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PR01777

Identifier
INTERFERONGR  [View Relations]  [View Alignment]  
Accession
PR01777
No. of Motifs
5
Creation Date
27-MAR-2002
Title
Interferon-gamma receptor alpha chain signature
Database References

PDB; 1FG9
SCOP; 1FG9
CATH; 1FG9
Literature References
1. WALTER, M.R., WINDSOR, W.T., NAGABHUSHAN, T.L., LUNDELL, D.J.,
LUNN, C.A., ZAUODNY, P.J. AND NARULA, S.K.
Crystal structure of a complex between interferon-gamma and its
soluble high-affinity receptor.
NATURE 376 230-235 (1995).
 
2. SOGABE, S., STUART, F., HENKE, C., BRIDGES, A., WILLIAMS, G.,
BIRCH, A., WINKLER, F.K. AND ROBINSON, J.A.
Neutralizing epitopes on the extracellular interferon gamma receptor 
X-ray crystal structure of the A6 fab-IFNgammaR1-108 complex.
J.MOL.BIOL. 273 882-897 (1997).

Documentation
Interferon (INF)-gamma is a dimeric glycoprotein produced by activated T 
cells and natural killer cells. Although originally isolated based on its
antiviral activity, INF-gamma also displays powerful anti-proliferative and
immuno-modulatory activities, which are essential for developing appropriate 
cellular defenses against a variety of infectious agents. The first step in 
eliciting these responses is the specific high affinity interaction of INF-
gamma with its cell-surface receptor (INF-gammaRalpha); the complex then
interacts with at least one of a family of additional species-specific 
accessory factors (AF-1 or INF-gammabeta), which convey different cellular 
responses. One such response is the association and phosphorylation of two 
protein tyrosine kinases (Jak-1 and Jak-2), which in turn stimulate nuclear 
transcription activators [1]. 
 
The human INF-gammaR, is a member of the hematopoietic cytokine receptor 
superfamily. It is expressed in a membrane-bound form in many cell types,
and is over-expressed in tumour cells. It comprises an extracellular portion 
of 229 residues, a single transmembrane region, and a cytoplasmic domain of 
221 residues. As with other members of its superfamily, the cytokine-binding
sites are formed by a small set of closely-spaced surface loops that extend 
from a beta-sheet core, much like antigen-binding sites on antibodies. The 
extracellular INF-gammaR monomer comprises two domains (domain D1 from 
residue 14-102, and domain D2 from residue 114-221), each resembling an Ig 
fold with fibronectin type III topology [2].
 
INGRECEPTOR is a 5-element fingerprint that provides a signature for the 
interferon-gamma receptor alpha chains. The fingerprint was derived from an 
initial alignment of 5 sequences: the motifs were drawn from conserved 
regions spanning virtually the full alignment length - motif 1 includes
beta-strand 6 in the putative extracellular domain; and motifs 2-5 span the 
cytoplasmic domain. A single iteration on SPTR39_17f was required to reach 
convergence, no further sequences being identified beyond the starting set.  
Summary Information
5 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
555555
400000
300000
200000
12345
True Positives
INGR_HUMAN    INGR_MOUSE    Q14936        Q9BY69        
Q9QZ62
Sequence Titles
INGR_HUMAN  INTERFERON-GAMMA RECEPTOR ALPHA CHAIN PRECURSOR (CDW119) - Homo sapiens (Human). 
INGR_MOUSE INTERFERON-GAMMA RECEPTOR ALPHA CHAIN PRECURSOR - Mus musculus (Mouse).
Q14936 INTERFERON-GAMMA RECEPTOR ALPHA CHAIN - Homo sapiens (Human).
Q9BY69 INTERFERON-GAMMA RECEPTOR - Homo sapiens (Human).
Q9QZ62 INTERFERON-GAMMA RECEPTOR - Rattus norvegicus (Rat).
Scan History
SPTR39_17f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
SLWVRVKARVGQKES INGR_HUMAN 97 97 -
SLWVRVKARVGQKES Q9BY69 97 97 -
SLWVRVKARVGQKES Q14936 97 97 -
SAWARVKAKVGQKES INGR_MOUSE 103 103 -
SAWARVKAKVGQRES Q9QZ62 94 94 -

Motif 2 width=15
Element Seqn Id St Int Rpt
KSIILPKSLISVVRS INGR_HUMAN 279 167 -
KSIILPKSLISVVRS Q9BY69 279 167 -
KSIILPKSLISVVRS Q14936 274 162 -
KSIMLPKSLLSVVKS INGR_MOUSE 286 168 -
KSIMLPKSLLSVVKN Q9QZ62 273 164 -

Motif 3 width=12
Element Seqn Id St Int Rpt
SATLETKPESKY INGR_HUMAN 293 -1 -
SATLETKPESKY Q9BY69 293 -1 -
SATLETKPESKY Q14936 288 -1 -
SATLETKPESKY INGR_MOUSE 300 -1 -
NATSETKPESKY Q9QZ62 287 -1 -

Motif 4 width=11
Element Seqn Id St Int Rpt
GYDKPHVLVDL INGR_HUMAN 456 151 -
GYDKPHVLVDL Q9BY69 456 151 -
GYDKPHVLVDL Q14936 451 151 -
GYDKPHMLVDV INGR_MOUSE 444 132 -
GYDKPHVLVDV Q9QZ62 431 132 -

Motif 5 width=9
Element Seqn Id St Int Rpt
GKESLIGYR INGR_HUMAN 472 5 -
GKESLIGYR Q9BY69 472 5 -
GKESLIGYR Q14936 467 5 -
GKESLMGYR INGR_MOUSE 460 5 -
GKESLIGYR Q9QZ62 447 5 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
SLWVRVKARVGQKES INGR_HUMAN 97 97 -
SLWVRVKARVGQKES Q9BY69 97 97 -
SLWVRVKARVGQKES Q14936 97 97 -
SAWARVKAKVGQKES INGR_MOUSE 103 103 -
SAWARVKAKVGQRES Q9QZ62 94 94 -

Motif 2 width=15
Element Seqn Id St Int Rpt
KSIILPKSLISVVRS INGR_HUMAN 279 167 -
KSIILPKSLISVVRS Q9BY69 279 167 -
KSIILPKSLISVVRS Q14936 274 162 -
KSIMLPKSLLSVVKS INGR_MOUSE 286 168 -
KSIMLPKSLLSVVKN Q9QZ62 273 164 -

Motif 3 width=12
Element Seqn Id St Int Rpt
SATLETKPESKY INGR_HUMAN 293 -1 -
SATLETKPESKY Q9BY69 293 -1 -
SATLETKPESKY Q14936 288 -1 -
SATLETKPESKY INGR_MOUSE 300 -1 -
NATSETKPESKY Q9QZ62 287 -1 -

Motif 4 width=11
Element Seqn Id St Int Rpt
GYDKPHVLVDL INGR_HUMAN 456 151 -
GYDKPHVLVDL Q9BY69 456 151 -
GYDKPHVLVDL Q14936 451 151 -
GYDKPHMLVDV INGR_MOUSE 444 132 -
GYDKPHVLVDV Q9QZ62 431 132 -

Motif 5 width=9
Element Seqn Id St Int Rpt
GKESLIGYR INGR_HUMAN 472 5 -
GKESLIGYR Q9BY69 472 5 -
GKESLIGYR Q14936 467 5 -
GKESLMGYR INGR_MOUSE 460 5 -
GKESLIGYR Q9QZ62 447 5 -