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PR01756

Identifier
OMADHESIN  [View Relations]  [View Alignment]  
Accession
PR01756
No. of Motifs
5
Creation Date
27-JUN-2002
Title
Yersinia outer membrane adhesin signature
Database References
Literature References
1. IRIARTE, M. AND CORNELIS, G.R.
Molecular determinants of Yersinia pathogenesis.
MICROBIOLOGIA 12 267-280 (1996).
 
2. BLISKA, J.B., COPASS, M.C. AND FALKOW, S.
The Yersinia pseudotuberculosis adhesin YadA mediates intimate bacterial 
attachment to and entry into Hep-2 cells. 
INFECT.IMMUN. 61 3914-3921 (1993).
 
3. HOICZYK, E., ROGGENKAMP, A., REICHENBECHER, M., LUPAS, A. AND HEESEMANN, J.
Structure and sequence analysis of Yersinia YadA and Moraxella UspAs reveal  
a novel class of adhesions.
EMBO J. 19 5989-5999 (2000). 
 
4. EL TAHIR, Y. AND SKURNIK, M.
YadA, the multifaceted Yersinia adhesin.
INT.J.MED.MICROBIOL. 291 209-218 (2001).

Documentation
The genus Yersinia contains just three species: Y.enterocolita, Y.pestis, 
and Y.pseudotuberculosis [1]. Although the three use different routes to 
infect their host, each targets the lymphoid tissue for invasion, and all 
have developed specific systems to evade host immune cells [1]. PYV, a major 
virulence plasmid common to all members of this family, harbours the genes
necessary for type III secretion in the host and the exotoxins translocated 
by them. 
 
One of the proteins encoded within this plasmid is the Yersinia YadA 
non-fimbrial adhesin, a moiety that facilitates cell interaction between 
the host and pathogen [2]. Mutational studies indicate that this protein
allows intimate attachment and subsequent uptake by host macrophages of the 
bacterial cell. Synergistic mechanisms by two other PYV-encoded proteins,
YopH and YopE, inhibit the action of YadA. Electron microscopy of the 
mature YadA adhesins suggest that they form distinct "lollipop" shaped 
structures on the cell surface [3]. This is a trait shared by the adhesins
of another pathogen, namely Moraxella catarrhalis UspA1 and UspA2 [3]. 
 
The YadA protein itself exists as a homotrimer of 45kDa subunits, anchored 
in the outer bacterial membrane by its C-terminus [4]. The lollipop's 
globular head is formed by the N-terminus in the extracellular space. A
Yersinia bacterial cell thus coated with YadA can bind a number of host cell 
macromolecules, including collagen, laminin, mucus and fibronectin, 
enhancing its capacity for infection [4].
 
OMADHESIN is a 5-element fingerprint that provides a signature for the
Yersinia outer membrane adhesins. The fingerprint was derived from an
initial alignment of 5 sequences: the motifs were drawn from conserved 
regions spanning the N-terminal portion of the alignment (~400 amino 
acids). A single iteration on SPTR40_20f was required to reach convergence, 
no further sequences being identified beyond the starting set. A single
partial match was found, Q9ZGX0, a Yersinia hypothetical protein that failed
to match motifs 1, 2 and 5.
Summary Information
   5 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
555555
400000
300000
200110
12345
True Positives
O85267        Q56930        Q93KR4        YADA_YEREN    
YADA_YERPS
True Positive Partials
Codes involving 2 elements
Q9ZGX0
Sequence Titles
O85267      ADHESIN - Yersinia enterocolitica.            
Q56930 YOPA PROTEIN - Yersinia enterocolitica.
Q93KR4 ADHESIN YADA - Yersinia enterocolitica.
YADA_YEREN Invasin precursor (Outer membrane adhesin) - Yersinia enterocolitica.
YADA_YERPS Invasin precursor (Outer membrane adhesin) - Yersinia pseudotuberculosis.

Q9ZGX0 HYPOTHETICAL 7.6 KDA PROTEIN - Yersinia pestis.
Scan History
SPTR40_20f 1  100  NSINGLE    
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
TKDFKISVSAALISALF O85267 2 2 -
TKDFKISVSAALISALF YADA_YEREN 2 2 -
TKDFKISVSAALISALF Q56930 2 2 -
TKDFKISVSAALISALF Q93KR4 2 2 -
TKDFKISVSAALISALF YADA_YERPS 2 2 -

Motif 2 width=21
Element Seqn Id St Int Rpt
ASTAQKDGVAIGARASTSDTG O85267 120 101 -
ASTAQKDGVAIGARASTSDTG YADA_YEREN 120 101 -
ASTAQKDGVAIGARAFTSDTG Q56930 117 98 -
ASTAQKDGVAIGARAFTSDTG Q93KR4 117 98 -
SSTAQKDGVAIGARASASDTG YADA_YERPS 155 136 -

Motif 3 width=19
Element Seqn Id St Int Rpt
ENARKEAFAQSKDVLNMAK O85267 264 123 -
ENARKEAFAQSKDVLNMAK YADA_YEREN 264 123 -
ENARKEAFDLSNDALDMAK Q56930 246 108 -
ENARKEAFDLSNDALDMAK Q93KR4 246 108 -
ENARKETLAQSNDVLDAAK YADA_YERPS 258 82 -

Motif 4 width=16
Element Seqn Id St Int Rpt
DSKSSHTLKTANSYTD O85267 331 48 -
DSKSSHTLKTANSYTD YADA_YEREN 331 48 -
DSKSSHTLQTANSYTD Q56930 298 33 -
DSKSSHTLQTANSYTD Q93KR4 298 33 -
DSNSSHTLKTANSYTD YADA_YERPS 310 33 -

Motif 5 width=17
Element Seqn Id St Int Rpt
RESNQYTDHKFRQLDNR O85267 358 11 -
RESNQYTDHKFRQLDNR YADA_YEREN 358 11 -
RESNQYTDHKFRQLDNR Q56930 325 11 -
RESNQYTDHKFRQLDNR Q93KR4 325 11 -
SESNQYTDHKFSQLDNR YADA_YERPS 337 11 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
TKDFKISVSAALISALF O85267 2 2 -
TKDFKISVSAALISALF YADA_YEREN 2 2 -
TKDFKISVSAALISALF Q56930 2 2 -
TKDFKISVSAALISALF Q93KR4 2 2 -
TKDFKISVSAALISALF YADA_YERPS 2 2 -

Motif 2 width=21
Element Seqn Id St Int Rpt
ASTAQKDGVAIGARASTSDTG O85267 120 101 -
ASTAQKDGVAIGARASTSDTG YADA_YEREN 120 101 -
ASTAQKDGVAIGARAFTSDTG Q56930 117 98 -
ASTAQKDGVAIGARAFTSDTG Q93KR4 117 98 -
SSTAQKDGVAIGARASASDTG YADA_YERPS 155 136 -

Motif 3 width=19
Element Seqn Id St Int Rpt
ENARKEAFAQSKDVLNMAK O85267 264 123 -
ENARKEAFAQSKDVLNMAK YADA_YEREN 264 123 -
ENARKEAFDLSNDALDMAK Q56930 246 108 -
ENARKEAFDLSNDALDMAK Q93KR4 246 108 -
ENARKETLAQSNDVLDAAK YADA_YERPS 258 82 -

Motif 4 width=16
Element Seqn Id St Int Rpt
DSKSSHTLKTANSYTD O85267 331 48 -
DSKSSHTLKTANSYTD YADA_YEREN 331 48 -
DSKSSHTLQTANSYTD Q56930 298 33 -
DSKSSHTLQTANSYTD Q93KR4 298 33 -
DSNSSHTLKTANSYTD YADA_YERPS 310 33 -

Motif 5 width=17
Element Seqn Id St Int Rpt
RESNQYTDHKFRQLDNR O85267 358 11 -
RESNQYTDHKFRQLDNR YADA_YEREN 358 11 -
RESNQYTDHKFRQLDNR Q56930 325 11 -
RESNQYTDHKFRQLDNR Q93KR4 325 11 -
SESNQYTDHKFSQLDNR YADA_YERPS 337 11 -