| Literature References | 1. SUN, H., CHARLES, C.H., LAU, L.F. AND TONKS, N.K.
MKP-1 (3CH134), an immediate-early gene-product is a dual specificity
phosphatase that dephosphorylates MAP kinase in-vivo.
CELL 75(3) 487-493 (1993).
2. MUDA, M., THEODOSIOU, A., RODRIGUES, N., BOSCHERT, U., CAMPS, M.,
GILLIERON, C., DAVIES, K., ASHWORTH, A. AND ARKINSTALL, S.
The dual specificity phosphatases M3/6 and MKP-3 are highly selective for
inactivation of distinct mitogen activated protein kinases.
J.BIOL.CHEM. 271(44) 27205-27208 (1996).
3. DIXON, J.E. AND SAPER, M.A.
Crystal structure of the dual specificity protein phosphatase VHR.
SCIENCE 272 1328-1331 (1996).
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| Documentation | The dual specificity phosphatases, also known as MAP Kinase Phosphatases
(MKP), constitute a class of phosphatases that reverse the activation of MAP
(mitogen activated protein) kinases by dephosphorylating critical tyrosine
and threonine residues [1]. This regulation is mediated via interaction of a
Kinase Interaction Motif (KIM) with the common docking domain of the kinase
- this motif is shared with a number of other protein families that interact
with MAP kinases: these include kinases (MEKs), phosphatases (PTP-SL) and
transcription factors (Elk1). MKPs also share an active site motif with the
protein tyrosine phosphatases (PTPs). Different MKPs exhibit specificity
towards different members of the MAP kinase family [2].
The structure of the MPK catalytic domain is similar to that of the PTPs
[3] - the fold exhibits a complex mixed alpha-/beta-architecture. The
catalytic mechanism involves a general acid (Asp92 in 1VHR) and a catalytic
cysteine (130 in 1VHR), which acts as a nucleophile.
MAPKPHPHTASE is a 5-element fingerprint that provides a signature for the
MAP kinase phosphatases. The fingerprint was derived from an initial
alignment of 12 sequences: the motifs were drawn from conserved regions
spanning the full alignment length. Two iterations on SPTR40_20f were
required to reach convergence, at which point a true set comprising 30
sequences was identified. Several partial matches were also found, most of
which appear to be N- or C-terminal fragments.
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| Sequence Titles | DUS1_HUMAN Dual specificity protein phosphatase 1 (EC 3.1.3.48) (EC 3.1.3.16) (MAP kinase phosphatase-1) (MKP-1) (Protein-tyrosine phosphatase CL100) (Dual specificity protein phosphatase hVH1) - Homo sapiens (Human).
DUS1_MOUSE Dual specificity protein phosphatase 1 (EC 3.1.3.48) (EC 3.1.3.16) (MAP kinase phosphatase-1) (MKP-1) (Protein-tyrosine phosphatase 3CH134) (Protein-tyrosine phosphatase ERP) - Mus musculus (Mouse).
DUS1_RAT Dual specificity protein phosphatase 1 (EC 3.1.3.48) (EC 3.1.3.16) (MAP kinase phosphatase-1) (MKP-1) (Protein-tyrosine phosphatase CL100) - Rattus norvegicus (Rat).
DUS2_HUMAN Dual specificity protein phosphatase 2 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase PAC-1) - Homo sapiens (Human).
DUS2_MOUSE Dual specificity protein phosphatase 2 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase PAC-1) - Mus musculus (Mouse).
DUS4_CHICK Dual specificity protein phosphatase 4 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase-2) (MAP kinase phosphatase-2) (MKP-2) - Gallus gallus (Chicken).
DUS4_HUMAN Dual specificity protein phosphatase 4 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase hVH2) - Homo sapiens (Human).
DUS4_RAT Dual specificity protein phosphatase 4 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase-2) (MAP kinase phosphatase-2) (MKP-2) - Rattus norvegicus (Rat).
DUS5_HUMAN Dual specificity protein phosphatase 5 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase hVH3) - Homo sapiens (Human).
DUS5_RAT Dual specificity protein phosphatase 5 (EC 3.1.3.48) (EC 3.1.3.16) (MAP-kinase phosphatase CPG21) - Rattus norvegicus (Rat).
DUS6_HUMAN Dual specificity protein phosphatase 6 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase 3) (MAP kinase phosphatase 3) (MKP-3) (Dual specificity protein phosphatase PYST1) - Homo sapiens (Human).
DUS6_RAT Dual specificity protein phosphatase 6 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase 3) (MAP kinase phosphatase 3) (MKP-3) - Rattus norvegicus (Rat).
DUS8_HUMAN Dual specificity protein phosphatase 8 (EC 3.1.3.48) (EC 3.1.3.16) (Dual specificity protein phosphatase hVH-5) - Homo sapiens (Human).
DUS8_MOUSE Dual specificity protein phosphatase 8 (EC 3.1.3.48) (EC 3.1.3.16) (Neuronal tyrosine threonine phosphatase 1) - Mus musculus (Mouse).
DUS9_HUMAN Dual specificity protein phosphatase 9 (EC 3.1.3.48) (EC 3.1.3.16) (Mitogen-activated protein kinase phosphatase 4) (MAP kinase phosphatase 4) (MKP-4) - Homo sapiens (Human).
Q63683 PROTEIN TYROSINE PHOSPHATASE - Rattus norvegicus (Rat).
Q64193 3CH134/CL100 PTPASE - Rattus sp.
Q8UW48 DUAL SPECIFICITY PHOSPHATASE 6, ISOFORM A - Fugu rubripes (Japanese pufferfish) (Takifugu rubripes).
Q90W58 MAP KINASE PHOSPHATASE XCL100(BETA) PROTEIN - Xenopus laevis (African clawed frog).
Q91663 DUAL SPECIFICITY PROTEIN PHOSPHATASE ? (EC 3.1.3.48) (EC 3.1.3.16) (MAP KINASE PHOSPHATASE X17C) - Xenopus laevis (African clawed frog).
Q91790 DUAL SPECIFICITY PROTEIN PHOSPHATASE XCL100 (EC 3.1.3.48) (EC 3.1.3.16) (MAP KINASE PHOSPHATASE) - Xenopus laevis (African clawed frog).
Q920R2 MAP KINASE PHOSPHATASE-7 - Mus musculus (Mouse).
Q96N49 CDNA FLJ31411 FIS, CLONE NT2NE2000214, MODERATELY SIMILAR TO DUAL SPECIFICITY PROTEIN PHOSPHATASE 8 (EC 3.1.3.48) - Homo sapiens (Human).
Q99KC2 SIMILAR TO DUAL SPECIFICITY PHOSPHATASE 9 - Mus musculus (Mouse).
Q99MG5 MAP KINASE PHOSPHATASE-M A2 ISOFORM - Mus musculus (Mouse).
Q99MG6 MAP KINASE PHOSPHATASE-M A1 ISOFORM - Mus musculus (Mouse).
Q9AG15 MAP KINASE PHOSPHATASE-M B2 ISOFORM - Mus musculus (Mouse).
Q9AG16 MAP KINASE PHOSPHATASE-M B1 ISOFORM - Mus musculus (Mouse).
Q9CZY9 2610306G15RIK PROTEIN - Mus musculus (Mouse).
Q9ESS0 MAP KINASE PHOSPHATASE-5 - Mus musculus (Mouse).
Q8WXV0 PHOSPHATASE - Homo sapiens (Human).
Q95SV1 SD06439P - Drosophila melanogaster (Fruit fly).
Q9H9Z5 CDNA FLJ12451 FIS, CLONE NT2RM1000377, HIGHLY SIMILAR TO HOMO SAPIENS DUAL SPECIFICITY PHOSPHATASE MKP5 (MKP5) MRNA (DUAL SPECIFICITY PHOSPHATASE 10) - Homo sapiens (Human).
Q9NSW1 HYPOTHETICAL 33.0 KDA PROTEIN - Homo sapiens (Human).
Q9VVW4 CG14080 PROTEIN - Drosophila melanogaster (Fruit fly).
O44128 C05B10.1 PROTEIN - Caenorhabditis elegans.
Q96QS2 TRUNCATED MAPK PHOSPHATASE 7 - Homo sapiens (Human).
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