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PR01791

Identifier
REGUCALCIN  [View Relations]  [View Alignment]  
Accession
PR01791
No. of Motifs
4
Creation Date
22-AUG-2002
Title
Regucalcin signature
Database References
PRINTS; PR01790 SMP30FAMILY
Literature References
1. SHIMOKAWA, N. AND YAMAGUCHI, M.
Molecular cloning and sequencing of the cDNA coding for a calcium-binding
protein regucalcin from rat liver
FEBS LETTS. 327 251-255 (1993).
 
2. SHIMOKAWA, N. AND YAMAGUCHI, M.
Calcium administration stimulates the expression of calcium-binding protein
regucalcin mRNA in rat liver.
FEBS LETTS. 305 151-154 (1992).
 
3. YAMAGUCHI, M. AND SAKURAI, T.
Reversible effect of calcium-binding protein regucalcin on the
Ca(2+)-induced inhibition of deoxyuridine 5'-triphosphatase activity in rat
liver cytosol.
MOL.CELL BIOCHEM. 110 25-29 (1992).
 
4. MORI, S. AND YAMAGUCHI, M.
Hepatic calcium-binding protein regucalcin decreases
Ca2+/calmodulin-dependent protein kinase activity in rat liver cytosol.
CHEM.PHARM.BULL. 38 2216-2218 (1990).
 
5. KUROTA, H. AND YAMAGUCHI, M.
Inhibitory effect of calcium-binding protein regucalcin on protein kinase C
activity in rat renal cortex cytosol.
BIOL.PHARM.BULL. 21 315-318 (1998).
 
6. TAKAHASHI, H. AND YAMAGUCHI, M.
Regulatory effect of regucalcin on (Ca(2+)-Mg2+)-ATPase in rat liver plasma
membranes: comparison with the activation by Mn2+ and Co2+.
MOL.CELL BIOCHEM. 124 169-174 (1993).
 
7. YAMAGUCHI, M. AND UEOKA, S.
Inhibitory effect of calcium-binding protein regucalcin on ribonucleic acid
synthesis in isolated rat liver nuclei.
MOL.CELL BIOCHEM. 173 169-175 (1997).

Documentation
Regucalcin, also known as senesence marker protein-30 (SMP30), was
discovered in 1978 as a Ca2+ binding protein that does not contain EF-hand
motifs, suggesting a novel class of Ca2+ binding protein. It is primarily
localised to the liver and kidney cortex of animals. Expression of its mRNA 
in the liver and renal cortex of rats is stimulated by an increase in 
cellular Ca2+ levels [1,2]. 
 
Regucalin, as a regulatory protein of Ca2+, has a pivotal role in the
control of many cell functions. The protein has a reversible effect on
Ca2+-induced activation and inhibition of many enzymes in both the liver and
renal cortex cells [3]. It has also been shown to inhibit various protein
kinases (including Ca2+/calmodulin-dependent protein kinase [4], protein
kinase C [5] and tyrosine kinase) and protein phosphatases, indicating a
regulatory role in signal transduction within the cell. In addition,  
regucalcin regulates intracellular Ca2+ homeostasis by enhancing Ca2+- 
pumping activity in the plasma membrane through activation of the pump
enzymes [6]. Moreover, it can inhibit RNA synthesis in the nuclei of normal 
and regenerating rat livers in vitro [7].
 
Hydropathy profiles indicate hydrophobic domains in both N- and C-terminal 
regions of the regucalcin molecule; the protein also exhibits hydrophilic 
characteristics [1]. Human and rodent regucalcins share 89% sequence 
identity, the high degree of conservation between species suggesting that 
the complete structure is required for physiological function.
 
REGUCALCIN is a 4-element fingerprint that provides a signature for the
regucalcins. The fingerprint was derived from an initial alignment of 4 
sequences: the motifs were drawn from conserved regions spanning the central
and C-terminal portions of the alignment. Two iterations on SPTR40_20f were 
required to reach convergence, at which point a true set containing 9 
sequences was identified.
Summary Information
9 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
49999
30000
20000
1234
True Positives
Q925W3        Q9I922        Q9I923        Q9IBA8        
Q9TTJ6 SM30_BOVIN SM30_HUMAN SM30_MOUSE
SM30_RAT
Sequence Titles
Q925W3      REGUCALCIN - Rattus norvegicus (Rat).         
Q9I922 REGUCALCIN - Xenopus laevis (African clawed frog).
Q9I923 REGUCALCIN - Gallus gallus (Chicken).
Q9IBA8 SENESCENCE MARKER PROTEIN-30 - Xenopus laevis (African clawed frog).
Q9TTJ6 REGUCALCIN - Oryctolagus cuniculus (Rabbit).
SM30_BOVIN Senescence marker protein-30 (SMP-30) (Regucalcin) (RC) - Bos taurus (Bovine).
SM30_HUMAN Senescence marker protein-30 (SMP-30) (Regucalcin) (RC) - Homo sapiens (Human).
SM30_MOUSE Senescence marker protein-30 (SMP-30) (Regucalcin) (RC) - Mus musculus (Mouse).
SM30_RAT Senescence marker protein-30 (SMP-30) (Regucalcin) (RC) - Rattus norvegicus (Rat).
Scan History
SPTR40_20f 2  300  NSINGLE    
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
ERRQGSLYSLFPDH SM30_BOVIN 128 128 -
ERHQGSLYSLFPDH Q925W3 128 128 -
ERRQGSLYTLCPDH Q9I923 128 128 -
ERNQGSLFTLYPDH Q9IBA8 128 128 -

Motif 2 width=19
Element Seqn Id St Int Rpt
NRRSVYKLEKEEQIPDGMC SM30_BOVIN 189 47 -
NRRTVYKMEKDEQIPDGMC Q925W3 189 47 -
NRRSVYKLEKEESIPDGMC Q9I923 189 47 -
NRRTLYKLQQDEGIPDGMC Q9IBA8 189 47 -

Motif 3 width=14
Element Seqn Id St Int Rpt
DPETGKRLQTVKLP SM30_BOVIN 228 20 -
DPETGKRLQTVKLP Q925W3 228 20 -
DPETGKRIQTVKLP Q9I923 228 20 -
DPETGKQIQTVKLP Q9IBA8 228 20 -

Motif 4 width=17
Element Seqn Id St Int Rpt
QPEAGGIFKITGLGVKG SM30_BOVIN 275 33 -
QPDAGNIFKITGLGVKG Q925W3 275 33 -
QPQAGGVFKITGLGVKG Q9I923 275 33 -
QPQSGGIYKISGLGVKG Q9IBA8 275 33 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
ERRQGSLYSLFPDH SM30_BOVIN 128 128 -
ERHQGSLYSLFPDH Q925W3 128 128 -
ERHQGSLYSLFPDH SM30_RAT 128 128 -
ERHQGSLYSLFPDH SM30_MOUSE 128 128 -
ERHQGSLYALFPDH Q9TTJ6 128 128 -
ERHQGALYSLFPDH SM30_HUMAN 128 128 -
ERRQGSLYTLCPDH Q9I923 128 128 -
ERNQGSLFTLYPDH Q9I922 128 128 -
ERNQGSLFTLYPDH Q9IBA8 128 128 -

Motif 2 width=19
Element Seqn Id St Int Rpt
NRRSVYKLEKEEQIPDGMC SM30_BOVIN 189 47 -
NRRTVYKMEKDEQIPDGMC Q925W3 189 47 -
NRRTVYKMEKDEQIPDGMC SM30_RAT 189 47 -
NRRIVYKMEKDEQIPDGMC SM30_MOUSE 189 47 -
NRRSIYKLEKEEQIPDGMC Q9TTJ6 189 47 -
NRRSVYKLEKEEQIPDGMC SM30_HUMAN 189 47 -
NRRSVYKLEKEESIPDGMC Q9I923 189 47 -
NRRTLYKLQQDEGIPDGMC Q9I922 189 47 -
NRRTLYKLQQDEGIPDGMC Q9IBA8 189 47 -

Motif 3 width=14
Element Seqn Id St Int Rpt
DPETGKRLQTVKLP SM30_BOVIN 228 20 -
DPETGKRLQTVKLP Q925W3 228 20 -
DPETGKRLQTVKLP SM30_RAT 228 20 -
DPETGKRLQTVKLP SM30_MOUSE 228 20 -
DPETGKRLQTVKLP Q9TTJ6 228 20 -
DPVTGKRLQTVKLP SM30_HUMAN 228 20 -
DPETGKRIQTVKLP Q9I923 228 20 -
DPETGKQIQTVKLP Q9I922 228 20 -
DPETGKQIQTVKLP Q9IBA8 228 20 -

Motif 4 width=17
Element Seqn Id St Int Rpt
QPEAGGIFKITGLGVKG SM30_BOVIN 275 33 -
QPDAGNIFKITGLGVKG Q925W3 275 33 -
QPDAGNIFKITGLGVKG SM30_RAT 275 33 -
QPDAGNIFKITGLGVKG SM30_MOUSE 275 33 -
QPEAGGIFKITGLGVKG Q9TTJ6 275 33 -
QPEAGGIFKITGLGVKG SM30_HUMAN 275 33 -
QPQAGGVFKITGLGVKG Q9I923 275 33 -
QPQSGGIYKITGLGVKG Q9I922 275 33 -
QPQSGGIYKISGLGVKG Q9IBA8 275 33 -