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PR01807

Identifier
ANGIOMOTIN  [View Relations]  [View Alignment]  
Accession
PR01807
No. of Motifs
7
Creation Date
13-FEB-2003
Title
Angiomotin family signature
Database References
Literature References
1. TROYANOVSKY, B., LEVCHENKO, T., MANSSON, G., MATVIJENKO, O. AND
HOLMGREN, L. 
Angiomotin: an angiostatin binding protein that regulates endothelial cell
migration and tube formation.
J.CELL BIOL. 152(6) 1247-1254 (2001).
 
2. ZETTER, B.R.
Hold that line. Angiomotin regulates endothelial cell motility.
J.CELL BIOL. 152(6) F35-F36 (2001).
 
3. BRATT, A., WILSON, W.J., TROYANOVSKY, B., AASE, K., KESSLER, R.,
MEIR, E.G. AND HOLMGREN, L.
Angiomotin belongs to a novel protein family with conserved coiled-coil and
PDZ binding domains.
GENE 298(1) 69-77 (2002).

Documentation
Angiogenesis, the process whereby new blood vessels are formed by a
mechanism of sprouting from existing vessels, has recently been the subject
of intense research. Angiostatin, a proteolytically generated fragment of
plasminogen consisting of the first four kringle domains, is a potent
angiogenesis inhibitor. Whilst this protein has been known for some time,
until recently its mechanism of action was unknown. A functional angiostatin-
binding protein has been described that inhibits endothelial cell motility
and proliferation, key stages in angiogenesis [1]. This protein has been
named angiomotin.
 
Angiomotin was identififed via a yeast two-hybrid screen for proteins that
interact with angiostatin. It is a 72kDa cell-surface associated protein
expressed in endothelium and other tissues where angiogenesis occurs, such
as placenta and tumours. It appears that angiomotin stimulates angiogenesis
by increasing cell motility and that binding of angiostatin inhibits this
process [2]. Two paralogues of angiomotin have recently been cloned and
their amino acid sequences determined. Together with angiomotin, these
proteins form a novel family bearing little sequence similarity to other
known proteins. Sequence analysis has revealed putative coiled-coil and 
PDZ-binding domains [3].
 
ANGIOMOTIN is a 7-element fingerprint that provides a signature for the
angiomotin family. The fingerprint was derived from an initial alignment of 
4 sequences: the motifs were drawn from conserved regions in the central
portion of the alignment, motifs 1-5 residing in the putative coiled-coil
domain. Two iterations on SPTR40_20f were required to reach convergence, at
which point a true set comprising 6 sequences was identified. A single
partial match was also found, Q9H879, a human cDNA that shares a high degree
of similarity with the coiled-coil domain.
Summary Information
   6 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
76666666
60000000
50000000
40000000
30000000
20000011
1234567
True Positives
Q8VHG2        Q96F99        Q9D4H4        Q9HD27        
Q9QUS0 Q9UKB4
True Positive Partials
Codes involving 2 elements
Q9H879
Sequence Titles
Q8VHG2      ANGIOMOTIN - Mus musculus (Mouse).            
Q96F99 UNKNOWN (PROTEIN FOR MGC:16955) - Homo sapiens (Human).
Q9D4H4 4932416D09RIK PROTEIN - Mus musculus (Mouse).
Q9HD27 ANGIOMOTIN - Homo sapiens (Human).
Q9QUS0 LEMAN COILED-COIL PROTEIN VARIANT 2 - Mus musculus (Mouse).
Q9UKB4 LEMAN COILED-COIL PROTEIN - Homo sapiens (Human).

Q9H879 CDNA FLJ13892 FIS, CLONE THYRO1001656, MODERATELY SIMILAR TO HOMO SAPIENS LEMAN COILED-COIL PROTEIN (LCCP) MRNA - Homo sapiens (Human).
Scan History
SPTR40_20f 2  80   NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
SSKREALEKTMRNKMDSEM Q96F99 53 53 -
SSKREALEKAMRNKLEGEI Q9HD27 74 74 -
SSKREALEKAMRNKLEGEI Q8VHG2 229 229 -
TTKRESLDKAMRTKLEGEI Q9D4H4 415 415 -

Motif 2 width=16
Element Seqn Id St Int Rpt
DFNRDLRERLESANRR Q96F99 76 4 -
DFNRDLRERLETANKQ Q9HD27 97 4 -
DFNRDLRDRLETANKQ Q8VHG2 252 4 -
DFNRDLRDRLETANRQ Q9D4H4 438 4 -

Motif 3 width=15
Element Seqn Id St Int Rpt
KQAYVEKVERLQQAL Q96F99 166 74 -
KQVYVDKVEKMQQAL Q9HD27 187 74 -
KQVYVDKVEKMQQAL Q8VHG2 341 73 -
KQAYVEKVEKLQQAL Q9D4H4 527 73 -

Motif 4 width=18
Element Seqn Id St Int Rpt
QLQAACEKREQLELRLRT Q96F99 182 1 -
QLQAACEKREQLEHRLRT Q9HD27 203 1 -
QLQAACEKREQLEHRLRT Q8VHG2 357 1 -
QLQSACEKRGQMERRLRT Q9D4H4 543 1 -

Motif 5 width=18
Element Seqn Id St Int Rpt
ILALEADMTKWEQKYLEE Q96F99 246 46 -
ILALEADMTKWEQKYLEE Q9HD27 262 41 -
ILALEADMTKWEQKYLEE Q8VHG2 416 41 -
ILALEADMTKWEQKYLEE Q9D4H4 602 41 -

Motif 6 width=18
Element Seqn Id St Int Rpt
RQFAMDAAATAAAQRDTT Q96F99 267 3 -
RHFALDAAATVAAQRDTT Q9HD27 283 3 -
RHFALDAAATVAAQRDTT Q8VHG2 437 3 -
RHFAMSAAAAATAERDTT Q9D4H4 623 3 -

Motif 7 width=16
Element Seqn Id St Int Rpt
EKDAVIKVLQQRSRRD Q96F99 325 40 -
EKDAMIKVLQQRSRKE Q9HD27 348 47 -
EKDAMIKVLQQRSRKE Q8VHG2 502 47 -
EKDAMIKVLQQRSRKD Q9D4H4 689 48 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
SSKREALEKTMRNKMDSEM Q96F99 53 53 -
SSKREALEKTMRNKMDSEM Q9UKB4 53 53 -
SSKREALEKTMRNKMDGEM Q9QUS0 53 53 -
SSKREALEKAMRNKLEGEI Q9HD27 74 74 -
SSKREALEKAMRNKLEGEI Q8VHG2 229 229 -
TTKRESLDKAMRTKLEGEI Q9D4H4 415 415 -

Motif 2 width=16
Element Seqn Id St Int Rpt
DFNRDLRERLESANRR Q96F99 76 4 -
DFNRDLRERLESANRR Q9UKB4 76 4 -
DFNRDLRERLESANRH Q9QUS0 76 4 -
DFNRDLRERLETANKQ Q9HD27 97 4 -
DFNRDLRDRLETANKQ Q8VHG2 252 4 -
DFNRDLRDRLETANRQ Q9D4H4 438 4 -

Motif 3 width=15
Element Seqn Id St Int Rpt
KQAYVEKVERLQQAL Q96F99 166 74 -
KQAYVEKVERLQQAL Q9UKB4 166 74 -
KQAYVERVERLQQAL Q9QUS0 166 74 -
KQVYVDKVEKMQQAL Q9HD27 187 74 -
KQVYVDKVEKMQQAL Q8VHG2 341 73 -
KQAYVEKVEKLQQAL Q9D4H4 527 73 -

Motif 4 width=18
Element Seqn Id St Int Rpt
QLQAACEKREQLELRLRT Q96F99 182 1 -
QLQAACEKREQLELRLRT Q9UKB4 182 1 -
QLQAACEKREQLELRLRT Q9QUS0 182 1 -
QLQAACEKREQLEHRLRT Q9HD27 203 1 -
QLQAACEKREQLEHRLRT Q8VHG2 357 1 -
QLQSACEKRGQMERRLRT Q9D4H4 543 1 -

Motif 5 width=18
Element Seqn Id St Int Rpt
ILALEADMTKWEQKYLEE Q96F99 246 46 -
ILALEADMTKWEQKYLEE Q9UKB4 246 46 -
ILALEADMTKWEQKYLEE Q9QUS0 247 47 -
ILALEADMTKWEQKYLEE Q9HD27 262 41 -
ILALEADMTKWEQKYLEE Q8VHG2 416 41 -
ILALEADMTKWEQKYLEE Q9D4H4 602 41 -

Motif 6 width=18
Element Seqn Id St Int Rpt
RQFAMDAAATAAAQRDTT Q96F99 267 3 -
RQFAMDAAATAAAQRDTT Q9UKB4 267 3 -
RQFAMDAAATAAAQRDTT Q9QUS0 268 3 -
RHFALDAAATVAAQRDTT Q9HD27 283 3 -
RHFALDAAATVAAQRDTT Q8VHG2 437 3 -
RHFAMSAAAAATAERDTT Q9D4H4 623 3 -

Motif 7 width=16
Element Seqn Id St Int Rpt
EKDAVIKVLQQRSRRD Q96F99 325 40 -
EKDAVIKVLQQRSRRD Q9UKB4 325 40 -
EKDAVIKVPQQRSRKD Q9QUS0 326 40 -
EKDAMIKVLQQRSRKE Q9HD27 348 47 -
EKDAMIKVLQQRSRKE Q8VHG2 502 47 -
EKDAMIKVLQQRSRKD Q9D4H4 689 48 -