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PR01818

Identifier
DESMOCADHERN  [View Relations]  [View Alignment]  
Accession
PR01818
No. of Motifs
4
Creation Date
17-JAN-2003
Title
Desmosomal cadherin signature
Database References
PRINTS; PR01820 DESMOCOLLIN; PR01819 DESMOGLEIN
Literature References
1. LIAW, C.W., CANNON, C., POWER, M.D., KIBONEKA, P.K. AND RUBIN, L.L.
Identification and cloning of 2 species of cadherins in bovine endothelial
cells.
EMBO J. 9(9) 2701-2708 (1990).
 
2. GINSBERG, D., DESIMONE, D. AND GEIGER, B.
Expression of a novel cadherin (EP-cadherin) in unfertilized eggs and early
Xenopus embryos.
DEVELOPMENT 111(2) 315-325 (1991).
 
3. WALSH, F.S., BARTON, C.H., PUTT, W., MOORE, S.E., KELSELL, D., SPURR, N.
AND GOODFELLOW, P.N.
N-Cadherin gene maps to human chromosome 18 and is not linked to the
E-cadherin gene.
J.NEUROCHEM. 55(3) 805-812 (1990).
 
4. ISHII, K. AND GREEN, K.
Cadherin function: Breaking the barrier
CURR.BIOL. 11 R569-R572 (2001).
 
5. GARROD, D., MERRITT, A. AND NIE ,Z.
Desmosomal adhesion: structural basis, molecular mechanism and regulation 
MOL.MEMBR.BIOL. 19 81-94 (2002).
 
6. ANGST, B., MARCOZZI, C. AND MAGEE, A. 
The cadherin superfamily: diversity in form and function
J.CELL SCI. 114 629-641 (2001).
 
7. KING, I.A., ANGST, B.D., HUNT, D.M., KRUGER, M., ARNEMANN J. AND
BUXTON R.S. 
Hierarchical expression of desmosomal cadherins during stratified epithelial
morphogenesis in the mouse.
DIFFERENTIATION 62 83-96 (1997).

Documentation
Cadherins, first discovered in mouse teratocarcinoma cells [1], are 
structurally and functionally similar molecules [2] that take part in 
selective calcium-dependent adhesion interactions between cell surfaces
[3]. There are a number of different isoforms distributed in a tissue-
specific manner in a wide variety of organisms. Cells containing different 
cadherins tend to segregate in vitro, while those that contain the same 
cadherins tend to preferentially aggregate together. This observation is 
linked to the finding that cadherin expression causes morphological changes
involving the positional segregation of cells into layers, suggesting they 
may play an important role in the sorting of different cell types during 
morphogenesis, histogenesis and regeneration. They may also be involved in 
the regulation of tight and gap junctions, and in the control of 
intercellular spacing.
 
Structurally, cadherins comprise a number of domains: these include a
signal sequence; a propeptide of ~130 residues; an extracellular domain of
~600 residues; a single transmembrane (TM) domain; and a well-conserved
C-terminal cytoplasmic domain of ~150 residues. The extracellular domain 
can be subdivided into 5 parts, 4 of which are repeats of ~110 residues, 
and the fifth contains 4 conserved cysteines. The calcium-binding region
of cadherins is thought to be located in the extracellular domain.
 
Desmosomes are localised junctions that hold cells tightly together, common 
in tissues subject to mechanical strain (e.g., epithelia). Desmosomal        
cadherins are TM protein components of desmosomes (for review, see [4-6]),
whose extracellular cadherin repeats are responsible for adhesion and whose
intracellular regions interact with intermediate filaments via desmosomal
plaque proteins plakoglobin, plakobilin and desmoplakin [7]. They are 
believed to play a wider role in regulation of epithelial differentiation
[5]. Two sub-families of desmosomal cadherin have been identified,
desmocollin (DSC) and desmoglein (DSG). 
 
DESMOCADHERN is a 4-element fingerprint that provides a signature for the
desmosomal cadherins. The fingerprint was derived from an initial alignment
of 12 sequences: the motifs were drawn from conserved regions spanning the
full alignment length - motif 1 lies in the fourth cadherin repeat in the
N-terminal extracellular domain; motifs 2-4 lie in the C-terminal intra-
cellular domain. Two iterations on SPTR40_20f were required to reach   
convergence, at which point a true set comprising 14 sequences was 
identified. 
Summary Information
14 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
414141414
30000
20000
1234
True Positives
DSC1_BOVIN    DSC1_HUMAN    DSC1_MOUSE    DSC2_HUMAN    
DSC2_MOUSE DSC3_BOVIN DSC3_HUMAN DSC3_MOUSE
DSG1_BOVIN DSG1_HUMAN DSG2_HUMAN DSG3_HUMAN
Q9GKQ8 Q9HB01
Sequence Titles
DSC1_BOVIN  Desmocollin 1A/1B precursor (Desmosomal glycoprotein 2/3) (DG2/DG3) - Bos taurus (Bovine). 
DSC1_HUMAN Desmocollin 1A/1B precursor (Desmosomal glycoprotein 2/3) (DG2/DG3) - Homo sapiens (Human).
DSC1_MOUSE Desmocollin 1A/1B precursor - Mus musculus (Mouse).
DSC2_HUMAN Desmocollin 2A/2B precursor (Desmosomal glycoprotein II and III) (Desmocollin-3) - Homo sapiens (Human).
DSC2_MOUSE Desmocollin 2A/2B precursor (Epithelial type 2 desmocollin) - Mus musculus (Mouse).
DSC3_BOVIN Desmocollin 3A/3B precursor - Bos taurus (Bovine).
DSC3_HUMAN Desmocollin 3 precursor (Desmocollin 4) (HT-CP) - Homo sapiens (Human).
DSC3_MOUSE Desmocollin 3 precursor - Mus musculus (Mouse).
DSG1_BOVIN Desmoglein 1 precursor (Desmosomal glycoprotein 1) (DG1) (DGI) (Pemphigus foliaceus antigen) - Bos taurus (Bovine).
DSG1_HUMAN Desmoglein 1 precursor (Desmosomal glycoprotein 1) (DG1) (DGI) (Pemphigus foliaceus antigen) - Homo sapiens (Human).
DSG2_HUMAN Desmoglein 2 precursor (HDGC) - Homo sapiens (Human).
DSG3_HUMAN Desmoglein 3 precursor (130 kDa pemphigus vulgaris antigen) (PVA) - Homo sapiens (Human).
Q9GKQ8 DESMOGLEIN-1 PRECURSOR - Canis familiaris (Dog).
Q9HB01 DESMOCOLLIN 1A - Homo sapiens (Human).
Scan History
SPTR40_20f 2  50   NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
DGRSCTGTLVVFLED DSC1_BOVIN 554 554 -
VGRSCTGTLVVHLDD DSC1_HUMAN 555 555 -
AGRSCTGTLVVLLED DSC1_MOUSE 555 555 -
DGRSCTGTLGIILED DSC2_MOUSE 555 555 -
GGRTCTGTLGIILQD DSC2_HUMAN 555 555 -
EGKSCTGTLAVNIED DSC3_MOUSE 556 556 -
DDRSCTGTLAVNIED DSC3_HUMAN 555 555 -
DGRSCTGTLAVSIED DSC3_BOVIN 555 555 -
LQRTCTGTIVINLEN DSG1_BOVIN 471 471 -
LQRTCTGTININIQS DSG1_HUMAN 471 471 -
TGKTSTGTVYVRVPD DSG3_HUMAN 473 473 -
PRKTITGTVLINVED DSG2_HUMAN 476 476 -

Motif 2 width=13
Element Seqn Id St Int Rpt
GSVAGSVGCCSDR DSC1_BOVIN 855 286 -
GSLAGSVGCCSDR DSC1_HUMAN 856 286 -
GSMAGSVGCCSDR DSC1_MOUSE 848 278 -
GSAAGSVGCCSDL DSC2_MOUSE 864 294 -
GSVAGSVGCCSER DSC2_HUMAN 863 293 -
GSPAGSVGCCSEK DSC3_MOUSE 857 286 -
GSPAGSVGCCSEK DSC3_HUMAN 858 288 -
GSPAGSVGCCSEK DSC3_BOVIN 858 288 -
GSPAGSVGCCSFI DSG1_BOVIN 731 245 -
GSPAGSVGCCSFI DSG1_HUMAN 727 241 -
GSPVGSVGCCSFI DSG3_HUMAN 825 337 -
ESLNASIGCCSFI DSG2_HUMAN 804 313 -

Motif 3 width=13
Element Seqn Id St Int Rpt
QEEEGLDFLDHLE DSC1_BOVIN 868 0 -
QEEEGLEFLDHLE DSC1_HUMAN 869 0 -
QEEEGLEFLDQLE DSC1_MOUSE 861 0 -
QEEDGLEFLDHLE DSC2_MOUSE 877 0 -
QEEDGLEFLDNLE DSC2_HUMAN 876 0 -
QEEEGLDFLNNLE DSC3_MOUSE 870 0 -
QEEDGLDFLNNLE DSC3_HUMAN 871 0 -
QEEDGLDFLNNLE DSC3_BOVIN 871 0 -
GEDLDDSFLDTLG DSG1_BOVIN 744 0 -
GEDLDDSFLDTLG DSG1_HUMAN 740 0 -
ADDLDDSFLDSLG DSG3_HUMAN 838 0 -
EGELDDRFLDDLG DSG2_HUMAN 817 0 -

Motif 4 width=12
Element Seqn Id St Int Rpt
PKFRTLAKTCVK DSC1_BOVIN 881 0 -
PKFRTLAKTCIK DSC1_HUMAN 882 0 -
PKFRTLAKTCVK DSC1_MOUSE 874 0 -
PKFRTLAEVCAK DSC2_MOUSE 890 0 -
PKFRTLAEACMK DSC2_HUMAN 889 0 -
PKFLTLAKTCTK DSC3_MOUSE 883 0 -
PKFITLAEACTK DSC3_HUMAN 884 0 -
AKFAALAKTCTK DSC3_BOVIN 884 0 -
PKFKKLADISLG DSG1_BOVIN 757 0 -
PKFKKLADISLG DSG1_HUMAN 753 0 -
PKFKKLAEISLG DSG3_HUMAN 851 0 -
LKFKTLAEVCLG DSG2_HUMAN 830 0 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
DGRSCTGTLVVFLED DSC1_BOVIN 554 554 -
VGRSCTGTLVVHLDD DSC1_HUMAN 555 555 -
VGRSCTGTLVVHLDD Q9HB01 555 555 -
AGRSCTGTLVVLLED DSC1_MOUSE 555 555 -
DGRSCTGTLGIILED DSC2_MOUSE 555 555 -
GGRTCTGTLGIILQD DSC2_HUMAN 555 555 -
EGKSCTGTLAVNIED DSC3_MOUSE 556 556 -
DDRSCTGTLAVNIED DSC3_HUMAN 555 555 -
DGRSCTGTLAVSIED DSC3_BOVIN 555 555 -
LQRTCTGTIVINLEN DSG1_BOVIN 471 471 -
LQRTCTGTININIQS DSG1_HUMAN 471 471 -
LQRTCTGTINIDLQG Q9GKQ8 471 471 -
TGKTSTGTVYVRVPD DSG3_HUMAN 473 473 -
PRKTITGTVLINVED DSG2_HUMAN 476 476 -

Motif 2 width=13
Element Seqn Id St Int Rpt
GSVAGSVGCCSDR DSC1_BOVIN 855 286 -
GSLAGSVGCCSDR DSC1_HUMAN 856 286 -
GSLAGSVGCCSDR Q9HB01 856 286 -
GSMAGSVGCCSDR DSC1_MOUSE 848 278 -
GSAAGSVGCCSDL DSC2_MOUSE 864 294 -
GSVAGSVGCCSER DSC2_HUMAN 863 293 -
GSPAGSVGCCSEK DSC3_MOUSE 857 286 -
GSPAGSVGCCSEK DSC3_HUMAN 858 288 -
GSPAGSVGCCSEK DSC3_BOVIN 858 288 -
GSPAGSVGCCSFI DSG1_BOVIN 731 245 -
GSPAGSVGCCSFI DSG1_HUMAN 727 241 -
GSPAGSVGCCSFI Q9GKQ8 746 260 -
GSPVGSVGCCSFI DSG3_HUMAN 825 337 -
ESLNASIGCCSFI DSG2_HUMAN 804 313 -

Motif 3 width=13
Element Seqn Id St Int Rpt
QEEEGLDFLDHLE DSC1_BOVIN 868 0 -
QEEEGLEFLDHLE DSC1_HUMAN 869 0 -
QEEEGLEFLDHLE Q9HB01 869 0 -
QEEEGLEFLDQLE DSC1_MOUSE 861 0 -
QEEDGLEFLDHLE DSC2_MOUSE 877 0 -
QEEDGLEFLDNLE DSC2_HUMAN 876 0 -
QEEEGLDFLNNLE DSC3_MOUSE 870 0 -
QEEDGLDFLNNLE DSC3_HUMAN 871 0 -
QEEDGLDFLNNLE DSC3_BOVIN 871 0 -
GEDLDDSFLDTLG DSG1_BOVIN 744 0 -
GEDLDDSFLDTLG DSG1_HUMAN 740 0 -
GEDLDDSFLDTLG Q9GKQ8 759 0 -
ADDLDDSFLDSLG DSG3_HUMAN 838 0 -
EGELDDRFLDDLG DSG2_HUMAN 817 0 -

Motif 4 width=12
Element Seqn Id St Int Rpt
PKFRTLAKTCVK DSC1_BOVIN 881 0 -
PKFRTLAKTCIK DSC1_HUMAN 882 0 -
PKFRTLAKTCIK Q9HB01 882 0 -
PKFRTLAKTCVK DSC1_MOUSE 874 0 -
PKFRTLAEVCAK DSC2_MOUSE 890 0 -
PKFRTLAEACMK DSC2_HUMAN 889 0 -
PKFLTLAKTCTK DSC3_MOUSE 883 0 -
PKFITLAEACTK DSC3_HUMAN 884 0 -
AKFAALAKTCTK DSC3_BOVIN 884 0 -
PKFKKLADISLG DSG1_BOVIN 757 0 -
PKFKKLADISLG DSG1_HUMAN 753 0 -
PKFKKLADISLG Q9GKQ8 772 0 -
PKFKKLAEISLG DSG3_HUMAN 851 0 -
LKFKTLAEVCLG DSG2_HUMAN 830 0 -