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PR01757

Identifier
AMELOGENIN  [View Relations]  [View Alignment]  
Accession
PR01757
No. of Motifs
4
Creation Date
11-NOV-2002  (UPDATE 02-APR-2003)
Title
Amelogenin signature
Database References

PFAM; PF02948 Amelogenin
INTERPRO; IPR004116
Literature References
1. CATALANO-SHERMAN J., LASKOV, R., PALMON, A., DAVID, S. AND DEUTSCH, D.
Production of a monoclonal antibody against human amelogenin.
CALCIF.TISSUE INT. 54 76-80 (1994).
 
2. GOTO, Y., KOGURE, E., TAKAGI, T., AIMOTO, S. AND AOBA, T.
Molecular conformation of porcine amelogenin in solution: three folding
units at the N-terminal, central, and C-terminal regions.
J.BIOCHEM. 113 55-60 (1993).
 
3. RENUGOPALAKRISHNAN, V., PRABHAKARAN, M., HUANG, S.G.,
BALASUBRAMANIAM, A., STRAWICH, E. AND GLIMCHER, M.J.
Secondary structure and limited three-dimensional structure of bovine
amelogenin.
CONNECT.TISSUE RES. 22 131-138 (1989).
 
4. LAGERSTROM-FERMER, M., NILSSON, M., BACKMAN, B., SALIDO, E., SHAPIRO, L.,
PETTERSSON, U. AND LANDEGREN, U.
Amelogenin signal peptide mutation: correlation between mutations in the
amelogenin gene (AMGX) and manifestations of X-linked amelogenesis
imperfecta.
GENOMICS 26 159-162 (1995).

Documentation
Amelogenins, cell adhesion proteins, play a role in the biomineralisation of
teeth. They seem to regulate formation of crystallites during the secretory
stage of tooth enamel development and are thought to play a major role in
the structural organisation and mineralisation of developing enamel. The
extracellular matrix of the developing enamel comprises two major classes 
of protein: the hydrophobic amelogenins and the acidic enamelins [1].
 
Circular dichroism studies of porcine amelogenin have shown that the protein
consists of 3 discrete folding units [2]: the N-terminal region appears to
contain beta-strand structures, while the C-terminal region displays
characteristics of a random coil conformation. Subsequent studies on the 
bovine protein have indicated the amelogenin structure to contain a
repetitive beta-turn segment and a "beta-spiral" between Gln112 and Leu138,
which sequester a (Pro, Leu, Gln) rich region [3]. The beta-spiral
offers a probable site for interactions with Ca2+ ions.
 
Muatations in the human amelogenin gene (AMGX) cause X-linked hypoplastic
amelogenesis imperfecta, a disease characterised by defective enamel. A 9bp
deletion in exon 2 of AMGX results in the loss of codons for Ile5, Leu6, 
Phe7 and Ala8, and replacement by a new threonine codon, disrupting
the 16-residue (Met1-Ala16) amelogenin signal peptide [4].
 
AMELOGENIN is a 4-element fingerprint that provides a signature for the
amelogenins. The fingerprint was derived from an initial alignment of 8
sequences: the motifs were drawn from conserved regions spanning virtually
the full alignment length. Two iterations on SPTR40_20f were required to
reach convergence, at which point a true set comprising 21 sequences was
identified. Several partial matches were also found, all murine or porcine
amelogenin sequences that failed to make significant matches with one or
more motifs.
Summary Information
  21 codes involving  4 elements
4 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
421212121
34323
20110
1234
True Positives
AMEL_MONDO    AMEL_MOUSE    AMEL_PIG      AMEX_BOVIN    
AMEX_HUMAN AMEY_BOVIN AMEY_HUMAN O88326
P70592 Q62945 Q63640 Q96NW6
Q9GJV6 Q9QW42 Q9TQY3 Q9TUI2
Q9TUI3 Q9YHT5 Q9YHT6 Q9YHT7
Q9Z0K9
True Positive Partials
Codes involving 3 elements
Q9QW41 Q9QW43 Q9TQY0 Q9TQY2
Codes involving 2 elements
Q9TQY1
Sequence Titles
AMEL_MONDO  Amelogenin - Monodelphis domestica (Short-tailed grey opossum). 
AMEL_MOUSE Amelogenin precursor [Contains: Leucine-rich amelogenin peptide (LRAP)] - Mus musculus (Mouse), and Rattus norvegicus (Rat).
AMEL_PIG Amelogenin precursor (Amelogenin 173A/173B) [Contains: Leucine-rich amelogenin peptide (LRAP)] - Sus scrofa (Pig).
AMEX_BOVIN Amelogenin, class I precursor - Bos taurus (Bovine).
AMEX_HUMAN Amelogenin, X isoform precursor - Homo sapiens (Human).
AMEY_BOVIN Amelogenin, class II precursor - Bos taurus (Bovine).
AMEY_HUMAN Amelogenin, Y isoform precursor - Homo sapiens (Human).
O88326 AMELOGENIN - Mesocricetus auratus (Golden hamster).
P70592 AMELOGENIN - Rattus norvegicus (Rat), and Mus musculus (Mouse).
Q62945 AMELOGENIN - Rattus norvegicus (Rat).
Q63640 AMELOGENIN - Rattus norvegicus (Rat).
Q96NW6 AMELOGENIN - Homo sapiens (Human).
Q9GJV6 AMELOGENIN PRECURSOR - Capra hircus (Goat).
Q9QW42 AMELOGENIN M74 - Mus sp.
Q9TQY3 27 KDA AMELOGENIN - Sus scrofa (Pig).
Q9TUI2 AMELOGENIN - Equus caballus (Horse).
Q9TUI3 AMELOGENIN - Equus caballus (Horse).
Q9YHT5 AMELOGENIN - Xenopus laevis (African clawed frog).
Q9YHT6 AMELOGENIN - Xenopus laevis (African clawed frog).
Q9YHT7 AMELOGENIN - Paleosuchus palpebrosus (Cuvier's dwarf caiman) (Red caiman).
Q9Z0K9 AMELOGENIN PRECURSOR - Cavia porcellus (Guinea pig).

Q9QW41 AMELOGENIN M59 - Mus sp.
Q9QW43 AMELOGENIN M141 - Mus sp.
Q9TQY0 6.5 KDA AMELOGENIN - Sus scrofa (Pig).
Q9TQY2 23 KDA AMELOGENIN - Sus scrofa (Pig).

Q9TQY1 18 KDA AMELOGENIN - Sus scrofa (Pig).
Scan History
SPTR40_20f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
PLPPHPGSPGYINLSYE AMEL_MOUSE 18 18 -
PLPPHPGSPGYINLSYE O88326 2 2 -
PLPPHPGHPGYINFSYE AMEL_PIG 18 18 -
PLPPHPGSPGYINLSYE Q63640 18 18 -
PLPPHPGHPGYINFSYE AMEY_HUMAN 18 18 -
PLPPHPGHPGYINFSYE AMEL_MONDO 2 2 -
PLPPHPHHPGYVNFSYE Q9YHT7 18 18 -
PLPPQPQHPGYVNFSYE Q9YHT5 18 18 -

Motif 2 width=15
Element Seqn Id St Int Rpt
VLTPLKWYQSMIRQP AMEL_MOUSE 35 0 -
VLTPLKWYQSMIRQP O88326 33 14 -
VLTPLKWYQNMIRHP AMEL_PIG 35 0 -
VLTPLKWYQSMIRQP Q63640 35 0 -
VLTPLKWYQSMIRPP AMEY_HUMAN 35 0 -
VLTPLKWYQSMMRHE AMEL_MONDO 19 0 -
VLTPLKWYQSLMRQP Q9YHT7 35 0 -
ILSPIKWYQSMMKNQ Q9YHT5 35 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
PQQPMMPVPGHHSMTP AMEL_MOUSE 97 47 -
PQQPMMPVPGHHSMTP O88326 95 47 -
PQQPMMPLPGQHSMTP AMEL_PIG 97 47 -
PQQPMMPVPGHHSMTP Q63640 73 23 -
RQQALMPVPGQQSMTP AMEY_HUMAN 97 47 -
PQQPVMPVPGQHPMAP AMEL_MONDO 81 47 -
PQHPLMQMPGPHQMMP Q9YHT7 90 40 -
PQQPLVPVPVHHPLIP Q9YHT5 96 46 -

Motif 4 width=21
Element Seqn Id St Int Rpt
LPELPLEAWPATDKTKREEVD AMEL_MOUSE 176 63 -
LPELPLEAWPATDKTKREEVD O88326 174 63 -
LPDLPLEAWPATDKTKREEVD AMEL_PIG 169 56 -
LPELPLEAWPATDKTKREEVA Q63640 152 63 -
LPDLHLEAWPATDKTKQEEVD AMEY_HUMAN 172 59 -
PMLPDMEAWPATDKTKREEVD AMEL_MONDO 182 85 -
LPDMPLEPWRPMDKTKQEEID Q9YHT7 179 73 -
VEERPQEPWQEAGNDKQEELD Q9YHT5 164 52 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
PLPPHPGHPGYINFSYE AMEX_BOVIN 18 18 -
PLPPHPGHPGYINFSYE Q9Z0K9 18 18 -
PLPPHPGSPGYINLSYE AMEL_MOUSE 18 18 -
PLPPHPGSPGYINLSYE P70592 18 18 -
PLPPHPGSPGYINLSYE O88326 2 2 -
PLPPHPGHPGYINFSYE AMEL_PIG 18 18 -
PLPPHPGHPGYINFSYE Q9TQY3 2 2 -
PLPPHPGSPGYINLSYE Q62945 18 18 -
PLPPHPGSPGYINLSYE Q63640 18 18 -
PLPPHPGHPGYINFSYE AMEY_BOVIN 18 18 -
PLPPHPGHPGYINFSYE Q9GJV6 18 18 -
PLPPHPGHPGYINFSYE AMEX_HUMAN 18 18 -
PLPPHPGHPGYINFSYE Q96NW6 18 18 -
PLPPHPGHPGYINFSYE AMEY_HUMAN 18 18 -
PLYLILGTLGYINFSYE Q9TUI2 18 18 -
PLYLILGTLGYINFSYE Q9TUI3 18 18 -
PLPPHPGHPGYINFSYE AMEL_MONDO 2 2 -
PLPPHPGSPGYINLSYE Q9QW42 2 2 -
PLPPHPHHPGYVNFSYE Q9YHT7 18 18 -
PLPPHPQHPGYVNFSYE Q9YHT6 18 18 -
PLPPQPQHPGYVNFSYE Q9YHT5 18 18 -

Motif 2 width=15
Element Seqn Id St Int Rpt
VLTPLKWYQSMIRHP AMEX_BOVIN 35 0 -
VLTPLKWYQSMIRQP Q9Z0K9 49 14 -
VLTPLKWYQSMIRQP AMEL_MOUSE 35 0 -
VLTPLKWYQSMIRQP P70592 49 14 -
VLTPLKWYQSMIRQP O88326 33 14 -
VLTPLKWYQNMIRHP AMEL_PIG 35 0 -
VLTPLKWYQNMIRHP Q9TQY3 36 17 -
VLTPLKWYQSMIRQP Q62945 35 0 -
VLTPLKWYQSMIRQP Q63640 35 0 -
VLTPLKWYQNMLRYP AMEY_BOVIN 35 0 -
VPTPLKWYQSMIRHP Q9GJV6 35 0 -
VLTPLKWYQSIRPPY AMEX_HUMAN 35 0 -
VLTPLKWYQSIRPPY Q96NW6 49 14 -
VLTPLKWYQSMIRPP AMEY_HUMAN 35 0 -
VLTPLKWYQSLIRQP Q9TUI2 35 0 -
VLTPLKWYQSLIRQP Q9TUI3 35 0 -
VLTPLKWYQSMMRHE AMEL_MONDO 19 0 -
VLTPLKWYQSMIRQP Q9QW42 19 0 -
VLTPLKWYQSLMRQP Q9YHT7 35 0 -
ILSPLKWYQSMMTHQ Q9YHT6 35 0 -
ILSPIKWYQSMMKNQ Q9YHT5 35 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
PQQPMMPVPGQHSMTP AMEX_BOVIN 97 47 -
PQQPLMPVPGHHSMTP Q9Z0K9 112 48 -
PQQPMMPVPGHHSMTP AMEL_MOUSE 97 47 -
PQQPMMPVPGHHSMTP P70592 111 47 -
PQQPMMPVPGHHSMTP O88326 95 47 -
PQQPMMPLPGQHSMTP AMEL_PIG 97 47 -
PQQPMMPLPGQHSMTP Q9TQY3 98 47 -
PQQPMMPVPGHHSMTP Q62945 97 47 -
PQQPMMPVPGHHSMTP Q63640 73 23 -
PQQPMMPVPGQHSMTP AMEY_BOVIN 97 47 -
PQQPMMPVPGQHSMTP Q9GJV6 97 47 -
PQQPMMPVPGQHSMTP AMEX_HUMAN 96 46 -
PQQPMMPVPGQHSMTP Q96NW6 110 46 -
RQQALMPVPGQQSMTP AMEY_HUMAN 97 47 -
PQQPMMPLPGQHSMVP Q9TUI2 97 47 -
PQQPMMPLPGQHSMVP Q9TUI3 97 47 -
PQQPVMPVPGQHPMAP AMEL_MONDO 81 47 -
PQPPLPPLFSMQPLSP Q9QW42 37 3 -
PQHPLMQMPGPHQMMP Q9YHT7 90 40 -
PQHPLVPVPVHHPVFP Q9YHT6 93 43 -
PQQPLVPVPVHHPLIP Q9YHT5 96 46 -

Motif 4 width=21
Element Seqn Id St Int Rpt
LPDLPLEAWPATDKTKREEVD AMEX_BOVIN 193 80 -
LPDLPLEAWPATDKTKREEVD Q9Z0K9 206 78 -
LPELPLEAWPATDKTKREEVD AMEL_MOUSE 176 63 -
LPELPLEAWPATDKTKREEVD P70592 190 63 -
LPELPLEAWPATDKTKREEVD O88326 174 63 -
LPDLPLEAWPATDKTKREEVD AMEL_PIG 169 56 -
LPDLPLEAWPATDKTKREEVD Q9TQY3 170 56 -
LPELPLEAWPATDKTKREEVA Q62945 176 63 -
LPELPLEAWPATDKTKREEVA Q63640 152 63 -
LPDLPLEAWPATDKTKREEVD AMEY_BOVIN 172 59 -
IPDVLLEDWPATDKTKREEVD Q9GJV6 187 74 -
LPDLTLEAWPSTDKTKREEVD AMEX_HUMAN 171 59 -
LPDLTLEAWPSTDKTKREEVD Q96NW6 185 59 -
LPDLHLEAWPATDKTKQEEVD AMEY_HUMAN 172 59 -
LPDLPLEAWPATDKTKREEVD Q9TUI2 164 51 -
LPDLPLEAWPATDKTKREEVD Q9TUI3 172 59 -
PMLPDMEAWPATDKTKREEVD AMEL_MONDO 182 85 -
LPELPLEAWPATDKTKREEVD Q9QW42 54 1 -
LPDMPLEPWRPMDKTKQEEID Q9YHT7 179 73 -
VEDRPQEPWQAAGNTKQEELD Q9YHT6 153 44 -
VEERPQEPWQEAGNDKQEELD Q9YHT5 164 52 -