPKKTPAAKAT Q9ER48 47 0 -
LRSKKTPAVKAT Q96IX5 47 0 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
QFQFTGIKKYFNSYTLTGR Q9CQK9 9 9 -
QFQFTGIKKYFNSYTLTGR Q9JJW3 9 9 -
QFQFTGIKKYFNSYTLTGK Q9ER48 9 9 -
QYQFTGIKKYFNSYTLTGR Q96IX5 9 9 -

Motif 2 width=19
Element Seqn Id St Int Rpt
MNCVLATYGGIALLVLYFK Q9CQK9 28 0 -
MNCVLATYGGIALLVLYFK Q9JJW3 28 0 -
MNCVLATYGGIALLVLYFK Q9ER48 28 0 -
MNCVLATYGSIALIVLYFK Q96IX5 28 0 -

Motif 3 width=12
Element Seqn Id St Int Rpt
LRPKKTPAVKAT Q9CQK9 47 0 -
LRPKKTPAVKAT Q9JJW3 47 0 -
LRPKKTPAAKAT Q9ER48 47 0 -
LRSKKTPAVKAT Q96IX5 47 0 -