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PR01796

Identifier
VDCCGAMMA8  [View Relations]  [View Alignment]  
Accession
PR01796
No. of Motifs
3
Creation Date
24-JUL-2002
Title
Voltage-dependent calcium channel gamma-8 subunit signature
Database References
PRINTS; PR01792 VDCCGAMMA
Literature References
1. BITO, H., DEISSEROTH, K. AND TSIEN, R.
Ca2+-dependent regulation in neuronal gene expression. 
CURR.OPIN.NEUROBIOL. 7 419-429 (1997).
 
2. DUNLAP, K., LEUBKE, J. AND TURNER, T.
Exocytotic calcium channels in mammalian central neurons. 
TRENDS NEUROSCI. 18 89-98 (1995). 
 
3. AHLIJANIAN, M., WESTENBROEK, R. AND CATTERALL, W.
Subunit structure and localization of dihydropyridine-sensitive calcium 
channels in mammalian brain, spinal cord, and retina.
NEURON 4 819-832 (1990).
 
4. WITCHER, D., DE WAARD, M., SAKAMOTO, J., FRANZINI-ARMSTRONG, C., 
PRAGNELL, M., KAHL, S. AND CAMPBELL, K.
Subunit identification and reconstitution of the N-type Ca2+ channel complex
purified from brain.
SCIENCE 261 486-489 (1993).
 
5. BURGESS, D., GEFRIDES, L., FOREMAN, P. AND NOEBELS, J.
A cluster of three novel Ca2+ channel gamma subunit genes on chromosome 
19q13.4: evolution and expression profile of the gamma subunit gene family.
GENOMICS 71 339-350 (2001).
 
6. CHU, P., ROBERTSON, H. AND BEST, P.
Calcium channel gamma subunits provide insights into the evolution of this 
gene family.
GENE 280 37-48 (2001).

Documentation
Voltage-dependent calcium channels are a diverse family of proteins that 
encompass a variety of biological functions, including presynaptic
neurotransmitter release and protein signalling within the cell [1,2]. The
high voltage-activated (L-, N-, P-, Q- and R-type) channels comprise the 
alpha-1 subunit, which creates the pore for the import of extracellular 
calcium ions [2]. The activity of this pore is modulated by 4 tightly-
coupled subunits: an intracellular beta subunit; a transmembrane (TM) gamma 
subunit; and a disulphide-linked complex of alpha-2 and delta subunits, 
which are proteolytically cleaved from the same gene product [3,4].
 
The voltage-dependent calcium channel gamma (VDCCG) subunit family consists 
of at least 8 members, which share a number of common structural features 
[5]. Each member is predicted to possess 4 TM domains, with intracellular
N- and C-termini. The first extracellular loop contains a highly conserved
N-glycosylation site and a pair of conserved cysteine residues. The 
C-terminal 7 residues of VDCCG-2, -3, -4 and -8 are also conserved and 
contain a consensus site for phosphorylation by cAMP and cGMP-dependent
protein kinases, and a target site for binding by PDZ domain proteins [5].
 
The VDCCG-8 subunit was identified by high throughput genomic sequence 
database searching, pursuing sequences similar to VDCCG-1 to -5 [5].
Mouse and rat isoforms have been cloned. VDCCG-8 mRNA is expressed in the 
brain and testis [6].
 
VDCCGAMMA8 is a 3-element fingerprint that provides a signature for the
voltage-dependent calcium channel gamma-8 subunit proteins. The fingerprint
was derived from an initial alignment of 3 sequences: the motifs were drawn
from conserved regions in the N-terminal and extracellular loop regions, 
focusing on those sections that characterise the VDCCG-8 subunits but 
distinguish  them from other family members - motif 1 lies in the cyto-
plasmic N-terminal region; motif 2 resides in the first extracellular
loop; and motif 3 lies in the second extracellular loop. A single iteration
on SPTR40_20f was required to reach convergence, no further sequences being
identified beyond the starting set.
Summary Information
3 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3333
2000
123
True Positives
Q8VHW2        Q8VHW5        Q8WXS5        
Sequence Titles
Q8VHW2      VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-8 SUBUNIT - Mus musculus (Mouse). 
Q8VHW5 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-8 SUBUNIT - Rattus norvegicus (Rat).
Q8WXS5 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-8 SUBUNIT - Homo sapiens (Human).
Scan History
SPTR40_20f 1  300  NSINGLE    
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
MESLKRWNEERGLWCEKG Q8VHW2 1 1 -
MESLKRWNEERGLWCEKG Q8WXS5 1 1 -
MESLKRWNEERGLWCEKG Q8VHW5 1 1 -

Motif 2 width=14
Element Seqn Id St Int Rpt
TRALICNTTNLTAG Q8VHW2 47 28 -
TRALICNTTNLTAG Q8WXS5 47 28 -
TRALICNTTNLTAG Q8VHW5 47 28 -

Motif 3 width=16
Element Seqn Id St Int Rpt
SREAHCQSRSDLLKAG Q8VHW2 230 169 -
SREAHCQSRSDLLKAG Q8WXS5 232 171 -
SREAHCQSRSDLLKAG Q8VHW5 230 169 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
MESLKRWNEERGLWCEKG Q8VHW2 1 1 -
MESLKRWNEERGLWCEKG Q8WXS5 1 1 -
MESLKRWNEERGLWCEKG Q8VHW5 1 1 -

Motif 2 width=14
Element Seqn Id St Int Rpt
TRALICNTTNLTAG Q8VHW2 47 28 -
TRALICNTTNLTAG Q8WXS5 47 28 -
TRALICNTTNLTAG Q8VHW5 47 28 -

Motif 3 width=16
Element Seqn Id St Int Rpt
SREAHCQSRSDLLKAG Q8VHW2 230 169 -
SREAHCQSRSDLLKAG Q8WXS5 232 171 -
SREAHCQSRSDLLKAG Q8VHW5 230 169 -