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PR01792

Identifier
VDCCGAMMA  [View Relations]  [View Alignment]  
Accession
PR01792
No. of Motifs
3
Creation Date
25-JUL-2002
Title
Voltage-dependent calcium channel gamma subunit signature
Database References
PRINTS; PR01601 VDCCGAMMA1; PR01602 VDCCGAMMA2; PR01603 VDCCGAMMA4
PRINTS; PR01793 VDCCGAMMA5; PR01794 VDCCGAMMA6; PR01795 VDCCGAMMA7
PRINTS; PR01796 VDCCGAMMA8
Literature References
1. BITO, H., DEISSEROTH, K. AND TSIEN, R.
Ca2+-dependent regulation in neuronal gene expression. 
CURR.OPIN.NEUROBIOL. 7 419-429 (1997).
 
2. DUNLAP, K., LEUBKE, J. AND TURNER, T.
Exocytotic calcium channels in mammalian central neurons. 
TRENDS NEUROSCI. 18 89-98 (1995). 
 
3. AHLIJANIAN, M., WESTENBROEK, R. AND CATTERALL, W.
Subunit structure and localization of dihydropyridine-sensitive calcium 
channels in mammalian brain, spinal cord, and retina.
NEURON 4 819-832 (1990).
 
4. WITCHER, D., DE WAARD, M., SAKAMOTO, J., FRANZINI-ARMSTRONG, C., 
PRAGNELL, M., KAHL, S. AND CAMPBELL, K.
Subunit identification and reconstitution of the N-type Ca2+ channel complex
purified from brain.
SCIENCE 261 486-489 (1993).
 
5. BURGESS, D., GEFRIDES, L., FOREMAN, P. AND NOEBELS, J.
A cluster of three novel Ca2+ channel gamma subunit genes on chromosome 
19q13.4: evolution and expression profile of the gamma subunit gene family.
GENOMICS 71 339-350 (2001).

Documentation
Voltage-dependent calcium channels are a diverse family of proteins that 
encompass a variety of biological functions, including presynaptic
neurotransmitter release and protein signalling within the cell [1,2]. The
high voltage-activated (L-, N-, P-, Q- and R-type) channels comprise the 
alpha-1 subunit, which creates the pore for the import of extracellular 
calcium ions [2]. The activity of this pore is modulated by 4 tightly-
coupled subunits: an intracellular beta subunit; a transmembrane (TM) gamma
subunit; and a disulphide-linked complex of alpha-2 and delta subunits, 
which are proteolytically cleaved from the same gene product [3,4].
 
The voltage-dependent calcium channel gamma (VDCCG) subunit family consists 
of at least 8 members, which share a number of common structural features 
[5]. Each member is predicted to possess 4 TM domains, with intracellular
N- and C-termini. The first extracellular loop contains a highly conserved
N-glycosylation site and a pair of conserved cysteine residues. The 
C-terminal 7 residues of VDCCG-2, -3, -4 and -8 are also conserved and 
contain a consensus site for phosphorylation by cAMP and cGMP-dependent
protein kinases, and a target site for binding by PDZ domain proteins [5].
 
VDCCGAMMA is a 3-element fingerprint that provides a signature for the
voltage-dependent calcium channel gamma subunit proteins. The fingerprint
was derived from an initial alignment of 3 sequences: the motifs were drawn
from conserved regions spanning virtually the full alignment length - motif 
1 spans the C-terminus of TM domain 1 and the N-terminus of the first
extracellular loop; motif 2 encodes the C-terminus of TM domain 3 and the N-
terminus of the second extracellular loop; and motif 3 lies partly within 
the second extracellular loop and partly within TM domain 4. Three
iterations on SPTR40_20f were required to reach convergence, at which point
a true set comprising 28 sequences was identified. A single partial match 
was also found, Q9D563, an uncharacterised translated mouse cDNA that 
matches motifs 1 and 3.
Summary Information
  28 codes involving  3 elements
1 codes involving 2 elements
Composite Feature Index
3282828
2101
123
True Positives
CCG1_HUMAN    CCG1_MOUSE    CCG1_RABIT    CCG1_RAT      
CCG2_HUMAN CCG2_MOUSE CCG3_HUMAN CCG4_HUMAN
CCG4_MOUSE CCG5_HUMAN Q8VBX3 Q8VHW2
Q8VHW3 Q8VHW4 Q8VHW5 Q8VHW6
Q8VHW7 Q8VHW8 Q8VHW9 Q8VHX0
Q8WXS5 Q8WXS6 Q8WXS7 Q90X18
Q98UH4 Q99PR9 Q9BXT2 Q9JJV5
True Positive Partials
Codes involving 2 elements
Q9D563
Sequence Titles
CCG1_HUMAN  Voltage-dependent calcium channel gamma-1 subunit (Dihydropyridine- sensitive L-type, skeletal muscle calcium channel gamma subunit) - Homo sapiens (Human). 
CCG1_MOUSE Voltage-dependent calcium channel gamma-1 subunit (Dihydropyridine- sensitive L-type, skeletal muscle calcium channel gamma subunit) - Mus musculus (Mouse).
CCG1_RABIT Voltage-dependent calcium channel gamma-1 subunit (Dihydropyridine- sensitive L-type, skeletal muscle calcium channel gamma subunit) - Oryctolagus cuniculus (Rabbit).
CCG1_RAT Voltage-dependent calcium channel gamma-1 subunit (Dihydropyridine- sensitive L-type, skeletal muscle calcium channel gamma subunit) - Rattus norvegicus (Rat).
CCG2_HUMAN Voltage-dependent calcium channel gamma-2 subunit (Neuronal voltage- gated calcium channel gamma-2 subunit) - Homo sapiens (Human).
CCG2_MOUSE Voltage-dependent calcium channel gamma-2 subunit (Neuronal voltage- gated calcium channel gamma-2 subunit) (Stargazin) - Mus musculus (Mouse).
CCG3_HUMAN Voltage-dependent calcium channel gamma-3 subunit (Neuronal voltage- gated calcium channel gamma-3 subunit) - Homo sapiens (Human).
CCG4_HUMAN Voltage-dependent calcium channel gamma-4 subunit (Neuronal voltage- gated calcium channel gamma-4 subunit) - Homo sapiens (Human).
CCG4_MOUSE Voltage-dependent calcium channel gamma-4 subunit (Neuronal voltage- gated calcium channel gamma-4 subunit) - Mus musculus (Mouse).
CCG5_HUMAN Voltage-dependent calcium channel gamma-5 subunit (Neuronal voltage- gated calcium channel gamma-5 subunit) - Homo sapiens (Human).
Q8VBX3 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-7 SUBUNIT - Mus musculus (Mouse), and Rattus norvegicus (Rat).
Q8VHW2 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-8 SUBUNIT - Mus musculus (Mouse).
Q8VHW3 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-6 SUBUNIT - Mus musculus (Mouse).
Q8VHW4 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-5 SUBUNIT - Mus musculus (Mouse).
Q8VHW5 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-8 SUBUNIT - Rattus norvegicus (Rat).
Q8VHW6 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-6 SUBUNIT SHORT ISOFORM - Rattus norvegicus (Rat).
Q8VHW7 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-6 SUBUNIT LONG ISOFORM - Rattus norvegicus (Rat).
Q8VHW8 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-5 SUBUNIT - Rattus norvegicus (Rat).
Q8VHW9 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-4 SUBUNIT - Rattus norvegicus (Rat).
Q8VHX0 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-3 SUBUNIT - Rattus norvegicus (Rat).
Q8WXS5 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-8 SUBUNIT - Homo sapiens (Human).
Q8WXS6 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-7 SUBUNIT - Homo sapiens (Human).
Q8WXS7 VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-5 SUBUNIT - Homo sapiens (Human).
Q90X18 CALCIUM CHANNEL GAMMA 4 SUBUNIT - Gallus gallus (Chicken).
Q98UH4 IPR328-LIKE PROTEIN - Gallus gallus (Chicken).
Q99PR9 IPR328 PROTEIN - Rattus norvegicus (Rat).
Q9BXT2 CALCIUM CHANNEL GAMMA SUBUNIT 6 (VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-6 SUBUNIT) - Homo sapiens (Human).
Q9JJV5 CALCIUM CHANNEL GAMMA 3 SUBUNIT - Mus musculus (Mouse).

Q9D563 4930511J11RIK PROTEIN - Mus musculus (Mouse).
Scan History
SPTR40_20f 3  300  NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
AFAAFSLMTIAVGTDYWLY CCG2_MOUSE 17 17 -
AFAAFSLMAIAIGTDYWLY CCG4_MOUSE 17 17 -
AVCGLGLLGIAVSTDYWLY CCG5_HUMAN 17 17 -
ILAGIVLAMTAVVTDHWAV CCG1_HUMAN 16 16 -

Motif 2 width=20
Element Seqn Id St Int Rpt
AGLSNIIGIIVYISANAGDP CCG2_MOUSE 145 109 -
AGLSNIIGIIVYISSNTGDP CCG4_MOUSE 148 112 -
SGLSLVVGLVLYISSINDEM CCG5_HUMAN 141 105 -
AGLCILVSVEVMRQSVKRMI CCG1_HUMAN 147 112 -

Motif 3 width=22
Element Seqn Id St Int Rpt
YSYGWSFYFGALSFIIAEMVGV CCG2_MOUSE 174 9 -
YNYGWSFYFGALSFIVAETVGV CCG4_MOUSE 179 11 -
YKYGWSFAFAAISFLLTEVKPV CCG5_HUMAN 173 12 -
YYYSWSFACACAAFILLFLGGL CCG1_HUMAN 176 9 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
AFAAFSLMTIAVGTDYWLY CCG2_HUMAN 17 17 -
AFAAFSLMTIAVGTDYWLY CCG2_MOUSE 17 17 -
AFAAFSLMTIAVGTDYWLY Q98UH4 17 17 -
AFAAFSLMTIAVGTDYWLY Q99PR9 17 17 -
AFAAFSLMTIAVGTDYWLY CCG3_HUMAN 17 17 -
AFAAFSLMTIAVGTDYWLY Q8VHX0 17 17 -
AFAAFSLMTIAVGTDYWLY Q9JJV5 17 17 -
AFAAFSLMAIAIGTDYWLY Q90X18 17 17 -
AFAAFSLMAIAIGTDYWLY CCG4_HUMAN 17 17 -
AFAAFSLMAIAIGTDYWLY CCG4_MOUSE 17 17 -
AFAAFSLMAIAIGTDYWLY Q8VHW9 17 17 -
AFAAFGLMTIAISTDYWLY Q8VHW5 28 28 -
AFAAFGLMTIAISTDYWLY Q8WXS5 28 28 -
AFSAFGLMTIAISTDYWLY Q8VHW2 28 28 -
AVCGLGLLGIAVSTDYWLY Q8VHW4 17 17 -
AVCGLGLLGIAVSTDYWLY Q8VHW8 17 17 -
AVCGLGLLGIAVSTDYWLY Q8WXS7 17 17 -
AVCGLGLLGIAVSTDYWLY CCG5_HUMAN 17 17 -
GACGLLLVGIAVSTDYWLY Q8VBX3 17 17 -
GACGLLLVGIAVSTDYWLY Q8WXS6 17 17 -
ILAGGVLAMVAVVTDHWAV CCG1_RAT 16 16 -
ILAGIVLAMTAVVTDHWAV CCG1_HUMAN 16 16 -
ILVGGVLAMVAVVTDHWAV CCG1_MOUSE 16 16 -
AIVGATLAVLAVGTEFWVE Q8VHW7 46 46 -
AIVGATLAVLAVGTEFWVE Q8VHW3 46 46 -
AIVGATLAVLAVGTEFWVE Q8VHW6 46 46 -
ILVGIVLAMTAVVSDHWAV CCG1_RABIT 16 16 -
AAVGATLAVLSVGTEFWVE Q9BXT2 46 46 -

Motif 2 width=20
Element Seqn Id St Int Rpt
AGLSNIIGIIVYISANAGDP CCG2_HUMAN 145 109 -
AGLSNIIGIIVYISANAGDP CCG2_MOUSE 145 109 -
AGLSNIIGIIVYISANAGDP Q98UH4 144 108 -
AGLSNIIGIIVYISANAGDP Q99PR9 145 109 -
AGLSNIIGIIVYISANAGDP CCG3_HUMAN 145 109 -
AGLSNIIGIIVYISANAGDP Q8VHX0 145 109 -
AGLSNIIGIIVYISANAGDP Q9JJV5 145 109 -
AGLSNIIGIIVYISSNAGDP Q90X18 149 113 -
AGLSNIIGIIVYISSNTGDP CCG4_HUMAN 148 112 -
AGLSNIIGIIVYISSNTGDP CCG4_MOUSE 148 112 -
AGLSNIIGIIVYISSNTGDP Q8VHW9 148 112 -
AGLSNIIGVIVYISANAGEP Q8VHW5 168 121 -
AGLSNIIGVIVYISANAGEP Q8WXS5 170 123 -
AGLSNIIGVIVYISANAGEP Q8VHW2 168 121 -
SGLSLVVGLVLYISSINDEM Q8VHW4 141 105 -
SGLSLVVGLVLYISSINDEM Q8VHW8 141 105 -
SGLSLVVGLVLYISSINDEM Q8WXS7 141 105 -
SGLSLVVGLVLYISSINDEM CCG5_HUMAN 141 105 -
SGLSLVVGLVLYISSINDEV Q8VBX3 141 105 -
SGLSLVVGLVLYISSINDEV Q8WXS6 141 105 -
AGLCLIVSVEVMRQSVKRMI CCG1_RAT 148 113 -
AGLCILVSVEVMRQSVKRMI CCG1_HUMAN 147 112 -
AGLCLIVSVEVMRQSVKRMI CCG1_MOUSE 148 113 -
SGLLLFVSLEVFRHSVRALL Q8VHW7 181 116 -
SGLLLFVSLEVFRHSVGALL Q8VHW3 181 116 -
KGLLLFVSLEVFRHSVRALL Q8VHW6 135 70 -
AGLCLFVSLEVMRQSVKRMI CCG1_RABIT 147 112 -
SGLLLLVSLEVFRHSVRALL Q9BXT2 181 116 -

Motif 3 width=22
Element Seqn Id St Int Rpt
YSYGWSFYFGALSFIIAEMVGV CCG2_HUMAN 174 9 -
YSYGWSFYFGALSFIIAEMVGV CCG2_MOUSE 174 9 -
YSYGWSFYFGALSFIIAEMVGV Q98UH4 172 8 -
YSYGWSFYFGALSFIIAEMVGV Q99PR9 174 9 -
YSYGWSFYFGAFSFIIAEIVGV CCG3_HUMAN 173 8 -
YSYGWSFYFGAFSFIIAEIVGV Q8VHX0 173 8 -
YSYGWSFYFGAFSFIIAEIVGV Q9JJV5 173 8 -
YNYGWSFYFGALSFIVAETIGV Q90X18 180 11 -
YNYGWSFYFGALSFIVAETVGV CCG4_HUMAN 179 11 -
YNYGWSFYFGALSFIVAETVGV CCG4_MOUSE 179 11 -
YNYGWSFYFGALSFIVAETVGV Q8VHW9 179 11 -
YSYGWSFYFGGLSFILAEVIGV Q8VHW5 199 11 -
YSYGWSFYFGGLSFILAEVIGV Q8WXS5 201 11 -
YSYGWSFYFGGLSFILAEVIGV Q8VHW2 199 11 -
YKYGWSFAFAAISFLLTESAGV Q8VHW4 173 12 -
YKYGWSFAFAAISFLLTESAGV Q8VHW8 173 12 -
YKYGWSFAFAAISFLLTESAGV Q8WXS7 173 12 -
YKYGWSFAFAAISFLLTEVKPV CCG5_HUMAN 173 12 -
YRYGWSFAFAASSFLLKEGAGV Q8VBX3 173 12 -
YRYGWSFAFAASSFLLKEGAGV Q8WXS6 173 12 -
YYYSWSFACACAGFTLLFLGGL CCG1_RAT 177 9 -
YYYSWSFACACAAFILLFLGGL CCG1_HUMAN 176 9 -
HYYSWSFACACAAFILLFLGGL CCG1_MOUSE 177 9 -
YEYSWSLGCGVGAGLILLLGGV Q8VHW7 215 14 -
YEYSWSLGCGVGAGLILLLGGV Q8VHW3 215 14 -
YEYSWSLGCGVGAGLILLLGGV Q8VHW6 169 14 -
YYYSWSFACACAAFVLLFLGGI CCG1_RABIT 176 9 -
YEYSWSLGCGVGAGLILLLGAG Q9BXT2 215 14 -