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PR01754

Identifier
SACTRNSFRASE  [View Relations]  [View Alignment]  
Accession
PR01754
No. of Motifs
4
Creation Date
14-JUN-2002
Title
Streptothricin acetyltransferase signature 
Database References
Literature References
1. OELSCHLAEGER, T.A., DOBRINDT, U. AND HACKER, J. 
Virulence factors of uropathogens.
CURR.OPIN.UROL. 12 33-38 (2002).
 
2. SELTMANN, G. AND WOLTER, E.J.
Localization of a streptothricin acetyl transferase in cells of Escherichia 
coli K-12.
J.BASIC MICROBIOL. 25 197-2012 (1985).
 
3. OGASAWARA, N., NAKAI, S. AND YOSHIKAWA, H.
Systemic sequencing of the 180 kilobase region of the Bacillus subtilis 
chromosome containing the replication origin. 
DNA RES. 1 1-14 (1994).
 
4. FERNANDEZ-MORENO, M.A., VALLIN, C. AND MALPARTIDA, F.
Streptothricin biosynthesis is catalysed by enzymes related to nonribosomal 
peptide bond formation. 
J.BACTERIOL. 179 6929-6936 (1997).

Documentation
A small number of bacterial pathogens are implicated in urinary tract
infections (UTIs), amongst the most frequent infections in the developed
world. The commonest bacterium isolated from UTI is Escherichia coli, with 
streptococcal and staphylococcal species coming a close second [1]. Virulent 
microbes that colonise the human urinary tract usually possess sets of
virulence factors specific to the host environment [1]. The most common are
adhesins, molecules that allow an infection to become established; well-
characterised E.coli type I pili are a good example.
 
Aside from adhesins, other UTI-specific virulence moieties include: toxins, 
such as Cnf1 and hemolysin, and host biocides that act against other
microbes competing for the same niche [1]. Streptothricin, an antibiotic 
synthesised and secreted by some Gram-negative pathogens, is an example of 
the latter [2]; the antibiotic also has a toxic effect on host cells. The
biocide is synthesised in a five-step process in the bacterial cytoplasm,
and secreted to the cell exterior via the general secretory pathway [2,3]. 
 
The last step in the synthesis process is the acetyl co-enzyme A-dependent 
acetylation of the streptothricin molecule to the mature antibiotic. This is 
catalysed by the streptothricin acetyltransferase protein, located adjacent 
to the inner face of the cytoplasmic membrane [2,3]. Homologues of the 
original gene found in Streptomyces spp. have been found in Bacillus 
subtilis and Staphylococcus spp., as well as E.coli [2,3]. More recently, 
the streptothricin biosynthesis enzymes were shown to be related to those
that carry out non-ribosomal peptide bond formation [4].
 
SACTRNSFRASE is a 4-element fingerprint that provides a signature for the
streptothricin acetyltransferases. The fingerprint was derived from an
initial alignment of 5 sequences: the motifs were drawn from conserved 
regions spanning the C-terminal portion of the alignment (~90 amino acids).
Two iterations on SPTR40_20f were required to reach convergence, at which
point a true set comprising 6 sequences was identified. Three partial 
matches were also found, Q47625, O33583 and Q08414, all of which are related
streptothricin/nourseothricin acetyltransferases that match two motifs. 
Summary Information
   6 codes involving  4 elements
0 codes involving 3 elements
3 codes involving 2 elements
Composite Feature Index
46666
30000
22130
1234
True Positives
Q45970        Q57025        Q9AL03        Q9EZB9        
STA_ECOLI YYAR_BACSU
True Positive Partials
Codes involving 2 elements
O33583 Q08414 Q47625
Sequence Titles
Q45970      STREPTOTHRICINE-ACETYL-TRANSFERASE (EC 2.3.1.) - Campylobacter coli. 
Q57025 STREPTOTHRICIN-ACETYLTRANSFERASE - Escherichia coli, and unidentified.
Q9AL03 STREPTOTHRICIN ACETYLTRANSFERASE - Enterococcus faecalis (Streptococcus faecalis), and Enterococcus faecium (Streptococcus faecium).
Q9EZB9 STREPTOTHRICIN ACETYLTRANSFERASE - Staphylococcus intermedius.
STA_ECOLI Streptothricin acetyltransferase (EC 2.3.-.-) - Escherichia coli.
YYAR_BACSU Hypothetical 20.4 kDa protein in COTF-TETB intergenic region - Bacillus subtilis.

O33583 NOURSEOTHRICIN ACETYLTRANSFERASE - Streptomyces noursei.
Q08414 NOURSEOTHRICIN ACETYLTRANSFERASE (EC 2.3.1.-) - Streptomyces noursei.
Q47625 STREPTOTHRICIN-ACTEYL-TRANSFERASE - Escherichia coli.
Scan History
SPTR40_20f 2  75   NSINGLE    
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
EDIAVCKDFRGQGIGS Q45970 100 100 -
EDIAVCKDFRGQGIGS Q9EZB9 96 96 -
EDIAVCKDFRGQGIGS Q9AL03 100 100 -
EDIAVAKDYRKKGVGT YYAR_BACSU 93 93 -
EHIVVSHTHRGKGVAH STA_ECOLI 87 87 -

Motif 2 width=16
Element Seqn Id St Int Rpt
WAKHKNLHGLMLETQD Q45970 124 8 -
WAKHKNLHGLMLETQD Q9EZB9 120 8 -
WAKHKNLHGLMLETQD Q9AL03 124 8 -
WAKENHFCGLMLETQD YYAR_BACSU 117 8 -
WALSRQLLGIRLETQT STA_ECOLI 111 8 -

Motif 3 width=17
Element Seqn Id St Int Rpt
CKFYHNCGFKIGSVDTM Q45970 145 5 -
CKFYHNCGFKIGSVDTM Q9EZB9 141 5 -
CKFYHNCGFKIGSVDTM Q9AL03 145 5 -
CHFYAKHHFIIGAVDTM YYAR_BACSU 138 5 -
CNLYAKCGFTLGGIDLF STA_ECOLI 132 5 -

Motif 4 width=14
Element Seqn Id St Int Rpt
ENNFEKAVFWYLRF Q45970 167 5 -
ENNFEKAVFWYLRF Q9EZB9 163 5 -
ENNFEKAVFWYLRF Q9AL03 167 5 -
PTANEIAIFWYYKF YYAR_BACSU 160 5 -
QVSNETAMYWYWFS STA_ECOLI 155 6 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
EDIAVCKDFRGQGIGS Q45970 100 100 -
EDIAVCKDFRGQGIGS Q9EZB9 96 96 -
EDIAVCKDFRGQGIGS Q9AL03 100 100 -
EDIAVAKDYRKKGVGT YYAR_BACSU 93 93 -
DDIAVDVPYRGSGVSR Q57025 99 99 -
EHIVVSHTHRGKGVAH STA_ECOLI 87 87 -

Motif 2 width=16
Element Seqn Id St Int Rpt
WAKHKNLHGLMLETQD Q45970 124 8 -
WAKHKNLHGLMLETQD Q9EZB9 120 8 -
WAKHKNLHGLMLETQD Q9AL03 124 8 -
WAKENHFCGLMLETQD YYAR_BACSU 117 8 -
WARNVPSAGVRLETQS Q57025 123 8 -
WALSRQLLGIRLETQT STA_ECOLI 111 8 -

Motif 3 width=17
Element Seqn Id St Int Rpt
CKFYHNCGFKIGSVDTM Q45970 145 5 -
CKFYHNCGFKIGSVDTM Q9EZB9 141 5 -
CKFYHNCGFKIGSVDTM Q9AL03 145 5 -
CHFYAKHHFIIGAVDTM YYAR_BACSU 138 5 -
CRFYRRYGFRLGGYDRY Q57025 144 5 -
CNLYAKCGFTLGGIDLF STA_ECOLI 132 5 -

Motif 4 width=14
Element Seqn Id St Int Rpt
ENNFEKAVFWYLRF Q45970 167 5 -
ENNFEKAVFWYLRF Q9EZB9 163 5 -
ENNFEKAVFWYLRF Q9AL03 167 5 -
PTANEIAIFWYYKF YYAR_BACSU 160 5 -
PGSREVALFWYLSF Q57025 167 6 -
QVSNETAMYWYWFS STA_ECOLI 155 6 -