SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR01753

Identifier
STREPKINASE  [View Relations]  [View Alignment]  
Accession
PR01753
No. of Motifs
12
Creation Date
13-JUN-2002
Title
Streptococcus streptokinase protein signature
Database References

PDB; 1C4P
SCOP; 1C4P
CATH; 1C4P
Literature References
1. AWADA, A., VAN DER AUWERA, P., MEUNIER, F., DANEAU, D. AND KLASTERSKY, J.
Streptococcal and enterococcal bacteremia in patients with cancer. 
CLIN.INFECT.DIS 15 33-48 (1992).
 
2. BEN NASR, A., WISTEDT, A., RINGDAHL, U. AND SJOBRING, U.
Streptokinase activates plasminogen bound to human group C and G 
streptococci through M-like protein.
EUR.J.BIOCHEM. 222 267-276 (1994). 
 
3. TEWODROS, W., NOGREN, M. AND KRONVALL, G.
Streptokinase activity among group A streptococci in relation to 
streptokinase genotype, plasminogen binding and disease manifestations.
MICROB.PATHOG. 18 53-65 (1995). 
 
4. WANG, H., LOTTENBERG, R. AND BOYLE, M.D. 
Analysis of the interaction of group A streptococci with fibrinogen, 
streptokinase and plasminogen.
MICROB.PATHOG. 18 153-166 (1995). 
 
5. WANG, X., TANG, J., HUNTER, B. AND ZHANG, X.C.
Crystal structure of streptokinase beta-domain.
FEBS LETT. 459 85-89 (1999).

Documentation
Streptococcal species and strains can be divided into groups, depending 
on the action of their hemolysis on blood agar, serological activity, 
biochemical reactions and antigenic variations. They are Gram-positive cocci 
that occur naturally in pairs or chains, and most are facultative anaerobes 
[1]. Streptococci occur as part of the natural human flora, but may turn 
pathogenic if transformed by the addition of virulence factors to the 
microbial genome [1].
 
Virulent proteins expressed by the pathogenic strains of Streptococcus spp. 
include the M protein antigen and pyrogenic exotoxins of S.pyogenes, the 
hyaluronic capsule of S.pneumoniae, and various secreted extracellular 
enzymes common to most of the virulent streptococci [2]. Streptokinase, an 
example of the latter, is secreted upon invasion of the host tissues by 
the bacterium and binds to host plasminogen, recruiting phagocytic cells to 
the site of infection [2]. The activity of this virulence factor has been
linked to acute post-streptococcal glomerulonephritis, a disease of the 
kidneys in children and the immunosuppressed [3].
 
Group A streptococci possess a particularly strong form of streptokinase 
that can bind not only plasminogen, but also host fibrinogen [4]. This 
close interaction with the human fibrinolytic system allows the microbe to 
acquire unregulatable cell-surface enzymatic activity, promoting further 
spread from the site of infection. The complexes formed between 
streptococcal cells, streptokinase, plasminogen and fibrinogen can lyse 
fibrin clots in the host [4]. 
 
The structure of the beta-domain of S.equisimilis streptokinase has been
determined to 2.4A by means of X-ray crystallography [5]. The fold is
characterised by a putative binding site for a plasminogen kringle domain
at the tip of an exposed hairpin loop. It is thought that streptokinase
proteins may exist as dimers, both when anchored to the bacterial membrane
and in solution [5].
 
STREPKINASE is a 12-element fingerprint that provides a signature for the
Streptococcus streptokinase protein family. The fingerprint was derived from
an initial alignment of 4 sequences: the motifs were drawn from conserved
regions spanning the N-terminal portion of the alignment (~390 amino acids)
- motif 5 encodes the N-terminus of beta-strand 1; motif 6 spans the 
N-terminus of strand 2; motif 7 encompasses the C-terminus of alpha-helix 1 
and the N-terminus of strand 4; motif 8 spans strand 6 and the N-terminus 
of strand 7; and motif 9 encodes the C-terminus of strand 10. Two iterations 
on SPTR40_20f were required to reach convergence, at which point a true set 
comprising 6 sequences was identified.
Summary Information
6 codes involving 12 elements
0 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
12666666666666
11000000000000
10000000000000
9000000000000
8000000000000
7000000000000
6000000000000
5000000000000
4000000000000
3000000000000
2000000000000
123456789101112
True Positives
Q53284        Q57391        STRP_STREQ    STRP_STRPY    
STRP_STRSP STRQ_STRPY
Sequence Titles
Q53284      SKC-2 - Streptococcus equisimilis.            
Q57391 GROUP A STREPTOKINASE PRECURSOR - Streptococcus pyogenes.
STRP_STREQ Streptokinase C precursor - Streptococcus equisimilis.
STRP_STRPY Streptokinase A precursor - Streptococcus pyogenes.
STRP_STRSP Streptokinase G precursor - Streptococcus sp. (strain 19909).
STRQ_STRPY Streptokinase precursor - Streptococcus pyogenes.
Scan History
SPTR40_20f 2  75   NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
GPEWLLDRPSVNNSQ STRP_STRSP 29 29 -
GPEWLLDRPSVNNSQ STRP_STREQ 29 29 -
GYGWLPDRPPINNSQ STRP_STRPY 29 29 -
GPEWLLGRPSVNNSQ STRQ_STRPY 29 29 -

Motif 2 width=17
Element Seqn Id St Int Rpt
FFEIDLTSRPAHGGKTE STRP_STRSP 63 19 -
FFEIDLTSRPAHGGKTE STRP_STREQ 63 19 -
FFEIDLTSQPAHGGKTE STRP_STRPY 63 19 -
FFEIDLTSRPAQGGKTE STRQ_STRPY 63 19 -

Motif 3 width=17
Element Seqn Id St Int Rpt
HSNDDYFEVIDFASDAT STRP_STRSP 118 38 -
HSNDDYFEVIDFASDAT STRP_STREQ 118 38 -
HSNDGYFEVIDFASDAT STRP_STRPY 118 38 -
HSNDGYFEVIDFASDAT STRQ_STRPY 118 38 -

Motif 4 width=18
Element Seqn Id St Int Rpt
DRNGKVYFADKDGSVTLP STRP_STRSP 137 2 -
DRNGKVYFADKDGSVTLP STRP_STREQ 137 2 -
DRNGKVYFADKDGSVTLP STRP_STRPY 137 2 -
DRNGKVYFADRDDSVTLP STRQ_STRPY 137 2 -

Motif 5 width=16
Element Seqn Id St Int Rpt
RPYKEKPVQNQAKSVD STRP_STRSP 170 15 -
RPYKEKPIQNQAKSVD STRP_STREQ 170 15 -
RPYKEKPVQNQAKSVD STRP_STRPY 170 15 -
RPYRPKAVHNSAERVN STRQ_STRPY 170 15 -

Motif 6 width=17
Element Seqn Id St Int Rpt
PDDDFRPALKDTKLLKT STRP_STRSP 197 11 -
PDDDFRPGLKDTKLLKT STRP_STREQ 197 11 -
PDDDFRPGLKDTKLLKT STRP_STRPY 197 11 -
GNLDFTPSLKEQYHLTT STRQ_STRPY 197 11 -

Motif 7 width=15
Element Seqn Id St Int Rpt
NKNHPGYTIYERDSS STRP_STRSP 234 20 -
NKNHPGYTIYERDSS STRP_STREQ 234 20 -
NKTHPGYTIYERDSS STRP_STRPY 234 20 -
SKKHPDYIITKRDSS STRQ_STRPY 234 20 -

Motif 8 width=18
Element Seqn Id St Int Rpt
PMDQEFTYHVKNREQAYR STRP_STRSP 262 13 -
PMDQEFTYRVKNREQAYR STRP_STREQ 262 13 -
PMDQEFTYHVKNREQAYE STRP_STRPY 262 13 -
PMDQEFTYHIKDREQAYK STRQ_STRPY 262 13 -

Motif 9 width=20
Element Seqn Id St Int Rpt
SEKYYVLKKGEKPYDPFDRS STRP_STRSP 297 17 -
SEKYYVLKKGEKPYDPFDRS STRP_STREQ 297 17 -
SEKYYVLKQGEKPYDPFDRS STRP_STRPY 297 17 -
SEKYYILKKGEKPYDPFDRS STRQ_STRPY 297 17 -

Motif 10 width=15
Element Seqn Id St Int Rpt
ERNLDFRDLYDPRDK STRP_STRSP 344 27 -
ERNLDFRDLYDPRDK STRP_STREQ 344 27 -
ERNLDFRDLYDPRDK STRP_STRPY 344 27 -
ERNLDFRDLYDPRDK STRQ_STRPY 344 27 -

Motif 11 width=17
Element Seqn Id St Int Rpt
DYTLTGKVEDNHDDTNR STRP_STRSP 373 14 -
DYTLTGKVEDNHDDTNR STRP_STREQ 373 14 -
DYTLTGKVEDNHDKNNR STRP_STRPY 373 14 -
GYTLTGKVEDNHDDTNR STRQ_STRPY 373 14 -

Motif 12 width=16
Element Seqn Id St Int Rpt
YDKDRYTEEEREVYSY STRP_STRSP 410 20 -
YDKDRYTEEEREVYSY STRP_STREQ 410 20 -
YDKDLYTEEERKAYSY STRP_STRPY 410 20 -
YDKDRYTEEEREVYSY STRQ_STRPY 410 20 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
GPEWLLDRPSVNNSQ STRP_STRSP 29 29 -
GPEWLLDRPSVNNSQ Q53284 3 3 -
GPEWLLDRPSVNNSQ STRP_STREQ 29 29 -
GYGWLPDRPPINNSQ STRP_STRPY 29 29 -
GYEWLLDRPSVNNSQ Q57391 29 29 -
GPEWLLGRPSVNNSQ STRQ_STRPY 29 29 -

Motif 2 width=17
Element Seqn Id St Int Rpt
FFEIDLTSRPAHGGKTE STRP_STRSP 63 19 -
FFEIDLTSRPAHGGKTE Q53284 37 19 -
FFEIDLTSRPAHGGKTE STRP_STREQ 63 19 -
FFEIDLTSQPAHGGKTE STRP_STRPY 63 19 -
FFEIDLTSRPAHGGKTE Q57391 63 19 -
FFEIDLTSRPAQGGKTE STRQ_STRPY 63 19 -

Motif 3 width=17
Element Seqn Id St Int Rpt
HSNDDYFEVIDFASDAT STRP_STRSP 118 38 -
HSNDDYFEVIDFASDAT Q53284 92 38 -
HSNDDYFEVIDFASDAT STRP_STREQ 118 38 -
HSNDGYFEVIDFASDAT STRP_STRPY 118 38 -
HSNDGYFEVIDFASDAT Q57391 118 38 -
HSNDGYFEVIDFASDAT STRQ_STRPY 118 38 -

Motif 4 width=18
Element Seqn Id St Int Rpt
DRNGKVYFADKDGSVTLP STRP_STRSP 137 2 -
DRNGKVYFADKDGSVTLP Q53284 111 2 -
DRNGKVYFADKDGSVTLP STRP_STREQ 137 2 -
DRNGKVYFADKDGSVTLP STRP_STRPY 137 2 -
DRNGKVYFADKDDSVTLP Q57391 137 2 -
DRNGKVYFADRDDSVTLP STRQ_STRPY 137 2 -

Motif 5 width=16
Element Seqn Id St Int Rpt
RPYKEKPVQNQAKSVD STRP_STRSP 170 15 -
RPYKEKPIQNQAKSVD Q53284 144 15 -
RPYKEKPIQNQAKSVD STRP_STREQ 170 15 -
RPYKEKPVQNQAKSVD STRP_STRPY 170 15 -
KPYQPKAVHNSAERVN Q57391 170 15 -
RPYRPKAVHNSAERVN STRQ_STRPY 170 15 -

Motif 6 width=17
Element Seqn Id St Int Rpt
PDDDFRPALKDTKLLKT STRP_STRSP 197 11 -
PDDDFRPGLKDTKLLKT Q53284 171 11 -
PDDDFRPGLKDTKLLKT STRP_STREQ 197 11 -
PDDDFRPGLKDTKLLKT STRP_STRPY 197 11 -
GDLDFTPLLRNQYHLTT Q57391 197 11 -
GNLDFTPSLKEQYHLTT STRQ_STRPY 197 11 -

Motif 7 width=15
Element Seqn Id St Int Rpt
NKNHPGYTIYERDSS STRP_STRSP 234 20 -
NKTHPGYTIYERDSS Q53284 208 20 -
NKNHPGYTIYERDSS STRP_STREQ 234 20 -
NKTHPGYTIYERDSS STRP_STRPY 234 20 -
SKKHPDYIITKRDSS Q57391 234 20 -
SKKHPDYIITKRDSS STRQ_STRPY 234 20 -

Motif 8 width=18
Element Seqn Id St Int Rpt
PMDQEFTYHVKNREQAYR STRP_STRSP 262 13 -
PMDQEFTYHVKNREQAYE Q53284 236 13 -
PMDQEFTYRVKNREQAYR STRP_STREQ 262 13 -
PMDQEFTYHVKNREQAYE STRP_STRPY 262 13 -
PMDQEFTYHIKDREQAYK Q57391 262 13 -
PMDQEFTYHIKDREQAYK STRQ_STRPY 262 13 -

Motif 9 width=20
Element Seqn Id St Int Rpt
SEKYYVLKKGEKPYDPFDRS STRP_STRSP 297 17 -
SEKYYVLKKGEKPYDPFDRS Q53284 271 17 -
SEKYYVLKKGEKPYDPFDRS STRP_STREQ 297 17 -
SEKYYVLKQGEKPYDPFDRS STRP_STRPY 297 17 -
SEKYYVLKKGEKPYDPFDRS Q57391 297 17 -
SEKYYILKKGEKPYDPFDRS STRQ_STRPY 297 17 -

Motif 10 width=15
Element Seqn Id St Int Rpt
ERNLDFRDLYDPRDK STRP_STRSP 344 27 -
ERNLDFRDLYDPRDK Q53284 318 27 -
ERNLDFRDLYDPRDK STRP_STREQ 344 27 -
ERNLDFRDLYDPRDK STRP_STRPY 344 27 -
ERNLDFRDLYDPRDK Q57391 344 27 -
ERNLDFRDLYDPRDK STRQ_STRPY 344 27 -

Motif 11 width=17
Element Seqn Id St Int Rpt
DYTLTGKVEDNHDDTNR STRP_STRSP 373 14 -
DYTLTGKVEDNHDDTNR Q53284 347 14 -
DYTLTGKVEDNHDDTNR STRP_STREQ 373 14 -
DYTLTGKVEDNHDKNNR STRP_STRPY 373 14 -
DYTLTGKVEDNHDDTNR Q57391 373 14 -
GYTLTGKVEDNHDDTNR STRQ_STRPY 373 14 -

Motif 12 width=16
Element Seqn Id St Int Rpt
YDKDRYTEEEREVYSY STRP_STRSP 410 20 -
YDKDRYTEEEREVYSY Q53284 384 20 -
YDKDRYTEEEREVYSY STRP_STREQ 410 20 -
YDKDLYTEEERKAYSY STRP_STRPY 410 20 -
YDKDRYTEEEREVYSY Q57391 410 20 -
YDKDRYTEEEREVYSY STRQ_STRPY 410 20 -