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PR01771

Identifier
ERK3ERK4MAPK  [View Relations]  [View Alignment]  
Accession
PR01771
No. of Motifs
9
Creation Date
18-JUL-2002
Title
ERK3/4 MAP kinase signature
Database References
Literature References
1. LEWIS, T.S., SHAPIRO, P.S. AND AHN, N.G.
Signal transduction through kinase cascades. 
ADV.CANCER RES. 74 49-139 (1998).
 
2. ERREDE, B., CADE, R.M., YASHAR, B.M., KAMADA, Y., LEVIN, D.E., IRIE, K.
AND MATSUMOTO, K.
Dynamics and organization of MAP kinases signal pathways.
MOL.REPROD.DEV. 42 477-485 (1995).
 
3. ROBINSON, M.J., XU, B.E., STIPPEC, S. AND COBB, M.H.
Different domains of the mitogen-activated protein kinases ERK3 and ERK2 
direct subcellular localization and upstream specificity in vivo. 
J.BIOL.CHEM. 277(7) 5094-5100 (2002).
 
4. PLOWMAN, G.D., SUDARSANAM, S., BINGHAM, J., WHYTE, D. AND HUNTER, T.
The protein kinases of Caenorhabditis elegans; a model for signal 
transduction in multicellular organisms. 
PROC.NATL.ACAD.SCI.U.S.A. 96 13603-13610 (1999).
 
5. HUNTER, T. AND PLOWMAN, G.D. 
The protein kinases of budding yeast: six score and more. 
TRENDS BIOCHEM.SCI. 22 18-22 (1997).
 
6. BOULTON, T.G., NYE, S.H., ROBBINS, D.J., IP, N.Y., RADZIEJEWSKA, E., 
MORGENBESSER, S.D., DEPINHO, R.A., PANAYOTATOS, N., COBB, M.H. AND 
YANCOPOULOS, G.D.
ERKs: a family of protein serine/threonine kinases are activated and 
tyrosine phosphorylated in response to insulin and NGF.
CELL 65 663-675 (1991).

Documentation
MAP (Mitogen Activated Protein) kinases participate in kinase cascades,  
whereby at least 3 protein kinases act in series, culminating in activation 
of MAP kinase [1-2]. MAP kinases are activated by dual phosphorylation 
on both tyrosine and threonine residues of a conserved TXY motif.
 
ERKs (Extracellularly Regulated Kinases) belong to the family of MAP 
kinases. ERK3 was first identified in a cDNA library using a probe for 
ERK1 [6]. ERK 3 and 4, however, have no more similarity to ERK 1 and 2 than 
do the other major classes of MAP kinase, JNK and p38. ERK3 is 
constitutively located in the nucleus, despite the lack of a traditional 
nuclear localisation signal [3]. It is unique among MAP kinases in 
containing in its activation loop only a single phosphorylation site (serine 
189) - other MAP kinases have the sequence TXY in this loop, but ERK3 
contains SEG, with glycine in place of tyrosine.  
 
ERK3 has no homologues in nematode or yeast genomes, indicating that it may 
have arisen from a relatively late gene duplication [4-5]. Its structure, 
based on similarity to ERK2, contains segregated alpha and beta regions.
 
ERK3ERK4MAPK is a 9-element fingerprint that provides a signature for the 
ERK3/4 MAP kinases. The fingerprint was derived from an initial alignment of
4 sequences: the motifs were drawn from conserved regions spanning virtually 
the full alignment length, focusing on those sections that characterise the 
ERK 3 and ERK 4 enzymes but distinguish them from the rest of the 
MAP kinase family - motif 1 includes the ATP binding site. A single 
iteration on SPTR40_20f was required to reach convergence, no further
sequences being identified beyond the starting set.
Summary Information
4 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9444444444
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
MK04_HUMAN    MK06_HUMAN    MK06_MOUSE    MK06_RAT      
Sequence Titles
MK04_HUMAN  Mitogen-activated protein kinase 4 (EC 2.7.1.-) (Extracellular signal- regulated kinase 4) (ERK-4) (MAP kinase isoform p63) (p63-MAPK) - Homo sapiens (Human). 
MK06_HUMAN Mitogen-activated protein kinase 6 (EC 2.7.1.-) (Extracellular signal- regulated kinase 3) (ERK-3) (MAP kinase isoform p97) (p97-MAPK) - Homo sapiens (Human).
MK06_MOUSE Mitogen-activated protein kinase 6 (EC 2.7.1.-) (Extracellular signal- regulated kinase 3) (ERK-3) - Mus musculus (Mouse).
MK06_RAT Mitogen-activated protein kinase 6 (EC 2.7.1.-) (Extracellular signal- regulated kinase 3) (ERK-3) (p55-MAPK) - Rattus norvegicus (Rat).
Scan History
SPTR40_20f 1  125  NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
GCGGNGLVFSAVDNDCDKR MK06_HUMAN 27 27 -
GCGGNGLVFSAVDNDCDKR MK06_RAT 27 27 -
GCGGNGLVFSAVDNDCDKR MK06_MOUSE 27 27 -
GFGVNGLVLSAVDSRACRK MK04_HUMAN 27 27 -

Motif 2 width=12
Element Seqn Id St Int Rpt
VLTDPQSVKHAL MK06_HUMAN 52 6 -
VLTDPQSVKHAL MK06_RAT 52 6 -
VLTDPQSVKHAL MK06_MOUSE 52 6 -
ALSDARSMKHAL MK04_HUMAN 52 6 -

Motif 3 width=16
Element Seqn Id St Int Rpt
KVFEILGPSGSQLTDD MK06_HUMAN 79 15 -
KVFEILGPSGSQLTDD MK06_RAT 79 15 -
KVFEILGPSGSQLTDD MK06_MOUSE 79 15 -
KVYEVLGPKGTDLQGE MK04_HUMAN 79 15 -

Motif 4 width=15
Element Seqn Id St Int Rpt
QEYMETDLANVLEQG MK06_HUMAN 108 13 -
QEYMETDLANVLEQG MK06_RAT 108 13 -
QEYMETDLANVLEQG MK06_MOUSE 108 13 -
QEYMETDLARLLEQG MK04_HUMAN 105 10 -

Motif 5 width=13
Element Seqn Id St Int Rpt
LFINTEDLVLKIG MK06_HUMAN 158 35 -
LFINTEDLVLKIG MK06_RAT 158 35 -
LFINTEDLVLKIG MK06_MOUSE 158 35 -
IFISTEDLVLKIG MK04_HUMAN 155 35 -

Motif 6 width=11
Element Seqn Id St Int Rpt
DPHYSHKGHLS MK06_HUMAN 179 8 -
DPHYSHKGHLS MK06_RAT 179 8 -
DPHYSHKGHLS MK06_MOUSE 179 8 -
DQHYSHKGYLS MK04_HUMAN 176 8 -

Motif 7 width=12
Element Seqn Id St Int Rpt
RLLLSPNNYTKA MK06_HUMAN 201 11 -
RLLLSPNNYTKA MK06_RAT 201 11 -
RLLLSPNNYTKA MK06_MOUSE 201 11 -
RLLLSPNNYTKA MK04_HUMAN 198 11 -

Motif 8 width=10
Element Seqn Id St Int Rpt
FAGAHELEQM MK06_HUMAN 232 19 -
FAGAHELEQM MK06_RAT 232 19 -
FAGAHELEQM MK06_MOUSE 232 19 -
FAGAHELEQM MK04_HUMAN 229 19 -

Motif 9 width=19
Element Seqn Id St Int Rpt
ESIPVVHEEDRQELLSVIP MK06_HUMAN 246 4 -
ESIPVVHEEDRQELLSVIP MK06_RAT 246 4 -
DSIPVVHEEDRQELLSVIP MK06_MOUSE 246 4 -
ETIPVIREEDKDELLRVMP MK04_HUMAN 243 4 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
GCGGNGLVFSAVDNDCDKR MK06_HUMAN 27 27 -
GCGGNGLVFSAVDNDCDKR MK06_RAT 27 27 -
GCGGNGLVFSAVDNDCDKR MK06_MOUSE 27 27 -
GFGVNGLVLSAVDSRACRK MK04_HUMAN 27 27 -

Motif 2 width=12
Element Seqn Id St Int Rpt
VLTDPQSVKHAL MK06_HUMAN 52 6 -
VLTDPQSVKHAL MK06_RAT 52 6 -
VLTDPQSVKHAL MK06_MOUSE 52 6 -
ALSDARSMKHAL MK04_HUMAN 52 6 -

Motif 3 width=16
Element Seqn Id St Int Rpt
KVFEILGPSGSQLTDD MK06_HUMAN 79 15 -
KVFEILGPSGSQLTDD MK06_RAT 79 15 -
KVFEILGPSGSQLTDD MK06_MOUSE 79 15 -
KVYEVLGPKGTDLQGE MK04_HUMAN 79 15 -

Motif 4 width=15
Element Seqn Id St Int Rpt
QEYMETDLANVLEQG MK06_HUMAN 108 13 -
QEYMETDLANVLEQG MK06_RAT 108 13 -
QEYMETDLANVLEQG MK06_MOUSE 108 13 -
QEYMETDLARLLEQG MK04_HUMAN 105 10 -

Motif 5 width=13
Element Seqn Id St Int Rpt
LFINTEDLVLKIG MK06_HUMAN 158 35 -
LFINTEDLVLKIG MK06_RAT 158 35 -
LFINTEDLVLKIG MK06_MOUSE 158 35 -
IFISTEDLVLKIG MK04_HUMAN 155 35 -

Motif 6 width=11
Element Seqn Id St Int Rpt
DPHYSHKGHLS MK06_HUMAN 179 8 -
DPHYSHKGHLS MK06_RAT 179 8 -
DPHYSHKGHLS MK06_MOUSE 179 8 -
DQHYSHKGYLS MK04_HUMAN 176 8 -

Motif 7 width=12
Element Seqn Id St Int Rpt
RLLLSPNNYTKA MK06_HUMAN 201 11 -
RLLLSPNNYTKA MK06_RAT 201 11 -
RLLLSPNNYTKA MK06_MOUSE 201 11 -
RLLLSPNNYTKA MK04_HUMAN 198 11 -

Motif 8 width=10
Element Seqn Id St Int Rpt
FAGAHELEQM MK06_HUMAN 232 19 -
FAGAHELEQM MK06_RAT 232 19 -
FAGAHELEQM MK06_MOUSE 232 19 -
FAGAHELEQM MK04_HUMAN 229 19 -

Motif 9 width=19
Element Seqn Id St Int Rpt
ESIPVVHEEDRQELLSVIP MK06_HUMAN 246 4 -
ESIPVVHEEDRQELLSVIP MK06_RAT 246 4 -
DSIPVVHEEDRQELLSVIP MK06_MOUSE 246 4 -
ETIPVIREEDKDELLRVMP MK04_HUMAN 243 4 -