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PR01800

Identifier
STAPHEXOTOXN  [View Relations]  [View Alignment]  
Accession
PR01800
No. of Motifs
4
Creation Date
28-JUN-2002
Title
Staphylococcus aureus exotoxin signature
Database References

PDB; 1M4V
SCOP; 1M4V
CATH; 1M4V
Literature References
1. DINGES, M.M., ORWIN, P.M. AND SCHLIEVERT, P.M.
Exotoxins of Staphylococcus aureus. 
CLIN.MICROBIOL.REV. 13 16-34 (2000).
 
2. WILLIAMS, R.J., WARD, J.M., HENDERSON, B., POOLE, S., O'HARA, B.P., 
WILSON, M. AND NAIR, S.P.
Identification of a novel gene cluster encoding staphylococcal exotoxin-like 
proteins: characterization of the prototypic gene and its protein product, 
SET1.
INFECT.IMMUN. 68 4407-4415 (2000).
 
3. ARCUS, V.L., LANGLEY, R., PROFT, T., FRASER, J.D. AND BAKER, E.N.
The three-dimensional structure of a superantigen-like protein SET3, from a 
pathogenicity island of the Staphylococcus aureus genome.
J.BIOL.CHEM. 277(35) 32274-32281 (2002).

Documentation
Staphylococcus aureus is a well-characterised and specialised prokaryotic 
human pathogen, expressing a variety of virulence factors to enable 
successful infection of the host [1]. Symptoms usually manifest in cases of 
food poisoning, pyrogenic fever and toxic shock syndrome, and can prove 
lethal in immunocompromised patients [1]. Of all the exotoxins secreted from 
the bacterial cell, superantigens and hemolysins are amongst the most 
studied [1].
 
Recently, a novel gene cluster encoding several new superantigen exotoxin-
like virulence factors has been isolated from S.aureus [2]. Although the 
gene products elicit classic symptoms of pyrogenic fever and food poisoning 
in susceptible animal models, their overall similarity to sequences of known
superantigens and hemolysins is limited [2]. However, they do share regions 
of higher similarity in several key functional domains, leading these 
moieties to be termed Staphylococcal Exotoxin-like Enterotoxins (SETs), 15 
of which have so far been isolated from pathogenic strains [2]. 
 
The crystal structure of S.aureus Set3 enterotoxin has been resolved to 1.9A
by means of X-ray crystallography [3]. It reveals the two-fold structure 
characteristic of the superantigen superfamily: an N-terminal OB fold and 
a C-terminal beta-grasp domain, joined by an N-terminal alpha-helix [3].  
However, it differs significantly in several places. Beta-strands 6 and 7 
protrude from the molecular surface in the beta-grasp domain, unlike the 
usually smooth surface of other superantigens. Also, there appears to be 
a large, saddle-shaped positively-charged surface capable of binding 
negatively-charged molecules, such as DNA [3].
 
STAPHEXOTOXN is a 4-element fingerprint that provides a signature for the 
Staphylococcus aureus exotoxins. The fingerprint was derived from an initial
alignment of 6 sequences: the motifs were drawn from conserved regions 
spanning the N-terminal portion of the alignment - motif 2 includes the C- 
terminus of beta-strand 5; motif 3 encompasses strand 8 and the N-terminus 
of alpha-helix 4; and motif 4 encodes strand 10. Two iterations on 
SPTR40_20f were required to reach convergence, at which point a true set 
comprising 17 sequences was identified. Two partial matches were also found,  
both Staphyloccocal hypothetical proteins that failed to match motifs 1-2.
Summary Information
  17 codes involving  4 elements
0 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
417171717
30000
20022
1234
True Positives
Q932H0        Q99WG9        Q99WH3        Q99WH4        
Q99WH5 Q99WH6 Q99WH7 Q99WH8
Q99WH9 Q99WI0 Q99WI1 Q9RN32
Q9RN33 Q9ZFS3 Q9ZFS4 Q9ZFS5
Q9ZFS6
True Positive Partials
Codes involving 2 elements
Q99UU1 Q99WK7
Sequence Titles
Q932H0      Exotoxin 7 - Staphylococcus aureus (strain Mu50 / ATCC 700699). 
Q99WG9 Exotoxin 15 - Staphylococcus aureus (strain Mu50 / ATCC 700699).
Q99WH3 Exotoxin 14 - Staphylococcus aureus (strain Mu50 / ATCC 700699).
Q99WH4 Exotoxin 13 - Staphylococcus aureus (strain Mu50 / ATCC 700699).
Q99WH5 Exotoxin 12 - Staphylococcus aureus (strain Mu50 / ATCC 700699).
Q99WH6 Exotoxin 11 - Staphylococcus aureus (strain Mu50 / ATCC 700699).
Q99WH7 Exotoxin 10 - Staphylococcus aureus (strain Mu50 / ATCC 700699).
Q99WH8 Exotoxin 9 - Staphylococcus aureus (strain N315).
Q99WH9 Exotoxin 8 - Staphylococcus aureus (strain Mu50 / ATCC 700699).
Q99WI0 Exotoxin 7 - Staphylococcus aureus (strain N315).
Q99WI1 Exotoxin 6 - Staphylococcus aureus (strain Mu50 / ATCC 700699).
Q9RN32 Exotoxin 1 - Staphylococcus aureus.
Q9RN33 Exotoxin 1 - Staphylococcus aureus.
Q9ZFS3 Exotoxin 4 - Staphylococcus aureus.
Q9ZFS4 Exotoxin 5 - Staphylococcus aureus.
Q9ZFS5 Exotoxin 1 - Staphylococcus aureus.
Q9ZFS6 Exotoxin 3 - Staphylococcus aureus.

Q99UU1 Hypothetical protein - Staphylococcus aureus (strain Mu50 / ATCC 700699).
Q99WK7 HYPOTHETICAL PROTEIN SAV0370 - Staphylococcus aureus (strain Mu50 / ATCC 700699), and Staphylococcus aureus (strain N315).
Scan History
SPTR40_20f 2  75   NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
TGVITSEGQAVQAKE Q9RN32 18 18 -
TGVITSTAQTVNASE Q9ZFS6 18 18 -
TGTITSLHQTVNASE Q99WH7 18 18 -
TGMITTTAQPVKAST Q99WG9 18 18 -
TGVITSNVQSVQAKT Q99WI1 18 18 -
TGTLTTEAHSGHAKQ Q9ZFS3 18 18 -

Motif 2 width=12
Element Seqn Id St Int Rpt
STVGGVTKKNNK Q9RN32 117 84 -
YSYGGVTKKNQG Q9ZFS6 119 86 -
FSYGGVTKKNQD Q99WH7 119 86 -
TSIGGITKTNGT Q99WG9 112 79 -
YSIGGVTKTNSQ Q99WI1 114 81 -
YTVGGVTKTNKT Q9ZFS3 114 81 -

Motif 3 width=17
Element Seqn Id St Int Rpt
NKEEVSLKELDFKIRQH Q9RN32 156 27 -
YKEEISLKELDFKLRQY Q9ZFS6 161 30 -
YKEEISLKELDFKLRQY Q99WH7 161 30 -
YKEEISLKELDFKLRKH Q99WG9 155 31 -
YKEDISLKELDYRLRER Q99WI1 153 27 -
KKEEISLKELDFKLRKH Q9ZFS3 154 28 -

Motif 4 width=16
Element Seqn Id St Int Rpt
GEKQEIDLGDKLQFER Q9RN32 196 23 -
GGYYTFELNKKLQTNR Q9ZFS6 201 23 -
GGYYTFELNKKLQTNR Q99WH7 201 23 -
GGYYTFELNKKLQPHR Q99WG9 195 23 -
GKSHTIDLSQKLEKER Q99WI1 193 23 -
GSFYNLDLRTKLKFKH Q9ZFS3 194 23 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
TGVITSEGQAVHAKE Q99WH6 18 18 -
TGVITSEGQAVQAKE Q9RN32 18 18 -
TGVITSEGQAVQAAE Q9ZFS5 18 18 -
TGAITVTTQSVKAEK Q99WH9 18 18 -
TGVITSTAQTVNASE Q9ZFS6 18 18 -
TGVITTTTQEANATT Q99WH8 18 18 -
TGVITLESQAVKAAE Q9RN33 18 18 -
TGVFTAESKAVHAKV Q99WH4 18 18 -
TGVMTAESQTVNAKV Q9ZFS4 18 18 -
TGTITSLHQTVNASE Q99WH7 18 18 -
TGMITTTAQPVKAST Q99WG9 18 18 -
TSVMITENQSVNAKG Q99WH5 18 18 -
TGVNTTTEKPVHAEK Q932H0 18 18 -
TGVNTTTEKPVHAEK Q99WI0 18 18 -
TGVITSNVQSVQAKT Q99WI1 18 18 -
TGTLTTEAHSGHAKQ Q9ZFS3 18 18 -
TGTLTTEVHSGHAKQ Q99WH3 18 18 -

Motif 2 width=12
Element Seqn Id St Int Rpt
STVGGVTKKNNK Q99WH6 117 84 -
STVGGVTKKNNK Q9RN32 117 84 -
STVGGVTKKNNK Q9ZFS5 117 84 -
YSVGGITKTNSK Q99WH9 244 211 -
YSYGGVTKKNQG Q9ZFS6 119 86 -
YSVGGITKSNSK Q99WH8 180 147 -
STVGGVTKKNNK Q9RN33 117 84 -
YSVGGITKKNVR Q99WH4 117 84 -
YSVGGITKKNVR Q9ZFS4 117 84 -
FSYGGVTKKNQD Q99WH7 119 86 -
TSIGGITKTNGT Q99WG9 112 79 -
FSVSGVTKKNVK Q99WH5 117 84 -
YSIGGITSANDK Q932H0 117 84 -
YSIGGITSANDK Q99WI0 117 84 -
YSIGGVTKTNSQ Q99WI1 114 81 -
YTVGGVTKTNKT Q9ZFS3 114 81 -
YTVGGVIQNNKT Q99WH3 114 81 -

Motif 3 width=17
Element Seqn Id St Int Rpt
NKEEVSLKELDFKIRKQ Q99WH6 156 27 -
NKEEVSLKELDFKIRQH Q9RN32 156 27 -
QKEEISLKELDFKIRQQ Q9ZFS5 156 27 -
TKEEISLKELDFKLRKQ Q99WH9 284 28 -
YKEEISLKELDFKLRQY Q9ZFS6 161 30 -
TKEQISLKELDFKLRKQ Q99WH8 220 28 -
QKEEISLKELDFKIRQQ Q9RN33 156 27 -
QKEEVSLKELDFKIRKM Q99WH4 159 30 -
QKEEVSLKELDFKIRKM Q9ZFS4 159 30 -
YKEEISLKELDFKLRQY Q99WH7 161 30 -
YKEEISLKELDFKLRKH Q99WG9 155 31 -
QKEEVSLKELDFKIRKL Q99WH5 159 30 -
VKEAMSLKEIDFKLRKY Q932H0 157 28 -
DKEAMSLKEIDFKLRKY Q99WI0 157 28 -
YKEDISLKELDYRLRER Q99WI1 153 27 -
KKEEISLKELDFKLRKH Q9ZFS3 154 28 -
KKEKITLKELDYKLRKH Q99WH3 154 28 -

Motif 4 width=16
Element Seqn Id St Int Rpt
EKKEVIDLGDKLQFER Q99WH6 196 23 -
GEKQEIDLGDKLQFER Q9RN32 196 23 -
ENKVEIDLGDKLQFER Q9ZFS5 196 23 -
GGKYTFELHKKLQEHR Q99WH9 323 22 -
GGYYTFELNKKLQTNR Q9ZFS6 201 23 -
GGKYTFELHKKLQENR Q99WH8 259 22 -
EKREVIDLSDKLEFER Q9RN33 196 23 -
EKKYVIDLSEKLSFDR Q99WH4 199 23 -
EKKHEIDLSEKLSFDR Q9ZFS4 199 23 -
GGYYTFELNKKLQTNR Q99WH7 201 23 -
GGYYTFELNKKLQPHR Q99WG9 195 23 -
ENKYEIDLSDKLDFER Q99WH5 199 23 -
YGKYTFELDKKLQEDR Q932H0 196 22 -
YGKYTFELDKKLQEDR Q99WI0 196 22 -
GKSHTIDLSQKLEKER Q99WI1 193 23 -
GSFYNLDLRTKLKFKH Q9ZFS3 194 23 -
GSFYNLDLRSKLKFKY Q99WH3 194 23 -